MECHANISM OF CHANGE IN THE CATION INDUCED EXCITA TION ENERGY DISTRIBUTION BETWEEN PSⅡ AND PSⅠ IN THE CHLOROPLAST MEMBRANE FROM ZOSTERA MARINA *

The interrelations between thylakoid polypeptide components and Mg 2+ induced Chl a fluorescence and thylakoid surface charge changes were investigated in Zostera marina chloroplasts treated with Ca 2+ and trypsin. It was observed that:1. The increase of Mg 2+ induced PSⅡ fluorescence intensity was closely related to the decrease of Mg 2+ induced surface charge density of the thylakoid membrane in the normal chloroplast; 2. Removal of the 32~34 kD polypeptides of the thylakoid surface by Ca 2+ extraction of the chloroplast did not affect the Mg 2+ induced phenomena; 3. If the Ca 2+ treated chloroplast was further digested by trypsin to remove the 26 kD polypeptide of the membrane surface, the Mg 2+ induced phenomena disappeared completely. These results clearly indicated that the 26 kD polypeptide of thylakoid surface is the specific acting site of the cation that induced these two correlated phenomena in the chloroplast from Zostera marina. The mechanism on the regulating effect of the cation on excitation energy distribution between PSⅡ and PSⅠ was discussed.

Effects of High Light Stress on Chlorophyll-protein Complexes of Two Subspecies of Rice

Influence of high light stress on the photosynthesis of flag leaves of indica subspecies (cv. “Shanyou 63', sensitive to photoinhibition) and japonica subspecies (cv. “Wuyujing', resistant to photoinhibition) of rice ( Oryza sativa L.) was comparatively investigated. In both cultivars of rice, the excitation energy distribution between two photosystems was altered and the excitation energy transfer from light harvesting chlorophyll protein complexes to PSⅡ was inhibited by high light stress. These decreases were more pronounced in indica rice cultivar as compared to japonica one. The analysis of mild SDS_PAGE showed that in indica rice, high light stress almost disaggregated the trimer of light harvesting chlorophyll protein complexes of PSⅡ (LHC Ⅱ 1). The stress reduced the contents of internal antennae chlorophyll protein complexes of PSⅡ (CPa), light harvesting chlorophyll protein of PSⅠ (CPⅠa) and Chl a protein complex of PSⅠ reaction center (CPⅠ) as well as dimer of LHCⅡ (LHCⅡ 2) in indica rice. In japonica subspecies, however, high light stress depressed the contents of LHCⅡ 1, CPa and CPⅠa, but slightly impacted on CPⅠ content. Moreover, the increase in the contents of monomer of LHCⅡ by high light stress was found in both subspecies. In consistent with above results, analysis of polypeptide indicated that the amounts of 27 kD and 25 kD polypeptide of LHCⅡ in particular, as well as that of 21 kD polypeptide of CPⅠa were reduced by high light stress in both subspecies. It was found that, comparing with japonica rice, the stress pronouncedly diminished 43 kD and 47 kD proteins of CPa and 23 kD extrisic protein in indica rice.

Electron population properties with different energies in a helicon plasma source

The characteristics of electrons play a dominant role in determining the ionization and acceleration processes of plasmas.Compared with electrostatic diagnostics,the optical method is independent of the radio frequency(RF)noise,magnetic field,and electric field.In this paper,an optical emission spectroscope was used to determine the plasma emission spectra,electron excitation energy population distributions(EEEPDs),growth rates of low-energy and highenergy electrons,and their intensity jumps with input powers.The 56 emission lines with the highest signal-to-noise ratio and their corresponding electron excitation energy were used for the translation of the spectrum into EEEPD.One discrete EEEPD has two clear different regions,namely the low-energy electron excitation region(neutral lines with threshold energy of13–15 eV)and the high-energy electron excitation region(ionic lines with threshold energy?19 e V).The EEEPD variations with different diameters of discharge tubes(20 mm,40 mm,and 60 mm)and different input RF powers(200–1800 W)were investigated.By normalized intensity comparison of the ionic and neutral lines,the growth rate of the ionic population was higher than the neutral one,especially when the tube diameter was less than 40 mm and the input power was higher than 1000 W.Moreover,we found that the intensities of low-energy electrons and high-energy electrons jump at different input powers from inductively coupled(H)mode to helicon(W)mode;therefore,the determination of W mode needs to be carefully considered.