目的观察姜黄素对缺氧致小鼠视网膜神经节细胞RGC-5氧化应激损伤的影响,并探讨其作用机制。方法以小鼠RGC-5细胞株为研究对象,按照不同处理因素将细胞随机分为4组:正常对照组、缺氧组、缺氧+溶剂(二甲基亚砜)对照组、缺氧+姜黄素预处理...目的观察姜黄素对缺氧致小鼠视网膜神经节细胞RGC-5氧化应激损伤的影响,并探讨其作用机制。方法以小鼠RGC-5细胞株为研究对象,按照不同处理因素将细胞随机分为4组:正常对照组、缺氧组、缺氧+溶剂(二甲基亚砜)对照组、缺氧+姜黄素预处理组。2′7′-二氯荧光素探针法检测细胞内活性氧自由基(ROS),四甲基偶氮唑蓝法检测细胞增殖活性,Western blot检测细胞内核转录因子Nrf2蛋白的表达,实时荧光定量RT-PCR检测醌氧化还原酶(NQO1)和血红素氧合酶1(HO-1)m RNA表达。结果与正常对照组相比,缺氧组细胞内ROS含量明显增高(P<0.05),细胞活性明显降低(P<0.05);而缺氧+姜黄素预处理组缺氧导致的细胞氧化应激损伤明显减轻,并且细胞内的Nrf2蛋白水平明显增加(P<0.05),NQO1和HO-1 m RNA表达明显提高(P<0.05)。结论姜黄素能够激活视网膜神经节细胞内Nrf2抗氧化通路,上调其介导的Ⅱ相解毒酶和抗氧化酶基因NQO1和HO-1的转录活性,从而减轻缺氧导致的视网膜神经节细胞氧化应激损伤。展开更多
目的 :研究姜黄素对脓毒症小鼠急性肺损伤(acute Lung injury,ALI)的保护作用,探讨其作用机制。方法:动物随机分为手术组、假手术组、姜黄素组、卡托普利组和正常对照组,盲肠结扎穿孔术(cecal ligation and puncture,CLP)建立动物模型,...目的 :研究姜黄素对脓毒症小鼠急性肺损伤(acute Lung injury,ALI)的保护作用,探讨其作用机制。方法:动物随机分为手术组、假手术组、姜黄素组、卡托普利组和正常对照组,盲肠结扎穿孔术(cecal ligation and puncture,CLP)建立动物模型,利用光镜观察肺部组织形态学变化,称重法计算湿/干比(W/D),ELISA法检测血清中细胞因子TNF-α、IL-1β、IL-6水平,RIA法测定肺组织和血中AngⅡ的量。结果:与手术组比较,卡托普利(ACE拮抗剂)和姜黄素均可以使ALI/ARDS小鼠的氧合指数(259.5±24.2、268.8±21.5 vs 194.3±23.9)和W/D(5.35±0.25、5.13±0.59 vs 6.08±0.64)得到改善,都能不同程度地抑制体内AngⅡ的生成(肺:1.58±0.16、1.65±0.21 vs 2.38±0.41;血:178.04±17.87、153.74±10.24 vs 213.38±25.44),降低炎症因子水平,减轻肺组织病理学损伤。结论:姜黄素对脓毒症ALI具有保护作用,至少通过抑制炎症和抑制RAS两个重要机制发挥肺保护作用。展开更多
DSSCs （dye sensitized solar cells） are expected to be one of the efficient third generation solar cells that are generating green energy at low production cost since no vacuum systems or expensive equipment are requ...DSSCs （dye sensitized solar cells） are expected to be one of the efficient third generation solar cells that are generating green energy at low production cost since no vacuum systems or expensive equipment are required in their fabrication. In this paper, review of the structure, and operation principles of the dye sensitized solar cell DSSC is outlined. Extraction and optical characterization of Curcumin dye are detailed. Preparation procedures, optical and electrical characterization of the DSSC using Curcumin dye, as well as factors limiting the operation of the DSSC are discussed. Open circuit voltage Voc = 430 mV and short circuit current Ise = 0.72 mA from a 3 cm2 curcumin sensitized solar cell is achievable under solar illumination.展开更多
Aim To investigate the effect of curcumin on induction of glutathione S-transferases (GST), NADP(H):quinone oxidoreductase (NQO) and explore their possible molecular mechanism. Methods The activity of GST, NQO and cel...Aim To investigate the effect of curcumin on induction of glutathione S-transferases (GST), NADP(H):quinone oxidoreductase (NQO) and explore their possible molecular mechanism. Methods The activity of GST, NQO and cellular reduced glutathione (GSH) content were measured by spectrophotometrical methods. Cellular changes in the distribution of NF-E2 related factor 2 (Nrf2) was detected by western blotting analysis. Nrf2-AREs (antioxidant-responsive elements) binding activity was examined by electrophoretic mobility shift assay (EMSA). Results Curcumin caused a significant increase in GST and NQO activity, with a maximal value being observed at 20, 25 μmol/L, respectively. At concentration higher than 30 μmol/L, curcumin appeared to be less effective in stimulating GST and NQO activity. Treatment of HT-29 with curcumin caused a modest increase in the cellular GSH content at 3 h (P<0.05). All concentrations of curcumin continued to increase GSH content during the next 9 h. No significant change in the GSH content was observed at either 1 or 2 h after curcumin exposure. Upon treatment of cells with 20 μmol/L curcumin, Nrf2 protein accumulated rapidly (3 h) in the nucleus. This data suggested that curcumin mediate an increase of Nrf2 in the nuclear, a common component of ARE binding complexes. The retarded Nrf2-AREs complexes progressively formed upon treatment with curcumin at the concentration of 20 μmol/L for indicated time. Conclusion These results demonstrated that induction of GST and NQO activity by curcumin may be mediated by translocation of transcription factor Nrf2 from cytoplasm to nuclear and increased binding activity of Nrf2-ARE complexes.It is possible that this effect efficiently protects cells from oxidative stress and should be evaluated as a new therapeutic approach in oxidative stress-mediated damage.展开更多
文摘目的观察姜黄素对缺氧致小鼠视网膜神经节细胞RGC-5氧化应激损伤的影响,并探讨其作用机制。方法以小鼠RGC-5细胞株为研究对象,按照不同处理因素将细胞随机分为4组:正常对照组、缺氧组、缺氧+溶剂(二甲基亚砜)对照组、缺氧+姜黄素预处理组。2′7′-二氯荧光素探针法检测细胞内活性氧自由基(ROS),四甲基偶氮唑蓝法检测细胞增殖活性,Western blot检测细胞内核转录因子Nrf2蛋白的表达,实时荧光定量RT-PCR检测醌氧化还原酶(NQO1)和血红素氧合酶1(HO-1)m RNA表达。结果与正常对照组相比,缺氧组细胞内ROS含量明显增高(P<0.05),细胞活性明显降低(P<0.05);而缺氧+姜黄素预处理组缺氧导致的细胞氧化应激损伤明显减轻,并且细胞内的Nrf2蛋白水平明显增加(P<0.05),NQO1和HO-1 m RNA表达明显提高(P<0.05)。结论姜黄素能够激活视网膜神经节细胞内Nrf2抗氧化通路,上调其介导的Ⅱ相解毒酶和抗氧化酶基因NQO1和HO-1的转录活性,从而减轻缺氧导致的视网膜神经节细胞氧化应激损伤。
文摘目的 :研究姜黄素对脓毒症小鼠急性肺损伤(acute Lung injury,ALI)的保护作用,探讨其作用机制。方法:动物随机分为手术组、假手术组、姜黄素组、卡托普利组和正常对照组,盲肠结扎穿孔术(cecal ligation and puncture,CLP)建立动物模型,利用光镜观察肺部组织形态学变化,称重法计算湿/干比(W/D),ELISA法检测血清中细胞因子TNF-α、IL-1β、IL-6水平,RIA法测定肺组织和血中AngⅡ的量。结果:与手术组比较,卡托普利(ACE拮抗剂)和姜黄素均可以使ALI/ARDS小鼠的氧合指数(259.5±24.2、268.8±21.5 vs 194.3±23.9)和W/D(5.35±0.25、5.13±0.59 vs 6.08±0.64)得到改善,都能不同程度地抑制体内AngⅡ的生成(肺:1.58±0.16、1.65±0.21 vs 2.38±0.41;血:178.04±17.87、153.74±10.24 vs 213.38±25.44),降低炎症因子水平,减轻肺组织病理学损伤。结论:姜黄素对脓毒症ALI具有保护作用,至少通过抑制炎症和抑制RAS两个重要机制发挥肺保护作用。
文摘DSSCs （dye sensitized solar cells） are expected to be one of the efficient third generation solar cells that are generating green energy at low production cost since no vacuum systems or expensive equipment are required in their fabrication. In this paper, review of the structure, and operation principles of the dye sensitized solar cell DSSC is outlined. Extraction and optical characterization of Curcumin dye are detailed. Preparation procedures, optical and electrical characterization of the DSSC using Curcumin dye, as well as factors limiting the operation of the DSSC are discussed. Open circuit voltage Voc = 430 mV and short circuit current Ise = 0.72 mA from a 3 cm2 curcumin sensitized solar cell is achievable under solar illumination.
文摘Aim To investigate the effect of curcumin on induction of glutathione S-transferases (GST), NADP(H):quinone oxidoreductase (NQO) and explore their possible molecular mechanism. Methods The activity of GST, NQO and cellular reduced glutathione (GSH) content were measured by spectrophotometrical methods. Cellular changes in the distribution of NF-E2 related factor 2 (Nrf2) was detected by western blotting analysis. Nrf2-AREs (antioxidant-responsive elements) binding activity was examined by electrophoretic mobility shift assay (EMSA). Results Curcumin caused a significant increase in GST and NQO activity, with a maximal value being observed at 20, 25 μmol/L, respectively. At concentration higher than 30 μmol/L, curcumin appeared to be less effective in stimulating GST and NQO activity. Treatment of HT-29 with curcumin caused a modest increase in the cellular GSH content at 3 h (P<0.05). All concentrations of curcumin continued to increase GSH content during the next 9 h. No significant change in the GSH content was observed at either 1 or 2 h after curcumin exposure. Upon treatment of cells with 20 μmol/L curcumin, Nrf2 protein accumulated rapidly (3 h) in the nucleus. This data suggested that curcumin mediate an increase of Nrf2 in the nuclear, a common component of ARE binding complexes. The retarded Nrf2-AREs complexes progressively formed upon treatment with curcumin at the concentration of 20 μmol/L for indicated time. Conclusion These results demonstrated that induction of GST and NQO activity by curcumin may be mediated by translocation of transcription factor Nrf2 from cytoplasm to nuclear and increased binding activity of Nrf2-ARE complexes.It is possible that this effect efficiently protects cells from oxidative stress and should be evaluated as a new therapeutic approach in oxidative stress-mediated damage.
