The main purpose of nonlinear time series analysis is based on the rebuilding theory of phase space, and to study how to transform the response signal to rebuilt phase space in order to extract dynamic feature informa...The main purpose of nonlinear time series analysis is based on the rebuilding theory of phase space, and to study how to transform the response signal to rebuilt phase space in order to extract dynamic feature information, and to provide effective approach for nonlinear signal analysis and fault diagnosis of nonlinear dynamic system. Now, it has already formed an important offset of nonlinear science. But, traditional method cannot extract chaos features automatically, and it needs man＇s participation in the whole process. A new method is put forward, which can implement auto-extracting of chaos features for nonlinear time series. Firstly, to confirm time delay r by autocorrelation method; Secondly, to compute embedded dimension m and correlation dimension D; Thirdly, to compute the maximum Lyapunov index λmax; Finally, to calculate the chaos degree Dch of Poincare map, and the non-circle degree Dnc and non-order degree Dno of quasi-phase orbit. Chaos features extracting has important meaning to fault diagnosis of nonlinear system based on nonlinear chaos features. Examples show validity of the proposed method.展开更多
AIM: To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA. METHODS: Three standards were prepared by cloning PCR products which targeted S, ...AIM: To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA. METHODS: Three standards were prepared by cloning PCR products which targeted S, C and X region of HBV genome into pGEM-T vector respectively. A pair of primers and matched TaqMan probe were selected by comparing the copy number and the Ct values of HBV serum samples derived from the three different standard curves using certain serum DNA. Then the efficiency of six HBV DNA extraction methods including guanidinium isothiocyanate, proteinase K, NaI, NaOH lysis, alkaline lysis and simple boiling was analyzed in sample A, B and C by real-time PCR. Meanwhile, 8 clinical HBV serum samples were quantified. RESULTS: The copy number of the same HBV serum sample originated from the standard curve of S, C and X regions was 5.7 × 104/mL, 6.3 × 102/mL and 1.6 × 103/ mL respectively. The relative Ct value was 26.6, 31.8 and 29.5 respectively. Therefore, primers and matched probe from S region were chosen for further optimization of six extraction methods. The copy number of HBV serum samples A, B and C was 3.49 × 109/mL, 2.08 × 106/mL and 4.40 × 107/mL respectively, the relative Ct valuewas 19.9, 30 and 26.2 in the method of NaOH lysis, which was the efficientest among six methods. Simple boiling showed a slightly lower efficiency than NaOH lysis. Guanidinium isothiocyanate, proteinase K and NaI displayed that the copy number of HBV serum sample A, B and C was around 105/mL, meanwhile the Ct value was about 30. Alkaline failed to quantify the copy number of three HBV serum samples. Standard deviation (SD) and coefficient variation (CV) were very low in all 8 clinical HBV serum samples, showing that quantification of HBV DNA in triplicate was reliable and accurate. CONCLUSION: Real-time PCR based on optimized primers and TaqMan probe from S region in combination with NaOH lysis is a simple, rapid and accurate method for quantification of HBV serum DNA.展开更多
An effective approach is presented to extract welds from real-time radiographs, Firstly an algorithm based on an adaptive bidirectional threshold was proposed to segment the gradient image into ternary image, and then...An effective approach is presented to extract welds from real-time radiographs, Firstly an algorithm based on an adaptive bidirectional threshold was proposed to segment the gradient image into ternary image, and then the bidirectional accumulator Hough Transform was developed to extract weld edges from the ternary image. Different values of the coefficient proposed in the threshold algorithm were tested, and the proposed approach was applied to extract welds from real-time radiographic images of different types of welds with defects. Results show that the proposed method is adaptive and effective to extract welds from real-time radiographs of linear welds.展开更多
校核、验证与确认(verification,validation,and accreditation,VV&A)是保证仿真模型可信度的关键手段,其中模型验证是核心环节。针对导弹飞行仿真模型结果验证存在的参考数据不可获得、参考数据来源多样、专家验证主观性强等问题,...校核、验证与确认(verification,validation,and accreditation,VV&A)是保证仿真模型可信度的关键手段,其中模型验证是核心环节。针对导弹飞行仿真模型结果验证存在的参考数据不可获得、参考数据来源多样、专家验证主观性强等问题,提出一种基于时间序列分段特征提取的导弹飞行仿真模型结果验证方法。提出了一种综合的时间序列分段线性方法,由基于二阶导数提取趋势边缘点的线性分段算法和基于极值点优化的Top-Down线性分段算法两部分组成,以实现对导弹飞行仿真数据和参考数据进行有效的线性分段表示。基于上述分段结果,对各段时间序列的均值、方差、斜率等特征进行提取,以辅助专家进行验证,从而降低验证中的主观性;或者直接利用TIC系数法、动态时间规整(dynamic time warping,DTW)等方法进行客观的相似性分析。通过充分利用时间序列的分段特征,可实现各种情形下的导弹飞行仿真模型结果验证。通过一个导弹模型结果验证案例演示了所提方法的可行性和有效性。展开更多
利用磁珠法对甘蓝、白菜、芥菜、洋葱、黄瓜、番茄、辣椒等7种形状、大小不同的蔬菜单粒种子和幼苗进行DNA提取,采用内参基因Actin进行实时荧光定量PCR评价DNA质量,利用KASP(kompetitive allele specific PCR)标记检验DNA质量是否达到K...利用磁珠法对甘蓝、白菜、芥菜、洋葱、黄瓜、番茄、辣椒等7种形状、大小不同的蔬菜单粒种子和幼苗进行DNA提取,采用内参基因Actin进行实时荧光定量PCR评价DNA质量,利用KASP(kompetitive allele specific PCR)标记检验DNA质量是否达到KASP检测要求。结果显示,单粒种子磁珠法提取的各蔬菜DNA平均Ct值均处于19~24范围内。KASP检测进一步证实种子提取DNA的质量达到KASP标记分型的要求。综上所述,本研究建立了一种简单、经济、实用性强的单粒种子DNA提取方法,该方法对不同大小的蔬菜种子具有较好的稳定性,且大幅缩短了获得基因型数据的时间。展开更多
文摘The main purpose of nonlinear time series analysis is based on the rebuilding theory of phase space, and to study how to transform the response signal to rebuilt phase space in order to extract dynamic feature information, and to provide effective approach for nonlinear signal analysis and fault diagnosis of nonlinear dynamic system. Now, it has already formed an important offset of nonlinear science. But, traditional method cannot extract chaos features automatically, and it needs man＇s participation in the whole process. A new method is put forward, which can implement auto-extracting of chaos features for nonlinear time series. Firstly, to confirm time delay r by autocorrelation method; Secondly, to compute embedded dimension m and correlation dimension D; Thirdly, to compute the maximum Lyapunov index λmax; Finally, to calculate the chaos degree Dch of Poincare map, and the non-circle degree Dnc and non-order degree Dno of quasi-phase orbit. Chaos features extracting has important meaning to fault diagnosis of nonlinear system based on nonlinear chaos features. Examples show validity of the proposed method.
基金Supported by the National Natural Science Foundation of China(No. 30371328), the Key Project of Natural Science Foundationof Shandong Province (No. Z2002C01), and the Key Project ofShandong Academy of Medical Sciences (No. 2005007)
文摘AIM: To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA. METHODS: Three standards were prepared by cloning PCR products which targeted S, C and X region of HBV genome into pGEM-T vector respectively. A pair of primers and matched TaqMan probe were selected by comparing the copy number and the Ct values of HBV serum samples derived from the three different standard curves using certain serum DNA. Then the efficiency of six HBV DNA extraction methods including guanidinium isothiocyanate, proteinase K, NaI, NaOH lysis, alkaline lysis and simple boiling was analyzed in sample A, B and C by real-time PCR. Meanwhile, 8 clinical HBV serum samples were quantified. RESULTS: The copy number of the same HBV serum sample originated from the standard curve of S, C and X regions was 5.7 × 104/mL, 6.3 × 102/mL and 1.6 × 103/ mL respectively. The relative Ct value was 26.6, 31.8 and 29.5 respectively. Therefore, primers and matched probe from S region were chosen for further optimization of six extraction methods. The copy number of HBV serum samples A, B and C was 3.49 × 109/mL, 2.08 × 106/mL and 4.40 × 107/mL respectively, the relative Ct valuewas 19.9, 30 and 26.2 in the method of NaOH lysis, which was the efficientest among six methods. Simple boiling showed a slightly lower efficiency than NaOH lysis. Guanidinium isothiocyanate, proteinase K and NaI displayed that the copy number of HBV serum sample A, B and C was around 105/mL, meanwhile the Ct value was about 30. Alkaline failed to quantify the copy number of three HBV serum samples. Standard deviation (SD) and coefficient variation (CV) were very low in all 8 clinical HBV serum samples, showing that quantification of HBV DNA in triplicate was reliable and accurate. CONCLUSION: Real-time PCR based on optimized primers and TaqMan probe from S region in combination with NaOH lysis is a simple, rapid and accurate method for quantification of HBV serum DNA.
文摘An effective approach is presented to extract welds from real-time radiographs, Firstly an algorithm based on an adaptive bidirectional threshold was proposed to segment the gradient image into ternary image, and then the bidirectional accumulator Hough Transform was developed to extract weld edges from the ternary image. Different values of the coefficient proposed in the threshold algorithm were tested, and the proposed approach was applied to extract welds from real-time radiographic images of different types of welds with defects. Results show that the proposed method is adaptive and effective to extract welds from real-time radiographs of linear welds.
文摘校核、验证与确认(verification,validation,and accreditation,VV&A)是保证仿真模型可信度的关键手段,其中模型验证是核心环节。针对导弹飞行仿真模型结果验证存在的参考数据不可获得、参考数据来源多样、专家验证主观性强等问题,提出一种基于时间序列分段特征提取的导弹飞行仿真模型结果验证方法。提出了一种综合的时间序列分段线性方法,由基于二阶导数提取趋势边缘点的线性分段算法和基于极值点优化的Top-Down线性分段算法两部分组成,以实现对导弹飞行仿真数据和参考数据进行有效的线性分段表示。基于上述分段结果,对各段时间序列的均值、方差、斜率等特征进行提取,以辅助专家进行验证,从而降低验证中的主观性;或者直接利用TIC系数法、动态时间规整(dynamic time warping,DTW)等方法进行客观的相似性分析。通过充分利用时间序列的分段特征,可实现各种情形下的导弹飞行仿真模型结果验证。通过一个导弹模型结果验证案例演示了所提方法的可行性和有效性。
文摘利用磁珠法对甘蓝、白菜、芥菜、洋葱、黄瓜、番茄、辣椒等7种形状、大小不同的蔬菜单粒种子和幼苗进行DNA提取,采用内参基因Actin进行实时荧光定量PCR评价DNA质量,利用KASP(kompetitive allele specific PCR)标记检验DNA质量是否达到KASP检测要求。结果显示,单粒种子磁珠法提取的各蔬菜DNA平均Ct值均处于19~24范围内。KASP检测进一步证实种子提取DNA的质量达到KASP标记分型的要求。综上所述,本研究建立了一种简单、经济、实用性强的单粒种子DNA提取方法,该方法对不同大小的蔬菜种子具有较好的稳定性,且大幅缩短了获得基因型数据的时间。
