Different nutrient media can affect in vitro culturing proto- cols, and experimentation under varied growth conditions is valuable in plants where in vitro methods are in preliminary stages. We carried out the first i...Different nutrient media can affect in vitro culturing proto- cols, and experimentation under varied growth conditions is valuable in plants where in vitro methods are in preliminary stages. We carried out the first in vitro propagation studies for the endangered species Caragana fruticosa （Fabaceae）. We evaluated various nulrient media for their im- pact on shoot elongation and axillary bud proliferation using different concentrations of 6-benzylaminopurine （BA） and n-naphthaleneacetic acid （NAA）. Shoot elongation was evaluated based on adventitious shoot primary culture and subculture regeneration from Caragana seedlings. Our goal was to improve both micropropagation and regeneration in C. fruticosa. MS nutrient media was superior to 1/2MS macronutrients, DKW, QL, and WPM for shoot elongation and axillary shoot prolifera- tion. Shoots grown on 1/2MS and WPM exhibited some chlorosis, and shoots on QL produced larger leavers than plants growing on normal medium. The shoot proliferation coefficient on MS media supplemented with 2.22 μM BA and 0.44μM BA ＋ 2.69 μM NAA was significantly higher than that with other treatments in the primary culture. Shoots on 2.22 μM BA showed a higher proliferation coefficient （3.17） than others in the subculture. Shoots were rooted on 1/2MS medium with the addition of different concentrations of NAA. The optimal concenWation for rooting was 0.27 μM NAA （74%）. Roots exhibited many stout and long root hairs. Survivl of established plentlets was 82% at 30 days after transfer to soil. Plants established in the green house showed normal growth and displayed no apparent morphological differences compared to stock plants.展开更多
基金supported by the Key Technologies R&D Program of China during 2006-2010 (2006BAD03A04)the Fundamental Research Funds for the Central Universities(DL10BA04)
文摘Different nutrient media can affect in vitro culturing proto- cols, and experimentation under varied growth conditions is valuable in plants where in vitro methods are in preliminary stages. We carried out the first in vitro propagation studies for the endangered species Caragana fruticosa （Fabaceae）. We evaluated various nulrient media for their im- pact on shoot elongation and axillary bud proliferation using different concentrations of 6-benzylaminopurine （BA） and n-naphthaleneacetic acid （NAA）. Shoot elongation was evaluated based on adventitious shoot primary culture and subculture regeneration from Caragana seedlings. Our goal was to improve both micropropagation and regeneration in C. fruticosa. MS nutrient media was superior to 1/2MS macronutrients, DKW, QL, and WPM for shoot elongation and axillary shoot prolifera- tion. Shoots grown on 1/2MS and WPM exhibited some chlorosis, and shoots on QL produced larger leavers than plants growing on normal medium. The shoot proliferation coefficient on MS media supplemented with 2.22 μM BA and 0.44μM BA ＋ 2.69 μM NAA was significantly higher than that with other treatments in the primary culture. Shoots on 2.22 μM BA showed a higher proliferation coefficient （3.17） than others in the subculture. Shoots were rooted on 1/2MS medium with the addition of different concentrations of NAA. The optimal concenWation for rooting was 0.27 μM NAA （74%）. Roots exhibited many stout and long root hairs. Survivl of established plentlets was 82% at 30 days after transfer to soil. Plants established in the green house showed normal growth and displayed no apparent morphological differences compared to stock plants.
