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Microstructure Characteristics and Possible Phase Evolution of the Coal Gangue-Steel Slag Ceramics Prepared by the Solid-State Reaction Methods
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作者 刘文洁 WANG Yang +1 位作者 LI Jingtao 李宝让 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2024年第4期921-930,共10页
Industrial wastes such as steel slag and coal gangue etc.were chosen as raw materials for preparing ceramic via the conventional solid-state reaction method.With steel slag and coal gangue mixed in various mass ratios... Industrial wastes such as steel slag and coal gangue etc.were chosen as raw materials for preparing ceramic via the conventional solid-state reaction method.With steel slag and coal gangue mixed in various mass ratios,from 100%steel slag to 100%coal gangue at 10%intervals,microstructure and possible phase evolution of the coal gangue-steel slag ceramics were investigated using X-ray powder diffraction,scanning electron microscopy,mercury intrusion porosimetry and Archimedes boiling method.The experimental results suggest that the phase compositions of the as-prepared ceramics could be altered with the increased amount of coal gangue in the ceramics.The anorthite-diopside eutectic can be formed in the ceramics with the mass ratios of steel slag to coal gangue arranged from 8:2 to 2:8,which was responsible for the melting of the steel slag-coal gangue ceramics at relatively high temperature.Further investigations on the microstructure suggested that the addition of the proper amount of steel slag in ceramic compositions was conducive to the pore formation and further contributed to an increment in porosity. 展开更多
关键词 steel slag-coal gangue ceramics SYNTHESIS phase evolution microstructure characteristics
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Applications of single-cell RNA sequencing in spermatogenesis and molecular evolution
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作者 Wen-Bo Chen Meng-Fei Zhang +1 位作者 Fan Yang Jin-Lian Hua 《Zoological Research》 SCIE CSCD 2024年第3期575-585,共11页
Spermatogenic cell heterogeneity is determined by the complex process of spermatogenesis differentiation.However,effectively revealing the regulatory mechanisms underlying mammalian spermatogenic cell development and ... Spermatogenic cell heterogeneity is determined by the complex process of spermatogenesis differentiation.However,effectively revealing the regulatory mechanisms underlying mammalian spermatogenic cell development and differentiation via traditional methods is difficult.Advances in technology have led to the emergence of many single-cell transcriptome sequencing protocols,which have partially addressed these challenges.In this review,we detail the principles of 10x Genomics technology and summarize the methods for downstream analysis of single-cell transcriptome sequencing data.Furthermore,we explore the role of single-cell transcriptome sequencing in revealing the heterogeneity of testicular ecological niche cells,delineating the establishment and disruption of testicular immune homeostasis during human spermatogenesis,investigating abnormal spermatogenesis in humans,and,ultimately,elucidating the molecular evolution of mammalian spermatogenesis. 展开更多
关键词 Single-cell RNA sequencing(scRNA-seq) SPERMATOGENESIS molecular evolution Sertoli cell
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Characteristic stress variation and microcrack evolution of granite subjected to uniaxial compression using acoustic emission methods
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作者 Yunge Zhao Xibing Li +2 位作者 Linqi Huang Zhichao Zhang Zihou Xu 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第9期3511-3523,共13页
The size of mineral grain has a significant impact on the initiation and propagation of microcracks within rocks.In this study,fine-,medium-,and coarse-grained granites were used to investigate microcrack evolution an... The size of mineral grain has a significant impact on the initiation and propagation of microcracks within rocks.In this study,fine-,medium-,and coarse-grained granites were used to investigate microcrack evolution and characteristic stress under uniaxial compression using the acoustic emission(AE),digital image correlation(DIC),and nuclear magnetic resonance(NMR)measurements.The experimental results show that the characteristic stress of each granite decreased considerably with increasing grain sizes.The inflection points of the b-value occurred earlier with an increase in grain sizes,indicating that the larger grains promote the generation and propagation of microcracks.The distribution characteristics of the average frequency(AF)and the ratio of rise time to amplitude(RA)indicate that the proportion of shear microcracks increases with increasing grain size.The NMR results indicate that the porosity and the proportion of large pores increased with increasing grain size,which may intensify the microcrack evolution.Moreover,analysis of the DIC and AE event rates suggests that the high-displacement regions could serve as a criterion for the degree of microcrack propagation.The study found that granites with larger grains had a higher proportion of high-displacement regions,which can lead to larger-scale cracking or even spalling.These findings are not only beneficial to understand the pattern of microcrack evolution with different grain sizes,but also provide guidance for rock monitoring and instability assessment. 展开更多
关键词 Grain size Acoustic emission(AE) characteristic stress Microcrack evolution Fracture mode
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Structural Characteristics and Evolution of a Weighted Sino-US Container Shipping Network
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作者 ZHANG Tiantian XI Daping +3 位作者 JIANG Wenping FENG Yuhao WANG Chuyuan HU Xini 《Chinese Geographical Science》 SCIE CSCD 2024年第5期810-828,共19页
This study selected the Sino-US route data from the top 30 global container liner companies between December 1,2019,and December 29,2019,as the data source utilizing the complex network research methodology.It constru... This study selected the Sino-US route data from the top 30 global container liner companies between December 1,2019,and December 29,2019,as the data source utilizing the complex network research methodology.It constructs a Sino-US container shipping network through voyage weighting and analyzes the essential structural characteristics to explore the network’s complex structural fea-tures.The network’s evolution is examined from three perspectives,namely,time,space,and event influence,aiming to comprehens-ively explore the network’s evolution mechanism.The results revealed that:1)the weighted Sino-US container shipping network exhib-its small-world and scale-free properties.Key hub ports in the United States include NEW YORK NY,SAVANNAH GA,LOS ANGELES CA,and OAKLAND CA,whereas SHANGHAI serving as the hub port in China.The geographical distribution of these hub ports is uneven.2)Concerning the evolution of the weighted Sino-US container shipping network,from a temporal perspective,the evolution of the regional structure of the entire Sino-US region and the Inland United States is in a stage of radiative expansion and de-velopment,with a need for further enhancement in competitiveness and development speed.The evolution of the regional structure of southern China and Europe is transitioning from the stage of radiative expansion and development to an advanced equilibrium stage.The shipping development in Northern China,the Western and Eastern United States,and Asia is undergoing significant changes but faces challenges of fierce competition and imbalances.From a spatial perspective,the rationality and effectiveness of the improved weighted Barrat-Barthelemy-Vespignani(BBV)model are confirmed through theoretical derivation.The applicability of the improved evolution model is verified by simulating the evolution of the weighted Sino-US container shipping network.From an event impact per-spective,the Corona Virus Disease 2019(COVID-19)pandemic has not fundamentally affected the spatial pattern of the weighted Sino-US container shipping network but has significantly impacted the network’s connectivity.The network lacks sufficient resilience and stability in emergency situations.3)Based on the analysis of the structural characteristics and evolution of the weighted Sino-US con-tainer shipping network,recommendations for network development are proposed from three aspects:emphasizing the development of hub ports,focusing on the balanced development of the network,and optimizing the layout of Chinese ports. 展开更多
关键词 container shipping network structure characteristics network evolution voyage weighting improved Barrat-Barthelemy-Vespignani(BBV)model
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Evolution and Characteristics of "Carbon Neutrality Movies"
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作者 Licheng LIU Di ZHANG 《Meteorological and Environmental Research》 2024年第3期45-48,共4页
"Carbon neutrality movies"are movies that focus on carbon neutrality as the object of expression and dissemination.Using carbon neutrality as an element,it influences the development of the plot,reflects env... "Carbon neutrality movies"are movies that focus on carbon neutrality as the object of expression and dissemination.Using carbon neutrality as an element,it influences the development of the plot,reflects environmental changes,and focuses on climate change caused by carbon emissions.At the same time,it focuses on offsetting carbon emissions through carbon neutrality behavior,showcasing the impact of carbon neutrality.From the perspective of ecological movies,the evolution of carbon neutrality movies at three stages can be explored.The first stage is high-carbon movies that reflect the high conflict between humans and the natural environment.The second stage is low-carbon movies,reflecting humanity's pursuit of a harmonious coexistence between humans and nature,thus adopting green and low-carbon behaviors.The third stage is carbon neutrality movies,which awaken or guide the public to pay attention to carbon emissions,promote low-carbon living,guide life practice in a carbon neutrality way,and create a better life.There are three characteristics of"carbon neutrality movies",including scientific reflection on global warming,advocating energy conservation and emission reduction in daily life,and promoting clean energy in policies. 展开更多
关键词 Carbon neutrality MOVIE evolution characteristic
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Personalized treatment of perihilar cholangiocarcinoma based on tumor genetic and molecular characteristics
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作者 He-Nan Tang Ming-Wei Wang +1 位作者 Xue-Song Liu Yan Jiao 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第9期2769-2773,共5页
This editorial discusses the article written by Tchilikidi et al that was published in the latest edition of the World Journal of Gastrointestinal Surgery.Genetic and molecular profiling of perihilar cholangiocarcinom... This editorial discusses the article written by Tchilikidi et al that was published in the latest edition of the World Journal of Gastrointestinal Surgery.Genetic and molecular profiling of perihilar cholangiocarcinoma(pCCA)has identified a number of key abnormalities that drive tumor growth and spread,including pyruvate kinase M2,proline rich 11,and transcription factor 7,etc.pCCA has specific genetic and molecular features that can be used to develop personalized treatment plans.Personalized treatment approaches offer new opportunities for effectively targeting the underlying drivers of tumor growth and progression.The findings based on tumor genetic and molecular characteristics highlight the importance of developing personalized treatment strategies. 展开更多
关键词 Perihilar cholangiocarcinoma molecular characteristics Tumor genetic PERSONALIZED TREATMENT
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Failure characteristics and the damage evolution of a composite bearing structure in pillar-side cemented paste backfilling 被引量:2
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作者 Boqiang Cui Guorui Feng +6 位作者 Jinwen Bai Gaili Xue Kai Wang Xudong Shi Shanyong Wang Zehua Wang Jun Guo 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2023年第8期1524-1537,共14页
A backfilling body-coal pillar-backfilling body(BPB)structure formed by pillar-side cemented paste backfilling can bear overburden stress and ensure safe mining.However,the failure response of BPB composite samples mu... A backfilling body-coal pillar-backfilling body(BPB)structure formed by pillar-side cemented paste backfilling can bear overburden stress and ensure safe mining.However,the failure response of BPB composite samples must be investigated.This paper examines the deformation characteristics and damage evolution of six types of BPB composite samples using a digital speckle correlation method under uniaxial compression conditions.A new damage evolution equation was established on the basis of the input strain energy and dissipated strain energy at the peak stress.The prevention and control mechanisms of the backfilling body on the coal pillar instability were discussed.The results show that the deformation localization and macroscopic cracks of the BPB composite samples first appeared at the coal-backfilling interface,and then expanded to the backfilling elements,ultimately appearing in the coal elements.The elastic strain energy in the BPB composite samples reached a maximum at the peak stress,whereas the dissipated energy continued to accumulate and increase.The damage evolution curve and equation agree well with the test results,providing further understanding of instability prevention and the control mechanisms of the BPB composite samples.The restraining effect on the coal pillar was gradually reduced with decreasing backfilling body element's volume ratio,and the BPB composite structure became more vulnerable to failure.This research is expected to guide the design,stability monitoring,instability prevention,and control of BPB structures in pillar-side cemented paste backfilling mining. 展开更多
关键词 backfilling body-coal pillar-backfilling body composite structure digital speckle correlation method uniaxial compression deformation characteristics damage evolution
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Tectonic evolution and accumulation characteristics of Carboniferous shale gas in Yadu-Ziyun-Luodian aulacogen, Guizhou Province, South China 被引量:2
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作者 Kun Yuan Wen-hui Huang +5 位作者 Ting Wang Shi-zhen Li Xiang-can Sun Xin-xin Fang Jun-ping Xiao Jun Guo 《China Geology》 CAS CSCD 2023年第4期646-659,共14页
The Yadu-Ziyun-Luodian aulacogen(YZLA) developed into being NW-trending in the Late Paleozoic,and was considered as an important passive continental margin aulacogen in Guizhou Province, South China. This tectonic zon... The Yadu-Ziyun-Luodian aulacogen(YZLA) developed into being NW-trending in the Late Paleozoic,and was considered as an important passive continental margin aulacogen in Guizhou Province, South China. This tectonic zone is considered a large intracontinental thrust-slip tectonic unit, which has undergone a long period of development. It was ultimately determined in the Yanshanian, where the typical Upper Paleozoic marine shales were deposited. In 2021, Well QSD-1 was deployed in the Liupanshui area at the northwest margin of the aulacogen, and obtained a daily shale gas flow of 11011 m3in the Carboniferous Dawuba Formation. It thus achieved a breakthrough in the invesgation of shale gas in the Lower Carboniferous in South China, revealing relatively good gas-bearing properties and broad exploration prospects of the aulacogen. Being different from the Lower Paleozoic strata in the Sichuan Basin and the Yichang area of the Middle Yangtze, the development of the Carboniferous Dawuba Formation in the aulacogen exhibits the following characteristics:(1) The Lower Carboniferous shale is thick and widely distributed, with interbedded shale and marlstone of virous thickness;(2) The total organic carbon(TOC) content of the shale in the Dawuba Formation ranges from 1% to 5%, with an average of 2%, and the thermal maturity of organic matter(Ro) varies from 1% to 4%, with an average of2.5%, indicating good hydrocarbon generation capacity;(3) The main shale in the aulacogen was formed during the fault subsidence stage from the Middle Devonian to the Early Permian. Although the strong compression and deformation during the late Indosinian-Himalayan played a certain role in destroying the formed shale gas reservoirs, comparative analysis suggests that the area covered by the current Triassic strata has a low degree of destruction. It therefore provides good conditions for shale gas preservation,which can be regarded as a favorable area for the next exploration. 展开更多
关键词 Shale gas AULACOGEN CARBONIFEROUS Shale and marlstone Organic carbon Organic matter Hydrocarbon generation capacity Tectonic evolution Accumulation characteristics
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Micro-macro evolution of mechanical behaviors of thermally damaged rock:A state-of-the-art review 被引量:1
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作者 Yunmin Wang Jun Peng +2 位作者 Linfei Wang Chuanhua Xu Bibo Dai 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第7期2833-2853,共21页
The influence of thermal damage on macroscopic and microscopic characteristics of different rocks has received much attention in the field of rock engineering.When the rocks are subjected to thermal treatment,the chan... The influence of thermal damage on macroscopic and microscopic characteristics of different rocks has received much attention in the field of rock engineering.When the rocks are subjected to thermal treatment,the change of macroscopic characteristics and evolution of micro-structure would be induced,ultimately resulting in different degrees of thermal damage in rocks.To better understand the thermal damage mechanism of different rocks and its effect on the rock performance,this study reviews a large number of test results of rock specimens experiencing heating and cooling treatment in the laboratory.Firstly,the variations of macroscopic behaviors,including physical parameters,mechanical parameters,thermal conductivity and permeability,are examined.The variations of mechanical parameters with thermal treatment variables(i.e.temperature or the number of thermal cycles)are divided into four types.Secondly,several measuring methods for microstructure,such as polarizing microscopy,fluorescent method,scanning electron microscopy(SEM),X-ray computerized tomography(CT),acoustic emission(AE)and ultrasonic technique,are introduced.Furthermore,the effect of thermal damage on the mechanical parameters of rocks in response to different thermal treatments,involving temperature magnitude,cooling method and thermal cycle,are discussed.Finally,the limitations and prospects for the research of rock thermal damage are proposed. 展开更多
关键词 Thermal damage Macroscopic characteristics Microstructure evolution Temperature magnitude Cooling method Thermal cycle
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Research on the Evolution of Media Practices of Leading Cadres’ New Media and the Communication Characteristics
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作者 Hebin Zhang Muhammad Zaffwan Idris 《Social Networking》 2023年第3期57-66,共13页
Purpose/Significance: This study aims to define the concept of leading cadres new media usage in China specifically, and to describe and analyze the forms and typical cases and characteristics of leading cadres new me... Purpose/Significance: This study aims to define the concept of leading cadres new media usage in China specifically, and to describe and analyze the forms and typical cases and characteristics of leading cadres new media in different stages. Method/Process: Based on the themes and contents of a few media accounts of leading cadres at different ranks, we summarizes leading cadres new media usage in a systematic perspective and content analysize method. Result/Conclusion: The study defines the concept of Leading Cadres’ new media from the perspective of media practice, determine its position as a media communication platform in the government affairs new media communication system and its relationship with the other elements, and then systematically review the evolution of its media practice from personal website, blog, microblog and microblog to WeChat, live show. On this basis, with the Harold Lasswell communication model, this article summarizes leading cadres new media communication characteristics from five aspects: content producer, content, channel, audience, and effect. Future studies may also enhance the empirical research from the effect perspective. 展开更多
关键词 Leading Cadres’ New Media evolution of Media Practices Communication characteristics
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Molecular Biomarker Characteristics of the Linxi Formation Source Rocks in the Middle-Western Region of Inner Mongolia:New evidence for late-stage tectonic evolution of the Paleo-Asian Ocean 被引量:1
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作者 ZHANG Yongsheng PENG Yuan +3 位作者 SHI Lizhi XING Enyuan GUI Baolin LI Kai 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2017年第2期745-746,共2页
Objective Two important geological issues have long been controversial in the Xing-Meng area of North China. The first concerns the final closure of Paleo-Asian Ocean in Xing-Meng area, and the other concerns the fol... Objective Two important geological issues have long been controversial in the Xing-Meng area of North China. The first concerns the final closure of Paleo-Asian Ocean in Xing-Meng area, and the other concerns the folding and lifting of the Xing-Meng Trough. The focus of thses issues is the Late Permian sedimentary environment, which is generally considered to be either an exclusively continental environment or from the closed inland sea environment in the Early to Middle stage to continental lacustrine environment in the late stage. In recent years, we have successively discovered abundant typical marine fossils (e.g., bryozoans and calcareous algae) in the Upper Permian thick limestone layer from Linxi County and Ar Horqin Banner in eastern region of Inner Mongolia and Jiutain County in Jilin Province. These significant findings have attracted the attention from fellow academics. 展开更多
关键词 In As molecular Biomarker characteristics of the Linxi Formation Source Rocks in the Middle-Western Region of Inner Mongolia:New evidence for late-stage tectonic evolution of the Paleo-Asian Ocean
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Experimental investigation of spectral evolution in flash radiation by hypervelocity impact on aluminum plates
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作者 Xing Chen Yonggang Lu +1 位作者 Zhiwen Li Zhonghua Cui 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第7期96-110,共15页
In this study,a series of hypervelocity impact tests were carried out based on a two-stage light gas gun,and the sequence spectrum and radiation evolution data of the impact products under different impact conditions ... In this study,a series of hypervelocity impact tests were carried out based on a two-stage light gas gun,and the sequence spectrum and radiation evolution data of the impact products under different impact conditions were obtained.The diameter of the projectile is 3-5 mm,the impact velocity is 3.13-6.58 km/s,and the chamber pressure is 0.56-990 Pa.The spectrum of ejected debris cloud in the 250-310 nm band were obtained using a transient spectral measurement system and a multi-channel radiometer measurement system.The test results reveal that the flash radiation intensity increases as a power function with the kinetic energy of the impact.Furthermore,the peak value of the line spectrum decreases as the chamber vacuum degree increases,while the radiation width gradually expands.The line spectrum in the spectral characterization curve corresponds to the ejected debris clouds splitting phase,which does not produce significant line spectrum during material fragmentation and is dominated by the continuum spectrum produced by blackbody radiation.There will appear one or three characteristic peaks in the flash radiation time curve,the first and second peaks correspond to the penetration phase and the third peak corresponds to the expansion phase of the ejected debris clouds on the time scale,the first and second peaks are more sensitive to the chamber vacuum degree,and when the pressure is higher than 99 Pa,the first and second characteristic peaks will disappear.The radiant heat attenuation of the flash under different impact conditions is significantly different,the attenuation exponent has a power function relationship with the impact velocity and the chamber vacuum degree,while the attenuation exponent has a linear relationship with the diameter of the projectile,the specific expression of the attenuation exponent is obtained by fitting.The findings from this research can serve as a valuable reference for remote diagnostic technologies based on flash radiation characteristics. 展开更多
关键词 Hypervelocity impact Flash radiation evolution Spectral characteristics Damage evaluation
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Superposition of dual electric fields in covalent organic frameworks for efficient photocatalytic hydrogen evolution
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作者 Chao Li Shuo Wang +8 位作者 Yuan Liub Xihe Huang Yan Zhuang Shuhong Wu Ying Wang Na Wen Kaifeng Wu Zhengxin Ding Jinlin Long 《Chinese Journal of Catalysis》 SCIE CAS CSCD 2024年第8期164-175,共12页
Covalent organic frameworks(COFs)are promising materials for converting solar energy into green hydrogen.However,limited charge separation and transport in COFs impede their application in the photocatalytic hydrogen ... Covalent organic frameworks(COFs)are promising materials for converting solar energy into green hydrogen.However,limited charge separation and transport in COFs impede their application in the photocatalytic hydrogen evolution reaction(HER).In this study,the intrinsically tunable internal bond electric field(IBEF)at the imine bonds of COFs was manipulated to cooperate with the internal molecular electric field(IMEF)induced by the donor-acceptor(D-A)structure for an efficient HER.The aligned orientation of IBEF and IMEF resulted in a remarkable H_(2) evolution rate of 57.3 mmol·g^(-1)·h^(-1)on TNCA,which was approximately 520 times higher than that of TCNA(0.11 mmol·g^(-1)·h^(-1))with the opposing electric field orientation.The superposition of the dual electric fields enables the IBEF to function as an accelerating field for electron transfer,kinetically facilitat-ing the migration of photogenerated electrons from D to A.Furthermore,theoretical calculations indicate that the inhomogeneous charge distribution at the C and N atoms in TNCA not only pro-vides a strong driving force for carrier transfer but also effectively hinders the return of free elec-trons to the valence band,improving the utilization of photoelectrons.This strategy of fabricating dual electric fields in COFs offers a novel approach to designing photocatalysts for clean energy synthesis. 展开更多
关键词 Covalent organic framework Internal molecular electric field Internal bond electric field PHOTOCATALYSIS Hydrogen evolution
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Unstable evolution of railway slope under the rainfall-vibration joint action
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作者 DONG Haoyu WANG Jiading +2 位作者 ZHANG Dengfei LI Lin XU Yuanjun 《Journal of Mountain Science》 SCIE CSCD 2024年第4期1419-1431,共13页
Understanding the unstable evolution of railway slopes is the premise for preventing slope failure and ensuring the safe operation of trains.However,as two major factors affecting the stability of railway slopes,few s... Understanding the unstable evolution of railway slopes is the premise for preventing slope failure and ensuring the safe operation of trains.However,as two major factors affecting the stability of railway slopes,few scholars have explored the unstable evolution of railway slopes under the joint action of rainfall-vibration.Based on the model test of sandy soil slope,the unstable evolution process of slope under locomotive vibration,rainfall,and rainfall-vibration joint action conditions was simulated in this paper.By comparing and analyzing the variation trends of soil pressure and water content of slope under these conditions,the change laws of pore pressure under the influence of vibration and rainfall were explored.The main control factors affecting the stability of slope structure under the joint action conditions were further defined.Combined with the slope failure phenomena under these three conditions,the causes of slope instability resulting from each leading factor were clarified.Finally,according to the above conclusions,the unstable evolution of the slope under the rainfall-vibration joint action was determined.The test results show that the unstable evolution process of sandy soil slope,under the rainfall-vibration joint action,can be divided into:rainfall erosion cracking,vibration promotion penetrating,and slope instability sliding three stages.In the process of slope unstable evolution,rainfall and vibration play the roles of inducing and promoting slide respectively.In addition,the deep cracks,which are the premise for the formation of the sliding surface,and the violent irregular fluctuation of soil pressure,which reflects the near penetration of the sliding surface,constitute the instability characteristics of the railway slope together.This paper reveals the unstable evolution of sandy soil slopes under the joint action of rainfall-vibration,hoping to provide the theoretical basis for the early warning and prevention technology of railway slopes. 展开更多
关键词 Rainfall vibration joint action Small scale model tests Unstable evolution process Unstable characteristics Inducing sliding and promoting sliding
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Molecular Composition and Evolution of the Chalcone Synthase (CHS) Gene Family in Five Species of Camellia (Theaceae) 被引量:5
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作者 杨俊波 田欣 +1 位作者 李德铢 郭振华 《Acta Botanica Sinica》 CSCD 2003年第6期659-666,共8页
The molecular composition and evolution of the chalcone synthase (CHS) gene family from five species in Camellia (Theaceae) are explored in this study. Sixteen CHS exon 2 from four Camellia species were amplified from... The molecular composition and evolution of the chalcone synthase (CHS) gene family from five species in Camellia (Theaceae) are explored in this study. Sixteen CHS exon 2 from four Camellia species were amplified from total DNA by PCR method. Three sequences of the fifth species in Camellia and two sequences of Glycine max as the designated outgroups were obtained from GenBank. Our results indicated that CHS gene family in Camellia was differentiated to three subfamilies (A, B, C) during the evolutionary history with six groups (A1, A2, A3, BI, B2, C). Among them, only group A2 was possessed by all five species in this study. However, the other five groups were detected only in some species of the plants studied. All members of CHS gene family in this study had high sequence similarity, more than 90% among the members in the same subfamily and more than 78% among different subfamilies at nucleotide level., According to the estimated components of amino acids, the function of CHS genes in Camellia had been diverged. The nucleotide substitutions of the different groups were not identical. Based on phylogenetic analyse inferred from sequences of CHS genes and their deduced amino acid sequences, we concluded that the CHS genes with new function in this genus were evolved either by mutations on several important sites or by accumulation of the mutations after the gene duplication. A further analysis showed that the diversification of CHS genes in Camellia still occurred recently, and the evolutionary models were different to some extant among different species. So we assumed that the different evolutionary models resulted from the impacts of variable environmental elements after the events of speciation. 展开更多
关键词 CAMELLIA chalcone synthase (CHS) gene family molecular evolution
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Influence of rapid solidification on Sn-8Zn-3Bi alloy characteristics and microstructural evolution of solder/Cu joints during elevated temperature aging 被引量:2
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作者 赵国际 文光华 盛光敏 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2017年第1期234-240,共7页
The effects of rapid solidification on the microstructure and melting behavior of the Sn-8Zn-3Bi alloy were studied. The evolution of the microstructuraI characteristics of the solder/Cu joint after an isothermal agin... The effects of rapid solidification on the microstructure and melting behavior of the Sn-8Zn-3Bi alloy were studied. The evolution of the microstructuraI characteristics of the solder/Cu joint after an isothermal aging at 150 ℃ was also analyzed to evaluate the interconnect reliability. Results showed that the Bi in Sn-8Zn-3Bi solder alloy completely dissolved in the Sn matrix with a dendritic structure after rapid solidification. Compared with as-solidified Sn-8Zn-3Bi solder alloy, the melting temperature of the rapid solidified alloy rose to close to that of the Sn-Zn eutectic alloy due to the extreme dissolution of Bi in Sn matrix. Meanwhile, the adverse effect on melting behavior due to Bi addition was decreased significantly. The interfacial intermetallic compound (IMC) layer of the solder/Cu joint was more compact and uniform. Rapid solidification process obviously depressed the formation and growth of the interfacial IMC during the high-temperature aging and improved the high-temperature stability of the Sn-8Zn-3Bi solder/Cu joint. 展开更多
关键词 rapid solidification Sn-8Zn-3Bi solder melting characteristic AGING microstructural evolution
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Molecular evolution of hepatitis A virus in a human diploid cell line 被引量:3
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作者 Cai-Hua Tang Jiang-Sen Mao +2 位作者 Shao-Ai Chai Yong Chen Fang-Cheng Zhuang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第34期4630-4635,共6页
AIM: To investigate the hoLspoLs, direction, and the time course of evolution of hepatitis A virus in the process of consecutive cell culture passage in human KMB17 diploid cells.METHODS: Wild type hepatitis A virus... AIM: To investigate the hoLspoLs, direction, and the time course of evolution of hepatitis A virus in the process of consecutive cell culture passage in human KMB17 diploid cells.METHODS: Wild type hepatitis A virus H2w was serially propagated in KMB17 cells until passage 30, and the fulllength genomes of H2w and its six chosen progenies were determined by directly sequencing RT-PCR products amplified from viral genomic RNA. Alignment comparison of sequences from H2w with its six progenies and phylogenetic analysis of the whole VP1 region from H2w, progenies of H2w, and other cell culture adapted hepatitis A virus were then carried out to obtain data on the molecular evolution of hepatitis A virus in the process of consecutive passage in KMB17 cells.RESULTS: Most of the mutations occurred by passage 5 and several hotspots related to adaptation of the virus during cell growth were observed. After that stage, few additional mutations occurred through the remaining duration of passage in KMB17 cells except for mutation in the virulence determinants, which occurred in the vicinity of passage 15. The phylogenetic analysis of the whole VP1 region suggested that the progenies of H2w evolved closely to other cell culture adapted hepatitis A virus, i.e. MBB, L-A-l, other than its progenitor H2w.CONCLUSION: Hepatitis A virus served as a useful model for studying molecular evolution of viruses in a given environment. The information obtained in this study may provide assistance in cultivating the next generation of a seed virus for live hepatitis A vaccine production. 展开更多
关键词 molecular evolution Virus evolution Phylogenyanalysis Virulence gene Hepat’s A virus
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Sequence and Molecular Evolution Analysis of Ubiquitin Proteins Encoded by Baculoviruses
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作者 郭忠建 朱颖敏 陈克平 《Agricultural Science & Technology》 CAS 2010年第9期53-57,共5页
[Objective] The aim of this study was to analyze the sequence characteristics and molecular evolution of ubiquitins encoded by baculoviruses.[Methods]Clustal W software was used for multiple sequence alignment analysi... [Objective] The aim of this study was to analyze the sequence characteristics and molecular evolution of ubiquitins encoded by baculoviruses.[Methods]Clustal W software was used for multiple sequence alignment analysis,and neighbor-joining method(NJ)and maximum parsimony method(MP)were used for the construction of phylogenetic tree.[Results]The baculoviral ubiquitins showed 73%-86% sequence identity to eukaryotic ubiquitin.Two heterogeneous regions of baculoviral ubiquitins were observed:one was the residues from 15-32,the other was located from residues 53 to 60.The else parts were conserved,where many functional amino acids were also observed.Phylogenetic analysis indicated that baculoviral ubiquitins could be divided into three sub-families,including sub-family GV,sub-family I and sub-family II.The molecular evolution of baculoviral ubiquitins might be under negative selection to maintain their functional and structural stability.[Conclusion]The analysis had provided reference for the researches on functional characterization of baculoviral ubiquitins. 展开更多
关键词 BACULOVIRUS UBIQUITIN SEQUENCE molecular evolution
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The Temporal and Spatial Evolution Characteristics Analysis of Jiangsu Tourism Economy
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作者 张学文 丁正山 《Journal of Landscape Research》 2010年第2期82-86,共5页
[Objective] The paper aimed to reflect the spatial pattern and temporal and spatial evolution characteristics, the differences between inter-regional tourism economy was measured from the quality aspect, which provide... [Objective] The paper aimed to reflect the spatial pattern and temporal and spatial evolution characteristics, the differences between inter-regional tourism economy was measured from the quality aspect, which provided a reference for the local governments in the future tourism development. [Method] Using the location entropy methods, three time periods side of tourism-related date of 2000, 2005, 2007 were selected, from the angle of the spatial pattern and the evolution of the differences within the different scales, the spatial and temporal evolution characteristics of the economic development level of Jiangsu were analysed. [Result] The results showed that from the aspect of spatial evolution pattern, as time goes on, the economic development of Jiangsu tourism has experienced morphological evolution of concentration- dispersion decrease-stability; when it comes to the development of the tourism economy, in recent years, the overall gap between the tourism economy in Jiangsu did not widen, the gap mainly led by the region one after another. According to their volatility, it will be divided into four categories: A Stable type (Wuxi, Xuzhou, Lianyungang and Taizhou), B Increasing type (Huai’an), C Fluctuations type (Nanjing, Changzhou, Suzhou and Yangzhou) and D Depression type (Nantong, Yancheng, Zhenjiang and Suqian). [Conclusion] Location entropy was quoted into tourism economic analysis, the method was simple and easy to understand, the result was accurate and convincing, which provided a reference for travel economic development and investment decision-making of Jiangsu. 展开更多
关键词 Location entropy Tourist economy Temporal and Spatial evolution characteristics Jiangsu
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Deformation and failure characteristics and fracture evolution of cryptocrystalline basalt 被引量:5
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作者 Zhenjiang Liu Chuanqing Zhang +3 位作者 Chunsheng Zhang Yang Gao Hui Zhou Zhaorong Chang 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2019年第5期990-1003,共14页
Cryptocrystalline basalt is one of the two major types of rocks exposed in the super large-scale underground powerhouse in Baihetan hydropower station in China.The rock of this type shows various sitespecific mechanic... Cryptocrystalline basalt is one of the two major types of rocks exposed in the super large-scale underground powerhouse in Baihetan hydropower station in China.The rock of this type shows various sitespecific mechanical responses(e.g.fragmentation,fracturing,and relaxation)during excavation.Using conventional triaxial testing facility MTS 815.03,we obtained the stressestrain curves,macroscopic failure characteristics,and strength characteristics of cryptocrystalline basalt.On this basis,evolution of crack initiation and propagation was explored using the finite-discrete element method(FDEM)to understand the failure mechanism of cryptocrystalline basalt.The test results showed that:(1)under different confining stresses,almost all the pre-peak stressestrain curves of cryptocrystalline basalt were linear and the post-peak stresses decreased rapidly;(2)the cryptocrystalline basalt showed a failure mode in a form of fragmentation under low and medium confining stresses while fragmentation-shear coupling failure dominated at high confining stresses;and(3)the initial strength ratio(sci/sf,where sci and sf are the crack initiation strength and peak strength,respectively)ranged from 0.45 to 0.55 and the damage strength ratio(scd/sf,where scd is the crack damage strength)exceeded 0.9.The stressestrain curve characteristics and failure modes of cryptocrystalline basalt could be reflected numerically.For this,FDEM simulation was employed to reveal the characteristics of cryptocrystalline basalt,including high scd/sf values and rapid failure after scd,with respect to the microscopic characteristics of mineral structures.The results showed that the fragmentation characteristics of cryptocrystalline basalt were closely related to the development of tensile cracks in rock samples prior to failure.Moreover,the decrease in degree of fragmentation with increasing confining stress was also correlated with the dominant effect of confining stress on the tensile cracks. 展开更多
关键词 Baihetan HYDROPOWER STATION CRYPTOCRYSTALLINE BASALT Deformation and failure characteristicS characteristic strengths CRACK propagation evolution
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