2-(pyridine-2-yl)-N-p-chlorohydrazinecarbothioamide (HCPTS) was synthesized, characterized and successfully applied for the preconcentration of Cu(II), Ni(II), Zn(II), Cd(II), Co(II), Pb(II), Fe(II), and Hg(II) in wat...2-(pyridine-2-yl)-N-p-chlorohydrazinecarbothioamide (HCPTS) was synthesized, characterized and successfully applied for the preconcentration of Cu(II), Ni(II), Zn(II), Cd(II), Co(II), Pb(II), Fe(II), and Hg(II) in water, blood, and urine samples prior to graphite furnace atomic absorption determination (GFAAS);Hg was determined by cold vapor technique. Under the optimum experimental conditions (i.e. pH = 8, 10–4 M of HCPTS, 0.05% w/v of Triton X-114), calibration graphs were linear in the range of 0.02 to 200 ng?mL–1 for Co(II), Cd(II), Pb(II) and Ni(II);0.03 to 200 ng?mL–1 for Cu(II);0.07 to 200 ng?mL–1 for Fe(II) and Zn(II) and 0.02 to 150 ng?mL–1 for Hg(II). The enrichment factors were 43, 51, 41, 46, 54, 40, 45 and 52 for Cu(II), Ni(II),Zn (II), Cd(II), Co(II), Pb(II), Fe(II), and Hg(II), respectively. The limit of detection were found to be 0.019, 0.094, 0.0514, 0.052, 0.0165, 0.047, 0.068 and 0.041 ng?mL–1 for Cu(II), Ni(II), Zn(II), Cd(II), Co(II), Pb(II), Fe(II), and Hg(II), respectively. The developed method was applied to the determination of these metal ions in water, blood and urine samples with satisfactory results.展开更多
Silica gel-loaded (E)-N-(1-thien-2'-ylethylidene)-1,2-phenylenediamine (TEPDA) phase was synthesized based on physical adsorption approaches. The stability of a chemically modified TEPDA especially in concentra...Silica gel-loaded (E)-N-(1-thien-2'-ylethylidene)-1,2-phenylenediamine (TEPDA) phase was synthesized based on physical adsorption approaches. The stability of a chemically modified TEPDA especially in concentrated hydrochloric acid that was then used as a recycling and preconcentration reagent allowed the further uses of silica gel-loaded immobilized TEPDA phase. The application of this silica gel-loaded phase to sorption of a series of metal ions was performed by using different controlling factors such as the pH of the metal ion solution and the equilibration shaking time by the static technique. This difference was interpreted on the basis of selectivity incorporated in these sulfur containing silica gel-loaded TEPDA phases. Hg(Ⅱ) was found to exhibit the highest affinity towards extraction by these silica gel-loaded TEPDA phases. The pronounced selectivity was also confirmed by the determined distribution coefficients (Kd) of all the metal ions, showing the highest value reported for mercury(Ⅱ) extraction by the silica gel immobilized TEPDA phase. The potential applications of the silica gel immobilized TEPDA phase to selective extraction of mercury(Ⅱ) from aqueous solution were successfully accomplished and preconcentration of low concentration of Hg(Ⅱ) (30 pg·mL^-1) from natural tap water with a preconcentration factor of 200 for Hg(Ⅱ) off-line analysis was conducted by cold vapor atomic absorption analysis.展开更多
In order to clarify the mechanism and the complex formed in the extraction of divalent Mn by alkylphosphonic acid monoester, the solid complex has been prepared by mono (2-ethylhexyl)-2-ethylhexyl phosphate, HEH(EH)P,...In order to clarify the mechanism and the complex formed in the extraction of divalent Mn by alkylphosphonic acid monoester, the solid complex has been prepared by mono (2-ethylhexyl)-2-ethylhexyl phosphate, HEH(EH)P, HA with divalent Mn. The elemental analysis, magnetic susceptibility and thermogravimetry have been determined for the solid complex of HEH(EH)P-Mn(II) and the infrared spectrum has been carried out in comparison to the extractant HEH(EH)P. The extracted compound has also been studied by electronic and electron spin resonance spectroscopy in octane solvent and solid state at room temperature. On the basis of the measurements, it is concluded that the structure of the solid polymeric species MnA_2 is in a tetrahedral arrangement.展开更多
This study is designed to evaluate antioxidant and antigenotoxic activities of corn tassel extracts(CTTs). The major bioactive components of CTTs include flavonoid, saponin and polysaccharide. The antioxidant proper...This study is designed to evaluate antioxidant and antigenotoxic activities of corn tassel extracts(CTTs). The major bioactive components of CTTs include flavonoid, saponin and polysaccharide. The antioxidant properties of the three bioactive components of CTTs were investigated by Ferric Reducing Antioxidant Property(FRAP) and 1, 1-diphenyl-2-picrylhydrazyl(DPPH) assays. The activities of the extracts were determined by assessing the inhibition of mutagenicity of the direct-acting mutagen fenaminosulf, sodium azide, and indirect-acting mutagen 2-aminofluorene using the Ames test(strains TA98 and TA100). The results showed that the extraction rates of flavonoid, saponin, and polysaccharide from the dried corn tassels were 1.67%, 2.41% and 4.76% respectively. DPPH and FRAP assay strongly demonstrated that CTTs had antioxidant properties. CTTs at doses of 625, 1250 and 2500 μg per plate reduced 2-aminofluorene mutagenicity by 12.52%, 28.76% and 36.49% in Salmonella typhimurium TA98 strain assay respectively and by 10.98%, 25.27% and 37.83%, at the same doses in Salmonella typhimurium TA100 assay system, respectively. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide(MTT) assay showed that the different concentrations of CTTs inhibited the proliferation of MGC80-3 cells in a dose-dependent manner(P0.01). It is concluded that these integrated approaches to antioxidant and antigenotoxicity assessment may be useful to study corn tassel as a natural herbal material.展开更多
Objective: Marsdenia tenacissima extract(MTE) is a traditional Chinese herbal medicine with anti-cancer activity. In some previous studies, different mechanism actions of the anti-cancer effect of MTE have been reveal...Objective: Marsdenia tenacissima extract(MTE) is a traditional Chinese herbal medicine with anti-cancer activity. In some previous studies, different mechanism actions of the anti-cancer effect of MTE have been revealed. In this study, we first observed that MTE exhibited G2/M cell cycle arrest on two different human breast cancer cell lines, MDA-MB-231 and MCF-7 by mediating 14-3-3σ and c-myc.Methods: The effect of MTE on G2/M cell cycle arrest was evaluated in MDA-MB-231 and MCF-7 cell lines. MTT assay was done for evaluation of cell viability. Flow cytometry was employed for cell cycle analysis. Western blotting analysis and immunohistochemistry were performed to analyze the expression of G2/M cell cycle-related key protein in cells and tissue samples. Animal studies have been conducted to elucidate the anti-tumor effect of MTE.Results: Cell cycle is the backbone for developing cancer. Cell cycle proteins play a major role in the progression of cell cycle and cell proliferation. However, some key protein directly or indirectly modulate the action of cell cycle protein that highly affect cell cycle regulation. In order to investigate cellular proliferation of cancer, we observed that MTE induced the upregulation of 14-3-3σ and downregulation of c-myc,and then reduced the expression of G2/M cell cycle associated key protein, leading to the inhibition of cellular entry into mitosis phase. We also confirmed that MTE exerted a significant antitumor effect on the MDA-MB-231 xenograft model in vivo.Conclusion: G2/M cell cycle arrest occurred by the action of MTE, mediated by the upregulation of 14-3-3σ as well as downregulation of c-myc in MDA-MB-231 and MCF-7 cell lines.展开更多
Separation of palladium(Pd)and platinum(Pt)by solvent extraction is difficult because of their extremely similar physicochemical properties.Development of new extractants with a high extraction efficiency and excellen...Separation of palladium(Pd)and platinum(Pt)by solvent extraction is difficult because of their extremely similar physicochemical properties.Development of new extractants with a high extraction efficiency and excellent separation selectivity is the primary focus for enhancing the separation of Pd and Pt by solvent extraction.In this study,a new extractant,2-((2-methoxyethyl)thio)-1 H-benzimidazole(MOBI),which contains N,S,and O atoms,was synthesized and used to separate Pd(Ⅱ)and Pt(Ⅳ)from a hydrochloric acid aqueous solution.The experimental results revealed that Pd(Ⅱ)and Pt(Ⅳ)were effectively separated under the optimal conditions:MOB I concentration of 0.005 mol·L^(-1),HCl concentration of0.2 mol·L^(-1),organic/aqueous(O/A)phase ratio of 1.0,and contact time of 15 min.The separation coefficient between Pd(Ⅱ)and Pt(Ⅳ)was 1246.40,which indicates that MOBI has a higher selectivity for Pd(Ⅱ)than Pt(Ⅳ).In addition,the mechanism of coordination between Pd(Ⅱ)and MOBI was demonstrated through the slope method,Fourier transform infrared spectroscopy(FTIR),hydrogen-1 nuclear magnetic resonance spectroscopy(^(1)H NMR),and carbon-13 nuclear magnetic resonance spectroscopy(^(13)C NMR).The results revealed that MOBI could coordinate with Pd(Ⅱ)via the N atom in the benzimidazole ring of MOBI molecules to form[Pd(MOBI)_(2)Cl_(2)](O).展开更多
The treatment of wastewaters is crucial to maintain the ecological status of receiving waters,and thereby guarantee the protection of aquatic life and human health.Wastewater quality evaluation is conventionally based...The treatment of wastewaters is crucial to maintain the ecological status of receiving waters,and thereby guarantee the protection of aquatic life and human health.Wastewater quality evaluation is conventionally based on physicochemical parameters,but increasing attention has been paid to integrate physicochemical and biological data.Nevertheless,the regulatory use of fish in biological testing methods has been subject to various ethical and cost concerns,and in vitro cell-based assays have thus become an important topic of interest.Hence,the present study intends:(a) to evaluate the efficiency of two different sample pre-concentration techniques (lyophilisation and solid phase extraction) to assess the toxicity of municipal effluents on rat cardiomyoblast H9c2(2-1) cells,and (b) maximizing the use of the effluent sample collected,to estimate the environmental condition of the receiving environment.The gathered results demonstrate that the H9c2(2-1) sulforhodamine B-based assay is an appropriate in vitro method to assess biological effluent toxicity,and the best results were attained by lyophilising the sample as pre-treatment.Due to its response,the H9c2(2-1) cell line might be a possible alternative in vitro model for fish lethal testing to assess the toxicity of municipal effluents.The physicochemical status of the sample suggests a high potential for eutrophication,and iron exceeded the permissible level for wastewater discharge,possibly due to the addition of ferric chloride for wastewater treatment.In general,the levels of carbamazepine and sulfamethoxazole are higher than those reported for other countries,and both surpassed the aquatic protective values for long-term exposure.展开更多
Preconcentration/separation of Co(II), Fe(III), Pb(II), Cr(IIl), Cu(II) and Cd(II) ions using bis(5-bromo- 2-hydroxy-benzaldehyde)-2-methy1-1,5-pentane diimine (BBHBPDI) on SDS coated alumina has been ...Preconcentration/separation of Co(II), Fe(III), Pb(II), Cr(IIl), Cu(II) and Cd(II) ions using bis(5-bromo- 2-hydroxy-benzaldehyde)-2-methy1-1,5-pentane diimine (BBHBPDI) on SDS coated alumina has been reported. The influences of the analytical parameters including pH, ligand and SDS amount, type and concentration of eluent and sample volume on metal ions recoveries were investigated. At optimum values of all variables the relative standard deviation are between 2.5-2.7 and preconcentration factor was 375, while recoveries for all understudy metal ions are higher than 95%, determination limits are between 1.5-2.7. The method has been successfully applied to determination of Co(II), Fe(III), Pb(II), Cr(III), Cu(II) and Cd(II) ions content in some real samples.展开更多
In order to Isolate anti-human Immunodeflclency virus (HIV) agents from natural products, 97 ethanollc extracts of 90 fungi were tested for their Inhibitory activity on HIV-1. Most of the extracts tested were relati...In order to Isolate anti-human Immunodeflclency virus (HIV) agents from natural products, 97 ethanollc extracts of 90 fungi were tested for their Inhibitory activity on HIV-1. Most of the extracts tested were relatively non-toxic to human lymphocytic MT-4 cells, but extracts of some fungi exhibited potent antl-HIV activity In an in vitro 3-(4, 5-dlmethyl-2 thlazoyl)-2,5-dlphenyl-2H-tetrazollum bromide assay with a selectivity Index greater than 3. Most fungi were Isolated from Dendrobium sp. and Taxus sp.展开更多
文摘2-(pyridine-2-yl)-N-p-chlorohydrazinecarbothioamide (HCPTS) was synthesized, characterized and successfully applied for the preconcentration of Cu(II), Ni(II), Zn(II), Cd(II), Co(II), Pb(II), Fe(II), and Hg(II) in water, blood, and urine samples prior to graphite furnace atomic absorption determination (GFAAS);Hg was determined by cold vapor technique. Under the optimum experimental conditions (i.e. pH = 8, 10–4 M of HCPTS, 0.05% w/v of Triton X-114), calibration graphs were linear in the range of 0.02 to 200 ng?mL–1 for Co(II), Cd(II), Pb(II) and Ni(II);0.03 to 200 ng?mL–1 for Cu(II);0.07 to 200 ng?mL–1 for Fe(II) and Zn(II) and 0.02 to 150 ng?mL–1 for Hg(II). The enrichment factors were 43, 51, 41, 46, 54, 40, 45 and 52 for Cu(II), Ni(II),Zn (II), Cd(II), Co(II), Pb(II), Fe(II), and Hg(II), respectively. The limit of detection were found to be 0.019, 0.094, 0.0514, 0.052, 0.0165, 0.047, 0.068 and 0.041 ng?mL–1 for Cu(II), Ni(II), Zn(II), Cd(II), Co(II), Pb(II), Fe(II), and Hg(II), respectively. The developed method was applied to the determination of these metal ions in water, blood and urine samples with satisfactory results.
基金Project supported by the National Natural Science Foundation of China (No. 20271025), the Natural Science Foundation of Shandong Province (No. L2003B01) and the State Key Laboratory of Crystal Materials, Shandong University.
文摘Silica gel-loaded (E)-N-(1-thien-2'-ylethylidene)-1,2-phenylenediamine (TEPDA) phase was synthesized based on physical adsorption approaches. The stability of a chemically modified TEPDA especially in concentrated hydrochloric acid that was then used as a recycling and preconcentration reagent allowed the further uses of silica gel-loaded immobilized TEPDA phase. The application of this silica gel-loaded phase to sorption of a series of metal ions was performed by using different controlling factors such as the pH of the metal ion solution and the equilibration shaking time by the static technique. This difference was interpreted on the basis of selectivity incorporated in these sulfur containing silica gel-loaded TEPDA phases. Hg(Ⅱ) was found to exhibit the highest affinity towards extraction by these silica gel-loaded TEPDA phases. The pronounced selectivity was also confirmed by the determined distribution coefficients (Kd) of all the metal ions, showing the highest value reported for mercury(Ⅱ) extraction by the silica gel immobilized TEPDA phase. The potential applications of the silica gel immobilized TEPDA phase to selective extraction of mercury(Ⅱ) from aqueous solution were successfully accomplished and preconcentration of low concentration of Hg(Ⅱ) (30 pg·mL^-1) from natural tap water with a preconcentration factor of 200 for Hg(Ⅱ) off-line analysis was conducted by cold vapor atomic absorption analysis.
文摘In order to clarify the mechanism and the complex formed in the extraction of divalent Mn by alkylphosphonic acid monoester, the solid complex has been prepared by mono (2-ethylhexyl)-2-ethylhexyl phosphate, HEH(EH)P, HA with divalent Mn. The elemental analysis, magnetic susceptibility and thermogravimetry have been determined for the solid complex of HEH(EH)P-Mn(II) and the infrared spectrum has been carried out in comparison to the extractant HEH(EH)P. The extracted compound has also been studied by electronic and electron spin resonance spectroscopy in octane solvent and solid state at room temperature. On the basis of the measurements, it is concluded that the structure of the solid polymeric species MnA_2 is in a tetrahedral arrangement.
基金supported by the National Natural Science Foundation of China(No.21077082)
文摘This study is designed to evaluate antioxidant and antigenotoxic activities of corn tassel extracts(CTTs). The major bioactive components of CTTs include flavonoid, saponin and polysaccharide. The antioxidant properties of the three bioactive components of CTTs were investigated by Ferric Reducing Antioxidant Property(FRAP) and 1, 1-diphenyl-2-picrylhydrazyl(DPPH) assays. The activities of the extracts were determined by assessing the inhibition of mutagenicity of the direct-acting mutagen fenaminosulf, sodium azide, and indirect-acting mutagen 2-aminofluorene using the Ames test(strains TA98 and TA100). The results showed that the extraction rates of flavonoid, saponin, and polysaccharide from the dried corn tassels were 1.67%, 2.41% and 4.76% respectively. DPPH and FRAP assay strongly demonstrated that CTTs had antioxidant properties. CTTs at doses of 625, 1250 and 2500 μg per plate reduced 2-aminofluorene mutagenicity by 12.52%, 28.76% and 36.49% in Salmonella typhimurium TA98 strain assay respectively and by 10.98%, 25.27% and 37.83%, at the same doses in Salmonella typhimurium TA100 assay system, respectively. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide(MTT) assay showed that the different concentrations of CTTs inhibited the proliferation of MGC80-3 cells in a dose-dependent manner(P0.01). It is concluded that these integrated approaches to antioxidant and antigenotoxicity assessment may be useful to study corn tassel as a natural herbal material.
基金supported by Nanjing Sanhome Pharmaceutical Co., Ltd
文摘Objective: Marsdenia tenacissima extract(MTE) is a traditional Chinese herbal medicine with anti-cancer activity. In some previous studies, different mechanism actions of the anti-cancer effect of MTE have been revealed. In this study, we first observed that MTE exhibited G2/M cell cycle arrest on two different human breast cancer cell lines, MDA-MB-231 and MCF-7 by mediating 14-3-3σ and c-myc.Methods: The effect of MTE on G2/M cell cycle arrest was evaluated in MDA-MB-231 and MCF-7 cell lines. MTT assay was done for evaluation of cell viability. Flow cytometry was employed for cell cycle analysis. Western blotting analysis and immunohistochemistry were performed to analyze the expression of G2/M cell cycle-related key protein in cells and tissue samples. Animal studies have been conducted to elucidate the anti-tumor effect of MTE.Results: Cell cycle is the backbone for developing cancer. Cell cycle proteins play a major role in the progression of cell cycle and cell proliferation. However, some key protein directly or indirectly modulate the action of cell cycle protein that highly affect cell cycle regulation. In order to investigate cellular proliferation of cancer, we observed that MTE induced the upregulation of 14-3-3σ and downregulation of c-myc,and then reduced the expression of G2/M cell cycle associated key protein, leading to the inhibition of cellular entry into mitosis phase. We also confirmed that MTE exerted a significant antitumor effect on the MDA-MB-231 xenograft model in vivo.Conclusion: G2/M cell cycle arrest occurred by the action of MTE, mediated by the upregulation of 14-3-3σ as well as downregulation of c-myc in MDA-MB-231 and MCF-7 cell lines.
基金the National Natural Science Foundation of China(Nos.51362012,51662007,51574213,and U1602273)Yunnan Applied BasicResearch Project(Nos.2017FD157 and 2018FH001-049)Yunnan Province Department of Education Fund(No.2019J1183)。
文摘Separation of palladium(Pd)and platinum(Pt)by solvent extraction is difficult because of their extremely similar physicochemical properties.Development of new extractants with a high extraction efficiency and excellent separation selectivity is the primary focus for enhancing the separation of Pd and Pt by solvent extraction.In this study,a new extractant,2-((2-methoxyethyl)thio)-1 H-benzimidazole(MOBI),which contains N,S,and O atoms,was synthesized and used to separate Pd(Ⅱ)and Pt(Ⅳ)from a hydrochloric acid aqueous solution.The experimental results revealed that Pd(Ⅱ)and Pt(Ⅳ)were effectively separated under the optimal conditions:MOB I concentration of 0.005 mol·L^(-1),HCl concentration of0.2 mol·L^(-1),organic/aqueous(O/A)phase ratio of 1.0,and contact time of 15 min.The separation coefficient between Pd(Ⅱ)and Pt(Ⅳ)was 1246.40,which indicates that MOBI has a higher selectivity for Pd(Ⅱ)than Pt(Ⅳ).In addition,the mechanism of coordination between Pd(Ⅱ)and MOBI was demonstrated through the slope method,Fourier transform infrared spectroscopy(FTIR),hydrogen-1 nuclear magnetic resonance spectroscopy(^(1)H NMR),and carbon-13 nuclear magnetic resonance spectroscopy(^(13)C NMR).The results revealed that MOBI could coordinate with Pd(Ⅱ)via the N atom in the benzimidazole ring of MOBI molecules to form[Pd(MOBI)_(2)Cl_(2)](O).
基金The Portuguese Foundation for Science and Technology (FCT) supported the present study through a fellowship attributed to Elsa Teresa Rodrigues(SFRH/BPD/116152/2016),which was funded by the Human Potential Operating Program of the European Social Fund,and by the Portuguese budget through the Ministry of Education and Sciencedeveloped within the Fish Free Project(PTDC/AAG-TEC/4966/2014)+3 种基金supported by the FCT through national funds (3599-PPCDT)the co-funding of the European Regional Development Fund (POCI-01-0145-FEDER-016875)supported within the PT2020 Partnership Agreement and COMPETE 2020 by the centre for Functional Ecology Strategic Project (UID/BIA/04004/2013)the Coimbra Chemistry Center Strategic Project (UID/QUI/00313/2013)。
文摘The treatment of wastewaters is crucial to maintain the ecological status of receiving waters,and thereby guarantee the protection of aquatic life and human health.Wastewater quality evaluation is conventionally based on physicochemical parameters,but increasing attention has been paid to integrate physicochemical and biological data.Nevertheless,the regulatory use of fish in biological testing methods has been subject to various ethical and cost concerns,and in vitro cell-based assays have thus become an important topic of interest.Hence,the present study intends:(a) to evaluate the efficiency of two different sample pre-concentration techniques (lyophilisation and solid phase extraction) to assess the toxicity of municipal effluents on rat cardiomyoblast H9c2(2-1) cells,and (b) maximizing the use of the effluent sample collected,to estimate the environmental condition of the receiving environment.The gathered results demonstrate that the H9c2(2-1) sulforhodamine B-based assay is an appropriate in vitro method to assess biological effluent toxicity,and the best results were attained by lyophilising the sample as pre-treatment.Due to its response,the H9c2(2-1) cell line might be a possible alternative in vitro model for fish lethal testing to assess the toxicity of municipal effluents.The physicochemical status of the sample suggests a high potential for eutrophication,and iron exceeded the permissible level for wastewater discharge,possibly due to the addition of ferric chloride for wastewater treatment.In general,the levels of carbamazepine and sulfamethoxazole are higher than those reported for other countries,and both surpassed the aquatic protective values for long-term exposure.
文摘Preconcentration/separation of Co(II), Fe(III), Pb(II), Cr(IIl), Cu(II) and Cd(II) ions using bis(5-bromo- 2-hydroxy-benzaldehyde)-2-methy1-1,5-pentane diimine (BBHBPDI) on SDS coated alumina has been reported. The influences of the analytical parameters including pH, ligand and SDS amount, type and concentration of eluent and sample volume on metal ions recoveries were investigated. At optimum values of all variables the relative standard deviation are between 2.5-2.7 and preconcentration factor was 375, while recoveries for all understudy metal ions are higher than 95%, determination limits are between 1.5-2.7. The method has been successfully applied to determination of Co(II), Fe(III), Pb(II), Cr(III), Cu(II) and Cd(II) ions content in some real samples.
基金National Medicine Science Foundation during the 10th Five-Year Plan Period (2001BA705B01) and the National Science Fund for Distinguished Young Scholars (30325047).
文摘In order to Isolate anti-human Immunodeflclency virus (HIV) agents from natural products, 97 ethanollc extracts of 90 fungi were tested for their Inhibitory activity on HIV-1. Most of the extracts tested were relatively non-toxic to human lymphocytic MT-4 cells, but extracts of some fungi exhibited potent antl-HIV activity In an in vitro 3-(4, 5-dlmethyl-2 thlazoyl)-2,5-dlphenyl-2H-tetrazollum bromide assay with a selectivity Index greater than 3. Most fungi were Isolated from Dendrobium sp. and Taxus sp.