Based on the Prototype Theory,the prototypical feature of advertisement is found to be the combination of three language functions:the informative function,the expressive function,and the vocative function.The adverti...Based on the Prototype Theory,the prototypical feature of advertisement is found to be the combination of three language functions:the informative function,the expressive function,and the vocative function.The advertisement translation means the adjustment of the informative function and the expressive function according to the differences between languages or cultures in order to maximize the vocative function.The faithful translation is the closest to the prototype of the source text but not necessarily the best translation.展开更多
The expression and function in growth and apoptosis of the renin-angiotensin system(RAS)was evaluated inhuman glioblastoma.Renin and angiotensinogen(AGT)mRNAs and proteins were found by in situ hybridisationand immuno...The expression and function in growth and apoptosis of the renin-angiotensin system(RAS)was evaluated inhuman glioblastoma.Renin and angiotensinogen(AGT)mRNAs and proteins were found by in situ hybridisationand immunohistochemistry in glioblastoma cells.Angiotensinogen was present in glioblastoma cystic fluids.Thus,human glioblastoma cells produce renin and AGT and secrete AGT.Human glioblastoma and glioblastoma cellsexpressed renin,AGT,renin receptor,AT(2)and/or AT(1)mRNAs and proteins determined by RT-PCR and/展开更多
In this paper, it is pointed that the general expression for the stress function of the plane problem in polar coordinates is incomplete. The problems of the curved bar with an arbitrary distributive load at the bound...In this paper, it is pointed that the general expression for the stress function of the plane problem in polar coordinates is incomplete. The problems of the curved bar with an arbitrary distributive load at the boundries can't he solved by this stress function. For this reason, we suggest two new stress functions and put them into the general expression. Then, the problems of the curved bar applied with an arbitrary distributive load at r=a,b boundaries can be solved. This is a new stress function including geometric boundary constants.展开更多
Fiber cell initiation is a complex process involving many pathways,including phytohormones and components for transcriptional and posttranscriptional regulation.Here we report expression
This paper investigates the transition function and the reachability conditions of finite automata by using a semitensor product of matrices, which is a new powerful matrix analysis tool. The states and input symbols ...This paper investigates the transition function and the reachability conditions of finite automata by using a semitensor product of matrices, which is a new powerful matrix analysis tool. The states and input symbols are first expressed in vector forms, then the transition function is described in an algebraic form. Using this algebraic representation, a sufficient and necessary condition of the reachability of any two states is proposed, based on which an algorithm is developed for discovering all the paths from one state to another. Furthermore, a mechanism is established to recognize the language acceptable by a finite automaton. Finally, illustrative examples show that the results/algorithms presented in this paper are suitable for both deterministic finite automata (DFA) and nondeterministic finite automata (NFA).展开更多
Objective In plant, squalene epoxidase (SE) catalyzes the first oxygenation step in the biosynthetic pathway of triterpenoid and phytosterol, representing one of the rate-limiting enzymes in this pathway. Bupleurum ...Objective In plant, squalene epoxidase (SE) catalyzes the first oxygenation step in the biosynthetic pathway of triterpenoid and phytosterol, representing one of the rate-limiting enzymes in this pathway. Bupleurum chinense is an important medicinal herb with its major active constituents such as triterpenoid saponins and saikosaponins. In order to obtain the series of enzymatic genes involved in saikosaponin biosynthesis, a cDNA of SE, designated BcSEI, was cloned from B. chinense. Methods The BcSEI gene was cloned by homology-based PCR and 5'/3' RACE methods from the adventitious roots of B. chinense. The physical and chemical parameters of BcSE1 protein were predicted by protparam. In order to discover hints in amino acid sequences on the dominant functions in the biosynthesis of saponin or phytosterol, sequences of SE from other plants were downloaded from NCBI for sequences alignment and phylogenetic analysis. BcSEI was cloned into a yeast mutant KLNI (MATa, ergl.':URA3, leu2, ura3, and trpl) to verify the enzyme activity of BcSE1. Additionally, the tissue-specific expression and methyl jasmonate (MeJA) inducibility of BcSEI were investigated using quantitative real-time PCR. Results The predicted protein of BcSE1 is highly similar to SEs from other plants sharing amino acid sequence identities of up to 88%. The BcSEI can functionally complement with yeast SE gene (ERGI) when expressed in the KLNI mutant (MATa, ergl::URA3, leu2, ura3, and trpl). Using as controls with ^-amyrin synthase (G-AS) which is presumed to catalyze the first committed step in saikosaponin biosynthesis and a cycloartenol synthase (CAS) relating to the phytosterol biosynthesis, the transcript of BcSE1 was significantly elevated by MeJA in adventitious roots of B. chinenseand the transcript of BcSElwas most abundant in the fruits and flowers of plants, followed by that in the leaves and roots, and least in stems. Conclusion It is the first time to illustrate the molecular information of SE in B. chinense and to clone the full-length SEgene in plants of genus Bupleurum L.展开更多
In the past,rice hybrids with strong heterosis have been obtained empirically,by developing and testing thousands of combinations.Here,we aimed to determine whether heterosis of an elite hybrid could be achieved by ma...In the past,rice hybrids with strong heterosis have been obtained empirically,by developing and testing thousands of combinations.Here,we aimed to determine whether heterosis of an elite hybrid could be achieved by manipulating major quantitative trait loci.We used 202 chromosome segment substitution lines from the elite hybrid Shanyou 63 to evaluate single segment heterosis(SSH)of yield per plant and identify heterotic loci.All nine detected heteroticloci acted in a dominant fashion,and no SSH exhibited overdominance.Functional alleles of key yield-related genes Ghd7,Ghd7.1,Hd1,and GS3 were dispersed in both parents.No functional alleles of three investigated genes were expressed at higher levels in the hybrids than in the more desirable parents.A hybrid pyramiding eight heterotic loci in the female parent Zhenshan 97 background had a comparable yield to Shanyou 63 and much higher yield than Zhenshan 97.Five hybrids pyramiding eight or nine heterotic loci in the combined parental genome background showed similar yield performance to that of Shanyou 63.These results suggest that dominance underlying functional complementation is an important contributor to yield heterosis and that heterosis assembly might be successfully promised by manipulating several major dominant heterotic loci.展开更多
The Hankel transform is widely used to solve various engineering and physics problems,such as the representation of electromagnetic field components in the medium,the representation of dynamic stress intensity factors...The Hankel transform is widely used to solve various engineering and physics problems,such as the representation of electromagnetic field components in the medium,the representation of dynamic stress intensity factors,vibration of axisymmetric infinite membrane and displacement intensity factors which all involve this type of integration.However,traditional numerical integration algorithms cannot be used due to the high oscillation characteristics of the Bessel function,so it is particularly important to propose a high precision and efficient numerical algorithm for calculating the integral of high oscillation.In this paper,the improved Gaver-Stehfest(G-S)inverse Laplace transform method for arbitrary real-order Bessel function integration is presented by using the asymptotic characteristics of the Bessel function and the accumulation of integration,and the optimized G-S coefficients are given.The effectiveness of the algorithm is verified by numerical examples.Compared with the linear transformation accelerated convergence algorithm,it shows that the G-S inverse Laplace transform method is suitable for arbitrary real order Hankel transform,and the time consumption is relatively stable and short,which provides a reliable calculation method for the study of electromagnetic mechanics,wave propagation,and fracture dynamics.展开更多
文摘Based on the Prototype Theory,the prototypical feature of advertisement is found to be the combination of three language functions:the informative function,the expressive function,and the vocative function.The advertisement translation means the adjustment of the informative function and the expressive function according to the differences between languages or cultures in order to maximize the vocative function.The faithful translation is the closest to the prototype of the source text but not necessarily the best translation.
文摘The expression and function in growth and apoptosis of the renin-angiotensin system(RAS)was evaluated inhuman glioblastoma.Renin and angiotensinogen(AGT)mRNAs and proteins were found by in situ hybridisationand immunohistochemistry in glioblastoma cells.Angiotensinogen was present in glioblastoma cystic fluids.Thus,human glioblastoma cells produce renin and AGT and secrete AGT.Human glioblastoma and glioblastoma cellsexpressed renin,AGT,renin receptor,AT(2)and/or AT(1)mRNAs and proteins determined by RT-PCR and/
文摘In this paper, it is pointed that the general expression for the stress function of the plane problem in polar coordinates is incomplete. The problems of the curved bar with an arbitrary distributive load at the boundries can't he solved by this stress function. For this reason, we suggest two new stress functions and put them into the general expression. Then, the problems of the curved bar applied with an arbitrary distributive load at r=a,b boundaries can be solved. This is a new stress function including geometric boundary constants.
文摘Fiber cell initiation is a complex process involving many pathways,including phytohormones and components for transcriptional and posttranscriptional regulation.Here we report expression
基金Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant No. 61174094), and the Tianjin Natural Science Foundation of China under (14JCYBJC18700 and 13JCY- BJC17400).
文摘This paper investigates the transition function and the reachability conditions of finite automata by using a semitensor product of matrices, which is a new powerful matrix analysis tool. The states and input symbols are first expressed in vector forms, then the transition function is described in an algebraic form. Using this algebraic representation, a sufficient and necessary condition of the reachability of any two states is proposed, based on which an algorithm is developed for discovering all the paths from one state to another. Furthermore, a mechanism is established to recognize the language acceptable by a finite automaton. Finally, illustrative examples show that the results/algorithms presented in this paper are suitable for both deterministic finite automata (DFA) and nondeterministic finite automata (NFA).
基金Open Research Fund of State Key Laboratory Breeding Base of Systematic Research,Development and Utilization of Chinese Medicine Resources 2014KFJJ05
文摘Objective In plant, squalene epoxidase (SE) catalyzes the first oxygenation step in the biosynthetic pathway of triterpenoid and phytosterol, representing one of the rate-limiting enzymes in this pathway. Bupleurum chinense is an important medicinal herb with its major active constituents such as triterpenoid saponins and saikosaponins. In order to obtain the series of enzymatic genes involved in saikosaponin biosynthesis, a cDNA of SE, designated BcSEI, was cloned from B. chinense. Methods The BcSEI gene was cloned by homology-based PCR and 5'/3' RACE methods from the adventitious roots of B. chinense. The physical and chemical parameters of BcSE1 protein were predicted by protparam. In order to discover hints in amino acid sequences on the dominant functions in the biosynthesis of saponin or phytosterol, sequences of SE from other plants were downloaded from NCBI for sequences alignment and phylogenetic analysis. BcSEI was cloned into a yeast mutant KLNI (MATa, ergl.':URA3, leu2, ura3, and trpl) to verify the enzyme activity of BcSE1. Additionally, the tissue-specific expression and methyl jasmonate (MeJA) inducibility of BcSEI were investigated using quantitative real-time PCR. Results The predicted protein of BcSE1 is highly similar to SEs from other plants sharing amino acid sequence identities of up to 88%. The BcSEI can functionally complement with yeast SE gene (ERGI) when expressed in the KLNI mutant (MATa, ergl::URA3, leu2, ura3, and trpl). Using as controls with ^-amyrin synthase (G-AS) which is presumed to catalyze the first committed step in saikosaponin biosynthesis and a cycloartenol synthase (CAS) relating to the phytosterol biosynthesis, the transcript of BcSE1 was significantly elevated by MeJA in adventitious roots of B. chinenseand the transcript of BcSElwas most abundant in the fruits and flowers of plants, followed by that in the leaves and roots, and least in stems. Conclusion It is the first time to illustrate the molecular information of SE in B. chinense and to clone the full-length SEgene in plants of genus Bupleurum L.
基金supported by grants from the National Key Research and Development Program of China(2016YFD0100802)the National Natural Science Foundation of China(31821005 and 31771751)。
文摘In the past,rice hybrids with strong heterosis have been obtained empirically,by developing and testing thousands of combinations.Here,we aimed to determine whether heterosis of an elite hybrid could be achieved by manipulating major quantitative trait loci.We used 202 chromosome segment substitution lines from the elite hybrid Shanyou 63 to evaluate single segment heterosis(SSH)of yield per plant and identify heterotic loci.All nine detected heteroticloci acted in a dominant fashion,and no SSH exhibited overdominance.Functional alleles of key yield-related genes Ghd7,Ghd7.1,Hd1,and GS3 were dispersed in both parents.No functional alleles of three investigated genes were expressed at higher levels in the hybrids than in the more desirable parents.A hybrid pyramiding eight heterotic loci in the female parent Zhenshan 97 background had a comparable yield to Shanyou 63 and much higher yield than Zhenshan 97.Five hybrids pyramiding eight or nine heterotic loci in the combined parental genome background showed similar yield performance to that of Shanyou 63.These results suggest that dominance underlying functional complementation is an important contributor to yield heterosis and that heterosis assembly might be successfully promised by manipulating several major dominant heterotic loci.
基金Supported by the National Natural Science Foundation of China(42064004,12062022,11762017,11762016)
文摘The Hankel transform is widely used to solve various engineering and physics problems,such as the representation of electromagnetic field components in the medium,the representation of dynamic stress intensity factors,vibration of axisymmetric infinite membrane and displacement intensity factors which all involve this type of integration.However,traditional numerical integration algorithms cannot be used due to the high oscillation characteristics of the Bessel function,so it is particularly important to propose a high precision and efficient numerical algorithm for calculating the integral of high oscillation.In this paper,the improved Gaver-Stehfest(G-S)inverse Laplace transform method for arbitrary real-order Bessel function integration is presented by using the asymptotic characteristics of the Bessel function and the accumulation of integration,and the optimized G-S coefficients are given.The effectiveness of the algorithm is verified by numerical examples.Compared with the linear transformation accelerated convergence algorithm,it shows that the G-S inverse Laplace transform method is suitable for arbitrary real order Hankel transform,and the time consumption is relatively stable and short,which provides a reliable calculation method for the study of electromagnetic mechanics,wave propagation,and fracture dynamics.