Experimental models of high-risk bowel anastomosis in rats:A systematic review

BACKGROUND Anastomotic leaks remain one of the most dreaded complications in gastrointestinal surgery causing significant morbidity,that negatively affect the patients’quality of life.Experimental studies play an important role in understanding the pathophysiological background of anastomotic healing and there are still many fields that require further investigation.Knowledge drawn from these studies can lead to interventions or techniques that can reduce the risk of anastomotic leak in patients with high-risk features.Despite the advances in experimental protocols and techniques,designing a high-quality study is still challenging for the investigators as there is a plethora of different models used.AIM To review current state of the art for experimental protocols in high-risk anastomosis in rats.METHODS This systematic review was performed according to The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.To identify eligible studies,a comprehensive literature search was performed in the electronic databases PubMed(MEDLINE)and Scopus,covering the period from conception until 18 October 2023.RESULTS From our search strategy 102 studies were included and were categorized based on the mechanism used to create a high-risk anastomosis.Methods of assessing anastomotic healing were extracted and were individually appraised.CONCLUSION Anastomotic healing studies have evolved over the last decades,but the findings are yet to be translated into human studies.There is a need for high-quality,well-designed studies that will help to the better understanding of the pathophysiology of anastomotic healing and the effects of various interventions.

Experimental Study of Confining Pressure Initiated by Tectonic Force

An experimental study of the confining pressure, i.e. additional hydrostatic pressure initiated by the tectonic force is presented. The experimental progress is that the σ 1 is gradually increasing from 0 in a limiting movement ( ε 1=0) in the σ 1 direction and the speed rate of the accelerating load is 0.4 MPa·s -1 in the lateral and level directions. When σ 2= σ 3<200 MPa, Δ σ l is nearly lacking, Δ σ l is increasing at a high speed only when the horizontal force reaches 250-380 MPa, and Δ σ l almost ceases to increase at the level force of 380 MPa. It is calculated that the tectonic force can produce the confining pressure which is gradually increasing with σ 2= σ 3 before it reaches 380 MPa in an experiment. It is supposed that the horizontal force is almost all transformed into the confining pressure with the increase of the creep deformation of rocks.

An Experimental Study on Atmospheric Pressure Glow Discharge in Different Gases

Usually, the electrical breakdown of dielectric barrier discharge (DBD) at atmo-spheric pressure leads to a filamentary non-homogeneous discharge. However, it is also possible to obtain a diffuse DBD in homogeneous form, called atmospheric pressure glow discharge (APGD). We obtained a uniform APGD in helium and in the mixture of argon with alcohol, and studied the electrical characteristics of helium APGD. It is found that the relationship between discharge current and source frequency is different depending on the difference in gas gap when the applied voltage is kept constant. The discharge current shows an increasing trend with the increased frequency when gas gap is 0.8 cm, but the discharge current tends to decrease with the increased frequency when the gas gap increases. The discharge current always increases with the increased applied voltage when the source frequency is kept constant. We also observed a glow-like discharge in nitrogen at atmospheric pressure.

Experimental Study of Pore Water Pressure and Bed Profile Change Under Regular Breaking Waves

There lies a close relationship between the seabed destruction and the distribution of pore water pressure under the action of breaking wave. The experiments were carried out in a wave flume with a 1：30 sloping sandy seabed to study regular breaking wave induced pore water pressure. A wide range of measurements from the regular wave runs were reported, including time series of wave heights, pore pressures. The video records were analysed to measure the time development of the seabed form and the characteristics of the orbital motion of the sand in the wave breaking region. The pore water pressure in the breaker zone showed the time variation depending on the wave phases including wave breaking and bore propagation. The time-averaged pore water pressure was higher near the seabed surface. The peak values of pore water pressure increase significantly at the breaking point. The direction of pore water pressure difference forces in the breaker zone is of fundamental importance for a correct description of the sediment dynamics. The upwards- directed pressure differences may increase sand transport by reducing the effective weight of the sediment, thereby increasing the bed form evolution. The seabed configuration changed greatly at the wave breaking zone and a sand bar was generated remarkably. The amplitude of the pore water pressure changed with the seabed surface. The results are to improve the understanding of sand transport mechanisms and seabed responses due to breaking regular waves over a sloping sandy bed.

An Experimental Study on the Pressure Distribution in Horizontal Gas Wells

Laboratory experiments have been carried out to study the fluid flow in the wellbore of a horizontal gas well during the production process.The related pressure distribution has been determined considering different cases(different inflow media,different perforation opening methods and different liquid holdup).It has been found that the larger the fluid flow rate,the greater the pressure changes in the wellbore under the same hole opening mode.The uniformity of the perforation opening method was also an important factor affecting the magnitude of the wellbore pressure change.The liquid holdup also affected the pressure distribution,especially when the gas volumetric flow rate exceeded 200 m3/h.Comparison of the outcomes of the present experimental study with the predictions of a theoretical model available in the literature has provided a relative error smaller than 20%.

Experimental Verification and Research for the Distortion in the Integrated Frequency Responses of the High-Pressure Sealed Cabin and Magnetic Field Sensor

Although magnetotelluric sounding method applied to the land is advanced, there are many difficulties when it is applied to marine environment, one of which is how to lay magnetic field sensors down to the seafloor to complete measurements. To protect the magnetic field sensors from intense erosion and high pressure, suitable high-pressure sealed cabins must be designed to load them. For the consideration of magnetic measurement and marine operation, the sealed pressure cabin should be nonmagnetic and transportable. Among all optional materials, LC4 super.hard aluminum alloy has the highest performance of price/quality ratio to make the sealed pressure cabin. However, it does not mean that the high-pressure sealed cabin made using LC4 will be perfect in performance. In fact, because of its weak magnetism, the pressure cabin made using LC4 has distorting effect on frequency responses of the magnetic field sensors sealed in it. This distorting effect does not affect the use of the magnetic field sensor, but if we want to eliminate its effect, we should study it by experimental measurements. In our experiment tests, frequency sweep magnetic field as excitation signal was used, and then responses of the magnetic field sensor before and after being loaded into the high-pressure sealed cabin were measured. Finally, normalized abnormal curves for the frequency responses were obtained, through which we could show how the high-pressure sealed cabin produces effects on the responses of the magnetic field sensor. Experimental results suggest that the response distortion induced by the sealed pressure cabin appears on mid- and high-frequency areas. Using experimental results as standardization data, the frequency responses collected from seafloor magnetotelluric measurements can be corrected to restore real information about the seafloor field source.

Experimental Investigation of Unsteady Pressure on an Axial Compressor Rotor Blade Surface

The inherent unsteady pressure fluctuations on the rotating blade suction surface of an axial compressor were experimentally measured by directly mounting five high response miniature pressure transducers into the rotor blade along a streamline at 50% span respectively. The results show that the unsteady pressure fluctuations of rotor blade surface could be measured successfully by this means. The relations about the period, altitude of unsteady pressure with rotating speed, the discipline of pressure fluctuation along the streamwise direction were obtained.

Experimental Observation of Phase Transition in Sb203 under High Pressure

The in situ high-pressure behavior of the semiconductor antimony trioxide （Sb2O3） iS investigated by the Raman spectroscopy techniques and angle-dispersive synchrotron x-ray powder diffractfon in a diamond anvil cell up to 31.5 and 30.7 GPa, respectively. New peaks observed in the external lattice mode range in the Raman spectra at 13.5 GPa suggest that the structural phase transition occurs. The group mode （140 cm^-1） in Sb2O3 exhibits anomalous pressure dependence; that is, the frequency decreases gradually with the increasing pressure. High pressure synchrotron x-ray diffraction measurements at room temperature reveal that the transition from the orthorhombic structure to high-pressure new phase occurs at about 14.2 GPa, corresponding to the softening of the group optic mode （140cm^-1）.

Experimental Analysis on the Variable Polarity Plasma Arc Pressure

Arc pressure is one of the key factors for variable polarity plasma arc（VPPA） and welding pool formation. In this paper, VPPA pressure is measured by pressure transducer and U-tube barometer methods, and advantages and disadvantages of the two methods are compared. The effects of welding parameters, including with straight polarity（SP） current, reverse polarity（RP） current, time ratio of SP to RP, plasma gas flow rate, on VPPA pressure are investigated by using an orthogonal design. The experimental results indicate that the influencing degree of the welding parameters are in the order of plasma gas flow rate, SP current, time ratio of SP to RP, RP current. These results are important to researches of VPPA welding process and its mechanism. The physics behavior of VPPA is taken into account when the above influence mechanisms are analyzed. Firstly, according to the mechanism of the cooling compression to the arc, the compression to VPPA is enhanced with the increase of plasma gas flow, so the VPPA pressure would increase obviously. Secondly, although the temperature of VPPA is as a function of the welding current, the radius of VPPA is also enhanced. So the effects of SP current on VPPA pressure are inferior to the effects of plasma gas flow. Thirdly, VPPA pressure increases as a function of time ratio of SP to RP because the frequency of welding current influences the arc pressure to the some degree; Finally, the RP intervals are farther less than the SP intervals, so the influence to the pressure is minimal.

Experimental Investigation of Pressure Drop Hysteresis in a Cocurrent Gas-Liquid Upflow Packed Bed

Extensive experimental work on hysteresis in a c℃urrent gas-liquid upflow packed bed was carried out with three kinds of packings and the air-water system. However, only when packed with small glass beads ((1.4 mm) was the bed pressure drop hysteresis observed. Two more liquids with different liquid properties were employed to further examine the influence of parameters on pressure drop hysteresis. The similarity of pressure drop hysteresis in packed beds was concluded in combination of experimental evidence reported in literature.

CFD simulation of pressure fluctuation characteristics in the gas-solid fluidized bed:Comparisons with experiments

A simple hydrodynamic model based on two-fluid theory, taking into account the effect of discrete particles on both the gas- and solid-phase momentum equations, was used to numerically investigate the pressure fluctuation characteristics in a gas-solid fluidized bed with the aid of CFX 4.4, a commercial CFD software package, by adding user-defined Fortran subroutines. Numerical simulations together with typical experimental measurements show that pressure fluctuations originate above the distributor when a gas pulse is injected into the fluidized bed. The pressure above the bubble gradually increases due to the presence of a rising bubble. When the bubble passes through the bed surface, the pressure near the bed surface gradually decreases to a lower value. Moreover, the pressure signals in the bubbling fluidized beds show obviously periodic characteristics. The major frequency of pressure fluctuations at the same vertical position is affected slightly by the operating gas velocity, and the amplitude of pressure fluctuations is related to both the operating gas velocity and the vertical height. In this study, the influence of the operating gas velocity on the pressure wave propagation velocity can be ignored, and only two peak frequencies in the power spectrum of the pressure fluctuations are observed which are associated with the bubble formation above the distributor and its eruption at the bed surface.

Atmospheric pressure pulsed modulated arc discharge plasma

Direct-current(DC)arc plasma has great application values in the field of the chemical industry,but it has the problem of low energy efficiency.Facing the requirement for improving the energy efficiency of the arc,this paper proposes a unique method of pulsed modulated arc(PMA).This method uses high-frequency pulses and reduces the arc current to improve the control of electron temperature.The electrical characteristics,optical characteristics and products are tested.The test results show that during the PMA process,all of the experimental results which include voltage,current and light will significantly increase.These results are analyzed from the perspective of functionality,repeatability and energy conversion.The analysis results show that although the PMA method does not show good parameter consistency,it has potential application prospects because it increases the energy conversion rate by 4.5%and 8%from the perspective of light and products,respectively.

Experimental Research on Supercritical Carbon Dioxide Fracturing of Sedimentary Rock:A Critical Review

Supercritical carbon dioxide(ScCO_(2))fracturing has great advantages and prospects in both shale gas exploitation and CO_(2)storage.This paper reviews current laboratory experimental methods and results for sedimentary rocks fractured by ScCO_(2).The breakdown pressure,fracture parameters,mineral composition,bedding plane angle and permeability are discussed.We also compare the differences between sedimentary rock and granite fractured by ScCO_(2),ultimately noting problems and suggesting solutions and strategies for the future.The analysis found that the breakdown pressure of ScCO_(2)was reduced 6.52%–52.31%compared with that of using water.ScCO_(2)tends to produce a complex fracture morphology with significantly higher permeability.When compared with water,the fracture aperture of ScCO_(2)was decreased by 4.10%–72.33%,the tortuosity of ScCO_(2)was increased by 5.41%–70.98%and the fractal dimension of ScCO_(2)was increased by 4.55%–8.41%.The breakdown pressure of sandstone is more sensitive to the nature of the fracturing fluid,but fracture aperture is less sensitive to fracturing fluid than for shale and coal.Compared with granite,the tortuosity of sedimentary rock is more sensitive to the fracturing fluid and the fracture fractal dimension is less sensitive to the fracturing fluid.Existing research shows that ScCO_(2)has the advantages of low breakdown pressure,good fracture creation and environmental protection.It is recommended that research be conducted in terms of sample terms,experimental conditions,effectiveness evaluation and theoretical derivation in order to promote the application of ScCO_(2)reformed reservoirs in the future.

Experiment Research on Fine Regulation of Wheel Cylinder Pressure Using PWM Technology

A test bench of ABS/ASR integrated hydraulic system is developed by using pulse-width modulation （PWM） technology. The effective duty ratio range of ABS outlet valve has been tested in PWM control. With 50 Hz carrier wave frequency, the tests are performed to determine the correspondence between duty ratio and the wheel cylinder pressure variation. The duty ratio range of ABS outlet valve in PWM control is determined and an experimental model of pressure reduction velocity （PRV） of wheel cylinder using PWM control is established. By comparison and test of the experimental model and realization of controlling the duty ratio of ABS outlet valves, the fine regulation of wheel cylinder PRV is realized in the working of ABS/ASR braking regulation, which is important and valuable to the improvement of the ABS/ASR controlling effect.

Influence of specific speed on hydraulic performances and pressure fluctuations in mixed-flow pumps

A series of steady and unsteady numerical calculations of the internal flow in mixed-flow pumps with three different specific speeds were carried out based on the N-S equation coupled with the standard k-εturbulence model under different operating conditions to investigate the relationship between the impeller specific speed and the pump performance as well as pressure pulsations.Meanwhile,the pump performance and pressure pulsations inside the mixed-flow pump with three different specific speeds were also analyzed and compared with the corresponding test data.From the results,the averaged deviations between the predicted and tested head among different impellers are below 5%,and with respect to the equivalent impeller specific speeds of 280 and 260,the values are 4.30%and 3.69%,respectively.For all the impeller schemes,the best efficiency point of the mixed-flow pump is found at the flow rate of 1.2 Q_(d) and the higher head deviation occurs at lower flow rates.Especially,it can be found that the specific speed has a slight effect on the pressure fluctuation in the impellers.Eventually,it is determined that the pump performance curves calculated by numerical simu-lations have good agreement with the relevant experimental results,which verifies that the numerical methods used in the present study are accurate to a certain extent.Furthermore,the results also provide some references to the pressure pulsation analysis and the performance improvement of the mixed-flow pump design.

Protective effects of nerve regeneration factor and brain-derived neurotrophic factor on retinal ganglion cells in a rabbit model of acute hyper-intraocular pressure

BACKGROUND： Previous studies have shown that nerve regeneration factor （NRF） provides neuroprotective effects. However, the neuroprotective effects on retinal ganglion cells in an animal model of glaucoma remain uncertain. OBJECTIVE： To determine the neuroprotective effects of NRF on retinal ganglion cells in a rabbit model of acute hyper-intraocular pressure and to compare the effects on brain-derived neurotrophic factor （BDNF）. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at Jiangsu Provincial Key Laboratory of Neural Regeneration from September 2006 to August 2007. MATERIALS： Sterone, a major component of NRF, was provided by the Key Laboratory of Neural Regeneration, Nantong University in China; BDNF was provided by BioDesign Inc., USA. METHODS： A total of 24 healthy rabbits were randomly assigned to NRF, BDNF, and phosphate buffered saline groups, with 8 rabbits per group. The left eyes were considered normal controls, and acute hyper-intraocular pressure was induced in the right eyes via anterior chamber perfusion. The right camera vitrea bulbi was injected with 4.5 μg NRF, 3.75 μg BDNF, or 5 μL 0.1 mol/L phosphate buffered saline, respectively. MAIN OUTCOME MEASURES： Retinal ganglion cells were reverse-labeled using horseradish peroxidase to quantify cell density at 2, 4, and 6 mm from the optic disc edge. RESULTS： NRF increased the number of surviving retinal ganglion cells at the optic disc edge （P 〈 0.01 or P 〈 0.05）. The density of surviving retinal ganglion cells decreased with increasing distance from the optic disc. The number of retinal ganglion cells in the BDNF group was similar to the NRF group （P 〉 0.05）. At 2, 4, and 6 mm away from the optic disc edge, there was no significant difference in retinal ganglion cell density between NRF and BDNF groups （P〉 0.05）. CONCLUSION： NRF provided protection to retinal ganglion cells in a rabbit model of acute hyper-intraocular pressure, Le., NRF enhanced the survival rate of retinal ganglion cells. The neuroprotective effect was similar to BDNF.

Cold Flow Experiments and Acoustic Investigation on Pressure Oscillation Induced by Flow Instability

Pressure oscillation in solid rocket motor is believed to be the results of the interaction between the flow instability and the acoustics of combustion chamber.Several reasonable and necessary hypothesizes are given to establish an equation to describe this coupling.A cold flow motor called CVS60D(corner vortex shedding 60°)was designed to study the flow-acoustic coupling based on theoretical analysis.Experimental investigations were carried out to determine the acoustics of CVS60D.Corner vortex shedding is generated at the backward facing step which is designed similar to the geometry of the motor with finocyl propellant after the burnout of its fins.A pintle was used to modify the velocity in the duct to change the frequency of vortex shedding.It is found that large amplitude pressure oscillation occurs when the pintle moves to a range of specific position,which indicates that the frequency of vortex shedding is close to one order of acoustic modes of combustion chamber.The amplitude of pressure oscillation changes as the pintle moves.

Small Scale Field Experiment on Breaking Wave Pressure on Vertical Breakwaters

A small scale field experiment (SSFE) was performed on vertical breakwaters in the surf zone. The following are some of the findings. Wind seas may yield breaking wave pressure notwithstanding some large deepwater wave steepness, and small elevation of the wall above the mean water level. Caisson breakwaters can withstand some exceptionally high impulsive force peaks (even twice the weight in still water);whereas, with the same sea state and weight, a breakwater composed of layers of solid concrete blocks is destroyed.

The Research Status and Major Problems of High Temperature and High Pressure Experiment on Petrogenesis and mineralization

1 Introduction In contrast,1experimental geochemistry is a young subject,but in recent years,the research on experiment of high temperature and high pressure has become an important branch in the parallel subjects of traditional mineralogy,petrology,geochemistry and geophysics.It is not only an important and essential way and window to understand geological processes in depth and geological