We used a proteomic approach to identify IbpA in Cronobacter sakazokii （C. sakazaki）, which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat sh...We used a proteomic approach to identify IbpA in Cronobacter sakazokii （C. sakazaki）, which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat shock. C sakazakii CMCC 45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 AibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazakii CMCC 45402 AibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E. coil O157：H7 strain CMCC 44828. This study suggests that the absence of a single ibpA gene has no obvious effect on the phenotype or heat resistance of the strain C. sakazakii CMCC 45402.展开更多
Particle induced X- ray emission (PIXE) and Rutherford backscattering spectrometry (RBS) have been used extensively for analytical purpose because of their quantitative accuracy, reliability, simplicity and capability...Particle induced X- ray emission (PIXE) and Rutherford backscattering spectrometry (RBS) have been used extensively for analytical purpose because of their quantitative accuracy, reliability, simplicity and capability of non- destructive and multielement analysis. When these techniques are combined with a scanning microbeam system and a data acquisition system, three dimensional distribution of elemental composition can be displayed. Samples analyzed so far at Fudan University include a microelectronic circuit and some biological and archaeological samples. The PIXE and RBS spectra and the secondary electron images have been measured.展开更多
The selection of optimal intensity measures(IMs)has been recommended for generating the seismic demand models with different probabilities by researchers since the seismic IMs are closely associated with earthquake ri...The selection of optimal intensity measures(IMs)has been recommended for generating the seismic demand models with different probabilities by researchers since the seismic IMs are closely associated with earthquake risks and structural safety.However,the seismic design code(mainly for aboveground structures)and dynamic analysis of underground structures conventionally employ the peak ground acceleration(PGA)as an optimal IM.In this paper,the research is to identify the optimal scalar and vector IMs in the fragility investigation of deep-buried hydraulic arched tunnels using the finite element method.A refinement process was performed to determine the optimal scalar IMs by comprehensively comparing their correlation,efficiency,practicality,proficiency,and sufficiency among the examined IMs.Furtherly,the optimum vector IMs were also developed,followed by the three different scalar IMs.Eventually,the dif-ferences between the fragility curves of the tunnel produced using the optimal scalar and vector IM were compared.The generated vector fragility surface can be used to estimate the seismic fragility of identical hydraulic tunnels in an approximative manner.展开更多
During the annual sessions of the National People’s Congress (NPC) and Chinese People’s Political Consultative Conference (CPPCC) this year, NPC deputies and CPPCC members shared their viewpoints on some hot topics ...During the annual sessions of the National People’s Congress (NPC) and Chinese People’s Political Consultative Conference (CPPCC) this year, NPC deputies and CPPCC members shared their viewpoints on some hot topics related to society and people’s livelihoods. Excerpts follow:展开更多
基金funded by National Science and Technology Major Project of the Ministry of Science and Technology of China(2013ZX09304101)
文摘We used a proteomic approach to identify IbpA in Cronobacter sakazokii （C. sakazaki）, which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat shock. C sakazakii CMCC 45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 AibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazakii CMCC 45402 AibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E. coil O157：H7 strain CMCC 44828. This study suggests that the absence of a single ibpA gene has no obvious effect on the phenotype or heat resistance of the strain C. sakazakii CMCC 45402.
文摘Particle induced X- ray emission (PIXE) and Rutherford backscattering spectrometry (RBS) have been used extensively for analytical purpose because of their quantitative accuracy, reliability, simplicity and capability of non- destructive and multielement analysis. When these techniques are combined with a scanning microbeam system and a data acquisition system, three dimensional distribution of elemental composition can be displayed. Samples analyzed so far at Fudan University include a microelectronic circuit and some biological and archaeological samples. The PIXE and RBS spectra and the secondary electron images have been measured.
基金support from the National Natural Science Foundation of China(Grant No.52209169).
文摘The selection of optimal intensity measures(IMs)has been recommended for generating the seismic demand models with different probabilities by researchers since the seismic IMs are closely associated with earthquake risks and structural safety.However,the seismic design code(mainly for aboveground structures)and dynamic analysis of underground structures conventionally employ the peak ground acceleration(PGA)as an optimal IM.In this paper,the research is to identify the optimal scalar and vector IMs in the fragility investigation of deep-buried hydraulic arched tunnels using the finite element method.A refinement process was performed to determine the optimal scalar IMs by comprehensively comparing their correlation,efficiency,practicality,proficiency,and sufficiency among the examined IMs.Furtherly,the optimum vector IMs were also developed,followed by the three different scalar IMs.Eventually,the dif-ferences between the fragility curves of the tunnel produced using the optimal scalar and vector IM were compared.The generated vector fragility surface can be used to estimate the seismic fragility of identical hydraulic tunnels in an approximative manner.
文摘During the annual sessions of the National People’s Congress (NPC) and Chinese People’s Political Consultative Conference (CPPCC) this year, NPC deputies and CPPCC members shared their viewpoints on some hot topics related to society and people’s livelihoods. Excerpts follow:
