Bone-conditioned medium contributes to initiation and progression of osteogenesis by exhibiting synergistic TGF-β1/BMP-2 activity

Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells. We detected a fast and significant (P <0.001) release of transforming growth factor-β1 (TGF-β1) from autologous bone within 10 min versus a delayed bone morphogenetic protein-2 (BMP-2) release from 40 min onwards. BCMs harvested within short time periods (10, 20, or 40 min), corresponding to the time of a typical surgical procedure, significantly increased the proliferative activity and collagen matrix production of BCM-treated cells. Long-term (1, 3, or 6 days)-extracted BCMs promoted the later stages of osteoblast differentiation and maturation. Short-term-extracted BCMs, in which TGF-β1 but no BMP-2was detected, reduced the expression of the late differentiation marker osteocalcin. However, when both growth factors were present simultaneously in the BCM, no inhibitory effects on osteoblast differentiation were observed, suggesting a synergistic TGF-β1/BMP-2 activity. Consequently, in cells that were co-stimulated with recombinant TGF-β1 and BMP-2, we showed a significant stimulatory and dose-dependent effect of TGF-β1 on BMP-2-induced osteoblast differentiation due to prolonged BMP signaling and reduced expression of the BMP-2 antagonist noggin. Altogether, our data provide new insights into the molecular mechanisms underlying the favorable outcome from GBR procedures using BCM, derived from autologous bone grafts.

Exhibiting Friendship

WANGFUJlNG Street is one of China＇s most renowned shopping streets. The area＇s commercial history can date back many centuries. Today, the street located in the eastern-central side of the city is always choked with happy＇shoppers and tourists from home and abroad. One August morning, people found that the street. congested as usual, had somehow changed - in the middle stood a row of panels which displayed pictures representing another country. ＂The photo exhibition, titled ＇South Africa in Beijing,＇ seeks to showcase the very rich and diverse South African culture and heritage to the broad Chinese public,＂ said Dr. Bheki Langa, South Africa＇s Ambassador to China.

Exhibiting Fine Form

Chinese entrepreneurs can be found scattered across the globe, all with an innate sense of business that has made them a potent force ininternational trade . To illustrate the importance of entrepreneurs, at the 100th Chinese Export Commodities Fair (CECF) held in Guangzhou in October, Premier Wen Jiabao met with Zheng Mingru, Permanent Honorary President of the Wor ld Chinese Entrepr eneurs General Association (WCEGA), and awar ded him a medal for his sterling wor k in connecting Chinese business people wo...

Musical Instrument Exhibition Boosts Integration of Digital Technology and Musical Instrument Industry

On June 27,the 31st China International Exhibition on Music was held at the China International Exhibition Center(Chaoyang Hall).The exhibition area reached 35,000 square meters and attracted more than 40,000 visitors from both home and abroad during the four-day exhibition.As a mega musical exhibition in China,the 31st China International Exhibition on Music integrates musical instrument exhibitions and displays,forums and lectures,instrumental music performances,competitions,communications,music education and so on.

LANTING Chinese Calligraphy Culture Salon Held in Bangkok

China Cultural Center in Bangkok presented the LANTING Chinese Calligraphy Culture Salon in cooperation with academic institutions and universities from late March to the end of April,2024.It organized online and offline activities such as calligraphy classes,Chinese story lectures,pen club salons,"Chinese Culture on Campus"lectures,and online calligraphy exhibitions.Nearly 400 people were directly involved offline and tens of thousands of audiences were reached online.

The Chinese Art Academies and Troupes Facilitate Cultural Exchanges Between China and the World

With the further adjustment of China's entry and exit policy,Chinese art troupes have once again embarked on the journey of overseas performances(exhibitions),infusing a renewed vigor into the cultural exchanges between China and the world.This issue pays special attention to the recent wonderful performances(exhibitions)of Chinese art troupes overseas,and records how they present the charm of the Chinese culture through art—a bridge across national boundaries,and enhance the friendship with people around the world.

The "Garden of Emeralds" Exhibition:Interview with Dr. EloÏse Gaillou,Director and Curator of the Mineralogy Museum of l'école des Mines de Paris(Mines Paris-PSL)

From May 25 to October 7,2024,L’éCOLE China,School of Jewelry Arts(supported by Van Cleef & Arpels) presents the "Garden of Emeralds" exhibition in its Shanghai campus.As part of the "Discover the Gemstones" series,the exhibition gives pride to this green precious mineral and explores its history in many regions of the world,from Asia to Europe including the Middle East.Gathering both raw stones and pieces of jewelry from museum collections and private lenders,it addresses the topic through the three main pillars of L’éCOLE:gemmology,savoir-faire and the history of jewelry.

Splendor in Myriads A Kaleidoscope of Art Treasures from the 16th to the 20th Centuries

Treasures from different regions transcend time and space,shining together in the exhibition hall,immersing the audience in an artistic feast that connects the beauty of Eastern and Western craftsmanship,the charm of history and culture,the spectrum of the evolution of the ages,and the joy of cultural exchanges.

Recent and Forthcoming Events

China Culture Day in Sri Lanka On May 9,the opening ceremony of the"China Culture Day"was held at the Colombo Public Library Hall in Sri Lanka.The"China Culture Day"lasted for two days,including a"Cultural Tourism Photo Exhibition",cultural book donations,Chinese culture classes,calligraphy workshops and so on.

LANTING Chinese Calligraphy Culture Salon Calligraphy Exhibition and Workshop Held in Mexico City

On April 20,the LANTING Chinese Calligraphy Culture Salon Calligraphy Exhibition and Workshop,jointly organized by China Cultural Center in Mexico and the Carranza Museum,was held in Mexico City.The exhibition presents works copied and created by calligraphers such as Fang Fang,Qiu Xiaoqi,Jiang Yan,Shi Yongxin,Wang Hexuan,Dong Jie,Yan Da and Yan Kan,as well as the calligraphy works of Mexican calligraphy enthusiast Miriam and other friends.

RECENT AND FORTHCOMING EVENTS

Asia Special Exhibition on Liangzhu Opens in Singapore On October 19,local time,the Journey Through Civilizations.An Encounter with Liangzhu(2024 World Tours)Singapore Special Exhibition,jointly organized by the Asia Pacific Headquarters of China Central Radio and Television,China Cultural Center in Singapore,the Hangzhou Liangzhu Site Management Committee,and the China Central Radio and Televsion"Yangbo"Digital Culture and Art Museum,opened at China Cultural Center in Singapore.

‘Silk Road Artists’Rendezvous’ Exhibition

The"Silk Road Artists'Rendezvous"exhibition kicked off in Mengqi Town,Yuhang District,Hangzhou,where 152 artworks of 84 artists from 83 countries are on show.As a key sideline event of the Liangzhu Forum,the exhibition is hosted by the Ministry of Culture and Tourism of the People's Republic of China,People's Government of Zhejiang Province,and China International Culture Association(CiCA),and organized by the Bureau of International Exchanges and Cooperation of Ministry of Culture and Tourism of the People's Republic of China,Publicity Department of the CPC Zhejiang Provincial Committee,Zhejiang Provincial Department of Culture and Tourism,Hangzhou Municipal Government,and China Academy of Art(CAA)on December 3,2023 in Hangzhou,Zhejiang Province,China.

A Horticultural Feast in Chengdu

The International Horticultural Exhibition 2024 demonstrates China’s determination to achieve ecological progress.“Behind me,you can see the roof of the main exhibition hall.It is made of one million ginkgo leaves!”Holding a selfie stick,travel vlogger Xiaochuan said while standing at the main exhibition hall of the International Horticultural Exhibition 2024 Chengdu,recording a tour guide for his followers.He was dazzled by the green and low-carbon concepts at the exhibition,which opened on April 26,2024 and will run till October 28.

Experience Zhongshan Where cultural heritage meets the heartbeat of life

As night falls,Sunwen West Road comes alive with the vibrant spectacle of lion dances.Accompanied by rhythmic drum beats,the majestic and colorful lion dancers leap and twirl through the crowd.Spectators gather to watch as the troupe makes its way to Xiangshan Theater,where an eager audience awaits a showcase of traditional performances including the spectacular high-pole lion dance and ceremonial dragon dance.With the newly opened Shenzhen-Zhongshan Link serving as a golden corridor towards prosperity,Zhongshan City in south China’s Guangdong Province seized the opportunity of the first National Day holiday since its opening to curate a series of nearly 200 cultural and tourism events under the theme“Experience the Lifestyle of the Greater Bay Area.”The activities included art performances,intangible cultural heritage shows,exhibitions,and food tastings.

Remembering Soong Ching Ling at the 75th Anniversary of the PRC

An exhibition in Beijing pays tribute to a remarkable woman who was a pillar of China’s revolutionary movement.SOONG Ching Ling,one of the founders of the People’s Republic of China(PRC),passed away in 1981 but her work to support the revolution in China and create a network of loyal friends worldwide for the PRC lives on through the China Soong Ching Ling Foundation(CSCLF).Soong’s life was defined by the struggle for a new China and her prolific writings and speeches have become important documents in Chinese history.

Magazine Review

The Evolutionof Ritesin the East:Ancient Relics of Shandong Reflecting the Brilliant Civilizationof China,In recent years,with the development of China's social economy,people choose more and more modes of leisure during the Chinese New Year holiday.Among them,visiting museums and art galleries has become an important choice for people to pursue spiritual and cultural life and improve their own quality,which has even formed a fashion of"enjoying the happy Chinese New Year at museums and art galleries".During the Chinese New Year,museums and artgalleries across the country launch colorful New Yearexhibitions.

Beauty in the Gathering:Exhibition of Precious Collection from Art Museums Around China

"Beauty in the Gathering:Exhibition of Precious Collection from Art Museums around China"opens at the National Art Museum of China(NAMOC)on February 1 and lasts until April 1.The exhibition provides a glimpse of the great variety of art collected by 15 museums,and distinctive features that vary from region to region,which has witnessed the further prosperity and development of the art museum industry since the new era.The exhibition showcases over 500 pieces of traditional Chinese painting,oil painting,printmaking,watercolor painting,lacquer painting,sculpture,folk art,mixed media art,calligraphy and other collections provided by these museums.

Ink Splendor and Cultural Context Exhibition of Selected Ancient Paintings Collected by Jilin Provincial Museum

Continuing the theme of"ink Splendor and Cultural Context"to celebrate the 6Oth anniversary of the founding of the National Art Museum of China(NAMOC),the Exhibition of Selected Ancient Paintings Collectedby Jilin Provincial Museum was recently unveiled at NAMOC.

Recent and Forthcoming Events

'Nihao!China'VR Beautiful China Tourism Interactive Experience Exhibition Opened in Laos.On June 6,in the multifunction Hall of China Cultural Center in Laos,"Nihao!China"VR Beautiful China Tourism Interactive Experience Exhibition was officially opened in the launch ceremony of exquisite visual effects and full of scientific and technological sense."Nihao!China"VR Beautiful China Tourism Interactive Experience Exhibition is divided into six experience sections.Guests put on VR devices,hold the handle,and travel through the moment to truly feel Chinese culture.