A Study on the Assessment and Integration of Multi-Source Evapotranspiration Products over the Tibetan Plateau

Evapotranspiration(ET)is a crucial variable in the terrestrial water,carbon,and energy cycles.At present,a large number of multi source ET products exist.Due to sparse observations,however,great challenges exist in the evaluation and integration of ET products in remote and complex areas such as the Tibetan Plateau(TP).In this paper,the applicability of the multiple collocation(MC)method over the TP is evaluated for the first time,and the uncertainty of multisource ET products(based on reanalysis,remote sensing,and land surface models)is further analyzed,which provides a theoretical basis for ET data fusion.The results show that 1)ET uncertainties quantified via the MC method are lower in RS-based ET products(5.95 vs.7.06 mm month^(-1))than in LSM ET products(10.22 vs.17.97 mm month^(-1))and reanalysis ET estimates(7.27 vs.12.26 mm month-1).2)A multisource evapotranspiration(MET)dataset is generated at a monthly temporal scale with a spatial resolution of 0.25°across the TP during 2005-15.MET has better performance than any individual product.3)Based on the fusion product,the total ET amount over the TP and its patterns of spatiotemporal variability are clearly identified.The annual total ET over the entire TP is approximately 380.60 mm.Additionally,an increasing trend of 1.59±0.85 mm yr^(-1)over the TP is shown during 2005-15.This study provides a basis for future studies on water and energy cycles and water resource management over the TP and surrounding regions.

Unveiling evapotranspiration patterns and energy balance in a subalpine forest of the Qinghai-Tibet Plateau:observations and analysis from an eddy covariance system

Evapotranspiration is an important parameter used to characterize the water cycle of ecosystems.To under-stand the properties of the evapotranspiration and energy balance of a subalpine forest in the southeastern Qinghai-Tibet Plateau,an open-path eddy covariance system was set up to monitor the forest from November 2020 to October 2021 in a core area of the Three Parallel Rivers in the Qing-hai-Tibet Plateau.The results show that the evapotranspira-tion peaked daily,the maximum occurring between 11:00 and 15:00.Environmental factors had significant effects on evapotranspiration,among them,net radiation the greatest(R^(2)=0.487),and relative humidity the least(R^(2)=0.001).The energy flux varied considerably in different seasons and sensible heat flux accounted for the main part of turbulent energy.The energy balance ratio in the dormant season was less than that in the growing season,and there is an energy imbalance at the site on an annual time scale.

SimET: An open-source tool for estimating crop evapotranspiration and soil water balance for plants with multiple growth cycles

Accurate estimation of crop evapotranspiration(ETc) and soil water balance, which is vital for optimizing water management strategy in crop production, can be performed by simulation. But existing software has many deficiencies, including complex operation, limited scalability, lack of batch processing, and a single ETc model. Here we present simET, an open-source software package written in the R programming language. Many concepts involved in crop ETc simulation are condensed into functions in the package. It includes three widely used crop ETc models built on these functions: the single-crop coefficient,double-crop coefficient, and Shuttleworth–Wallace models, along with tools for preparing model data and comparing estimates. SimET supports ETc simulation in crops with repeated growth cycles such as alfalfa, a perennial forage crop that is cut multiple times annually.

Evaluation of Actual Evapotranspiration and Crop Coefficient in Carrot by Remote Sensing Methodology Using Drainage and River Water to Overcome Reduced Water Availability

Searching for alternative methods for traditional irrigation is World trend at days due to a reduction in water and increased of drought due to climate changes therefore farmers need use modern methods of scheduling water and minimizing water losses while also increasing yield. To meet the future increasing demands water and food there is a need to utilize alternative methods to reduce evaporation, transpiration and deep percolation of water. Any countries use recycled water (drain and sewage) and desalination water from the sea or drains to irrigate crops plus computing actual crop evapotranspiration (ETc) so as to calculate the amount of water to apply to a crop. The paper aims to assess the actual evaporation and evaporation coefficient of carrots, by planting carrots in a field and the crop was exposed to several sources of water (DW and RW) and comparing ETc, Kc and production among plots of three sites (A, B and C). The study used two types of irrigation water (drain water (DW) and river water (RW)). The results were to monthly rate and accumulated actual evapotranspiration to C (irrigation by RW only) more than A (67% RW and 33% DW) and B (17% RW and 83% DW) via 7% and 58%, respectively. The yield to C more than A and B by 17% and 75%, respectively. In conclusion the use of DW can cause a reduction in crop consumptive of carrot crops also causes a reduction in yield, crop length, root length, root size, canopy of crop, number of leaves and biomass of the plant therefore, the drainage water needs to treated before irrigating crops And making use of it to irrigate the fields and fill the shortfall in the amount of water from the river. The drain water helped on filling the water shortage due to climate changes and giving production of carrot crop but less than river water.

Estimation of the Actual Evapotranspiration by the SEBAL Method in the Irrigated Rice Perimeter of Zatta (Yamoussoukro—Côte d’Ivoire)

In this study, the SEBAL (Surface Energy Balance Algorithm for Land) model was used to map the spatio-temporal distribution of actual evapotranspiration in the Yamoussoukro department (Côte d’Ivoire). Like other regions of the country, the Yamoussoukro district is confronted with the phenomenon of evapotranspiration (ET). This is a very important component that comes into play in the water balance but also in the calculation of the water needs of agricultural crops. Consequently, its estimation is of paramount importance in research related to the rational management of water resources, particularly agricultural water. The objective of this study was to analyze the spatio-temporal distribution of actual evapotranspiration (AET) as a function of land cover and land use. The methodology used is based on the SEBAL model which uses remote sensing (Landsat 8_OLI/TIRS) and climatic data to estimate actual evapotranspiration and analyze the spatio-temporal distribution of AET. The results reveal that the AET varied from 0 to 5.44 mm/day over the period from December 2019 to February 2020 with an average value of 4.92 mm/day. The highest average values occurred for water bodies (4.90 mm/day) and flooded vegetation (4.88 mm/day) while the lowest values occurred in residential areas (2.04 mm/day). Furthermore, the results show that the difference between the SEBAL model and the FAO-Penman-Monteith method is minimal with an average RMSE of 0.36 mm/day for all the satellite images. This study demonstrates the considerable potential of remote sensing for the characterization and estimation of spatial evapotranspiration in the Zatta irrigated rice-growing area.

Responses of vegetation yield to precipitation and reference evapotranspiration in a desert steppe in Inner Mongolia,China

Drought,which restricts the sustainable development of agriculture,ecological health,and social economy,is affected by a variety of factors.It is widely accepted that a single variable cannot fully reflect the characteristics of drought events.Studying precipitation,reference evapotranspiration(ET_(0)),and vegetation yield can derive information to help conserve water resources in grassland ecosystems in arid and semi-arid regions.In this study,the interactions of precipitation,ET_(0),and vegetation yield in Darhan Muminggan Joint Banner(DMJB),a desert steppe in Inner Mongolia Autonomous Region,China were explored using two-dimensional(2D)and three-dimensional(3D)joint distribution models.Three types of Copula functions were applied to quantitatively analyze the joint distribution probability of different combinations of precipitation,ET_(0),and vegetation yield.For the precipitation–ET_(0)dry–wet type,the 2D joint distribution probability with precipitation≤245.69 mm/a or ET_(0)≥959.20 mm/a in DMJB was approximately 0.60,while the joint distribution probability with precipitation≤245.69 mm/a and ET_(0)≥959.20 mm/a was approximately 0.20.Correspondingly,the joint return period that at least one of the two events(precipitation was dry or ET_(0)was wet)occurred was 2 a,and the co-occurrence return period that both events(precipitation was dry and ET_(0)was wet)occurred was 5 a.Under this condition,the interval between dry and wet events would be short,the water supply and demand were unbalanced,and the water demand of vegetation would not be met.In addition,when precipitation remained stable and ET_(0)increased,the 3D joint distribution probability that vegetation yield would decrease due to water shortage in the precipitation–ET_(0)dry–wet years could reach up to 0.60–0.70.In future work,irrigation activities and water allocation criteria need to be implemented to increase vegetation yield and the safety of water resources in the desert steppe of Inner Mongolia.

Correlation Analysis between the Evapotranspiration Quantity and Climatic Factors of Artificial Grassland in Three River Sources Areas

Based on observation data from the mini-type automatic weather station,the evapotranspiration quantity of reference crops from artificial grassland in three river sources areas was estimated by the method of FAO Penman-Monteith.The actual evapotranspiration quantity of grassland was calculated according to the synthetic crop coefficients referenced by FAQ-56,and the change of the actual evapotranspiration quantity of artificial grassland in three river sources areas as well as the relationship between the evapotranspiration quantity and climatic factors were studied.The results suggested that the seasonal change of actual evapotranspiration quantity in grassland was expressed in a single peak curve with the peak in the middle August,and daily transpiration quantity in summer was significantly larger than that in winter.The evapotranspiration was significantly correlated with air temperature,solar radiation and relative humidity,but not significantly correlated with wind speed.Effects of climatic factors on the evapotranspiration quantity of artificial grassland were ordered as follow:air temperature(T)>solar radiation(Ra)>relative humidity(RH)>wind speed(u2).

Estimation of Regional Evapotranspiration in Alpine Area and Its Response to Land Use Change:A Case Study in Three-River Headwaters Region of Qinghai-Tibet Plateau,China

Three-River Headwaters （TRH） region involved in this paper refers to the source region of the Changjiang （Yangtze） River, the Huanghe （Yellow） River and the Lancang River in China. Taking the TRH region of the Qing- hai-Tibet Plateau as a case, the annual evapotranspiration （ET） model developed by Zhang et al. （2001） was applied to evaluate mean annual ET in the alpine area, and the response of annual ET to land use change was analyzed. The plant-available water coefficient （w） of Zhang＇s model was revised by using vegetation-temperature condition index （VTCI） before annual ET was calculated in alpine area. The future land use scenario, an input of ET model, was spa- tially simulated by using the conversion of land use and its effects at small regional extent （CLUE-S） to study the re- sponse of ET to land use change. Results show that the relative errors between the simulated ET and that calculated by using water balance equation were 3.81% and the index of agreement was 0.69. This indicates that Zhang＇s ET model based on revised plant-available water coefficient is a scientific and practical tool to estimate the annual ET in the al- pine area. The annual ET in 2000 in the study area was 221.2 ram, 11.6 mm more than that in 1980. Average annual ET decreased from southeast to northwest, but the change of annual ET between 1980 and 2000 increased from southeast to northwest. As a vast and sparsely populated area, the population in the TRH region was extremely unbalanced and land use change was concentrated in very small regions. Thus, land use change had little effect on total annual ET in the study area but a great impact on its spatial distribution, and the effect of land use change on ET decreased with in- creasing precipitation. ET was most sensitive to the interconversion between forest and unused land, and was least sen- sitive to the interconversion between cropland and low-covered grassland.

Temporal and Spatial Distribution Characteristics of Potential Evapotranspiration and Its Sensitivity to Meteorological Factors in Guizhou Province

[Objective] The study aims to discuss the changes of potential evapotran- spiration and its sensitivity to meteorological factors in Guizhou Province, so as to provide important references for assessment of water resources, research of agri- cultural water conservancy and climate change. [Method] Temporal and spatial dis- tribution characteristics of potential evapotranspiration in Guizhou Province from 1961 to 2010 were analyzed, and the sensitivity of potential evapotranspiration to meteo- rological factors in Guizhou Province was studied through correlation analysis. [Re- sult] On the whole, potential evapotranspiration in Guizhou Province was higher in the southwest and the west compared with the northeast and the east. In various seasons, it was the highest in summer, followed by spring and autumn, while it was the lowest in winter. In recent 40 years, annual potential evapotranspiration showed an obvious decreasing trend in most stations of Guizhou Province. The main meteo- rological factors influencing changes of potential evapotranspiration in Guizhou Province were sunshine duration, daily maximum temperature, and daily average relative humidity. [Conclusion] Daily average temperature was not the main meteoro- logical factor affecting changes of potential evapotranspiration in Guizhou Province, while sunshine duration, daily maximum temperature, and daily average relative hu- midity had important effects on potential evapotranspiration in Guizhou Province.

转录因子ETS1激活长链非编码RNA XIST促进胶质瘤细胞增殖

目的探讨ETS原癌基因1(ETS1)在胶质瘤中的生物学功能及其下游机制。方法生物信息学和免疫组织化学分析ETS1在胶质瘤组织中的表达;实时定量PCR(qRT-PCR)检测ETS1 mRNA和长链非编码RNA(lncRNA)X染色体失活特异转录本(XIST)的表达水平。CCK-8和5-乙炔基-2'-脱氧尿苷摄入实验检测细胞增殖。Western blot检测凋亡相关蛋白(Bax、Bak、Bcl-2)的表达。PROMO数据库预测ETS1与XIST启动子的结合位点。双荧光素酶报告基因实验和染色质免疫共沉淀-PCR用于验证ETS1与XIST启动子区域的结合关系。cBioPortal数据库分析ETS1 mRNA与lncRNA XIST在胶质瘤组织中表达的相关性。结果ETS1 mRNA和蛋白的表达水平在胶质瘤中显著上调(P<0.05)。敲低ETS1可显著抑制胶质瘤细胞增殖(P<0.05)并促进细胞凋亡(P<0.05)。ETS1可靶向结合XIST并促进XIST的表达(P<0.05),过表达XIST可逆转敲低ETS1对胶质瘤细胞增殖的抑制作用(P<0.05)以及对细胞凋亡的促进作用(P<0.05)。结论ETS1在胶质瘤组织中高表达,其可能通过促进lncRNA XIST高表达而减少细胞凋亡和促进胶质瘤细胞增殖。

Study on Artificial Neural Network Model for Crop Evapotranspiration

Based on potted plant experiment, BP-artifieial neural network was used to simulate crop evapotranspiration and 3 kinds of artificial neural network models were constructed as ET1 （meteorological factors）, ET2（ meteorological factors and sowing days） and ET3 （meteorological factors, sowing days and water content）. And the predicted result was compared with actual value ET that was obtained by weighing method. The results showed that the ET3 model had higher calculation precision and an optimum BP-artificial neural network model for calculating crop evapotranspiration.

ET-1mRNA反义寡核苷酸治疗模式下糖尿病大鼠生存状况及肾脏病理进展研究

目的对糖尿病肾病(DN)大鼠模型利用内皮素反义寡核苷酸(ET-1AS-ODN)技术进行干预治疗,探讨ET-1AS-ODN在糖尿病肾病(DN)早期肾功能的保护作用。方法使用64只健康的SD雄性大鼠,通过腹腔注射链尿佐菌素(STZ)以剂量为55~60 mg/kg的方法制备糖尿病模型。随后,对其中32只大鼠给予ET-1AS-ODN 6 OD/kg/wk的治疗。在治疗过程中,对大鼠的生存状况及肾功能病理进展情况进行动态观察和测量。结果经过2、4、6、8周实验动物饲养后,与生理盐水对照组相比,8周后模型组(DM)生存率极低(P<0.05),肾功能检测血肌酐(Scr)、尿肌酐、尿素氮(BUN)水平、ET-1含量显著增高。ET-1AS-ODN治疗组在8周后效果显著,能够明显降低DN大鼠的血肌酐(Scr)和尿素氮(BUN)水平,并提高肌酐清除率(Ccr),其差异有统计学意义(P<0.05)。结论经AS-ODN早期干预治疗可以有效改善糖尿病大鼠的生存状态,并对肾功能具有保护作用。

Parametric analyses of evapotranspiration landfill covers in humid regions

Natural soils are more durable than almost all man-made materials. Evapotranspiration （ET） covers use vegetated soil layers to store water until it is either evaporated from the soil surface or transpired through vegetation. ETcovers rely on the water storage capacity of soil layer, rather than low permeability materials, to minimize percolation. While the use of ET covers in landfills increased over the last decade, they were mainly used in arid or semi-arid regions. At present, the use of ET covers has not been thoroughly investigated in humid areas. The purpose of this paper is to investigate the use of ETcovers in humid areas where there is an annual precipitation of more than 800 mm. Numerical analyses were carried out to investigate the influences of cover thickness, soil type, vegetation level and distribution of precipitation on performance of ET covers. Performance and applicability of capillary barriers and a new-type cover were analyzed. The results show that percolation decreases with an increasing cover thickness and an increasing vegetation level, but the increasing trend becomes unclear when certain thickness or LAI （leaf area index） is reached. Cover soil with a large capability of water storage is recommended to minimize percolation. ET covers are significantly influenced by distribution of precipitation and are more effective in areas where rainy season coincides with hot season. Capillary barriers are more efficient than monolithic covers. The new cover is better than the monolithic cover in performance and the final percolation is only 0.5% of the annual precipitation.

子宫内膜不典型增生/早期子宫内膜癌患者保留生育功能治疗后IVF-ET妊娠结局及复发因素分析

目的分析子宫内膜不典型增生/早期子宫内膜癌(AH/EEC)患者保留生育功能治疗后接受体外受精-胚胎移植(IVF-ET)治疗的临床特点和预后,分析影响助孕妊娠结局和疾病复发的主要因素。方法回顾性分析2012年2月至2022年2月在北京协和医院接受AH/EEC生育保留治疗后进行IVF-ET治疗的78例患者的临床资料。总结分析纳入患者的临床特征、IVF-ET相关指标、妊娠结局和复发情况,以单因素和多因素分析临床妊娠率、活产率以及疾病复发的影响因素。结果78例患者中51例(65.38%)为AH患者,27例(34.62%)为EEC患者;开始IVF-ET周期的平均年龄为(34.17±3.70)岁。共有74例患者至少接受了1次移植,每移植周期的临床妊娠率和活产率分别为36.31%(65/179)和18.99%(34/179),累积妊娠率为72.97%(54/74)。多因素分析提示子宫内膜病变初次发病年龄是活产率的独立影响因素[OR=0.8794,95%CI(0.785,0.983),P=0.02]。纳入患者IVF-ET期间子宫内膜病变的总复发率为6.41%(5/78),多因素分析提示子宫内膜病变的病理类型和IVF-ET前复发史是疾病复发的危险因素(P<0.05)。结论AH/EEC患者保留生育功能治疗后的辅助生殖结局相对满意,在肿瘤治疗过程中,进行病变评估时应尽量保护内膜,减少损伤;在肿瘤治疗结束后,应尽快进行助孕治疗,以最大程度降低复发率。

眼络通方对大鼠视网膜静脉阻塞模型ET-1/ETAR信号通路的影响

目的探讨眼络通对大鼠视网膜静脉阻塞(RVO)模型ET-1/ETAR信号通路的影响。方法将48只健康雄性SD大鼠随机分为空白对照组(Control),模型对照组(Model)、波生坦组(Bosentan)、眼络通组(Yanluotong),以光化学法建立RVO模型SD大鼠各12只,连续给药3周。在第3周时处死SD大鼠,取血清用于Elisa检测,取视网膜组织用于荧光定量PCR与免疫荧光检测,测定其中ET-1、ETAR、VEGF、TNF-α、IL-6蛋白与mRNA表达水平。结果Elisa检测发现模型对照组血清IL-6、TNF-α、VEGF较空白对照组升高(P<0.01),波生坦和眼络通方干预3周后比模型对照组均下降(P<0.01);免疫荧光显示相对于空白对照组,RVO模型对照组ET-1、IL-6、TNF-α、VEGF表达量均增多,波生坦和眼络通方治疗后表达量均呈下降趋势。对于ET-1、ETAR、VEGF mRNA表达量,模型对照组较空白对照组上调(P<0.01),波生坦和眼络通方均能下调上述基因mRNA的表达(P<0.01)。结论ET-1/ETAR信号通路在RVO发病过程中被异常激活,眼络通方可通过ET-1/ETAR信号通路发挥抑制视网膜血管收缩、减轻炎症反应、抑制VEGF作用,干预RVO病情进展。

精液优化处理后DNA碎片指数与IVF-ET胚胎质量及妊娠结局的关系

目的:评估经密度梯度离心联合上游法优化处理后的精子DNA碎片指数(DFI)与辅助生殖体外受精-胚胎移植(IVF-ET)胚胎质量及妊娠结局的关系。方法:回顾性分析2022年4月至12月在郑州大学第一附属医院生殖医学中心因单纯输卵管因素行IVF-ET的257个周期,比较男方精液优化前后精液参数和精子DFI;按优化后精子DFI将其分为高DFI组(DFI>5)与低DFI组(DFI≤5),比较两组的胚胎发育及妊娠结局;根据临床妊娠情况分为妊娠组与非妊娠组、持续妊娠组及早期流产组,比较精子DFI情况。结果:与处理前相比,精液优化处理后前向运动精子及正常形态精子百分比提高,精子DFI下降(P<0.001)。精子优化处理后高DFI组的早期流产率高于低DFI组(P<0.05)。105例临床妊娠周期中早期流产组精液优化处理前、后DFI均高于持续妊娠组(P<0.05)。结论:密度梯度离心联合上游优化处理是一种有效的精液制备方法,可提高精子前向运动能力和正常形态精子比例,降低精子DFI;优化后精子高DFI可能增加临床妊娠后早期流产的风险。

Evapotranspiration and Its Energy Exchange in Alpine Meadow Ecosystem on the Qinghai-Tibetan Plateau

To understand the water and energy exchange on the Qinghai-Tibetan Plateau, we explored the characteristics of evapotranspiration （ET） and energy fluxes from 2002 to 2005 over a Kobresia meadow ecosystem using the eddy covariance method. The ratio of annual ET to precipitation （P） of meadow ecosystem was about 60%, but varied greatly with the change of season from summer to winter. The annual ET/P in meadow was lower than that in shrub, steppe and wetland ecosystems of this plateau. The incident solar radiation （Rs） received by the meadow was obviously higher than that of lowland in the same latitude; however the ratio of net radiation （Rn） to Rs with average annual value of 0.44 was significantly lower than that in the same latitude. The average annual ET was about 390 mm for 2002-2005, of which more than 80% occurred in growing season from May to September. The energy consumed on the ET was about 44% of net radiation in growing season, which was lower than that of shrub, steppe and wetland on this plateau. This study demonstrates that the Kobresia meadow may prevent the excessive water loss through evapotranspiration from the ecosystem into the atmosphere in comparison to the shrub, steppe and wetland ecosystems of the Qinghai-Tibetan Plateau.

Effects of Evapotranspiration on Mitigation of Urban Temperature by Vegetation and Urban Agriculture

The temperature difference between an urban space and surrounding non-urban space is called the urban heat island effect （UHI）. Global terrestrial evapotranspiration （ET） can consume 1.4803x1023joules （J） of energy annually, which is about 21.74% of the total available solar energy at the top of atmosphere, whereas annual human energy use is 4.935× 1020 J, about 0.33% of annual ET energy consumption. Vegetation ET has great potential to reduce urban and global temperatures. Our literature review suggests that vegetation and urban agricultural ET can reduce urban temperatures by 0.5 to 4.0℃. Green roofs （including urban agriculture） and water bodies have also been shown to be effective ways of reducing urban temperatures. The cooling effects on the ambient temperature and the roof surface temperature can be 0.24-4.0℃ and 0.8-60.0℃, respectively. The temperature of a water body （including urban aquaculture） can be lower than the temperature of the surrounding built environment by between 2 and 6℃, and a water body with a 16 m2 surface area can cool up to 2 826 m3 of nearby space by 1℃. Based on these findings, it can be concluded that the increase of evapotranspiration in cities, derived from vegetation, urban agriculture, and water body, can effectively mitigate the effect of urban heat islands.

How Does the Partitioning of Evapotranspiration and Runoff between Different Processes Affect the Variability and Predictability of Soil Moisture and Precipitation?

Water stored as part of the land surface is lost to evapotranspiration and runoff on different time scales, and the partitioning between these time scales is important for modeling soil water in a climate model. Different time scales are imposed on evapotranspiration primarily because it is derived from different reservoirs with different storage capacities, from the very rapid evaporation of canopy stores to the slow removal by transpiration of rooting zone soil moisture. Runoff likewise ranges in time scale from rapid surface terms to the slower base-flow. The longest time scale losses of water determine the slow variation of soil moisture and hence the longer time scale effects of soil moisture on precipitation. This paper shows with a simple analysis how shifting the partitioning of evapotranspiration between the different reservoirs affects the variability of soil moisture and precipitation. In particular, it is concluded that a shift to shorter time scale reservoirs shifts the variance of precipitation from that which is potentially predictable to unpredictable.

Trends in major and minor meteorological variables and their influence on reference evapotranspiration for mid Himalayan region at east Sikkim, India

Abstract： Estimation of evapotranspiration （ET） for mountain ecosystem is of absolute importance since it serves as an important component in balancing the hydrologic cycle. The present study evaluates the performance of original and location specific calibrated Hargreaves equation （HARG） with the estimates of Food and Agricultural Organization （FAO） Penman Monteith （PM） method for higher altitudes in East Sikkim, India. The results show that the uncalibrated HARG model underestimates ET0 by 0.35 mm day^-1 whereas the results are significantly improved by regional calibration of the model. In addition, this paper also presents the variability in the trajectory associated with the climatic variables with the changing climate in the study site. Non- parametric Mann-Kendall （MK） test was used to investigate and understand the mean monthly trend of eight climatic parameters including reference evapotranspiration （ET0） for the period of 1985 - 2009. Trend of ET0 was estimated for the calculations done by FAO PM equation. The outcomes of the trend analysis show significant increasing （p ≤ 0.05） trend represented by higher Z-values, through MK test, for net radiation （Rn）, maximum temperature （Tmax） and minimum temperature （Train）, especially in the first months of the year. Whereas, significant （0.01 ≥ p ≤0.05） decreasing trend in vapor pressure deficit （VPD） and precipitation （P） is observed throughout the year. Declining trend in sunshine duration, VPD and ET0 is found in spring （March - May） and monsoon （June - November） season. The result displays significant （0.01≤ p ≤0.05） decreasing ET0 trend between （June - December） except in July, exhibiting the positive relation with VPD followed by sunshine duration at the station. Overall, the study emphasizes the importance of trend analysis of ET0 and other climatic variables for efficient planning and managing the agricultural practices, in identifying the changes in the meteorological parameters and to accurately assess the hydrologic water balance of the hilly regions.