Objective:To identify the major organic compounds of Artemisia austeralis(A.australis),a plant used in traditional Hawaiian medicine for the treatment of asthma.Methods:The dichloromethane extract of A.australis was a...Objective:To identify the major organic compounds of Artemisia austeralis(A.australis),a plant used in traditional Hawaiian medicine for the treatment of asthma.Methods:The dichloromethane extract of A.australis was analyzed by gas chromatography—mass spectroscopy and major compounds were identified by a National Institute of Standards and Technology library search and confirmed by peak enhancement Results:The major chemical components of A.australis include eucalyptol.borneol,and caryophyllene.Conclusions:The presence and biological activity of eucalyptol correlate very well with the usage of this plant in traditional Hawaiian medicine.展开更多
Current antiepileptic drugs can inhibit seizure occurrence but are not effective in preventing its onset.Moreover,they produce several side effects,which may impact the efficacy of the treatment.In addition,it has bee...Current antiepileptic drugs can inhibit seizure occurrence but are not effective in preventing its onset.Moreover,they produce several side effects,which may impact the efficacy of the treatment.In addition,it has been stimulating the prospection of new molecules isolated from aromatic plants,with potential anticonvulsant and neuroprotective activities and less side effects.This study aimed to evaluate,though behavioural and neuro-chemical methodologies,the anticonvulsant,and neuroprotective effects of eucalyptol on mice subjected to the pilocarpine-induced seizure model.Eucalyptol(100,200 and 400 mg/kg,p.o.)was administered in mice prior to pilocarpine(350 mg/kg,i.p.)and the following behavioural parameters were assessed:Latency to First Seizure(LFS),Seizure Intensity(SI)and Latency to Death(LD).In addition,an oxotremorine-induced tremors test was performed to evaluate the cholinergic system involvement on the eucalyptol effects.Neurochemical tests were also performed,including determination of hippocampal(HC)concentration of thiobarbituric acid reactive substances(TBARS)and nitrite/nitrate and striatal(ST)concentration of noradrenaline,dopamine,and sero-tonin.Lastly,histopathological,and morphometric hippocampal analysis were conducted.Eucalyptol increased the latency to first seizure and latency to death,inhibited oxotremorine-induced tremors,decreased hippocampal TBARS and nitrite/nitrate overproduction,increased striatal noradrenaline and dopamine levels and prevented hippocampal neurodegeneration.These results demonstrate the potential anticonvulsant,neuroprotective and antioxidant effects of eucalyptol,probably through a conjunction of mechanisms including muscarinic cholin-ergic antagonism,oxidative stress mitigation and the monoaminergic system modulation,which appears to effectively control the seizure onset.展开更多
基金Supported by the student mentored research fund at Brigham Young University-Hawaii(Grant No.10-634200-7210)
文摘Objective:To identify the major organic compounds of Artemisia austeralis(A.australis),a plant used in traditional Hawaiian medicine for the treatment of asthma.Methods:The dichloromethane extract of A.australis was analyzed by gas chromatography—mass spectroscopy and major compounds were identified by a National Institute of Standards and Technology library search and confirmed by peak enhancement Results:The major chemical components of A.australis include eucalyptol.borneol,and caryophyllene.Conclusions:The presence and biological activity of eucalyptol correlate very well with the usage of this plant in traditional Hawaiian medicine.
基金supported by the Cearense Foundation for Support to Scientific(Fundaçāo Cearense de Apoio ao Desenvolvimento Científico e Tecnológico)[grant number:BMD-0008-01254.01.16/18,2018]the Regional University of Cariri.
文摘Current antiepileptic drugs can inhibit seizure occurrence but are not effective in preventing its onset.Moreover,they produce several side effects,which may impact the efficacy of the treatment.In addition,it has been stimulating the prospection of new molecules isolated from aromatic plants,with potential anticonvulsant and neuroprotective activities and less side effects.This study aimed to evaluate,though behavioural and neuro-chemical methodologies,the anticonvulsant,and neuroprotective effects of eucalyptol on mice subjected to the pilocarpine-induced seizure model.Eucalyptol(100,200 and 400 mg/kg,p.o.)was administered in mice prior to pilocarpine(350 mg/kg,i.p.)and the following behavioural parameters were assessed:Latency to First Seizure(LFS),Seizure Intensity(SI)and Latency to Death(LD).In addition,an oxotremorine-induced tremors test was performed to evaluate the cholinergic system involvement on the eucalyptol effects.Neurochemical tests were also performed,including determination of hippocampal(HC)concentration of thiobarbituric acid reactive substances(TBARS)and nitrite/nitrate and striatal(ST)concentration of noradrenaline,dopamine,and sero-tonin.Lastly,histopathological,and morphometric hippocampal analysis were conducted.Eucalyptol increased the latency to first seizure and latency to death,inhibited oxotremorine-induced tremors,decreased hippocampal TBARS and nitrite/nitrate overproduction,increased striatal noradrenaline and dopamine levels and prevented hippocampal neurodegeneration.These results demonstrate the potential anticonvulsant,neuroprotective and antioxidant effects of eucalyptol,probably through a conjunction of mechanisms including muscarinic cholin-ergic antagonism,oxidative stress mitigation and the monoaminergic system modulation,which appears to effectively control the seizure onset.
