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Regulation of the growth of sprouting roots of black locust seedlings using root barrier panels 被引量:1
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作者 Satoshi Kitaoka Shiro Matsunami +4 位作者 Yannan Wang Saki Fujita Kobayashi Makoto Toshiyuki Hirata Takayoshi Koike 《Journal of Forestry Research》 SCIE CAS CSCD 2023年第3期655-664,共10页
How can we regulate an invasive alien species of high commercial value?Black locust(Robinia pseudoacacia L.)has a unique capacity for seed dispersal and high germination.Field surveys indicate that black locust increa... How can we regulate an invasive alien species of high commercial value?Black locust(Robinia pseudoacacia L.)has a unique capacity for seed dispersal and high germination.Field surveys indicate that black locust increases its growing area with sprouting roots and the elongation of horizontal roots at a soil depth of 10 cm.Therefore,a method to regulate the development of horizontal roots could be eff ective in slowing the invasiveness of black locust.In this study,root barrier panels were tested to inhibit the growth of horizontal roots.Since it is labor intensive to observe the growth of roots in the fi eld,it was investigated in a nursery setting.The decrease in secondary fl ush,an increase in yellowed leafl ets,and the height in the seedlings were measured.Installing root barrier panels to a depth of 30 cm eff ectively inhibit the growth of horizontal roots of young black locust. 展开更多
关键词 Black locust Horizontal roots NUTRIENTS Root barrier panel sprouting roots
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Identification of KASP markers and putative genes for pre-harvest sprouting resistance in common wheat(Triticum aestivum L.) 被引量:1
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作者 Guannan Liu Daniel Mullan +3 位作者 Aimin Zhang Hui Liu Dongcheng Liu Guijun Yan 《The Crop Journal》 SCIE CSCD 2023年第2期549-557,共9页
Common wheat(Triticum aestivum L.)is the most important crop in the world and a typical allopolyploid with a large and complex genome.Pre-harvest sprouting(PHS)leads to a significant reduction in grain quality worldwi... Common wheat(Triticum aestivum L.)is the most important crop in the world and a typical allopolyploid with a large and complex genome.Pre-harvest sprouting(PHS)leads to a significant reduction in grain quality worldwide.PHS is a complex trait with related QTL located on different chromosomes.However,the study of markers and genes related to PHS resistance is limited especially for whitegrained wheat.Four pairs of near isogenic lines(NILs)from a white-grained wheat cross of CharaDM5637B*8 targeting a major QTL for PHS resistance(Qphs.ccsu-3A.1)on wheat chromosme 3AL were genotyped using the 90K SNP Illumina iSelect array.Ten SNPs were identified,with a 75%-100%consistency between genotype and phenotype in the resistant or susceptible isolines.The 10 SNPs were converted to cost-effective kompetitive allele-specific PCR(KASP)markers.Screening of 48 wheat cultivars with different phenotypes of PHS identified four KASP markers with 81.3%-85.4%conformity between genotype and phenotype.Further investigation revealed that the four SNPs(BS00022245_51,Kukri_c49927_151,BS00022884_51 and BS00110550_51)corresponding to the four validated KASP markers are residing in three independent genes(TraesCS3A03G1072800,TraesCS3A03G1072400,TraesCS3A03G1071800)close to each other with a distance of 4.28-4.48 Mb to the targeted QTL.These three annotated genes have potential functions related to PHS resistance.Our study revealed that combined use of NILs and the 90K SNP chip is a powerful approach for developing KASP markers and mining functional genes in wheat.The KASP markers for PHS resistance on chromosome 3AL are useful for high-throughput evaluation and marker-assisted selection,and the three identified genes could lead to a better understanding of the genetic pathways controlling PHS. 展开更多
关键词 Pre-harvest sprouting(PHS) KASP marker Functional genes Chromosome 3AL 90K SNP assay Near-isogenic lines
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QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22
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作者 Rabiu Sani Shawai Dan Liu +7 位作者 Lingli Li Tiantian Chen Ming Li Shuanghe Cao Xianchun Xia Jindong Liu Zhonghu He Yong Zhang 《The Crop Journal》 SCIE CSCD 2023年第3期863-869,共7页
Pre-harvest sprouting(PHS)is one of the serious global issues in wheat production.Identification of quantitative trait loci(QTL)and closely-linked markers is greatly helpful for wheat improvement.In the present study,... Pre-harvest sprouting(PHS)is one of the serious global issues in wheat production.Identification of quantitative trait loci(QTL)and closely-linked markers is greatly helpful for wheat improvement.In the present study,a recombinant inbred line(RIL)population derived from the cross of Zhongmai 578(ZM578)/Jimai 22(JM22)and parents were phenotyped in five environments and genotyped by the wheat 50 K single-nucleotide polymorphism(SNP)array.Two QTL of germination index(GI),QGI.caas-3A and QGI.caas-5A,were detected,explaining 4.33%–5.58%and 4.43%–8.02%of the phenotypic variances,respectively.The resistant effect of QGI.caas-3A was contributed by JM22,whereas that of QGI.caas.5A was from ZM578.The two QTL did not correspond to any previously identified genes or genetic loci for PHSrelated traits according to their locations in the Chinese Spring reference genome,indicating that they are likely to be new loci for PHS resistance.Four kompetitive allele-specific PCR(KASP)markers K_AX-109605367and K_AX-179559687 flanking QGI.caas-3A,and K_AX-111258240 and K_AX-109402944flanking QGI.caas-5A,were developed and validated in a natural population of 100 wheat cultivars.The distribution frequency of resistance alleles at Qphs.caas-3A and Qphs.caas-5A loci were 82.7%and57.1%,respectively,in the natural population.These findings provide new QTL and tightly linked KASP markers for improvement of PHS resistance in wheat. 展开更多
关键词 Pre-harvest sprouting Germination index Quantitative trait loci KASP marker Triticum aestivum L.
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Evaluation of Pre-Harvest Sprouting(PHS)Resistance and Screening of High-Quality Varieties from Thirty-Seven Quinoa(Chenopodium quinoa Willd.)Resources in Chengdu Plain
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作者 Xin Pan Ya Gao +5 位作者 Fang Zeng Chunmei Zheng Wenxuan Ge Yan Wan Yanxia Sun Xiaoyong Wu 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第10期2921-2936,共16页
Pre-harvest sprouting(PHS)will have a serious effect both on the yield and quality of quinoa(Chenopodium quinoaWilld.).It is crucial to select and breed quinoa varieties with PHS resistance and excellent agronomic tra... Pre-harvest sprouting(PHS)will have a serious effect both on the yield and quality of quinoa(Chenopodium quinoaWilld.).It is crucial to select and breed quinoa varieties with PHS resistance and excellent agronomic traits for guidance production and utilization of quinoa.A comprehensive evaluation of the PHS resistance and agronomic traits of 37 species of quinoa resources was conducted in Chengdu Plain.The evaluation used various methods,including grain germination rate(GR),grain germination index(GI),total spike germination rate(SR),total grain germination index(SI),grey correlation analysis(GCA),cluster analysis and correlation analysis.Results showed significant differences in PHS resistance and agronomic traits amongst the 37 quinoa resources.CDU-23 was most resistant to PHS within 24 h,with a germination rate of 2.67%and 0%according to the GR and SR results,respectively.However,in the same time,CDU-31 showed the maximum susceptibility to PHS based on the SR of 31.07%,while CDU-34 was the most sensitive to PHS according to the GR of 100%.The comprehensive evaluation identified one and nine kinds of high resistance species for grain and whole spike germination,respectively.In both cases,the coefficients of variation(CV)for these parameters were 34.78%and 82.13%,respectively.GCA results showed that the magnitude of the association between each trait and yield in the thirty-seven quinoa resources was in the following order:thousand grain weight>seed length>seed area>seed width.Although the seed weight of CDU-18 reached 3.7010 g,the seed weight of CDU-5 was only 1.6030 g.However,the size of the seeds,their width and area did not correlate with their 1000-grain weight.There was a complex correlation between PHS resistance index and agronomic traits.Based on clustering analysis,thirty-seven quinoa resources were classified into three taxa.It was found that various taxa differed in PHS resistance and agronomic traits.Several comparisons of the aggregated data led to the selection of five varieties of quinoa,of which CDU-2 presented excellent agronomic qualities and strong PHS resistance.This study has provided a reference for breeding excellent quinoa varieties with PHS resistance. 展开更多
关键词 QUINOA pre-harvest sprouting(PHS) agronomic traits cluster analysis
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UV-B irradiation enhances the accumulation of beneficial glucosinolates induced by melatonin in Chinese kale sprout
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作者 Mengyu Wang Jiansheng Wang +6 位作者 Yuhe Yang Zhiqing Li Ghazala Mustafa Yubo Li Lihong Liu Zuofa Zhang Qiaomei Wang 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第4期995-1006,共12页
Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by appl... Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by applying different chemical regulators,with a particular focus on their contribution to nutritional quality and health benefits.Nevertheless,the effects of melatonin and UV-B irradiation on glucosinolate biosynthesis remain unclear.In this study,it was found that changes in melatonin concentrations significantly affected the contents of individual as well as total aliphatic and indolic glucosinolates.The 5μmol·L^(-1)melatonin was decided as the optimum concentration that could increase the content of beneficial glucosinolates including glucoraphanin and 4-methoxy glucobrassicin in Chinese kale sprouts.Notably,the enhancement of glucosinolate accumulation by melatonin treatment could be further amplified by UV-B irradiation.Furthermore,our results showed that R2R3-MYB transcription factor BoaMYB28 and BoaMYB51,which are central regulators of aliphatic and indolic glucosinolate biosynthesis respectively,were both involved in the regulation of glucosinolate biosynthesis by melatonin and UV-B irradiation.Additionally,the expression of glucosinolate biosynthetic genes,including BoaCYP79F1,BoaCYP83A1,BoaSUR1,BoaUGT74B1,BoaCYP79B2,BoaCYP79B3,and BoaCYP83B1 participated in the formation of core structures and BoaFMOGS-OX5,BoaAOP2,BoaCYP81F2,and BoaIGMT1 involved in the sidechain modification of aliphatic and indolic glucosinolate,was regulated by melatonin or UV-B irradiation.Taken together,these findings provide a potential strategy for improving the nutritional quality and resistance of Chinese kale sprouts. 展开更多
关键词 MELATONIN Chinese kale sproutS UV-B GLUCOSINOLATES Nutritional quality Second metabolism
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Comparative study of the effects of Tartary buckwheat seed and sprout consumption on the physiological indices and gut microbiota of C57BL/6J mice
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作者 Guohui Nan Haixia Zhao +6 位作者 Qiong Wu Lisong Liu Zichao Guan Chenglei Li Huala Wu Dabing Xiang Qi Wu 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期791-800,共10页
Tartary buckwheat(Fagopyrum tataricum)is a well-known pseudocereal for its health and economic value.However,abundant antinutritional factors(ANFs)reduces its health benefits.As reported,germination can improve the nu... Tartary buckwheat(Fagopyrum tataricum)is a well-known pseudocereal for its health and economic value.However,abundant antinutritional factors(ANFs)reduces its health benefits.As reported,germination can improve the nutritional profile of grains.In this study,we systematically evaluate the safety of Tartary buckwheat seeds(TB)and Tartary buckwheat sprouts(TBS)used as high active ingredients.After evaluating nutrition levels,bioactive compounds and ANFs in TBS during germinating,5^(th)-day TBS were selected as the raw material.C57BL/6J mice were gavaged daily with distilled water,TB,or TBS for 6 weeks.The physiological indices related to ANFs were determined.Results showed that the TB intake tends to generate negative effects on the gut microbiota,and organs.Additionally,upon TB intake,the Fe^(3+)content in serum,trypsin activity in pancreas and jejunum decreased,while the cytokine,IgE,and histamine levels in serum,water content in faeces,cytokine levels in liver and jejunum increased.Conversely,TBS did not induce any obvious negative effects on the above relevant indices and showed better lipid-lowering effect.Altogether,TBS are safer and more effective as a raw material to produce the functional food for long-term consumption with the intention of preventing and treating hyperlipidaemia. 展开更多
关键词 Tartary buckwheat Tartary buckwheat sprouts Antinutritional factors Gut microbiota Health effect
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Effect of Light Emitting Diodes (LEDs) on Growth, Mineral Composition, and Nutritional Value of Wheat & Lentil Sprouts
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作者 Abdul Momin Amana Khatoon +10 位作者 Wajahat Khan Dilsat Bozdoğan Konuşkan Muhammad Mudasar Aslam Muhammad Jamil Shafiq Ur Rehman Baber Ali Alevcan Kaplan Sana Wahab Muhammad Nauman Khan Sezai Ercisli Mohammad Khalid Al-Sadoon 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第6期1117-1128,共12页
Sprouts are ready-to-eat and are recognized worldwide as functional components of the human diet.Recent advances in innovative agricultural techniques could enable an increase in the production of healthy food.The use... Sprouts are ready-to-eat and are recognized worldwide as functional components of the human diet.Recent advances in innovative agricultural techniques could enable an increase in the production of healthy food.The use of light-emitting diode(LED)in indoor agricultural production could alter the biological feedback loop,increasing the functional benefits of plant foods such as wheat and lentil sprouts and promoting the bioavailability of nutrients.The effects of white(W),red(R),and blue(B)light were investigated on the growth parameters and nutritional value of wheat and lentil sprouts.In the laboratory,seeds were sown under three different LED treat-ments:white,red,and blue light,while normal incandescent light served as a control.Percentage seed germina-tion improved by 18.34%and 12.67%for wheat and 18.34%and 12.67%for lentil sprouts under LED treatments R and B,respectively.An increase in total soluble protein and sugar by 33.4%and 9.23%in wheat and by 31.5%and 5.87%in lentils was observed under the R LED treatment.Vitamin C concentrations in wheat and lentils were significantly increased by R LED compared to all other treatments.Other parameters,including potassium and sodium concentrations,were significantly increased under red and blue light compared to the control;white light,on the other hand,significantly decreased all these parameters.According to the experimental data,red and blue LED light could be beneficial in the production of functional wheat and lentil sprouts with high nutrient concentrations. 展开更多
关键词 Growth parameters LED nutritional value sproutS total soluble proteins
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Transcriptomics integrated with metabolomics reveals the mechanism of CaCl_(2)-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts
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作者 Cui Li Shuhui Song +1 位作者 Yanan He Haijie Liu 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期801-812,共12页
Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment ... Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment significantly decreased reactive oxygen species(ROS)and malondialdeh yde(MDA)contents in broccoli sprouts.On the the 8^(th)day,compared to tap water treatment,the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6%and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW.Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades.Besides,CHEW treatment not only promoted the biosynthesis of amino acids,but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors(MYBs,bHLHs,WRKYs,etc.).The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment. 展开更多
关键词 Broccoli sprouts CaCl_(2)-HCl electrolyzed water GLUCOSINOLATES TRANSCRIPTOMICS Metabolomics
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Experimental Research on Four Season Soilless Cultivation of the Sprouting Seedlings of Toona sinensis in Chongqing 被引量:1
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作者 王正春 辛静 蒋海艳 《Agricultural Science & Technology》 CAS 2017年第9期1662-1665,共4页
Using seed sowing cultivation method, the four-season soilless cultivation of the sprouting seedlings of Toona sinensis was carried out with different germination accelerating treatments, cultivation substrates, culti... Using seed sowing cultivation method, the four-season soilless cultivation of the sprouting seedlings of Toona sinensis was carried out with different germination accelerating treatments, cultivation substrates, cultivation environment and management technologies by using the corresponding treatment methods in different seasons, so as to produce the sprouting vegetable products of T. sinensis which was not restricted by seasons. The test results showed that using the mixed substrate of pedite and vermiculite in winter and spring can keep moisture but not too high, and the produced sprout vegetable had good quality and high yield. 展开更多
关键词 CHONGQING sprouting seedlings of Toona sinensis Four-season soilless cultivation
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Effects of Fragmentation Intensity of Perennial Roots and Their Burial Depth on Sprouting and Early Growth of Alternanthera philoxeroides(Mart.)Griseb
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作者 陈中义 邹云胜 +2 位作者 陈燕丽 张中华 许兴蕾 《Agricultural Science & Technology》 CAS 2010年第6期103-105,111,共4页
[Objective] The aim was to provide theoretical basis for the prevention and control of the invasion of Alternanthera philoxeroides(Mart.)Griseb.[Method] Effects of fragmentation intensity of fresh roots and their bu... [Objective] The aim was to provide theoretical basis for the prevention and control of the invasion of Alternanthera philoxeroides(Mart.)Griseb.[Method] Effects of fragmentation intensity of fresh roots and their burial depth on sprouting and early growth of A.philoxeroides were studied by control test.[Result] More sprouts of A.philoxeroides emerged when the fragmentation intensity of fresh roots was higher,while if the fragmentation intensity of fresh roots was lower,the early growth of A.philoxeroides was more rapid.The soil buried depth had significant effect on fresh root sprouts' emergence,but once fresh root sprouts could reach the soil surface and were given enough growth time,even if the fresh roots were buried in different depths,soil buried depth had no significant effect on its young plant growth.[Conclusion] If different fragmentation intensities of fresh roots present,there is a kind of trade-off strategy between root sprouts' emergence and plant' early growth,by which A.philoxeroides can invade new habitat successfully.To control the invasion of A.philoxeroides,it is critical to prevent its fresh root sprouts from emerging to soil surface,that is,to bury the fresh roots at a further soil depth. 展开更多
关键词 Alternanthera philoxeroides(Mart.)Griseb Perennial root fragment Soil buried depth sprouting Young plant growth
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Tolerant Mechanism and Chromosome Location of Gene Controlling Sprouting Tolerance in Aegilops Tauschii Cosson 被引量:4
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作者 LAN Xiu-jin, ZHENG You-liang, LIU Deng-cai, WEI Yu-ming, YAN Zehong and ZHOU Yong-hong(Triticeae Research Institute , Sichuan Agricultural University, Dujiangyan 611830) 《Agricultural Sciences in China》 CAS CSCD 2002年第3期265-268,共4页
An artificial amphiploid RSP (2n = 42, AABBDD) between tetraploid landrace Ailanmai (Triticum turgidum L., 2n= 28, AABB) and Aegllops tauschii (DD, 2n = 14) expressed high tolerance to preharvest sprouting which deriv... An artificial amphiploid RSP (2n = 42, AABBDD) between tetraploid landrace Ailanmai (Triticum turgidum L., 2n= 28, AABB) and Aegllops tauschii (DD, 2n = 14) expressed high tolerance to preharvest sprouting which derived from Ae. tauschii. Tolerance to preharvest sprouting of RSP was examined by four ways in six varying periods after anthesis. The germination percentages of preharvest intact spikes were only 6.06% in its high peak period of germination. Its tolerance was mainly decided by the seed dormancy. It was showed that the tolerance to sprouting in ' RSP' derived from Ae. tauschii was inherited as a recessive trait which was controlled by one gene, located on chromosome 2D. 展开更多
关键词 Aegilops tauschii Breeding Wheat Tolerance to sprouting Gene Location
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QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6 被引量:1
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作者 ZHOU Sheng-hui FU Lin +12 位作者 WU Qiu-hong CHEN Jiao-jiao CHEN Yong-xing XIE Jing-zhong WANG Zhen-zhong WANG Guo-xin ZHANG De-yun LIANG Yong ZHANG Yan YOU Ming-shan LIANG Rong-qi HAN Jun LIU Zhi-yong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第2期435-444,共10页
Pre-harvest sprouting (PHS) occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting in substantial economic loss. In this study, quantitative tr... Pre-harvest sprouting (PHS) occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting in substantial economic loss. In this study, quantitative trait loci (QTL) for PHS resistance were mapped using an available high-density single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) genetic linkage map developed from a 269 recombinant inbred lines (RILs) population of Yanda 1817xBeinong 6. Using phenotypic data on two locations (Beijing and Shijiazhuang, China) in two years (2012 and 2013 harvesting seasons), five QTLs, designated as QPhs.cau-3A. 1, QPhs.cau-3A.2, QPhs.cau-5B, QPhs.cau-4A, and QPhs.cau-6A, for PHS (GP) were detected by inclusive composite interval mapping (ICIM) (LOD≥2.5). Two major QTLs, QPhs.cau-3A.2 and QPhs.cau-5B, were mapped on 3AL and 5BS chromosome arms, explaining 6.29-21.65% and 4.36-5.94% of the phenotypic variance, respectively. Precise mapping and comparative genomic analysis revealed that the TaVp-1A flanking region on 3AL is responsible for QPhs.cau-3A.2. SNP markers flanking QPhs.cau-3A.2 genomic region were developed and could be used for introgression of PHS tolerance into high yielding wheat varieties through marker-assisted selection (MAS). 展开更多
关键词 WHEAT pre-harvest sprouting quantitative trait loci SNP TaVp-IA
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Axonal regeneration and sprouting as a potential therapeutic target for nervous system disorders 被引量:3
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作者 Katherine L.Marshall Mohamed H.Farah 《Neural Regeneration Research》 SCIE CAS CSCD 2021年第10期1901-1910,共10页
Nervous system disorders are prevalent health issues that will only continue to increase in frequency as the population ages.Dying-back axonopathy is a hallmark of many neurologic diseases and leads to axonal disconne... Nervous system disorders are prevalent health issues that will only continue to increase in frequency as the population ages.Dying-back axonopathy is a hallmark of many neurologic diseases and leads to axonal disconnection from their targets,which in turn leads to functional impairment.During the course of many of neurologic diseases,axons can regenerate or sprout in an attempt to reconnect with the target and restore synapse function.In amyotrophic lateral sclerosis(ALS),distal motor axons retract from neuromuscular junctions early in the disease-course before significant motor neuron death.There is evidence of compensatory motor axon sprouting and reinnervation of neuromuscular junctions in ALS that is usually quickly overtaken by the disease course.Potential drugs that enhance compensatory sprouting and encourage reinnervation may slow symptom progression and retain muscle function for a longer period of time in ALS and in other diseases that exhibit dying-back axonopathy.There remain many outstanding questions as to the impact of distinct disease-causing mutations on axonal outgrowth and regeneration,especially in regards to motor neurons derived from patient induced pluripotent stem cells.Compartmentalized microfluidic chambers are powerful tools for studying the distal axons of human induced pluripotent stem cells-derived motor neurons,and have recently been used to demonstrate striking regeneration defects in human motor neurons harboring ALS disease-causing mutations.Modeling the human neuromuscular circuit with human induced pluripotent stem cells-derived motor neurons will be critical for developing drugs that enhance axonal regeneration,sprouting,and reinnervation of neuromuscular junctions.In this review we will discuss compensatory axonal sprouting as a potential therapeutic target for ALS,and the use of compartmentalized microfluidic devices to find drugs that enhance regeneration and axonal sprouting of motor axons. 展开更多
关键词 amyotrophic lateral sclerosis axonal regeneration dying-back axonopathy in vitro neuromuscular junction iPSC-derived motor neurons microfluidic device motor axon sprouting
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Changes in biochemical constituents and induction of early sprouting by triadimefon treatment in white yam(Dioscorea rotundata Poir.) tubers during storage 被引量:1
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作者 JALEEL Cheruth Abdul GOPI Ragupathi +3 位作者 MANIVANNAN Paramasivam KISHOREKUMAR Ashok GOMATHINAYAGAM Muthiah PANNEERSELVAM Rajaram 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第4期283-288,共6页
The ability of triadimefon (TDM), a triazolic fungicide, to alter the biochemical constituents and thereby minimizing the days required for sprouting in white yam (Dioscorea rotundata Poir.) tubers during storage ... The ability of triadimefon (TDM), a triazolic fungicide, to alter the biochemical constituents and thereby minimizing the days required for sprouting in white yam (Dioscorea rotundata Poir.) tubers during storage under (30±2) ℃ in the dark, was studied. TDM at 20 mg/L was given to tubers by dipping the tubers in treatment solution containing 20 mg/L TDM on 10, 25 and 40 d after storage (DAS). Starch, sugars, protein, amino acid contents as well as protease and a-amylase activities were estimated on 15, 30 and 45 DAS from two physiological regions viz., apical and basal regions of the tubers. In normal conditions (control) sprouting occurred on 70 to 80 DAS. The starch content decreased, while protein, amino acid, sugar contents and protease and a-amylase activities were increased due to TDM treatment and led to early sprouting. 展开更多
关键词 Biochemical constituents Enzyme activities Dioscorea rotundata sprouting STORAGE Triadimefon (TDM)
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Extrinsic inhibitors in axon sprouting and functional recovery after spinal cord injury 被引量:1
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作者 Jessica M.Meves Binhai Zheng 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第5期460-461,共2页
The limited axonal growth after central nervous system (CNS) injury such as spinal cord injury presents a major challenge in promoting repair and recovery. The literature in axonal repair has focused mostly on frank... The limited axonal growth after central nervous system (CNS) injury such as spinal cord injury presents a major challenge in promoting repair and recovery. The literature in axonal repair has focused mostly on frank regeneration of injured axons. Here, we argue that sprouting of uninjured axons, an innate repair mech- anism of the CNS, might be more amenable to modulation in order to promote functional repair. Extrinsic inhibitors of axonal growth modulate axon sprouting after injury and may serve as the first group of therapeutic targets to promote functional repair. 展开更多
关键词 CNS OMgp Extrinsic inhibitors in axon sprouting and functional recovery after spinal cord injury
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Recent evidence for activity-dependent initiation of sympathetic sprouting and neuropathic pain 被引量:10
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作者 Jun-Ming ZHANG Judith A. Strong 《生理学报》 CAS CSCD 北大核心 2008年第5期617-627,共11页
Traumatic injury or inflammatory irritation of the peripheral nervous system often leads to persistent pathophysiological pain states.It has been well-documented that,after peripheral nerve injury or inflammation,func... Traumatic injury or inflammatory irritation of the peripheral nervous system often leads to persistent pathophysiological pain states.It has been well-documented that,after peripheral nerve injury or inflammation,functional and anatomical alterations sweep over the entire peripheral nervous system including the peripheral nerve endings,the injured or inflamed afferent fibers,the dorsal root ganglion(DRG),and the central afferent terminals in the spinal cord.Among all the changes,ectopic discharge or spontaneous activity of primary sensory neurons is of great clinical interest,as such discharges doubtless contribute to the develop-ment of pathological pain states such as neuropathic pain.Two key sources of abnormal spontaneous activity have been identified following peripheral nerve injury:the injured afferent fibers(neuroma) leading to the DRG,and the DRG somata.The purpose of this review is to provide a global account of the abnormal spontaneous activity in various animal models of pain.Particular attention is focused on the consequence of peripheral nerve injury and localized inflammation.Further,mechanisms involved in the generation of spontaneous activity are also reviewed;evidence of spontaneous activity in contributing to abnormal sympathetic sprouting in the axotomized DRG and to the initiation of neuropathic pain based on new findings from our research group are discussed.An improved understanding of the causes of spontaneous activity and the origins of neuropathic pain should facilitate the development of novel strategies for effective treatment of pathological pain. 展开更多
关键词 活动依赖性 神经性疼痛 病症 临床分析
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The association between the allelic state of <i>Vp</i>-1<i>B</i>and pre-harvest sprouting tolerance in red-seeded hexaploid triticale
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作者 Mikhail Divashuk Nikolay Mayer +5 位作者 Pavel Kroupin Valentina Rubets Vladimir Pylnev Nguen Tkhi Tkhu Lin Alexandr Soloviev Gennady Karlov 《Open Journal of Genetics》 2012年第1期51-55,共5页
The core collection of red-seeded winter hexaploid triticale with different pre-harvest sprouting (PHS) resistance has been evaluated for the allelic structure of the gene VIVIPAROUS-1B (Vp-1B) with STS molecular mark... The core collection of red-seeded winter hexaploid triticale with different pre-harvest sprouting (PHS) resistance has been evaluated for the allelic structure of the gene VIVIPAROUS-1B (Vp-1B) with STS molecular marker. The discovered structure of the collection was as follows: 50.0% and 41.7% of the collection carries Vp-1Bа and Vp-1Bc alleles, respectively, while 8,3% possesses both of them. As a result of the seed color estimation, the collection has been divided into two groups: with dark red seeds and light red seeds. The allele Vp-1Bc has appeared to be associated with PHS resistance while Vp-1Ba with PHS susceptibility in the triticale accessions with light red seeds only. The influence of the seed color and allelic state of Vp-1B on PHS resistance in triticale is discussed. 展开更多
关键词 Preharvest sprouting (PHS) HEXAPLOID TRITICALE VIVIPAROUS-1B Seed Color
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Acrylamide inhibits nerve sprouting induced by botulinum toxin type A
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作者 Hong Jiang Yi Xiang +1 位作者 Xingyue Hu Huaying Cai 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第16期1525-1531,共7页
Botulinum toxin type A is a potent muscle relaxant that blocks the transmission and release of acetylcholine at the neuromuscular junction. Intramuscular injection of botulinum toxin type A has served as an effective ... Botulinum toxin type A is a potent muscle relaxant that blocks the transmission and release of acetylcholine at the neuromuscular junction. Intramuscular injection of botulinum toxin type A has served as an effective and safe therapy for strabismus and focal dystonia. However, muscular weakness is temporary and after 3-4 months, muscle strength usually recovers because function- al recovery is mediated by nerve sprouting and reconstruction of the neuromuscular junction. Acrylamide may produce neurotoxic substances that cause retrograde necrotizing neuropathy and inhibit nerve sprouting caused by botulinum toxin type A. This study investigated whether acrylamide inhibits nerve sprouting after intramuscular injection of botulinum toxin type A. A tibial nerve sprouting model was established through local injection of botulinum toxin type A into the right gastrocnemius muscle of Sprague-Dawley rats. Following intramuscular injection, rats were given intraperitoneal injection of 3% acrylamide every 3 days for 21 days. Nerve sprout- ing appeared 2 weeks after intramuscular injection of botulinum toxin type A and single-fiber electromyography revealed abnormal conduction at the neuromuscular junction I week after intra- muscular injection of botulinum toxin type A. Following intraperitoneal injection of acrylamide, the peak muscle fiber density decreased. Electromyography jitter value were restored to normal levels 6 weeks after injection. This indicates that the maximal decrease in fiber density and the time at which functional conduction of neuromuscular junction was restored were delayed. Addition- ally, the increase in tibial nerve fibers was reduced. Acrylamide inhibits nerve sprouting caused by botulinum toxin type A and may be used to prolong the clinical dosage of botulinum toxin type A. 展开更多
关键词 nerve regeneration peripheral nerve regeneration botulinum toxin type A ACRYLAMIDE nerve sprouting ELECTROMYOGRAPHY nerve fibers neuromuscular junction single-fiber EMG fiberdensity action potential mean consecutive difference dysmyotonia neural regeneration
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Repairing the injured spinal cord: sprouting versus regeneration. Is this a realistic match?
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作者 Karim Fouad Caitlin Hurd 《Neural Regeneration Research》 SCIE CAS CSCD 2014年第5期462-462,共1页
The article by Meves and Zheng (2014) is addressing a continu- ous shift in the field of spinal cord injury (SCI) research that has occurred over the last century. Before that, the spinal cord was viewed as "hard... The article by Meves and Zheng (2014) is addressing a continu- ous shift in the field of spinal cord injury (SCI) research that has occurred over the last century. Before that, the spinal cord was viewed as "hard wired" and treatment considerations were based on observations that axons in the periphery were able to regenerate, but those in the central nervous system (CNS) were not (David and Aguayo, 1981). 展开更多
关键词 this sprouting versus regeneration SCI Repairing the injured spinal cord Is this a realistic match
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Sprouting Value Index and Viability Percentage: An Integrated Measure of Vegetative Planting Material
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作者 Justin R. Nayagam 《Journal of Agricultural Science and Technology(B)》 2016年第5期330-334,共5页
Rooting ability of indole 3-butyric acid (IBA)-induced stem cuttings in vegetative regeneration experiments depends on various parameters, which can be entrenched using sprouting value index (SVI), a mathematical ... Rooting ability of indole 3-butyric acid (IBA)-induced stem cuttings in vegetative regeneration experiments depends on various parameters, which can be entrenched using sprouting value index (SVI), a mathematical approach. In this study, field studies were carried out in Tabebuia rosea to generate clonal planting stock regeneration using three rooting medium at a location in Central Kerala, Peninsular India, for three consecutive years from October 2014 till June 2016 at four months regular intervals. Three IBA concentrations, viz., 200, 500 and 1,000 ppm, have been used and the data obtained was evaluated using SVI method in order to establish the suitable medium, which gives the maximum rooting results. Control cuttings did not record rooting in any of the trials. The high SVI was obtained when stem root cuttings planted in root trainers with coir pith compost (RTCP) for all the three concentration of IBA applied. Sprouting percentage was marked up and the delay in completion of sprouting/rooting initiation decreased by the use of IBA treatment. 展开更多
关键词 Pink trumpet tree sprouting value index rooting ability indole 3-butyric acid.
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