Effects of Intestinal Trefoil Factor on Colonic Mucosa in Experimental Colitis of Rats

In order to investigate the protective effects of intestinal trefoil factor (ITF) on colonic mucosa in experimental colitis of rats, ITF was detected by RT-PCR and immunohistochemistry at different time points. Three days after colitis induction, rats were treated with either 0.9 % saline solution or rhITF. Pathological changes and the expression of iNOS mRNA, NO, MDA and SOD were measured respectively. It was found that ITF was mainly located in goblet cells, significantly higher in model group than in normal group (P<0.05). rhITF could increase the iNOS mRNA expression and NO contents, and there was statistically significant difference between rhITF group and model group (P<0.05). rhITF also caused an increase of MDA and a decrease of SOD, but there was no significant difference between two groups. These results indicated that ITF has apparent therapeutic effects in ulcerative colitis, which may be associated with iNOS and NO.

Statins enhance expression of growth factors and activate the PI3K/Akt-mediated signaling pathway after experimental intracerebral hemorrhage

Previous studies have demonstrated that statins improve neurological outcome and promote neuro-vascular recovery after ICH. This study is designed to examine whether simvastatin and atorvastatin affect levels of growth factors and activate the Akt signaling pathway during the recovery phase after intracerebral hemorrhage (ICH) in rats. Sixty (60) male Wistar rats were subjected to ICH by stereotactic injecttion of 100 μL of autologous blood into the striatum and were treated with or without simvastatin or atorvastatin. Neurological functional outcome was evaluated by behavioral tests (mNSS and corner turn test) at different time points after ICH. Brain extracts were utilized for Enzyme-linked immunosorbent assay (ELISA) analyses to measure vascular endothelial growth factor (VEGF);brain-derived neurotrophin factor (BDNF) expression, and nerve growth factor (NGF). Western blot was used to measure the changes in the Akt-mediated signaling pathway. Both the simvastatin and atorvastatin-treated animals had significant neurological improvement at 2 weeks post-ICH. Simvastatin and atorvastatin treatment increased the expression of BDNF, VEGF and NGF in both low- and high-dose groups at 7 days after ICH (p < 0.05). Phosphorylation of Akt, glycogen synthase kinase-3β (GSK-3β), and cAMP response element-binding proteins (CREB) were also increased at 7 days after statin treatment. These results suggest that the therapeutic effects of statins after experimental ICH may be mediated by the transient induction of BDNF, VEGF and NGF expression and the activation of the Akt-mediated signaling pathway.

Tumor necrosis factor-alpha in experimental autoimmune neuritis

Tumor necrosis factor-α （TNF-α） plays a key role in the pathogenesis of experimental autoimmune neuritis （EAN） as well as Guillain-Barre syndrome. The proposed pathogenesis of TNF-α associated neuropathies involves immune-mediated attack to blood-nerve barrier, aggravated production of pro-inflammatory cytokines, and the induction of Schwann cells apoptosis. TNF-α may play a regulatory role by increasing production of interleukin-1 in macrophages, attenuating T cell receptor signaling and regulating apoptosis of potentially autoreactive T cells in EAN. The data suggest that antagonizing TNF-α functions or suppressing TNF-α production may be useful in the acute phase of EAN treatment, but further studies are required.

Role of nuclear factor κB in multiple sclerosis and experimental autoimmune encephalomyelitis

The transcription factor nuclear factor κB（NF-κB） plays major roles in inflammatory diseases through regulation of inflammation and cell viability.Multiple sclerosis（MS） is a chronic inflammatory demyelinating and neurodegenerative disease of the central nervous system（CNS）.It has been shown that NF-κB is activated in multiple cell types in the CNS of MS patients,including T cells,microglia/macrophages,astrocytes,oligodendrocytes,and neurons.Interestingly,data from animal model studies,particularly studies of experimental autoimmune encephalomyelitis,have suggested that NF-κB activation in these individual cell types has distinct effects on the development of MS.In this review,we will cover the current literature on NF-κB and the evidence for its role in the development of MS and its animal model experimental autoimmune encephalomyelitis.

Immunohistochemical study on expression of epidermal growth factor receptor at hepatocyte nuclei in experimental rat liver cirrhosis

ＩｍｍｕｎｏｈｉｓｔｏｃｈｅｍｉｃａｌｓｔｕｄｙｏｎｅｘｐｒｅｓｓｉｏｎｏｆｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒｒｅｃｅｐｔｏｒａｔｈｅｐａｔｏｃｙｔｅｎｕｃｌｅｉｉｎｅｘｐｅｒｉｍｅｎｔａｌｒａｔｌｉｖｅｒｃｉｒｈｏｓｉｓＹＡＮＪｉｎＰｉｎ...

Heat transfer and friction factor of Therminol liquid phase heat transfer fluid in a ribbed tube

Experiments and simulations on flow and heat transfer behavior of Therminol-55 liquid phase heat transfer fluid have been conducted in a ribbed tube with the outer diameter and inner diameter 25.0 and 20.0 mm,pitch and rib height of 4.5 and 1.0 mm.respectively.Experimental results show that the heat transfer and thermal performance of Therminol-55 liquid phase heat transfer fluid in the ribbed tube are considerably improved compared to those of the smooth tube.The Nusselt number increase with the increase of Reynolds number.The increase in heat transfer rate of the ribbed tube has a mean value of 2.24 times.Also,the pressure drop results reveal that the average friction factor of the ribbed tube is in a range of 2.4 and 2.8 times over the smooth tube.Numerical simulations of three-dimensional flow behavior of Therminol-55 liquid phase heat transfer fluid are carried out using three different turbulence models in the ribbed tube.The numerical results show that the heat transfer of ribbed tube is improved because vortices are generated behind ribs,which produce some disruptions to fluid flow and enhance heat transfer compared with smooth tube.The numerical results prove that the ribbed tube can improve heat transfer and fluid flow performances of Therminol liquid phase heat transfer fluid.

Complement factors C1q, C3 and C5b-9 in the posterior sclera of guinea pigs with negative lens-defocused myopia

· AIM: To investigate the expression of complement factors in the posterior scleral fibroblasts of guinea pigs with negative lens-defocused myopia.· METHODS: Eighteen guinea pigs were assigned randomly to two groups: the negative lens-defocused group(NLD group, n =9) and the normal control without treatment group(NC group, n =9). The effect of myopic induction was compared in three subgroups: eyes treated with a-10.00 D negative lens in the NLD group(NL group), eyes treated with a plano(0 D) lens in the NLD group(PL group), and untreated right eyes in the NC group(NC group). The following analyses were conducted at four weeks: examination of the refractive error via retinoscopy, assessment of complement C5b-9expression in the posterior scleral fibroblasts using immunohistochemistry, and measurements of complement C1 q and C3 protein levels in the posterior sclera by Western blot.·RESULTS: After an induction period of four weeks, a significant myopic shift was detected in the eyes of the NL group, relative to that of the PL and NC groups(P 【0.05). Data analysis showed a significant increase in the percentage of C5b-9 immunopositive fibroblasts in the posterior sclera of the NL group eyes, compared to the PL group(q =11.50, P 【0.001). Significantly higher levels of C1q(q =4.94, P =0.01) and C3(q =4.07, P =0.03)protein were detected in the posterior sclera of NL group eyes, compared to the PL group. There were no significant difference between the PL and NC groups for C5b-9(q =2.44, P =0.10), C1q(q =1.55, P =0.53) and C3(q =0.98, P =0.77) in the posterior sclera.·CONCLUSION: The data from present study provide evidence of the up-regulation of C5b-9, C1 q and C3 in the posterior scleral fibroblasts in a NLD myopic animal model. The results suggest that the complement system may be involved in the development of myopia.

Theoretical Analysis and Experimental Verification of Crack Initiation Characteristics of Compression-Shear Plane Crack with Hydraulic Pressure

In this paper, the crack initiation characteristics of compression-shear plane crack with hydraulic pressure were studied by using theoretical analysis and experimental verification methods. The formula derivation process of stress intensity factor of crack tip and open-type crack initiation angle and initiation strength was expounded in detail. Cement mortar specimens prefabricated with open-type crack were made for biaxial compression test. The results show that the mode I stress intensity factor is inversely proportional to the dip angle of pre-exciting crack, water pressure and crack width. The fracture toughness is most easily achieved when the dip angle of pre-exciting crack is 60°. The mode II stress intensity factor is symmetrically distributed with the dip angle and independent of the water pressure and crack width. For open-type crack, the crack initiation angle decreases with the increase of the dip angle of pre-exciting crack, water pressure and crack width;the crack initiation strength is inversely proportional to the water pressure and proportional to the lateral pressure. The research results can provide ideas for the study of crack initiation under the coupling of ground stress and osmotic pressure in tunnel engineering.

N6-甲基腺苷相关调节因子与骨关节炎:生物信息学和实验验证分析

背景:越来越多证据表明N6-甲基腺苷(N6-methyladenosine,m6A)调节因子与骨关节炎密切相关,被认为是防治骨关节炎新方向,但具体作用机制不明。目的:通过对骨关节炎基因芯片数据集进行生物信息学分析,探讨m6A对骨关节炎的作用,解析骨关节炎发病机制。方法:首先利用R软件提取GEO数据库中GSE1919数据集中骨关节炎相关m6A调节因子及其表达量,进而对提取结果行基因差异分析及GO、KEGG富集分析;接着对PPI网络拓扑学分析结果和机器学习结果取交集得到m6A关键调节因子,并通过体外细胞实验验证。结果与结论:①提取得到16个骨关节炎相关m6A调节因子表达量,通过差异分析获得ZC3H13、YTHDC1、YTHDF3、HNRNPC等11个m6A差异调节因子;②GO富集分析显示,骨关节炎相关m6A差异调节因子在生物过程中主要于mRNA转运、RNA分解代谢、胰岛素样生长因子受体信号通路调控等发挥作用;③KEGG富集分析显示,差异调节因子主要参与p53、白细胞介素17和AMPK信号通路;④综合PPI网络拓扑学分析和机器学习结果获得m6A关键调节因子——YTHDC1;⑤体外细胞实验结果表明,m6A关键调节因子——YTHDC1在对照组与骨关节炎组中表达存在显著差异(P<0.05);⑥结果显示,YTHDC1与骨关节炎发生发展密切相关,有望成为m6A治疗骨关节炎的分子靶点。

Methodological factors affecting gas and methane production during in vitro rumen fermentation evaluated by meta-analysis approach

Effects of some methodological factors on in vitro measures of gas production（GP, mL/g DM）, CH4production（mL/g DM） and proportion（% CH4 on total GP） were investigated by meta-analysis. These factors were considered：pressure in the GP equipment（0 = constant; 1 = increasing）, incubation time（0 = 24; 1 = ≥ 48 h）, time of rumen fluid collection（0 = before feeding; 1 = after feeding of donor animals）, donor species of rumen fluid（0 = sheep; 1 =bovine）, presence of N in the buffer solution（0 = presence; 1 = absence）, and ratio between amount of buffered rumen fluid and feed sample（BRF/FS; 0 = ≤ 130 mL/g DM; 1 = 130–140 mL/g DM; 2 = ≥ 140 mL/g DM）. The NDF content of feed sample incubated（NDF） was considered as a continuous variable. From an initial database of 105 papers, 58 were discarded because one of the above-mentioned factors was not stated. After discarding 17 papers,the final dataset comprised 30 papers（339 observations）. A preliminary mixed model analysis was carried out on experimental data considering the study as random factor. Variables adjusted for study effect were analyzed using a backward stepwise analysis including the above-mentioned variables. The analysis showed that the extension of incubation time and reduction of NDF increased GP and CH4 values. Values of GP and CH4 also increased when rumen fluid was collected after feeding compared to before feeding（＋26.4 and ＋9.0 mL/g DM, for GP and CH4）,from bovine compared to sheep（＋32.8 and ＋5.2 mL/g DM, for GP and CH4）, and when the buffer solution did not contain N（＋24.7 and ＋6.7 mL/g DM for GP and CH4）. The increase of BRF/FS ratio enhanced GP and CH4production（＋7.7 and ＋3.3 mL/g DM per each class of increase, respectively）. In vitro techniques for measuring GP and CH4 production are mostly used as screening methods, thus a full standardization of such techniques is not feasible. However, a greater harmonization of analytical procedures（i.e., a reduction in the number of available protocols） would be useful to facilitate comparison between results of different experiments.

采动力学与岩层控制关键理论及工程应用

研究岩体采动力学响应和岩层控制技术对促进煤炭安全高效开采、保障能源稳定供给具有重要意义,是实现煤炭资源科学开采的理论基础。矿山岩体灾害(围岩变形、冲击地压等)频发,其形成-演化-发生全过程与采动力演化分布、岩层运动、开采扰动和能量演化密切相关。基于实用矿山压力控制理论,提出并阐述了采场岩层控制进展与控制准则,建立了定量分析的力学模型和设计方法,发展了针对性的岩体灾害控制技术,并创新研制了配套试验研究装备。采动力学与岩层控制理论将岩层控制分为采场岩层控制和巷道围岩控制;提出控制或利用采动岩层运动改变致灾条件,给出“给定变形”和“限定变形”准则;调控“3S”因素准则(围岩应力环境、围岩结构属性、围岩支护结构)改变围岩自稳能力。以岩体灾害控制为目标,提出了以“应力主控”为核心的释能主控技术;建立了岩体灾害控制大小原理和弱面判据(安全系数K、冲击危险性系数U);研发了采场矿压机械模拟试验系统、采动力试验系统和蠕变及动力扰动冲击加载试验系统,实现了实验室尺度还原采动力作用下岩体变形-破裂-运动过程,为研究采动力作用下岩体力学响应提供了试验装备;分别从采场岩层控制、地质软岩巷道控制、工程软岩巷道控制及冲击地压控制4个方向进行了工程案例研究,相关研究成果在工程应用中得到了验证。

海参皂苷调节SIRT1/NF-κB信号通路对糖尿病大鼠血管炎症的影响

目的:探讨海参皂苷(SSC)对糖尿病大鼠血管炎症的影响及潜在机制。方法:SD大鼠采用高糖高脂饮食和链脲佐菌素(STZ)腹腔注射构建糖尿病模型。实验分为对照(Control)组、模型(model)组、二甲双胍组(600 mg/kg)、SSC组(30 mg/kg)和SSC+沉默信息调节因子1(SIRT1)抑制剂EX-527组(SSC 30 mg/kg+EX-5275 mg/kg)。给予相应的药物干预4周后,检测各组大鼠空腹血糖(FBG)和空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR);酶联免疫吸附法(ELISA)检测血清血管细胞黏附分子-1(VCAM-1)、单核细胞趋化因子-1(MCP-1)、肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)水平;苏木精-伊红(HE)染色观察主动脉病理学变化;蛋白免疫印迹法(Western Blot)检测主动脉SIRT1/核因子-κB(NF-κB)通路相关蛋白的表达。结果:与Control组比较,model组大鼠FBG、FINS、HOMA-IR、血清VCAM-1、MCP-1、TNF-α、IL-1β水平、胞核NF-κB p65蛋白水平升高(P<0.05),体质量、主动脉SIRT1、核因子κB抑制蛋白α(IκBα)蛋白水平降低(P<0.05);与model组比较,SSC组和二甲双胍组大鼠FBG、FINS、HOMA-IR、血清VCAM-1、MCP-1、TNF-α、IL-1β水平、胞核NF-κB p65的蛋白水平降低(P<0.05),体质量、主动脉SIRT1、IκBα蛋白水平升高(P<0.05);与SSC组比较,SSC+EX-527组大鼠FBG、FINS、HOMA-IR、血清VCAM-1、MCP-1、TNF-α、IL-1β水平、胞核NF-κB p65蛋白水平升高(P<0.05),体质量、主动脉SIRT1、IκBα蛋白水平降低(P<0.05)。结论:SSC可减轻糖尿病大鼠血管炎症,其作用机制可能与上调SIRT1表达,抑制NF-κB的活化有关。

Effect of Triptolide on Expression of Receptor Activator of Nuclear Factor-κB Ligand in Rat Adjuvant Induced Arthritis

The effect of triptolide （TP） on the expression of receptor activator of nuclear factor-κB ligand （RANKL） and osteoprotegerin （OPG） was explored in rat adjuvant induced arthritis （AA）. AA was induced in Wistar rats. Arthritis rats were treated with TP and methotrexate （MTX） at the onset （day 9） of arthritis. On the peak of arthritis （day 24）, the expression of RANKL and OPG protein in the joints and RANKL mRNA in peripheral blood mononuclear cells （PBMC） was detected. TNF-α and IL-1β levels in peripheral blood were determined. Bone erosion scores were also evaluated. The results showed that bone erosion scores in TP and MTX groups were lower than in AA group （.P〈0.01） ; The expression levels of RANKL in the synovium （P〈0.01） and bone （P〈0.05）, and OPG level in synovium （P〈0.05） were lower in TP group than in AA group （P〈0.05）. In TP group, the expression levels of RANKL mRNA and TNF-α, IL-1β in PBMC were lower than in AA group （all P〈0.01）. It was concluded that TP could inhibit rat adjuvant arthritis bone erosion by suppressing the expression of RANKL.

“探究环境因素对黑藻光合作用强度的影响”实验设计

对高中生物学实验“探究环境因素对光合作用强度的影响”的实验材料和方法进行拓展。以水生植物黑藻为实验材料,利用自制的简易光照箱为实验装置,同时探究两个环境因素(光照强度、CO_(2)浓度)对黑藻光合作用强度的影响,定量分析实验结果,建立变量间的数量变化关系。该实验设计可缩短实验时间,增强实验效果,提高实验效率。

TUMOR NECROSIS FACTOR－α ALTERS PROTEINMETABOLISM AND CELL－CYCLE KINETICSIN MALIGNANT TUMOR

The effects of tumor necrosis factor-α(TNF) on protein metabolism and cell-cycle kinetics were investigated in malignant tumor. Sprague-Dawley rats, subcutaneously inoculated with Walker 256 carcinosarcoma,were injected intraperitoneally with recombinant human TNF at a dose of 4-75×106 U/kg for 3 consecutive days.Tumor protein metabolism and cell-cycle kinetics were analyzed. The results showed a significant decrease in tumor volume and weight in comparison with control.TNF resulted in significant decrease in tumor Protein fractional synthesis rate, Protein synthesis and fractional growth rate, but no change of tumor protein fractional degradation rate. TNF also resulted in remarkable decline in labelling index and GI phase increase of tumor cells, 6 hours after bromodeoxyuridine injection, by cytometry. The results indicated that TNF inhibits tumor growth as a result of decreases in tumor cell DNA and protein syntheses.

白云岩的成因、储集空间及实验技术研究新进展

全球广布的白云岩中蕴含着丰富的油气资源,但是白云岩的成因机理和储集空间保持机制还存在诸多疑问。分析研究了近年来白云岩的成因机理、储集空间控制因素与新型实验技术等方面研究取得的重大进展。(1)通过溶解-再沉淀方法成功合成出低温有序白云石,白云岩的形成受热力学和动力学屏障共同制约。(2)研究建立、拓展和完善了多种白云石化模型。新建了有机物(微生物)诱导模型,完善了无机物催化模型,修订了混合水白云石化模型,拓展了蒸发泵和渗透回流白云石化模型,细化了埋藏白云石化类型。(3)原始沉积环境和后期成岩改造对白云岩储层演化具有重要控制作用。适度的白云石化作用及次生溶蚀作用增加白云岩储层的孔隙空间,过度的胶结作用、大量热液矿物沉淀及轻-中度重结晶作用减少白云岩储层的孔隙空间。(4)发展了微区元素分析、Mg-Ca同位素、激光原位U-Pb同位素、团簇同位素、激光共聚焦-CT扫描成像解析及核磁共振-频谱激发极化实验新技术。研发了白云岩储层正演模拟技术、多尺度定量表征白云岩孔隙的三维结构和流动性技术,为白云石成因机理、储集空间研究提供重要的技术支持。

Nogo receptor expression in microglia/macrophages during experimental autoimmune encephalomyelitis progression

Myelin-associated inhibitory factors within the central nervous system（CNS） are considered to be one of the main obstacles for axonal regeneration following disease or injury. The nogo receptor 1（NgR1） has been well documented to play a key role in limiting axonal regrowth in the injured and diseased mammalian CNS. However, the role of nogo receptor in immune cell activation during CNS inflammation is yet to be mechanistically elucidated. Microglia/macrophages are immune cells that are regarded as pathogenic contributors to inflammatory demyelinating lesions in multiple sclerosis（MS）. In this study, the animal model of MS, experimental autoimmune encephalomyelitis（EAE） was induced in ngr1^＋/＋ and ngr1^–/– female mice following injection with the myelin oligodendrocyte glycoprotein（MOG_（35–55）） peptide. A fatemap analysis of microglia/macrophages was performed throughout spinal cord sections of EAE-induced mice at clinical scores of 0, 1, 2 and 3, respectively（increasing locomotor disability） from both genotypes, using the CD11 b and Iba1 cell markers. Western immunoblotting using lysates from isolated spinal cord microglia/macrophages, along with immunohistochemistry and flow cytometric analysis, was performed to demonstrate the expression of nogo receptor and its two homologs during EAE progression. Myelin protein engulfment during EAE progression in ngr1^＋/＋ and ngr1^–/– mice was demonstrated by western immunblotting of lysates from isolated spinal cord microglia/macrophages, detecting levels of Nogo-A and MOG. The numbers of M1 and M2 microglia/macrophage phenotypes present in the spinal cords of EAE-induced ngr1^＋/＋ and ngr1^–/– mice, were assessed by flow cytometric analysis using CD38 and Erg-2 markers. A significant difference in microglia/macrophage numbers between ngr1^＋/＋ and ngr1^–/– mice was identified during the progression of the clinical symptoms of EAE, in the white versus gray matter regions of the spinal cord. This difference was unrelated to the expression of Ng R on these macrophage/microglial cells. We have identified that as EAE progresses, the phagocytic activity of microglia/macrophages with myelin debris, in ngr1^–/– mice, was enhanced. Moreover, we show a modulation from a predominant M1-pathogenic to the M2-neurotrophic cell phenotype in the ngr1^–/– mice during EAE progression. These findings suggest that CNS-specific macrophages and microglia of ngr1^–/– mice may exhibit an enhanced capacity to clear inhibitory molecules that are sequestered in inflammatory lesions.

颗粒表面Zeta电位测量实验项目改革

在传统的Zeta电位测量实验项目的基础上,通过引入多种电解质、分散剂和沉降实验,对实验内容进行扩充,使学生能全面探索Zeta电位的影响因素,深化理论知识。同时,结合教学视频,使学习过程更加直观生动,显著提升了学生的学习效果和实验操作能力。这些改革不仅激发了学生的学习热情,还锻炼了他们的分析问题和解决问题的能力,取得了显著的教学成果。

Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF,TNFα,and caspase 3 levels in an experimental diabetes model

AIM: To evaluate the influence of an intravitreal injection of bevacizumab and fasudil on the retinal vascular endothelial growth factor(VEGF),tumor necrosis factor alpha(TNFα),and caspase 3 levels in a diabetic rabbit model. · METHODS: The study included 6 healthy rabbits(Group 1),6 rabbits with experimentally induced diabetes mellitus(DM)(Group 2),7 rabbits with experimentally induced DM to which intravitreal bevacizumab was administered(Group 3),and 7 rabbits with experimentally induced DM to which intravitreal fasudil was administered(Group 4). An intravitreal injection of 1.25mg/50μL bevacizumab in the right eye of rabbits in Group 3 and an intravitreal injection of 0.0064mg/50μL fasudil in the right eye of rabbits in Group 4 were administered on day 21 after the induction of DM. The studied eyes of the rabbits were enucleated three days after the intravitreal injection. The TNFα,VEGF,and caspase 3 levels were determined using the ELISA method. · RESULTS: There was a statistically significant difference in the VEGF and caspase 3 levels between groups(P =0.005 and P =0.013,respectively),but the TNF α level did not differ significantly between groups(P = 0.792). It was found that VEGF levels were significantly lower in Group 1 and Group 3 than in Group 2 using the Mann-Whitney U test with the Bonferroni correction(P = 0.004 for both comparison). There was no statistically significant difference between other groups with regard to VEGF levels(the P value ranged between 0.015 and 0.886). Although the P values of the caspase 3 levels were 0.015 for Group 1 and Group 4,0.038 for Group 2and Group 3,and 0.018 for Group 3 and Group 4,these P values remained above the threshold P value of 0.0083,which was the statistically significant level for post hoc tests. ·CONCLUSION: An intravitreal injection of bevacizumab decreased both the VEGF level,which plays a role in angiogenesis,and the caspase 3 level,which plays a role in apoptosis. Although not as effective as bevacizumab,fasudil had a beneficial effect on the VEGF levels but significantly increased the caspase 3 levels.

Reflection on the Construction of Tropical and South Subtropical Agricultural Experimental Station CATAS( Jiangmen)

This paper introduces the origin and progress of Tropical and South Subtropical Agricultural Comprehensive Experimental Station( hereinafter referred to as experimental station) CATAS( Jiangmen),formulates the thought for construction of experimental station,analyzes the restrictive factors and puts forward corresponding countermeasures in order to provide reference for the decision- making and implementation of constructing the experimental station.