A biodegradable gene transfer vector, poly(ethylenimine)-grafted-poly[(aspartic acid)-co-lysine] has been developed by thermal polycondensation of aspartic acid and lysine, and branch poly(ethylenimine) (Mw less than ...A biodegradable gene transfer vector, poly(ethylenimine)-grafted-poly[(aspartic acid)-co-lysine] has been developed by thermal polycondensation of aspartic acid and lysine, and branch poly(ethylenimine) (Mw less than 600) was grafted to the backbone. The polymer was characterized by 1H NMR. It appeared lower cytotoxity compared to poly(ethylenimine) (25KDa), which was quantified by MTT assay. Electrophoresis indicated that the polymer could retardate DNA at N/P ratio 1.2-1.8 (w/w). Transfection efficiency of the complexes was studied in NT2 cell lines. It was 1.5 fold higher than molecular weight PEI (Mw = 25KDa).展开更多
Due to a lack of technology,smaller breweries simply dump excess CO2 into the atmosphere,fueling the greenhouse effect and global warming.State-of-the-art CO2 capture technologies using nanofibrillated cellulose are e...Due to a lack of technology,smaller breweries simply dump excess CO2 into the atmosphere,fueling the greenhouse effect and global warming.State-of-the-art CO2 capture technologies using nanofibrillated cellulose are expensive and require laborious freeze-drying.Consequently,there is a high demand for affordable alternatives in order to reduce the environmental impact in this industry sector.This work describes a novel route for a quick and cost-efficient synthesis of amine-functionalized cellulose pellets by a surfactantassisted steam explosion process.Typical values with this method were porosity of 92%and density of 67 g/cm^3.Investigations on polyethylenimine(PEI)content and distribution revealed a maximum PEI concentration of 20 wt%with decreasing concentration to the core of a pellet.Sufficient stability against brewery exhaust gas was determined and CO2 release at^120℃ could be confirmed.Capacity tests under simulated working conditions with a novel laboratory reactor yielded a CO2 capacity of 1.0 mmol/g or 67 mol/m^3,which is comparable to values known from the literature for other cellulose-based adsorbents.展开更多
文摘A biodegradable gene transfer vector, poly(ethylenimine)-grafted-poly[(aspartic acid)-co-lysine] has been developed by thermal polycondensation of aspartic acid and lysine, and branch poly(ethylenimine) (Mw less than 600) was grafted to the backbone. The polymer was characterized by 1H NMR. It appeared lower cytotoxity compared to poly(ethylenimine) (25KDa), which was quantified by MTT assay. Electrophoresis indicated that the polymer could retardate DNA at N/P ratio 1.2-1.8 (w/w). Transfection efficiency of the complexes was studied in NT2 cell lines. It was 1.5 fold higher than molecular weight PEI (Mw = 25KDa).
文摘Due to a lack of technology,smaller breweries simply dump excess CO2 into the atmosphere,fueling the greenhouse effect and global warming.State-of-the-art CO2 capture technologies using nanofibrillated cellulose are expensive and require laborious freeze-drying.Consequently,there is a high demand for affordable alternatives in order to reduce the environmental impact in this industry sector.This work describes a novel route for a quick and cost-efficient synthesis of amine-functionalized cellulose pellets by a surfactantassisted steam explosion process.Typical values with this method were porosity of 92%and density of 67 g/cm^3.Investigations on polyethylenimine(PEI)content and distribution revealed a maximum PEI concentration of 20 wt%with decreasing concentration to the core of a pellet.Sufficient stability against brewery exhaust gas was determined and CO2 release at^120℃ could be confirmed.Capacity tests under simulated working conditions with a novel laboratory reactor yielded a CO2 capacity of 1.0 mmol/g or 67 mol/m^3,which is comparable to values known from the literature for other cellulose-based adsorbents.
