[Objectives]To investigate the effect of low temperature treatment on cotton leaves at the two-leaf stage.[Methods]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They w...[Objectives]To investigate the effect of low temperature treatment on cotton leaves at the two-leaf stage.[Methods]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They were treated at 25(CK)and 12℃(low temperature)for 0,3,6,12,24,48 and 72 h,respectively.Then,the changes in the contents of MDA,SS and Pro in the cotton leaves were analyzed.Based on the analysis results,RNA-seq verification was performed.[Results]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They were treated at 25(CK)and 12℃(low temperature)for 0,3,6,12,24,48 and 72 h,respectively.Then,the changes in the contents of MDA,SS and Pro in the cotton leaves were analyzed.Based on the analysis results,RNA-seq verification was performed.[Conclusions]These genes may play an important role in improving the cold resistance of cotton.展开更多
To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot （Seophthalmus maximus）, water temperature was lowered rapidly from 18 to 1℃. During the cooling proces...To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot （Seophthalmus maximus）, water temperature was lowered rapidly from 18 to 1℃. During the cooling process, three individuals were removed from culture tanks at 18, 13, 8, 5, 3, and 1℃. Blood samples and tissues were taken from each individual, hematological indices and HSP gene expression in tissues were measured. The red blood cell count, white blood cell count, and hemoglobin concentration decreased significantly （P〈0.05） as temperature decreased. Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased, whereas aspartic transaminase and γ-glutamyl transpeptidase activities displayed no obvious changes above 1℃ and lactate dehydrogenase activity increased first and then decreased. Blood urea nitrogen and uric acid levels were highest at 8℃, and creatinine concentration was highest at 3℃. The concentrations of plasma cortisol, cholesterol, and triglyceride all increased significantly （P〈0.05） as temperature decreased. The serum glucose concentration increased first and then decreased to the initial level. The HSP70 mRNA expression showed various patterns in different tissues, whereas HSP90 mRNA expression showed the same tendency in all tissues. Overall, these results indicate that temperature decreases in the range of 8 to 5℃ may induce a stress response in S. maximus and that temperature should be kept above 8℃ in the aquaculture setting to avoid damage to the fish.展开更多
Low temperature is one of the adversities threatening the growth and development and reduces the yield of rubber trees.However,molecular mechanisms toward rubber trees in response to low temperature are largely unclea...Low temperature is one of the adversities threatening the growth and development and reduces the yield of rubber trees.However,molecular mechanisms toward rubber trees in response to low temperature are largely unclear.In this study,7,159 and 7,600 differentially expressed genes(DEGs)were identified in‘Reyan 73397’rubber trees.Through GO analysis,the catalytic activity was the representative of the GO term in the only DEGs at the two studied temperatures(room temperature and 4°C,respectively),while KEGG analysis showed that carbon metabolism was the most important grouping under the comparison of these two temperatures.In addition,expression of 9 members of transcription factor MYB family genes were further verified by qRT-PCR,and MYB family genes may play important roles in the regulation of rubber trees under low temperature stress.This study provided a theoretical foundation for(1)revealing the molecular mechanisms of rubber trees in response to low temperature and(2)breeding of tolerant varieties of rubber trees.展开更多
Many proteins require assistance from molecular chaperones at various stages to attain correctly folded states and functional conformations during protein synthesis. In this study, the gene encoding T-complex polypept...Many proteins require assistance from molecular chaperones at various stages to attain correctly folded states and functional conformations during protein synthesis. In this study, the gene encoding T-complex polypeptide 1（TCP-1）, which belongs to the heat shock protein 60（HSP60） family, was isolated and characterized from the rice stem borer, Chilo suppressalis, by RACE and q PCR, respectively. The full-length c DNA of Tcp-1 was 2 144 bp and encoded a 1 635-bp ORF; the deduced translational product contained 545 amino acids with 5′-and 3′-UTRs and an isoelectric point of 5.29. Cluster analysis confirmed that the deduced amino acid sequence shared high identity（60–99%） with TCP-1 from other insects. To investigate Tcp-1 expression in response to abiotic stress, q PCR was used to analyze expression levels of Tcp-1 m RNA in C. suppressalis larvae exposed to temperatures ranging from –11 to 43°C. With respect to heat shock, Tcp-1 expression was higher than the control after a 2-h exposure to 30 and 36°C and declined at 39 and 43°C. Difference in Tcp-1 expression was observed at temperatures ranging from –11 to 27°C. q PCR analyses revealed that Tcp-1 expression was the highest in hindgut tissue as compared to heads, epidermis, fat body, foregut, midgut, and malpighian tubules. Our results indicated that Tcp-1 expression was differentially expressed in C. suppressalis tissues, and was impacted by temperature stress.展开更多
Catalase is an important antioxidant protein that can protect organisms against various forms of oxidative damage by eliminating hydrogen peroxide. In this study, the catalase c DNA of Paphia textile（Pt CAT） was clo...Catalase is an important antioxidant protein that can protect organisms against various forms of oxidative damage by eliminating hydrogen peroxide. In this study, the catalase c DNA of Paphia textile（Pt CAT） was cloned using RTPCR and rapid amplification of c DNA ends（RACE）. Pt CAT is 1 921 bp long and consists of a 5′-UTR of 50 bp, a 3′-UTR of 349 bp, and an ORF of 1 542 bp that encodes 513 amino acids with a molecular weight of 58.4 k D and an estimated isoelectric point of 8.2. Sequence alignment indicated that Pt CAT contained a highly conserved catalytic signature motif（^（61）FNRERIPERVVHAKGAG^（77））, a proximal heme-ligand signature sequence（^（352）RLFSYSDP^（359））, and three catalytic amino acid residues（H^（72）, N^（145）, and Y^（356））. Pt CAT also contains two putative N-glycosylation sites（^（34）NKT^（36） and ^（437）NFT^（439）） and a peroxisome-targeting signal（^（511）AQL^（513））. Furthermore, Pt CAT shares 53%–88% identity and 29%–89% similarity with other catalase amino acid sequences. Pt CAT m RNA was present in all tested organs, including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, but its expression was highest in the digestive gland. High-temperature-induced stress produced two expression patterns of Pt CAT m RNA： first, an initial up-regulation followed by a down-regulation in the heart, digestive gland, and gonad and, second, consistent down-regulation in all other organs. These results demonstrate that Pt CAT is a typical member of the catalase family and might be involved in the responses to harmful environmental factors.展开更多
Environmental conditions, including ambient temperature, play important roles in survival growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed gr...Environmental conditions, including ambient temperature, play important roles in survival growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study, we investigated the effect of low temperature on gene expression profiles inA.japonicus by suppression subtractive hybridization （SSH）. Genes encoding Ferritin, Lysozyme, Hsp70, gp96, and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profiles of these genes were investigated in different tissues （coelomocyte, respiratory tree, intestine, longitudinal muscle） after exposure to acute and mild temperature dropping treatments. The results show that （1） the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; （2） under the temperature dropping treatments, three types of transcriptional regulation patterns were observed： primary suppression followed by up-regulation at -2℃, suppressed expression throughout the two treatments, and more rarely an initial stimulation followed by suppression; and （3） gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were ,distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature （-2℃）.展开更多
低温逆境已成为限制全球小麦稳产丰产与优质的主要农业气象灾害之一。为客观全面地了解全球小麦低温逆境领域的研究现状和发展趋势,基于Web of Science核心数据库中2000-2020年发表的以“小麦”和“低温”为研究主题的相关文献为数据源...低温逆境已成为限制全球小麦稳产丰产与优质的主要农业气象灾害之一。为客观全面地了解全球小麦低温逆境领域的研究现状和发展趋势,基于Web of Science核心数据库中2000-2020年发表的以“小麦”和“低温”为研究主题的相关文献为数据源,利用CiteSpace可视化分析软件对检索到与主题密切相关的475篇文献从发文数量、发文国家、发文作者、研究机构、研究热点等方面进行统计分析。结果表明:自2000年以来,小麦低温逆境领域年发文数量总体呈波动上升趋势;以中国、美国和加拿大等国家为代表的农业大国均致力于该领域的研究;美国学者Skin⁃ner是该领域发文量最多的作者,与其他学者合作密切的有李向楠和Fowler;该领域文献多发表于《Molecular Genetics and Genomics》和《Annual Review of Plant Biology》等优质期刊上;俄罗斯科学院是该领域发文量最多的科研机构;耐低温小麦新品种培育、耐寒分子生物学研究以及提高小麦产质量等方面成为该领域的研究热点。展开更多
基金Supported by Integration and Demonstration Project of Simple and Efficient Cultivation Technology of Cotton(2020YFD1001000)Special Fund for Construction of Modern Agricultural Industrial Technology SystemProject of Hubei Agricultural Science and Technology Innovation Center(2019-620-000-06)
文摘[Objectives]To investigate the effect of low temperature treatment on cotton leaves at the two-leaf stage.[Methods]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They were treated at 25(CK)and 12℃(low temperature)for 0,3,6,12,24,48 and 72 h,respectively.Then,the changes in the contents of MDA,SS and Pro in the cotton leaves were analyzed.Based on the analysis results,RNA-seq verification was performed.[Results]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They were treated at 25(CK)and 12℃(low temperature)for 0,3,6,12,24,48 and 72 h,respectively.Then,the changes in the contents of MDA,SS and Pro in the cotton leaves were analyzed.Based on the analysis results,RNA-seq verification was performed.[Conclusions]These genes may play an important role in improving the cold resistance of cotton.
基金Supported by the Special Foundation for Youth Science and Technology of Qingdao(No.14-2-4-59-jch)the Science and Technology Development Planning of Shandong Province(No.2011GGC21007)the National Natural Science Foundation of China(No.41206144)
文摘To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot （Seophthalmus maximus）, water temperature was lowered rapidly from 18 to 1℃. During the cooling process, three individuals were removed from culture tanks at 18, 13, 8, 5, 3, and 1℃. Blood samples and tissues were taken from each individual, hematological indices and HSP gene expression in tissues were measured. The red blood cell count, white blood cell count, and hemoglobin concentration decreased significantly （P〈0.05） as temperature decreased. Enzyme activities of plasma alanine transaminase and creatine kinase increased as temperature decreased, whereas aspartic transaminase and γ-glutamyl transpeptidase activities displayed no obvious changes above 1℃ and lactate dehydrogenase activity increased first and then decreased. Blood urea nitrogen and uric acid levels were highest at 8℃, and creatinine concentration was highest at 3℃. The concentrations of plasma cortisol, cholesterol, and triglyceride all increased significantly （P〈0.05） as temperature decreased. The serum glucose concentration increased first and then decreased to the initial level. The HSP70 mRNA expression showed various patterns in different tissues, whereas HSP90 mRNA expression showed the same tendency in all tissues. Overall, these results indicate that temperature decreases in the range of 8 to 5℃ may induce a stress response in S. maximus and that temperature should be kept above 8℃ in the aquaculture setting to avoid damage to the fish.
基金supported by the following grants:the National Key R&D Program of China(2019YFD1000500)the National Natural Science Foundations of Shandong Province(ZR2020MC138)the Agricultural Variety Improvement Project of Shandong Province(2020LZGC007).
文摘Low temperature is one of the adversities threatening the growth and development and reduces the yield of rubber trees.However,molecular mechanisms toward rubber trees in response to low temperature are largely unclear.In this study,7,159 and 7,600 differentially expressed genes(DEGs)were identified in‘Reyan 73397’rubber trees.Through GO analysis,the catalytic activity was the representative of the GO term in the only DEGs at the two studied temperatures(room temperature and 4°C,respectively),while KEGG analysis showed that carbon metabolism was the most important grouping under the comparison of these two temperatures.In addition,expression of 9 members of transcription factor MYB family genes were further verified by qRT-PCR,and MYB family genes may play important roles in the regulation of rubber trees under low temperature stress.This study provided a theoretical foundation for(1)revealing the molecular mechanisms of rubber trees in response to low temperature and(2)breeding of tolerant varieties of rubber trees.
基金funded by the National Natural Science Foundation of China (31401733)the Incubation Study Project of Science and Technology of Fuyang Normal University, China (2014KJFH02)
文摘Many proteins require assistance from molecular chaperones at various stages to attain correctly folded states and functional conformations during protein synthesis. In this study, the gene encoding T-complex polypeptide 1（TCP-1）, which belongs to the heat shock protein 60（HSP60） family, was isolated and characterized from the rice stem borer, Chilo suppressalis, by RACE and q PCR, respectively. The full-length c DNA of Tcp-1 was 2 144 bp and encoded a 1 635-bp ORF; the deduced translational product contained 545 amino acids with 5′-and 3′-UTRs and an isoelectric point of 5.29. Cluster analysis confirmed that the deduced amino acid sequence shared high identity（60–99%） with TCP-1 from other insects. To investigate Tcp-1 expression in response to abiotic stress, q PCR was used to analyze expression levels of Tcp-1 m RNA in C. suppressalis larvae exposed to temperatures ranging from –11 to 43°C. With respect to heat shock, Tcp-1 expression was higher than the control after a 2-h exposure to 30 and 36°C and declined at 39 and 43°C. Difference in Tcp-1 expression was observed at temperatures ranging from –11 to 27°C. q PCR analyses revealed that Tcp-1 expression was the highest in hindgut tissue as compared to heads, epidermis, fat body, foregut, midgut, and malpighian tubules. Our results indicated that Tcp-1 expression was differentially expressed in C. suppressalis tissues, and was impacted by temperature stress.
基金The National Natural Science Foundation of China under contract No.31172397the New Century Excellent Talents of Fujian Province University under contract No.JA14167the Open Research Fund Program of Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment under contract No.Z814041
文摘Catalase is an important antioxidant protein that can protect organisms against various forms of oxidative damage by eliminating hydrogen peroxide. In this study, the catalase c DNA of Paphia textile（Pt CAT） was cloned using RTPCR and rapid amplification of c DNA ends（RACE）. Pt CAT is 1 921 bp long and consists of a 5′-UTR of 50 bp, a 3′-UTR of 349 bp, and an ORF of 1 542 bp that encodes 513 amino acids with a molecular weight of 58.4 k D and an estimated isoelectric point of 8.2. Sequence alignment indicated that Pt CAT contained a highly conserved catalytic signature motif（^（61）FNRERIPERVVHAKGAG^（77））, a proximal heme-ligand signature sequence（^（352）RLFSYSDP^（359））, and three catalytic amino acid residues（H^（72）, N^（145）, and Y^（356））. Pt CAT also contains two putative N-glycosylation sites（^（34）NKT^（36） and ^（437）NFT^（439）） and a peroxisome-targeting signal（^（511）AQL^（513））. Furthermore, Pt CAT shares 53%–88% identity and 29%–89% similarity with other catalase amino acid sequences. Pt CAT m RNA was present in all tested organs, including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, but its expression was highest in the digestive gland. High-temperature-induced stress produced two expression patterns of Pt CAT m RNA： first, an initial up-regulation followed by a down-regulation in the heart, digestive gland, and gonad and, second, consistent down-regulation in all other organs. These results demonstrate that Pt CAT is a typical member of the catalase family and might be involved in the responses to harmful environmental factors.
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A412)the National Natural Science Foundation of China(No.31072230)
文摘Environmental conditions, including ambient temperature, play important roles in survival growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study, we investigated the effect of low temperature on gene expression profiles inA.japonicus by suppression subtractive hybridization （SSH）. Genes encoding Ferritin, Lysozyme, Hsp70, gp96, and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profiles of these genes were investigated in different tissues （coelomocyte, respiratory tree, intestine, longitudinal muscle） after exposure to acute and mild temperature dropping treatments. The results show that （1） the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; （2） under the temperature dropping treatments, three types of transcriptional regulation patterns were observed： primary suppression followed by up-regulation at -2℃, suppressed expression throughout the two treatments, and more rarely an initial stimulation followed by suppression; and （3） gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were ,distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature （-2℃）.
文摘低温逆境已成为限制全球小麦稳产丰产与优质的主要农业气象灾害之一。为客观全面地了解全球小麦低温逆境领域的研究现状和发展趋势,基于Web of Science核心数据库中2000-2020年发表的以“小麦”和“低温”为研究主题的相关文献为数据源,利用CiteSpace可视化分析软件对检索到与主题密切相关的475篇文献从发文数量、发文国家、发文作者、研究机构、研究热点等方面进行统计分析。结果表明:自2000年以来,小麦低温逆境领域年发文数量总体呈波动上升趋势;以中国、美国和加拿大等国家为代表的农业大国均致力于该领域的研究;美国学者Skin⁃ner是该领域发文量最多的作者,与其他学者合作密切的有李向楠和Fowler;该领域文献多发表于《Molecular Genetics and Genomics》和《Annual Review of Plant Biology》等优质期刊上;俄罗斯科学院是该领域发文量最多的科研机构;耐低温小麦新品种培育、耐寒分子生物学研究以及提高小麦产质量等方面成为该领域的研究热点。
