Objective:To clarify the effect of endurance training on the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tissues of mice after exhaustive exercise.Methods:A total of 45 male C57BL/6 mice were randoml...Objective:To clarify the effect of endurance training on the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tissues of mice after exhaustive exercise.Methods:A total of 45 male C57BL/6 mice were randomly divided into control(C),low-strength endurance training(LSET)and high-strength endurance training(HSET)groups(n=15).The mice in the control group were not conducted to platform training.The mice in the LSET and HSET groups were conducted to platform training at 30%and 60%of exhaustive exercise once a day for 5 days a week,respectively.The exhaustion exercise was performed after 5 weeks of platform training.Total RNA was extracted from myocardial tissues,and the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tissues was analyzed using Illimina transcriptome sequencing.Results:The distance and time of exhaustive exercise were longer in the LSET and HSET groups than in the control group,and the distance and time of exhaustive exercise were longer in the HSET group than in the LSET group(P<0.05).A total of 54 differentially expressed circRNAs(28 down-regulated and 26 up-regulated),7 differentially expressed lncRNAs(all down-regulated),3 differentially expressed miRNAs(1 down-regulated and 2 up-regulated)and 99 differentially expressed mRNAs(81 down-regulated and 18 up-regulated)were identified by transcriptome sequencing(P<0.05).Interaction network analysis revealed that ENSMUSG00000113041,MSTRG.79740,mmu-miR-374c-5p,18 down-regulated mRNAs and 3 up-regulated mRNAs formed a regulatory network.GO functional analysis revealed that the differentially expressed mRNAs were mainly enriched in primary metabolic processes and macromolecular synthesis and metabolic processes.KEGG pathway analysis revealed that the differentially expressed mRNAs were mainly enriched in complement and coagulation cascade pathways,estrogen signaling pathway and glucagon signaling pathway.Conclusion:Endurance training could alter the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tissues of mice after exhaustive exercise,and these differentially expressed RNAs form a regulatory network that affects cardiomyocyte synthesis and metabolism and thus participates in the regulation of myocardial injury.展开更多
In a recent issue of the Journal of Sport and Health Science,García-Pinillos et al.~1 reviewed evidence on the effects of highintensity intermittent training(interval training)(HIIT)on muscular and performance ad...In a recent issue of the Journal of Sport and Health Science,García-Pinillos et al.~1 reviewed evidence on the effects of highintensity intermittent training(interval training)(HIIT)on muscular and performance adaptations in recreational runners.展开更多
To handle the handover challenge in Express Train Access Networks(ETAN).mobility fading effects in high speed railway environments should be addressed first.Based on the investigation of fading effects in this paper,w...To handle the handover challenge in Express Train Access Networks(ETAN).mobility fading effects in high speed railway environments should be addressed first.Based on the investigation of fading effects in this paper,we obtain two theoretical bounds:HOTiming upper bound and HO-Margin lower bound,which are helpful guidelines to study the handover challenge today and in the future.Then,we apply them to analyze performance of conventional handover technologies and our proposal in ETAN.This follow-up theory analyses and simulation experiment results demonstrate that the proposed handover solution can minimize handover time up to 4ms(which is the fastest one so far),and reduce HO-Margin to 0.16 dB at a train speed of 350km/h.展开更多
The impact of international textile trade disputes on foreign direct investments in this field is rather clear, for the first half of 2006, with only 9.48 percent of growth in actual FDI value and its growth margin fe...The impact of international textile trade disputes on foreign direct investments in this field is rather clear, for the first half of 2006, with only 9.48 percent of growth in actual FDI value and its growth margin fell by 29.51 percentage points.展开更多
基金The Medical and Health Science and Technology Development Planning Project of Shandong Province(202103011061)。
文摘Objective:To clarify the effect of endurance training on the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tissues of mice after exhaustive exercise.Methods:A total of 45 male C57BL/6 mice were randomly divided into control(C),low-strength endurance training(LSET)and high-strength endurance training(HSET)groups(n=15).The mice in the control group were not conducted to platform training.The mice in the LSET and HSET groups were conducted to platform training at 30%and 60%of exhaustive exercise once a day for 5 days a week,respectively.The exhaustion exercise was performed after 5 weeks of platform training.Total RNA was extracted from myocardial tissues,and the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tissues was analyzed using Illimina transcriptome sequencing.Results:The distance and time of exhaustive exercise were longer in the LSET and HSET groups than in the control group,and the distance and time of exhaustive exercise were longer in the HSET group than in the LSET group(P<0.05).A total of 54 differentially expressed circRNAs(28 down-regulated and 26 up-regulated),7 differentially expressed lncRNAs(all down-regulated),3 differentially expressed miRNAs(1 down-regulated and 2 up-regulated)and 99 differentially expressed mRNAs(81 down-regulated and 18 up-regulated)were identified by transcriptome sequencing(P<0.05).Interaction network analysis revealed that ENSMUSG00000113041,MSTRG.79740,mmu-miR-374c-5p,18 down-regulated mRNAs and 3 up-regulated mRNAs formed a regulatory network.GO functional analysis revealed that the differentially expressed mRNAs were mainly enriched in primary metabolic processes and macromolecular synthesis and metabolic processes.KEGG pathway analysis revealed that the differentially expressed mRNAs were mainly enriched in complement and coagulation cascade pathways,estrogen signaling pathway and glucagon signaling pathway.Conclusion:Endurance training could alter the expression profile of circRNA-lncRNA-miRNA-mRNA in myocardial tissues of mice after exhaustive exercise,and these differentially expressed RNAs form a regulatory network that affects cardiomyocyte synthesis and metabolism and thus participates in the regulation of myocardial injury.
文摘In a recent issue of the Journal of Sport and Health Science,García-Pinillos et al.~1 reviewed evidence on the effects of highintensity intermittent training(interval training)(HIIT)on muscular and performance adaptations in recreational runners.
基金supported by the National Basic Research Program of China (973 Program)(No.2012CB315606 and 2010CB328201)
文摘To handle the handover challenge in Express Train Access Networks(ETAN).mobility fading effects in high speed railway environments should be addressed first.Based on the investigation of fading effects in this paper,we obtain two theoretical bounds:HOTiming upper bound and HO-Margin lower bound,which are helpful guidelines to study the handover challenge today and in the future.Then,we apply them to analyze performance of conventional handover technologies and our proposal in ETAN.This follow-up theory analyses and simulation experiment results demonstrate that the proposed handover solution can minimize handover time up to 4ms(which is the fastest one so far),and reduce HO-Margin to 0.16 dB at a train speed of 350km/h.
文摘The impact of international textile trade disputes on foreign direct investments in this field is rather clear, for the first half of 2006, with only 9.48 percent of growth in actual FDI value and its growth margin fell by 29.51 percentage points.
