Influence of Matrix Attachment Regions from Maize on Transgene Expression Level in Tobacco

The effect of matrix attachment regions (MARs) on foreign gene expression in transgenic plants was studied, The beta-glucuronidase (GUS) gene (uidA) was flanked by the MARs isolated from the genome of maize to form plant expression vector. The vectors with and without MARs were transferred into tobacco ( Nicotiana tabacum L.) through Agrobacterium-mediated transformation procedure. GUS activity assays indicated that MARs could increase expression level of uidA gene. The mean GUS activity could be increased twofold as compared to that of transformants without MARs, and the highest GUS activity of transformant could reach tenfold. The correspondence between GUS activity and mRNA accumulation was positive and indicated that MARs could improve transcription of foreign gene.

A complete compilation of matrix metalloproteinase expression in human malignant gliomas

Glioblastomas are characterized by an aggressive local growth pattern, a marked degree of invasiveness and poor prognosis. Tumor invasiveness is facilitated by the increased activity of proteolytic enzymes which are involved in destruction of the extracellular matrix of the surrounding healthy brain tissue. Elevated levels of matrix metalloproteinases(MMPs) were found in glioblastoma(GBM) cell-lines, as well as in GBM biopsies as compared with low-grade astrocytoma(LGA) and normal brain samples, indicating a role in malignant progression. A careful review of the available literature revealed that both the expression and role of several of the 23 human MMP proteins is controversely discussed and for some there are no data available at all. We therefore screened a panel of 15 LGA and 15 GBM biopsy samples for those MMPs for which there is either no, very limited or even contradictory dataavailable. Hence, this is the first complete compilation of the expression pattern of all 23 human MMPs in astrocytic tumors. This study will support a better understanding of the specific expression patterns and interaction of proteolytic enzymes in malignant human glioma and may provide additional starting points for targeted patient therapy.

Matrix metalloproteinase gene expressions might be oxidative stress targets in gastric cancer cell lines

Oxidative stress is linked to increased risk of gastric cancer and matrix metalloproteinases （MMPs） are important in the invasion and metastasis of gastric cancer. We aimed to analyze the effect of the accumulation of oxidative stress in the gastric cancer MKN-45 and 23132/87 cells following hydrogen peroxide （H202） exposure on the expression patterns of MMP-1, MMP-3, MMP-7, MMP-9, MMP-10, MMP- 1 1, MMP- 12, MMP-14, MMP- 15, MMP- 17, MMP-23, MMP-28, and β-catenin genes. Methods： The mRNA transcripts in the cells were determined by RT-PCR. Following H202 exposure, oxidative stress in the viable cells was analyzed by 2＇,7＇-dichlorofluorescein diaeetate （DCFH-DA）. Caffeie acid phenethyl ester （CAPE） was used to eliminate oxidative stress and the consequence of H2O2 exposure and its removal on the expressions of the genes were evaluated by quantitative real-time PCR. Results： The expressions of MMP-1, MMP-7, MMP-14, MMP-15, MMP-17 and β-catenin in MKN-45 cells and only the expression of MMP-15 in 23132/87 cells were increased. Removal of the oxidative stress resulted in decrease in the expressions of MMP genes of which the expressions were increased after H202 exposure. β-catenin, a transcription factor for many genes including MMPs, also displayed decreased levels of expression in both of the cell lines following CAPE treatment. Conclusions： Our data suggest that there is a remarkable link between the accumulation of oxidative stress and the increased expressions of MMP genes in the gastric cancer cells and MMPs should be considered as potential targets of therapy in gastric cancers due to its continuous exposure to oxidative stress.

Expression of Matrix Metalloproteinase-9 mRNA in Osteoporotic Bone Tissues

Matrix metalloproteinases (MMPs), a sort of irnportant enzymes involved in extracellular matrix metabolism, play critical r0Ies in the process of tissues remodeling, wound healing and metastasis of tumors. Dot blot and in situ hybridization were used in this study to detect the expression and localization of MMP- 9, an important proteolytic enzyme implicated in bone resorption, in bone tissues. The results showed that the level of MMP-9 mRNA expression in osteoporotic bone tissues was significantly higher than that in normal control group and the cell types that expressed MMP-9 mRNA incIuded mono- and multi-nuclear osteoclasts and some lining cells on the surface of bone matrix. It was suggested that MMP-9 play a key role in the development of bone loss in osteoporosis.

Altered expression of nuclear matrix proteins in etoposide induced apoptosis in HL-60 cells

The events of cell death and the expression of nuclear matrix protein (NMP) have been investigated in a promyelocytic leukemic cell line HL-60 induced with etoposide. By means of TUNEL assay, the nuclei displayed a characteristic morphology change, and the amount of apoptotic cells increased early and reached maximun about 39% after treatment with etoposide for 2 h. Nucleosomal DNA fragmentation was observed after treatment for 4 h. The morphological change of HL-60 cells, thus, occurred earlier than the appearance of DNA ladder. Total nuclear matrix proteins were analyzed by 2-dimensional gel electrophoresis. Differential expression of 59 nuclear matrix proteins was found in 4 h etoposide treated cells. Western blotting was then performed on three nuclear matrix acssociated proteins, PML, HSC70 and NuMA. The expression of the suppressor PML protein and heat shock protein HSC70 were significantly upregulated after etoposide treatment, while NuMA, a nuclear mitotic apparatus protein, was down regulated. These results demonstrate that significant biochemical alterations in nuclear matrix proteins take place during the apoptotic process.

ADHESION INDUCES MATRIX METALLOPROTEINASE-9 GENE EXPRESSION IN OVARIAN CANCER CELLS

Objective: To investigate the expression of matrix metalloproteinase-9 (MMP-9) gene in cancer cells induced by adhesion with fibronectin and the underlying mechanism of cell invasion. Methods: Following adhesion of ovarian cancer cells A2780 to fibronectin, MMP mRNA expression was assayed by using reverse transcription-polymerase chain reaction (RT-PCR). MMP-9 promoter was cloned from genomic DNA of HT1080 cells with PCR. The MMP-9-pGL2 reporter gene vector was constructed and then transiently transfected into A2780 cells. Results: Adhesion could induce the expression of MMP-9 gene in A2780 cells, but did not affect longer the expression of MMP-2 or TIMP-1 gene. The induction was enhanced with longer adhesion time. When the transfected cells were allowed to adhere and spread on FN-coated surface, the promoter activity of MMP-9 gene was also enhanced dramatically. Conclusion: adhesion of cells with ECM may stimulate the expression of MMP-9 gene through stimulating the promoter activity, thereby enhancing cancer cell invasion and metastasis.

Matrix Expression of the Orthogonal Wavelet (Packets) Transform

Matrix expression of finite orthogonal wavelet transform of finite impulse response signal is more valuable for theoretical analysis and understanding. However, clear deduction for matrix expression has not been provided yet. In this paper, the formulation to generate the re-lated matrix is put forward and the theorem on the orthogonality of this matrix proved. This effort deploys a basis for more deeper and wider applications in chemical processes. *

Improvement of foreign gene expression in transgenic rice(Oryza sativa L.)by matrix attachment regions(MARs)

Matrix attachment regions or matrix associated regions (MARs) were special DNA sequences in chromatin of eukaryotic cells that tightly associated with the nuclear matrix or scaffold in vitro after a combination of nuclease digestion and extraction. They were also called scaffold attachment regions(SARs) . It was found that MARs could improve the expression level and the stability of foreign genes in transgenic plants. The reason might be that a transgene flanked by MARs was transmitted into the plant cells, the MARs would attach the nuclear

A STUDY ON THE CARTOGRAPHIC FUZZY MATRIX MODEL AND MATHEMATICAL EXPRESSIONS OF CARTOGRAPHIC TERMS

On the basis of the research of the cartographic modelsmade by the authors some years ago,this paper further studies theestablishment of cartographic fuzzy matrix to make the concepts incartography e.g.,selection,deletion,mapping generalization andimage tranformation,into some precise mathematical expressions.

电子传递链对阿尔兹海默病的影响分析

阿尔兹海默病(Alzheimer’s disease,AD)是一种神经退行性疾病.临床上以记忆障碍、视空间技能损害、执行功能障碍以及人格行为改变等全面性痴呆表现为特征.AD致病源现今不明确,其中有毒物质损害使得脑内区域性能量代谢减退是AD发生的病因特征之一,而电子传递链(ETC)在能量代谢中扮演着重要角色.通过差异分析(LIMMA)及加权基因共表达网络(WGCNA)构建获得聚类基因,并对聚类后基因进行生理分析(GO)、通路分析(KEGG)后锁定ETC,以及对其进行表达量分析及相关系数矩阵的构建,并结合临床指标(MMSE)解释说明,最终验证了ETC的异常对阿尔兹海默病存在影响的事实.

血清MMP-9、sTREM-1对输尿管结石患者术后泌尿系统感染的预测价值

目的探讨血清基质金属蛋白酶9(MMP-9)、可溶性髓系细胞触发受体-1(sTREM-1)对输尿管结石患者术后泌尿系统感染(UTI)的预测价值。方法选取2021年10月至2022年10月在该院泌尿外科收治的输尿管结石患者中术后发生UTI的68例患者(UTI组)和未发生UTI的68例患者(非UTI组)作为研究对象。采用酶联免疫吸附试验(ELISA)检测血清MMP-9、sTREM-1水平。采用Spearman法分析UTI组血清MMP-9、sTREM-1水平与临床资料的相关性,受试者工作特征曲线分析血清MMP-9、sTREM-1水平对输尿管结石患者术后UTI的预测价值,以及多因素Logistic回归分析输尿管结石患者术后UTI发生的影响因素。结果与非UTI组相比,UTI组血清MMP-9、sTREM-1水平显著升高,差异有统计学意义(P<0.05)。相关性分析发现,UTI组患者血清MMP-9和sTREM-1水平呈正相关(r=0.585,P<0.001)。二者联合预测输尿管结石患者术后UTI的曲线下面积(AUC)为0.961(95%CI:0.934~0.988),其灵敏度、特异度分别为73.36%和85.68%,二者联合预测的AUC高于MMP-9和sTREM-1单独预测的AUC(Z=25.420,P<0.001;Z=21.531,P<0.001)。MMP-9、sTREM-1水平是输尿管结石患者术后UTI发生的影响因素(P<0.05)。结论输尿管结石术后UTI患者血清MMP-9、sTREM-1水平升高,二者水平检测对输尿管结石患者术后UTI的发生具有重要预测价值。

统一框架的增强深度子空间聚类方法

深度子空间聚类是一种处理高维数据聚类任务的有效方法。然而,现有的深度子空间聚类方法通常将自表示学习和指标学习作为两个独立的过程,导致在处理具有挑战性的数据时,固定的自表示矩阵会导致次优的聚类结果;另外,自表示矩阵的质量对聚类结果的准确性至关重要。针对上述问题,提出一种统一框架的增强深度子空间聚类方法。首先,通过将特征学习、自表示学习和指标学习集成在一起同时优化所有参数,根据数据的特征动态地学习自表示矩阵,确保准确地捕捉数据特征;其次,为了提高自表示学习的效果,提出类原型伪标签学习,为特征学习和指标学习提供自监督信息,进而促进自表示学习;最后,为了增强嵌入表示的判别能力,引入正交性约束帮助实现自表示属性。实验结果表明,与AASSC(Adaptive Attribute and Structure Subspace Clustering network)相比,所提方法在MNIST、UMIST、COIL20数据集上的聚类准确率分别提升了1.84、0.49、0.34个百分点。可见,所提方法提高了自表示矩阵学习的准确性,聚类效果更好。

Single-cell manipulation by two-dimensional micropatterning

Cells are highly sensitive to their geometrical and mechanical microenvironment that directly regulate cell shape,cytoskeleton and organelle,as well as the nucleus morphology and genetic expression.The emerging two-dimensional micropatterning techniques offer powerful tools to construct controllable and well-organized microenvironment for single-cell level investigations with qualitative analysis,cellular standardization,and in vivo environment mimicking.Here,we provide an overview of the basic principle and characteristics of the two most widely-used micropatterning techniques,including photolithographic micropatterning and soft lithography micropatterning.Moreover,we summarize the application of micropatterning technique in controlling cytoskeleton,cell migration,nucleus and gene expression,as well as intercellular communication.

RECK、MMP-2、bcl-2蛋白在牙龈瘤患儿牙龈组织中的表达分析

目的 探讨RECK、基质金属蛋白酶-2(MMP-2)、B淋巴细胞瘤-2基因(bcl-2)蛋白在牙龈瘤患儿牙龈组织中的表达情况。方法 选取80例牙龈瘤患儿作为研究对象,所有患儿均行手术治疗,采集肿瘤组织及肿瘤旁健康牙龈组织,采用免疫组化SP法检测样本内RECK、MMP-2、bcl-2蛋白表达情况,比较肿瘤组织及健康牙龈组织中上述表达情况;并随访1年,分析RECK、MMP-2、bcl-2蛋白表达与其复发的关系。结果 肿瘤组织中RECK阳性率为36.25%,低于健康牙龈组织的77.50%,MMP-2阳性率、bcl-2蛋白阳性率分别为73.75%、67.50%,高于对照组的18.75%、22.50%,差异有统计学意义(P<0.05);80例患儿术后随访1年,共出现33例复发,复发率为41.25%(33/80);复发组RECK阳性率为15.15%,低于未复发组的51.06%,MMP-2阳性率、bcl-2蛋白阳性率为90.91%、93.94%,高于未复发组的61.70%、48.94%,差异有统计学意义(P<0.05)。结论 RECK、MMP-2、bcl-2蛋白在牙龈瘤患儿牙龈组织内存在不同表达,RECK阳性表达率低,MMP-2、bcl-2蛋白表达偏高,其表达与复发存在密切关系,可为完善早期治疗工作提供一定指导。

广播总控矩阵调度监控系统的设计方案研究

总控矩阵作为广播电视台信号实时调度的关键系统,在融媒体信息汇聚、分发、共享等信息交互环节发挥了重要作用。为了更好地满足融媒体信息传播的实际需求,提出一种新的广播总控矩阵调度监控系统的设计方案,对传统音视频类广播总控系统进行了智能化升级,以更好地满足4K/8K高清视频信号传播的需要。

混合网络攻击下马尔可夫跳变神经网络的安全容错同步控制

针对一类混合网络攻击下马尔可夫跳变神经网络安全容错同步控制问题,提出1种确保神经网络主从系统安全容错同步控制器的设计方法。利用2个独立的伯努利分布对随机发生的欺骗攻击和拒绝服务(DoS)攻击进行建模,将马尔可夫跳变神经网络和混合网络攻击置于同一框架中,考虑到执行器可能发生的失效故障情况,设计1种容错控制器;引入1种自由矩阵方法,构建李雅普诺夫(Lyapunov)函数,采用线性矩阵不等式分析方法和特殊的积分不等式放缩技术,推导出能够保证同步误H∞差系统随机均方稳定且满足规定的性能指标的充分条件;进一步使用1种有效的解耦方法对矩阵的耦合项进行分离解耦,运用Matlab中的LMI工具箱得到控制器参数,最后通过数值算例说明所提方法的可行性和有效性。结果表明:当执行器故障失效60%~80%时,以及遭受欺骗攻击概率为80%和DoS攻击概率为50%的混合网络攻击时,所提方法仍然能实现神经网络主从系统的安全性和同步性。

不同程度冠脉病变冠心病患者血清亲环素A、可溶性生长刺激表达基因2蛋白、基质金属蛋白酶-29水平变化及其诊断价值分析

目的:分析不同程度冠脉病变冠心病患者血清亲环素A(CyPA)、可溶性生长刺激表达基因2蛋白(sST2)、基质金属蛋白酶-29(MMP-29)水平变化及其诊断价值。方法:收集60例冠心病患者作为冠心病组,另在同一时期内的体检人员中收集50例健康者作为对照组。其中冠心病组经冠脉造影检查后,根据病变类型分为三组,即不稳定心绞痛、稳定性心绞痛以及急性心肌梗死组;根据病变支数分为三组,即单支病变、双支病变以及三支病变组;根据Gensini评分分为三组,即轻度病变、中度病变以及重度病变组。比较观察组与对照组血清CyPA、sST2、MMP-29水平;比较不同疾病类型患者血清CyPA、sST2、MMP-29水平;比较不同病变支数患者血清CyPA、sST2、MMP-29水平;比较不同病变程度患者血清CyPA、sST2、MMP-29水平;分析冠心病患者血清CyPA、sST2、MMP-29水平与病变程度的相关性及诊断价值。结果:观察组血清CyPA、sST2、MMP-29水平高于对照组(均P<0.05);三组间血清CyPA、sST2、MMP-29水平比较差异有统计学意义,且与稳定性心绞痛组比较,不稳定性心绞痛组、急性心肌梗死组更高,而急性心肌梗死组比较不稳定性心较痛组也更高(均P<0.05);三组间血清CyPA、sST2、MMP-29水平比较差异有统计学意义,且双支病变组、三支病变组比较单支病变组更高,而三支病变组比较双支病变组也更高(均P<0.05);三组间血清CyPA、sST2、MMP-29水平比较差异有统计学意义,且与轻度病变组比较,中度病变组、重度病变组更高,而与中度病变组比较,重度病变组更高(均P<0.05);经Spearman相关性分析显示,冠心病患者血清CyPA、sST2、MMP-29检测值与其病变程度具有正相关关系(均P<0.05);经ROC曲线分析,血清CyPA诊断冠心病的曲线下面积(AUC)、特异度高于sST2和MMP-29(均P<0.05)。结论:冠心病患者血清CyPA、sST2、MMP-29水平越高,冠脉病变程度越严重,CyPA、sST2、MMP-29可作为冠心病病情诊断及评估的指标之一。

基于非负矩阵分解和Normal_Matrix的肿瘤基因分类

文中提出了一种结合非负矩阵分解和Normal_Matrix谱分解技术的肿瘤基因分类方法.其分类过程首先是利用fdr_test记分准则粗略除去噪声基因以实现基因表达谱数据的初步降维,进而运用非负矩阵分解萃取基因间的综合属性,通过综合属性构造样本间的Normal_Matrix并对其进行奇异值分解获取表征样本类别属性的谱分量实现肿瘤类型的分类识别.采用三组具有代表性的肿瘤基因表达谱数据进行实验,通过与其他方法的对比,其结果证明了文中方法的可行性和有效性.

Expression Optimization and Characterization of the Catalytic Domain of Human MT3-MMP

Matrix metalloproteinases（MMPs） are a family of proteases that are required for many biological processes and are also elevated in many pathological conditions. MMP inhibitors （MMPIs） may therefore be useful as therapeutic agents in treating a number of diseases including cancer, cardiovascular diseases and arthritis. Attempts have been made to develop MMPIs. Recombinant MMPs have been used to screening MMPs in vitro assays. In this work, we report the expression of MMP-16 in E. coli and the characterization of the recombinant MMP-16 with a commonly used MMP substrate DQ-gelatin.

Expression of strain tensor in orthogonal curvilinear coordinates

Based on an analysis of connotation and extension of the concept of the orthogonal curvilinear coordinates, we have deduced a platform of strain tensor expression of Cartesian coordinates, which turns out to be a function of Lame coefficient and unit vector. By using transform matrix between Cartesian coordinates and orthogonal eurvilinear coordinates, we have deduced a mathematical expression for correcting displacement vector differential in orthogonal curvilinear coordinates, and given a general expression of strain tensor in orthogonal curvilinear coordinates.