Histological Analysis and Morphometric Changes after Autologous Fat Grafting in Large Volumes on Pigs

Nowadays, fat transfer is an accepted technique that has been popularized in clinical practice, during which large volumes of fat are strategically relocated to increase and improve the contour of the body. In the present study, we investigated the histological and morphometric changes after large volumes of fat were grafted within the subcutaneous cellular tissue. An experimental study was designed using four 3-month-old male pigs. One hundred cc of decanted fat tissue was transferred into the right leg. Volume augmentation achieved by auto lipo-injecting within the subcutaneous cellular tissue persisted up to two months. Although there is a reduction in cell number and size, an increased amount of extracellular matrix and the presence of oily cysts and microcalcifications were observed. Conclusion: The volume augmentation, which is achieved by autologous fat transfer in large quantities in subcutaneous cellular tissue, can remain for months.

Fat injection to correct contour deformities of the reconstructed breast:a single surgeon experience

Aim:Autologous fat grafting has gained acceptance as a technique to improve aesthetic outcomes in breast reconstruction.The purpose of this study was to share our clinical experience using autologous fat injection to correct contour deformities during breast reconstruction.Methods:A single surgeon,prospectively maintained database of patients who underwent autologous fat injection during breast reconstruction from January 2008 to November 2013 at McGill University Health Center was reviewed.Patient characteristics,breast history,type of breast reconstruction,volume of fat injected,and complications were analyzed.Results:One hundred and twenty-four patients benefited from autologous fat injection from January 2008 to November 2013,for a total of 187 treated breasts.The patients were on average 49.3 years old(±8.9 years).Fat was harvested from the medial thighs(20.5%),flanks(39.1%),medial thighs and flanks(2.9%),trochanters(13.3%),medial knees(2.7%),and abdomen(21.9%).An average of 49.25 mL of fat was injected into each reconstructed breast.A total of 187 breasts in 124 patients were lipo-infiltrated during the second stage of breast reconstruction.Thirteen breasts(in 12 separate patients)were injected several years after having undergone lumpectomy and radiotherapy.Of the 187 treated breasts,118 were reconstructed with expanders to implants,45 with deep inferior epigastric perforator flaps,9 with latissimus dorsi flaps with implants,4 with transverse rectus abdominis myocutaneous flaps,and 13 had previously undergone lumpectomy and radiotherapy.Six complications were noted in the entire series,for a rate of 3.2%.All were in previously radiated breasts.Average follow-up time was 12 months(range:2-36 months).Conclusion:Fat injection continues to grow in popularity as an adjunct to breast reconstruction.Our experience demonstrates a low complication rate as compared to most surgical interventions of the breast and further supports its safety in breast reconstruction.However,caution should be used when treating previously radiated breasts.