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ANALYSIS OF EVOLUTIONARY RELATIONSHIP BETWEEN ASTASIA LONGA AND EUGLENA GRACILIS BY USING RAPD TECHNIQUE AND CLADISTIC ANALYSIS 被引量:1
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作者 王江新 施之新 +1 位作者 甘小妮 谢树莲 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2001年第1期40-50,共11页
Although both Astasia longa and Euglena gracilis belong to different genera, they share many morphological characters except that A. longa has no chloroplast. In the 1940’s, on the basis of the finding that in darkne... Although both Astasia longa and Euglena gracilis belong to different genera, they share many morphological characters except that A. longa has no chloroplast. In the 1940’s, on the basis of the finding that in darkness or upon addition of some chemicals, E. gracilis would fade reversibly or irreversibly, some scholars hypothesised that A. longa evolved from E. gracilis by losing chloroplast. The authors’ use of RAPD and cladistic analyses in a study on the evolutionary relationship between A .longa and E. gracilis showed that the A. longa ’s relationship with E. gracilis was closer than that with other green euglenoids. This proves the hypothesis that A. longa evolved from E. gracilis is reasonable. The results of this study suggest that saprophytic colorless euglenoids were transformed from green euglenoids by losing their choroplasts. 展开更多
关键词 A. longa euglena gracilis cladistic analysis molecular evolution RAPD
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Occurrence and light response of residual plastid genes in a Euglena gracilis bleached mutant strain OflB2
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作者 QIN Huan GUO Qingqing +5 位作者 LIU Chenchen LI Fenglan ZHANG Hua CHU Zihan WANG Jiangxin LEI Anping 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2020年第6期1858-1866,共9页
Euglena gracilis is a unicellular green eukaryotic microalga that features characteristics of both plants and animals.The photosynthetic function of its chloroplast is easily lost under stress resulting in bleached mu... Euglena gracilis is a unicellular green eukaryotic microalga that features characteristics of both plants and animals.The photosynthetic function of its chloroplast is easily lost under stress resulting in bleached mutants,while the physiological role of their residual plastid DNAs remains unclear.In this study,we obtained five bleached mutants by ofloxacin(Ofl)treatment,identified 12 residual plastid genes in five bleached mutants,and determined the mRNA levels in the wild type E.gracilis(WT)and one bleached mutant(OflB2)under dark and light stimulation conditions by quantitative reverse transcribed PCR(qRTPCR).Results show that the expression of all selected plastid genes in both WT and OflB2 mutant did not change significantly in darkness,while their responses to light stimulation were different.Under the light stimulation conditions,half of the genes did not change significantly,while most of the other genes were down-regulated in OflB2 mutant and up-regulated in WT.Therefore,the bleached mutant retains part of the plastid genome and the plastid relic is responsive to light.Our research will help to understand the functions of residual plastid DNA and evolution of chloroplasts. 展开更多
关键词 euglena gracilis bleached mutant residual plastid genes light response
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Hydrodynamics of Euglena Gracilis during locomotion
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作者 Fahrettin Gokhan Ergin 《Journal of Hydrodynamics》 SCIE EI CSCD 2021年第1期120-126,共7页
The hydrodynamics and locomotion mechanism of Euglena Gracilis(E.Gracilis)is investigated using microscopic shadow imaging and micro particle image velocimetry(MicroPIV).Three distinct locomotion modes were observed:t... The hydrodynamics and locomotion mechanism of Euglena Gracilis(E.Gracilis)is investigated using microscopic shadow imaging and micro particle image velocimetry(MicroPIV).Three distinct locomotion modes were observed:translation,spin,and left/right turn.Since the flagellum was not possible to image,the strokes were identified by evaluating the flow field around the protist.The flow field information is obtained using a phase-separated PIV evaluation,which uses a histogram-thresholding based dynamic masking approach.The temporal resolution of the experiment was sufficient to identify the sequence of two modes,translation and spin,and the stroke-pulling frequency.The flow field result during a stroke is compared with existing Stokeslet dipole theories and flow disturbance decay with distance is investigated.The results indicate that the organism has a complex locomotion technique that allows the change of direction,axial orientation and propulsion. 展开更多
关键词 euglena gracilis(E.gracilis)locomotion micro particle image velocimetry(MicroPIV) dynamic masking
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γ-Irradiation Effect:Variation of Photosynthetic Activity of Euglena
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作者 T·SAKASHITA M·DOI 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2002年第3期261-267,共7页
Objective To study the effects of gamma-ray irradiation on carbon fixation (Specific production rate: SPR), CO2 utilization efficiency (CUE) and electron transfer rate (ETR) in the photosynthetic flagellate Euglena g... Objective To study the effects of gamma-ray irradiation on carbon fixation (Specific production rate: SPR), CO2 utilization efficiency (CUE) and electron transfer rate (ETR) in the photosynthetic flagellate Euglena gracilis strain Z in a dose-response dependent manner. Methods Euglena cells were cultured in an inorganic nutrient medium containing ammonium chloride or proteose peptone. Cells were exposed to gamma-ray at 5 doses (0, 100, 250, 350, 500 Gy for water). Five days after irradiation, three photosynthetic activities were measured. SPR, which is a carbon uptake rate per unit carbon mass, was determined by 13C tracer methodology. CUE was evaluated using a relation of carbon isotope fractionation in Calvin cycle. ETR in photosystem II (PS II) was measured by a chlorophyll fluorescence analysis. Results Even at a dose of 500 Gy, 80 % of ETR of the non-irradiated control (0 Gy) was sustained, while SPR and CUE were about half the level in the non-irradiated control at 500 Gy. Furthermore, the dose response of ETR was considerably different from the others. Conclusion Our findings suggest that not only PS II but also the Calvin cycle in the photosynthetic system is affected by gamma ray irradiation. 展开更多
关键词 Gamma-ray irradiation euglena gracilis Photosynthetic activities
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