The Analysis of Deficiency in College-student English Oral Expression Ability and Strategies for Breaking Barriers

Referring to the questionnaire surveys and literature materials, this paper describes the current situation of college students＇ English oral expression ability, the reasons for lack of such ability and possible solutions. This research used a questionnaire survey to collect data from 146 college students from different grades, majors, universities and areas. The result shows that the deficiency in college students＇ English oral expression ability can be explained on two levels, spiritual level and oral level. Spiritual level shows that college students don＇t always have thoughts while others are communicating with them in English; oral level includes two aspects, one is that they always have no idea of how to express their thoughts well and the other is that they fail to always express themselves clearly and exactly. It is suggested that a good language environment is necessary for students to practice English pronunciation, understand culture from English speaking countries, and enhance listening skill to overcome language anxiety in communication.

Construction of Recombinant Retroviral Vector Containing HIV-1 Tat Gene and Functional Detection of Expressed Tat in Target Cells

Objective: To construct recombinant retroviral vector containing HIV-1 Tatgene and evaluate the junction of the expressed Tat in target cells. Methods: HIV-1 Tat_(101) genewas recovered from pEV plasmid by Hind Ⅲ digestion and cloned into expression plasmid LZESpBMN-Z toconstruct recombinant retroviral expression plasmid named LZRS-Tat_(101). Using the method ofcalcium phosphate, the construct of LZRS-Tat_(101) was then transfected into packaging cell linesPhoenix (ΦNX) which contained env and gal genes encoding structural proteins and pol gene codingfor 3 enzymes ( reverse transcriptase, protease and integrate) essential for retroviral integrationand replication . The stable transfected cell lines was obtained using puromycin to screen for morethan 3 days. Then, immunohistochemical (IHC ) staining was carried out to detect the expressionlevel of Tat_(101) protein in both transiently and stably trancfected ΦNX, respectively. Thesupematants containing recombinant virus collected from transient and stable transfected cells wereemployed to infect 293 cells, respectively, and the expressed Tat in 293 cells was tested by Westernblot. Meantime, the supematants of infected 293 cells was further added to HL3T1 cells which wereHela cell lines containing an HIV-1-LTR/CAT reporter construct to establish a co-culture system.After co-culture for 72 hours, the protein was extracted from HL3T1 cells and used for CAT activityassay. Results: After LZRS- Tat_(101) was transfected into ΦNX, the amount of expressed Tat intransient transfection cells was significantly higher than that in stable transfection cells; Tatcould be detected not only in 293 cells but also in the supematants from 293 cells culture, and Tatin the supematants could activate HIV-1 LTR promoter in HL3T1, resulting in high 'expression of CATlocated at the downstream of LTR. Conclusion: The construct of recombinant retrovirus LZRS-Tat_(101) could express Tat protein in target cells and the expressed Tat was functionally activeand can really exhibit the ability to activate transcription.

Landscape Furniture's Expression of Regional Culture

Regional culture shows the personality of a city, the expression of regional culture in urban landscapes is the focus of modern urban landscape construction. By elaborating connotations of landscape furniture and regional culture, the ways of refining cultural connotations of city history, and expressing regional cultures in landscape furniture were explored to provide references for the expression of regional cultures in urban landscapes.

The expression of histone deacetylases and the regenerative abilities of spinal-projecting neurons after injury

Epigenetic control of regeneration after spinal cord injury： Com- plete spinal cord injury （SCI） in humans and other mammals leads to irreversible paralysis below the level of injury, due to failure of axonal regeneration in the central nervous system （CNS）. Previous work has shown that successful axon regeneration is dependent upon transcription of a large number of regeneration-associated genes （RAGs） and transcription factors （TFs） （Van Kesteren et al., 2011）. A prominent theory in the field of axon regeneration is that the large differences in regenerative potential between peripheral nervous system （PNS） neurons, which regenerate well, and CNS neurons, which do not, reflect differences in intrinsic transcriptional net- works, rather than individual genes （Van Kesteren et al., 2011）.

Effects of melatonin on learning abilities, cholinergic fibers and nitric oxide synthase expression in rat cerebral cortex

BACKGROUND: Melatonin is a kind of hormones derived from pineal gland. Recent researches demonstrate that melatonin is characterized by anti-oxidation, anti-senility and destroying free radicals. While, effect and pathogenesis of pineal gland on learning ability should be further studied. OBJECTIVE: To investigate the effects of pinealectomy on learning abiliy, distribution of cholinesterase and expression of neuronal nitric oxide synthase (nNOS) in cerebral cortex of rats and probe into the effect of melatonin on learning ability, central cholinergic system and nNOS expression. DESIGN: Randomized grouping design and animal study. SETTING: Department of Neurology, the 187 Hospital of Chinese PLA. MATERIALS: A total of 12 male SD rats, of normal learning ability testing with Y-tape maze, of clean grade, weighing 190-210 g, aged 6 weeks, were selected in this study. METHODS: The experiment was carried out in the Department of Neurology, Zhujiang Hospital from July 1997 to June 2000. All SD rats were divided into experimental group (n =6, pinealectomy) and control group (n =6, sham operation). Seven days later, rats in both two groups were continuously fed for 33 days. ① Learning ability test: The learning ability of rats was tested by trisection Y-type maze and figured as attempting times. ② Expression of acetylcholinesterase (AchE) was detected by enzyme histochemistry and nNOS was measured by SABC method. ③ Quantitative analysis of AchE fibers: AchE fibers density in unit area (surface density) was surveyed with Leica Diaplan microscope and Leica Quantimet 500+ image analytic apparatus and quantitative parameter was set up for AchE fibers covering density (μm2) per 374 693.656 μm2, moreover, the AchE fibers density was measured in Ⅱ-Ⅳ layers of motor and somatosensory cortex (showing three layers per field of vision at one time), in radiative, lacunaria and molecular layers of CA1, CA2 and CA3 areas, and in lamina multiforms of dentate gyrus. Three tissue slices were picked up randomly in the same part of each rat, together six tissue slices for nNOS expression and four near view (× 400) were selected in the parts of right neocortex, medial septal nucleus-diagonal band nucleus (SM-DB), corpus striatus and hippocampus to count nNOS-positive cells. MAIN OUTCOME MEASURES: Learning ability; distribution and quantitative analysis of AchE fibers; expression of nNOS in various cerebral areas. RESULTS: The twelve rats were all involved in the final analysis. ① Learning ability test: The learning abilities before operation in the experimental group [(14.67±4.97) times] were consistent with those in the control group [(14.33±4.32) times, P > 0.05], the learning abilities in the experimental group at 40 days after pinealectomy [(28.67±2.42) times] were obviously more than those before pinealectomy and those in the control group after operation [(13.83±8.33) times, P < 0.01]. ② Results of AchE-positive fibers density in cerebral cortex of rats: The AChE-positive fibers densities in motor and somatosensory cortex, CA1, CA2 and CA3 areas of hippocampus and in lamina multiforms of dentate gyrus in the experimental group were obviously lower than those in the control group [experimental group: (15 244±1 339), (14 764±1 391), (12 991±970), (15 077±1 020), (19 546±1 489), (19 337±1 378) μm2; control group: (21 001±1 021), (17 930±2 225), (17 260±1 342), (18 911±1 048), (24 108±1 671), (22 917±1 909) μm2, P < 0.01]. ③ Expression of nNOS in various cerebral areas: nNOS-positive cells in cerebral cortex of rats of the experimental group were more, furthermore the ones in somatosensory cortex were slightly more in motor cortex and the number (5.90±0.68) was more than that in the control group (3.68±0.39,P < 0.05). The nNOS-positive cells in SM-DB (16.21±2.03) were markedly more than those in the control group (9.32±1.05,P < 0.01). The nNOS-positive cells in hippocampus (4.27±0.75) and in corpus striatus (9.35±2.58) were not different with those in the control group (3.94±0.53, 8.96±2.31, P > 0.05). CONCLUSION: Decrease of melatonin due to pinealectomy of rats can result in learning disorder, which may be related to trauma of cholinergic neuron in cerebral cortex which were caused by nitric oxide neurotoxicity arose from the overexpression of nNOS in cerebral neocortex and SM-DB.

Changes of TIZ expression in epithelial ovarian cancer cells

Objective:To study the change of TIZ expression in epithelial ovarian cancer cells.Methods:HO8910 cells were transinfected with siRNA to inhibit the expression of TIZ.pcDNA3.1-TIZ vectors were combined to increase the TIZ expression level.The cell viabilily,colony forming efficiency and cycle distribution of HO8910,HO8910/NC,HO8910/pcDNA3.1-NC,HO8910/ TIZ-573 and HO8910/pcDNA3.l-TIZ were compared,and the invasion rate,migration rate and adhesion rate between 5 groups of cells were compared.Results:Compared with those of HO8910,HO8910/NC and HO8910/pcDNA3.1-NC,the cell viability,colony forming efficiency and cell cycle distribution of HO8910/ TIZ-573 were increased,while the indexes of HO8910/pcDNA3.1-NC were decreased with statistical significant difference(P<0.05).There was no statistical significant difference in the invasion rate,migration rate and adhesion rate between 5 groups of cells(P>0.05).Conclusions:The expression of TIZ can inhibit the proliferation of epithelial ovarian cancer cells.

Effects of using a computer-lab on oral English fluency amongst all Chinese students at WOU

The purpose of this study is to assess the effectiveness of using a computer-lab on oral English fluency amongst all Chinese students at Western Oregon University （WOU）. The participants are oversea Chinese graduate students who take undergraduate courses at WOU, and some of them regard English as their Second Language. The study adopts the experimental design, There are total of 60 Chinese students participating in the study. The results indicate that the use of a Computer-Lab significantly improved students＇ English oral expression ability.

新时代背景下环境类研究生学术表达与创新能力培养

随着环境问题日益凸显,人们越来越意识到新时代生态文明建设的重要性。环境类研究生教育旨在启发学生树立生态文明建设理念,系统地解决复杂环境问题,推动创新型国家建设。学术表达能力是研究生综合素质和创新能力的重要体现,该文分析目前环境类研究生培养过程中存在的问题,以及影响研究生学术表达的多种因素,着重阐述如何通过学术表达能力培养课程培养研究生的创新能力,以期培养兼具学术表达与创新能力的高素质人才。

基于异形同义现象的机器空间语义理解能力评测研究

不同的方位义词语可以用于表达相同的空间场景。文章考察了汉语空间表达“异形同义”现象的不同类型,并据此制作测试题,作为“异形同义判别”子任务,成为中文空间语义理解能力SpaCE评测基准的一个组成部分。针对大语言模型的评测结果显示,大语言模型在“异形同义判别”任务上与人类水平尚有较大差距,且机器在不同试题上的表现特点也与人类表现有所不同。从空间认知图式的角度讲,大语言模型基于语符分布学习到的人类语言知识,还没有转化为类人的空间认知图式理解能力。

参赛动机、团队关系强度对大学生创新创业能力影响的实证研究

提升大学生创新创业能力是创新创业教育的核心功能。大学生创新创业能力的发展受个人内部因素以及外部团队因素等多方面的影响。通过构建一个包含参赛动机、关系强度和创新创业能力的模型探讨团队关系强度在参赛动机和创新创业能力之间的中介作用,并根据对612位参与创新创业大赛大学生的问卷调研获取的数据,运用结构方程模型和Bootstrap方法来验证研究假设。研究结果表明,团队关系强度在参赛动机(包括社会交往动机、自我表达动机以及自我发展动机)和创新创业能力之间起中介作用。

幼小衔接视角下教育戏剧提升6-7岁儿童口语表达能力的干预研究

教育戏剧活动是一种集戏剧表达、戏剧创作、戏剧表演为一体的综合性主题教育活动,其包含的多种教育元素能够有效促进儿童的社会能力、语言能力、情感态度等多方面的发展。为探究应用教育戏剧的口语训练活动对提升6-7岁儿童口语表达能力的影响,开展针对性教育实验,对刚升入小学一年级的86名儿童进行为期12周的教育干预。结果表明,教育戏剧活动能在倾听、表达、应对等维度有效提升儿童口语表达能力。主要建议:支持多向互动,为儿童创设宽松自由的表演环境;推进知行合一,积极应用教育戏剧范式充盈活动;支持幼小衔接,绘本资源入戏贴近儿童心理需求。

创新创业活动对大学生创业就业能力的影响

为探究参与创新创业活动对大学生创业就业能力的影响,将创业就业能力划分为创新创业意识、创新能力、创业能力、创业管理能力四个维度,对某高校学生进行问卷调查,将从未参加过创新创业活动学生的问卷结果作为对照组,将参加过创新创业活动学生的问卷结果作为实验组,利用SPSS 19.0软件进行数据分析。研究结果显示,实验组各维度的表现均优于对照组,初步说明参与创新创业活动对大学生创业就业能力的提升具有积极作用,从二级维度来看,学生的改革意愿、市场开拓能力等方面还应进行针对性提升。

美术教育中色彩表现能力培养的教学策略探索

在美术学习的核心领域中,色彩表现能力是学生艺术素养的重要组成部分。通过培养学生的色彩表现能力,能够提升学生的审美能力、增强学生的创作能力,并且促进学生情感交流沟通,因此,学校应当积极分析当前美术教育当中存在的问题,进而制定有效的教学策略,通过组织色彩感知与观察训练、构建系统化的色彩知识体系、积极开展色彩实践教学、注重现代技术的应用与实践等,激发学生对色彩的兴趣与探索欲,提升其色彩感知力、理解力和表现力,从而为其未来的艺术创作打下坚实的基础。

长链非编码RNA 00941、微小RNA-145表达及转化生长因子β1/Smad通路相关因子在胃癌组织中的表达及意义

目的:探究长链非编码RNA00941(Linc00941)、微小RNA-145(miR-145)表达及转化生长因子β1/Smad(TGF-β1/Smad)通路相关因子在胃癌组织中的表达与意义。方法:选取收治的51例胃癌患者,手术治疗后经病理学检查确诊。收集其胃癌组织及其癌旁组织,比较其中Linc00941、miR-145及TGF-β1/Smad通路相关因子表达差异;提取患者的胃癌组织细胞进行组织培养,并对其进行Linc00941、miR-145基因的转染、沉默,分析其对胃癌细胞的增殖、迁移和侵袭能力的影响。结果:与癌旁组织比较较,胃癌组织患者的Linc00941及其信使核糖核酸(mRNA)相对表达水平升高,miR-145及其mRNA相对表达水平降低(均P<0.05)。胃癌组织中的TGF-β1、转化生长因子β受体Ⅱ(TGF-βRⅡ)及Smad2/3均较癌旁组织显著降低(均P<0.05)。NC组、Linc00941转染组及Linc00941沉默组三组间吸光值(A值)及凋亡率两两比较均存在统计学差异(均P<0.05),Linc00941沉默组在培养24、48、72 h时的A值低于NC组,凋亡率高于NC组;Linc00941转染组各时间的A值高于NC组,凋亡率低于NC组(均P<0.05)。与NC组比较较,Linc00941沉默组的细胞迁移数目及细胞侵袭数目均降低,Linc00941转染组均升高,组间两两比较差异存在统计学意义(均P<0.05)。与NC组比较较,miR-145转染组培养24、48、72 h的A值均降低,凋亡率升高;miR-145沉默组的A值升高,细胞凋亡率降低(均P<0.05)。与NC组比较较,miR-145转染组的细胞迁移数目及细胞侵袭数目均降低,miR-145沉默组均升高,组间两两比较差异存在统计学意义(均P<0.05)。结论:在胃癌组织中,Linc00941及其mRNA相对表达水平升高,miR-145及其mRNA相对表达水平降低,TGF-β1/Smad通路相关因子TGF-β1、TGF-βRⅡ及Smad2/3表达降低,且Linc00941、miR-145表达影响胃癌细胞的增殖、侵袭和迁移。

小学语文作业中色彩设计对学生创意表达能力的促进作用

在探讨小学语文教学的多维途径时,作业设计无疑是一个不容忽视的方面。传统的语文作业往往侧重于知识的积累与记忆,而忽视了对学生创意表达能力的培养。然而,随着教育理念的更新与发展,我们越来越认识到创意表达能力在学生全面发展中的重要性。在这一背景下,小学语文作业中的色彩设计成为了一个值得深入研究的课题。研究旨在探讨小学语文作业中色彩设计对学生创意表达能力的促进作用,以期为小学语文教学提供新的思路和方法。

初中美术课程中色彩对学生情感表达能力的影响研究

色彩可以触动人的情感,刺激人的情感表达,因此在初中美术课程中需要通过色彩提升学生的情感表达能力。文章主要在分析初中美术课程中色彩对学生情感表达能力的影响的基础上,提出了在初中美术课程中通过色彩提升学生情感表达能力的对策,旨在为学生的艺术成长和情感表达搭建一个更加广阔的平台,从而有效地促进学生的全面发展。

高职院校大学语文阅读教学策略探赜

阅读教学作为大学语文的重要组成部分,承载着培养学生人文素养、提升学生语言表达能力的重任。文章分析高职院校大学语文阅读教学的意义,探讨高职院校大学语文阅读教学面临的挑战,指出高职院校大学语文阅读教学的策略是实施分层教学、融合专业特色、巧用信息技术、强化实践教学以及完善评价体系等,以提高学生的阅读能力和素养,推动高职院校大学语文阅读教学向新的高度迈进。

音乐教学中文化与情感素养培养策略探索

音乐作为一种文化艺术表现形式,具有丰富的文化内涵和积极的情感价值。在音乐教学中,教师可借助其培养学生的文化素养和情感素养,积极提高学生的审美能力,塑造学生的健全人格。在具体实践中,可以培养学生的音乐欣赏能力、音乐表现能力、音乐创造力,引导学生了解音乐的历史和文化背景;引导学生感受音乐中的情感,带领学生参加音乐活动,提高学生的音乐感知能力。

学生数学语言精准化表达能力培养策略探研

数学语言包含符号和数据,是解读数学概念、数学原理、数学案例、数学实验的专业用语。教师对数学语言表达资源作筛选、整合,对数学语言表达活动作优化设计,对数学语言表达训练作深度探索,能为学生创造更多表达机会,让学生在实践中逐步提升语言表达的精准性,并获得更多学习启迪。教师应整合教学内容,借助实例论证,创新观察设计,组织实验活动,对接生活实际,引导学生对逻辑关系作梳理,对数形关系作解读,对等量关系作分析,促进学生数学思维的成长,拓宽学生的数学视野。

高校环境设计专业“设计综合表达”课程教学改革分析

“设计综合表达”是设计专业的进阶性课程,目前各大高校均有开设,其中,以环境设计、工业设计两大专业开设居多。文章主要从环境设计专业出发,结合专业特色,为环境设计专业学生设计课程并进行教学改革。“设计综合表达”课程是当下设计领域不断革新变化下的积极探索性课程,亦是对学生过往所学专业课程的理论知识与实践能力的整合与创新。通过教学创新性改革,拓宽学生设计视角,使其学会更加逻辑性、综合性地分析场地环境,理解设计综合表达的意义与价值,了解设计表达的多样性。