Yuan Longping proposed that smash-ridging technology could be extend-ed widely nationwide. ln the research, smash-ridging technology reconstruct cultiva-tion layers with loose soils in agricultural fields, creating ＂...Yuan Longping proposed that smash-ridging technology could be extend-ed widely nationwide. ln the research, smash-ridging technology reconstruct cultiva-tion layers with loose soils in agricultural fields, creating ＂4453＂ effects, as fol ows： ＂Four increases＂ include to increase loosen soil quantity in cultivation layers, soil nutri-ents use, ＂water pool＂ in soils, and ＂oxygen pool＂ in soils. Four reductions are to reduce soil erosion, carbon emission, salt content and heavy metal in soils. Five resistances refer to improve crop resistance capacity in terms of drought, high tem-perature, lower temperature, disease and lodging. Three improvements indicate to enhance photosynthetic efficiency over 10%, yield in 10%-30% and quality over 5%. lt is researched that without additional chemical fertilizer, yield could increase by 10% by labor force, animal, tractor or smash-ridging machine. What’s more, by smash-ridging cultivation, the depth can be 20 times or higher compared with tractor cultivation, with looser soils. lt is estimated that if smash-ridging cultivation is applied once in agricultural lands in China, present soil layers can be twice as deep as present. Specifical y, the thickness of loose soil-layers could be extended from 10-18 cm at present to 25-35 cm, and natural rainfal would increase by 40 bil ion cm3. After vitalization of soil nutrients, chemical fertilizer would decrease by 7 bil ion kg, and the increased c rops would feed more than 300 mil ion population as per yield at 7 50 kg/hm2.展开更多
通过重叠延伸PCR(gene splicing by overlap extension,SOE-PCR)扩增黄曲霉寡聚-1,6-葡萄糖苷酶基因和酿酒酵母α-信号肽序列,定向重组到整合型表达载体pδRCMB中,并在CICC1346中实现分泌表达;然后通过同源建模、利用分子模拟软件Discov...通过重叠延伸PCR(gene splicing by overlap extension,SOE-PCR)扩增黄曲霉寡聚-1,6-葡萄糖苷酶基因和酿酒酵母α-信号肽序列,定向重组到整合型表达载体pδRCMB中,并在CICC1346中实现分泌表达;然后通过同源建模、利用分子模拟软件Discovery Studio 4.1分析其蛋白结构,以对硝基苯-α-D-葡萄糖吡喃苷(pNPG)为底物,建立并确定酶活反应条件,并进行纯化、酶学性质分析;将密码子优化后序列在CICC1346中实现分泌表达,利用淀粉进行共发酵实验。结果显示:重组酶突变后相对野生型蛋白结构无影响。SDS-PAGE分析重组重组酶大小约为70 kDa;优化后最高酶活达到0.69 U/m L,重组酶最适pH为6.5,在pH4.5.0~7.0维持90%以上的酶活;最适温度为40℃,在30~40℃维持接近100%的酶活;受Cu^(2+)和Mn^(2+)严格抑制;寡聚-1,6-葡萄糖苷酶与α-淀粉酶及糖化酶协同利用淀粉产乙醇,提高淀粉水解效率。这是首次报道黄曲霉的寡聚-1,6-葡萄糖苷酶基因在酿酒酵母整合型分泌表达。展开更多
We have calculated the orbital parameters for 90 stars in Chen et al. and updated the kinematic data for stars in Edvardsson et al. by using the accurate Hipparcos parallaxes and proper motions, and recalculated the ...We have calculated the orbital parameters for 90 stars in Chen et al. and updated the kinematic data for stars in Edvardsson et al. by using the accurate Hipparcos parallaxes and proper motions, and recalculated the α-element abundances in Edvardsson et al. in a way consistent with Chen et al. The two sets of data are combined in a study of stellar populations and characteristics of F & G stars in the solar neighborhood. We confirm the result of Chen et al. that a distinguishable group of stars may belong to the thick disk rather than the thin disk. The ages for the stars are determined using the theoretical isochrones of VandenBerg et al. The age-metallicity relation is investigated for different subgroups according to distance from the sun and galactic orbital parameters. It is found that a mixing of stars with different orbital parameters significantly affect the age-metallicity relation for the disk. Stars with orbits confined to the solar circle all have metallicities [Fe/H] > -0.3 irrespective of their distances from the sun or from the Galactic plane.展开更多
Based on the Hipparcos proper motions and available radial velocity data of O-B stars, we have re-examined the local kinematical structure of the young disk population of 1500 O-B stars not including the Gould-belt s...Based on the Hipparcos proper motions and available radial velocity data of O-B stars, we have re-examined the local kinematical structure of the young disk population of 1500 O-B stars not including the Gould-belt stars. A systematic warping motion of the stars about the direction to the Galactic center has been reconfirmed. A negative K-term implying a systematic contraction of stars in the solar vicinity has been detected. Two different distance scales are used in order to find out their impact on the kinematical parameters, and we conclude that the adopted distance scale plays an important role in characterizing the kinematical parameters at the present level of the measurement uncertainty.展开更多
针对传统密度峰值聚类算法(Clustering by fast search and find of density peaks,CFSFDP)簇心选择不佳、非簇心点分配不准确导致的聚类效果差问题,提出一种融合可拓关联函数的密度峰值聚类算法,通过引入平均差异度衡量样本密度选取簇...针对传统密度峰值聚类算法(Clustering by fast search and find of density peaks,CFSFDP)簇心选择不佳、非簇心点分配不准确导致的聚类效果差问题,提出一种融合可拓关联函数的密度峰值聚类算法,通过引入平均差异度衡量样本密度选取簇心;基于k邻域思想提出雏形簇概念并建立物元模式下的节域和经典域;利用可拓关联函数定量衡量未分配点相对各雏形簇的隶属程度,从而实现精准聚类.将该算法和其他几种聚类算法在人工数据集和UCI标准数据集上分别进行对比实验,实验结果表明本文算法对任意形状任意密度数据集的聚类效果更好,优于其他算法.展开更多
基金Supported by the Key Project of Science and Technology of Guangxi(1222014-2C)Achievements Transformation Project of Guangxi Academy of Agricultural Science and Technology(201405)Fundamental Research Funds for Guangxi Academy of Agricultural Science and Technology(2014YZ07)~~
文摘Yuan Longping proposed that smash-ridging technology could be extend-ed widely nationwide. ln the research, smash-ridging technology reconstruct cultiva-tion layers with loose soils in agricultural fields, creating ＂4453＂ effects, as fol ows： ＂Four increases＂ include to increase loosen soil quantity in cultivation layers, soil nutri-ents use, ＂water pool＂ in soils, and ＂oxygen pool＂ in soils. Four reductions are to reduce soil erosion, carbon emission, salt content and heavy metal in soils. Five resistances refer to improve crop resistance capacity in terms of drought, high tem-perature, lower temperature, disease and lodging. Three improvements indicate to enhance photosynthetic efficiency over 10%, yield in 10%-30% and quality over 5%. lt is researched that without additional chemical fertilizer, yield could increase by 10% by labor force, animal, tractor or smash-ridging machine. What’s more, by smash-ridging cultivation, the depth can be 20 times or higher compared with tractor cultivation, with looser soils. lt is estimated that if smash-ridging cultivation is applied once in agricultural lands in China, present soil layers can be twice as deep as present. Specifical y, the thickness of loose soil-layers could be extended from 10-18 cm at present to 25-35 cm, and natural rainfal would increase by 40 bil ion cm3. After vitalization of soil nutrients, chemical fertilizer would decrease by 7 bil ion kg, and the increased c rops would feed more than 300 mil ion population as per yield at 7 50 kg/hm2.
文摘通过重叠延伸PCR(gene splicing by overlap extension,SOE-PCR)扩增黄曲霉寡聚-1,6-葡萄糖苷酶基因和酿酒酵母α-信号肽序列,定向重组到整合型表达载体pδRCMB中,并在CICC1346中实现分泌表达;然后通过同源建模、利用分子模拟软件Discovery Studio 4.1分析其蛋白结构,以对硝基苯-α-D-葡萄糖吡喃苷(pNPG)为底物,建立并确定酶活反应条件,并进行纯化、酶学性质分析;将密码子优化后序列在CICC1346中实现分泌表达,利用淀粉进行共发酵实验。结果显示:重组酶突变后相对野生型蛋白结构无影响。SDS-PAGE分析重组重组酶大小约为70 kDa;优化后最高酶活达到0.69 U/m L,重组酶最适pH为6.5,在pH4.5.0~7.0维持90%以上的酶活;最适温度为40℃,在30~40℃维持接近100%的酶活;受Cu^(2+)和Mn^(2+)严格抑制;寡聚-1,6-葡萄糖苷酶与α-淀粉酶及糖化酶协同利用淀粉产乙醇,提高淀粉水解效率。这是首次报道黄曲霉的寡聚-1,6-葡萄糖苷酶基因在酿酒酵母整合型分泌表达。
基金NKBRSF G1999075406 the National Natural Science Foundation of China under grant 10173014.
文摘We have calculated the orbital parameters for 90 stars in Chen et al. and updated the kinematic data for stars in Edvardsson et al. by using the accurate Hipparcos parallaxes and proper motions, and recalculated the α-element abundances in Edvardsson et al. in a way consistent with Chen et al. The two sets of data are combined in a study of stellar populations and characteristics of F & G stars in the solar neighborhood. We confirm the result of Chen et al. that a distinguishable group of stars may belong to the thick disk rather than the thin disk. The ages for the stars are determined using the theoretical isochrones of VandenBerg et al. The age-metallicity relation is investigated for different subgroups according to distance from the sun and galactic orbital parameters. It is found that a mixing of stars with different orbital parameters significantly affect the age-metallicity relation for the disk. Stars with orbits confined to the solar circle all have metallicities [Fe/H] > -0.3 irrespective of their distances from the sun or from the Galactic plane.
基金Supported by the National Natural Science Foundation of China.
文摘Based on the Hipparcos proper motions and available radial velocity data of O-B stars, we have re-examined the local kinematical structure of the young disk population of 1500 O-B stars not including the Gould-belt stars. A systematic warping motion of the stars about the direction to the Galactic center has been reconfirmed. A negative K-term implying a systematic contraction of stars in the solar vicinity has been detected. Two different distance scales are used in order to find out their impact on the kinematical parameters, and we conclude that the adopted distance scale plays an important role in characterizing the kinematical parameters at the present level of the measurement uncertainty.
文摘针对传统密度峰值聚类算法(Clustering by fast search and find of density peaks,CFSFDP)簇心选择不佳、非簇心点分配不准确导致的聚类效果差问题,提出一种融合可拓关联函数的密度峰值聚类算法,通过引入平均差异度衡量样本密度选取簇心;基于k邻域思想提出雏形簇概念并建立物元模式下的节域和经典域;利用可拓关联函数定量衡量未分配点相对各雏形簇的隶属程度,从而实现精准聚类.将该算法和其他几种聚类算法在人工数据集和UCI标准数据集上分别进行对比实验,实验结果表明本文算法对任意形状任意密度数据集的聚类效果更好,优于其他算法.
