In this study, the event-specific real-time fluorescence quantitative PCR method established by Siehuan Academy of Agricultural Sciences was employed to detect the content of flanking fragment （specific fragment） in...In this study, the event-specific real-time fluorescence quantitative PCR method established by Siehuan Academy of Agricultural Sciences was employed to detect the content of flanking fragment （specific fragment） in samples containing 2% genetically modified maize event NK603. The uncertainty of detection results was evaluated based on various uncertainty sources, such as PCR amplification system, data analysis and micropipette. The results showed that A-type uncertainty （ uA ）, B-type uncertainty （ uB ）, combined standard uncertainty （ uC ） and expanded uncertainty （ U95 ） were 0. 000 8,0.1301,0. 001 and 0. 002, respectively; the final detection result was 1.9% ±0.002. Thus, the main uncertainty in detecting flanking fragment of genetically modified maize event NK603 with realtime fluorescence quantitative PCR method was derived from the random effect in the experimental process.展开更多
Geologic marks related to extraterrestrial impact events, such as impact split gravels, impact brecciate layers, impact dikes, microirghizites, microtektites, especially meteoritic residues, were discovered on the bou...Geologic marks related to extraterrestrial impact events, such as impact split gravels, impact brecciate layers, impact dikes, microirghizites, microtektites, especially meteoritic residues, were discovered on the boundary between Sinian/Cambrian at Tianmenshan of Western Hunan, which may possibly demonstrate that an extraterrestrial impact event has ever occurred there on the S/C boundary.展开更多
基金Supported by Youth Fund of Sichuan Academy of Agricultural Sciences(2009QNJJ-037)
文摘In this study, the event-specific real-time fluorescence quantitative PCR method established by Siehuan Academy of Agricultural Sciences was employed to detect the content of flanking fragment （specific fragment） in samples containing 2% genetically modified maize event NK603. The uncertainty of detection results was evaluated based on various uncertainty sources, such as PCR amplification system, data analysis and micropipette. The results showed that A-type uncertainty （ uA ）, B-type uncertainty （ uB ）, combined standard uncertainty （ uC ） and expanded uncertainty （ U95 ） were 0. 000 8,0.1301,0. 001 and 0. 002, respectively; the final detection result was 1.9% ±0.002. Thus, the main uncertainty in detecting flanking fragment of genetically modified maize event NK603 with realtime fluorescence quantitative PCR method was derived from the random effect in the experimental process.
基金financially supported by China National Natural Science Foundation(Grant No.40172078).
文摘Geologic marks related to extraterrestrial impact events, such as impact split gravels, impact brecciate layers, impact dikes, microirghizites, microtektites, especially meteoritic residues, were discovered on the boundary between Sinian/Cambrian at Tianmenshan of Western Hunan, which may possibly demonstrate that an extraterrestrial impact event has ever occurred there on the S/C boundary.
