This study aims to investigate the effects of EGCG on the lipid deposition and liver anti-oxidative capacity of broilers under heat stress.One hundred and ninety-two 2-week-old broilers were divided into four groups w...This study aims to investigate the effects of EGCG on the lipid deposition and liver anti-oxidative capacity of broilers under heat stress.One hundred and ninety-two 2-week-old broilers were divided into four groups with 6 replicates per group and 8 chickens per replicate:one thermoneutral control group(28℃,TN group),which was fed the basal diet,and three cyclic high-temperature groups(35℃from 7:00 to 19:00 h;28℃from 19:00 h to 7:00 h,heat stress(HS)group),which were fed the basal diet added with EGCG at doses of 0(HS0 group),300(HS300 group),and 600 mg/kg(HS600 group),respectively.The liver metabolism and lipid deposition indices were performed at 35 d of age.The results showed that heat stress decreased the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),and the Nrf2 mRNA expression in liver,and increase significantly the levels of malondialdehyde(MDA)and the expression of LITAF,NF-KB,FAS,SREBP1 mRNA and the lipid deposition compared with TN group.EGCG(HS300 and HS600 group)increased the activities of SOD and GSH-Px and catalase(CAT),increased the Nrf2 mRNA expression,decreased the MDA contents,and reduced the lipid deposition and expression of LITAF,NF-KB,FAS and SREBP1 mRNA.In conclusion,the results of this study show that EGCG can improve liver antioxidative capacity to alleviate oxidative damage caused by heat stress.展开更多
Objective To investigate the prevalence and possible factors influencing metabolic syndrome in people from Guizhou Province and to explore the predictive value of the fat-to-muscle ratio in diagnosing metabolic syndro...Objective To investigate the prevalence and possible factors influencing metabolic syndrome in people from Guizhou Province and to explore the predictive value of the fat-to-muscle ratio in diagnosing metabolic syndrome. Methods A multistage stratified sampling method was used in this cross-sectional study of 20-80 years old Han and Bouyei populations from Guizhou Province, southwestern China, from October-December 2012. The study included 4,553 cases of metabolic syndrome, that was defined according to 2005 International Diabetes Federation criteria. The receiver operating characteristic curve was used for determining the sensitivity, specificity, and predictive ability of the fat-to-muscle ratio for the diagnosis of metabolic syndrome. Results The age-standardized prevalence of metabolic syndrome was 11.38%(men: 9.76%; women: 12.72%) for Han and 4.78%(men: 4.43%; women: 5.30%) for Bouyei populations. In Guizhou Province, the cut-off value for the men fat-to-muscle ratio was 0.34, the area under the curve was 0.95, and the sensitivity and specificity were 0.94 and 0.85, respectively. The cut-off value for the women fat-to-muscle ratio was 0.55, the area under the curve was 0.91, and the sensitivity and specificity were 0.93 and 0.79, respectively. Conclusion The fat-to-muscle ratio is highly predictive of metabolic syndrome in Guizhou Province, and a useful reference indicator.展开更多
The study was carried out to investigate the effects of maternal dietary energy restriction on growth performance, serum indices and fat deposition of offspring. A total of 400 female Arbor Acres (AA) broiler breede...The study was carried out to investigate the effects of maternal dietary energy restriction on growth performance, serum indices and fat deposition of offspring. A total of 400 female Arbor Acres (AA) broiler breeders were studied. These female birds involved three experimental treatments and a control group with normal dietary energy diets (ND, 11.7 MJ of ME. kgt during the laying). In treatments 2, 3 and 4, the energies of diets were 20%, 30% and 50% (LD20, LD30 and LD50) lower than those of the control, respectively. The study commenced at the beginning of the laying period when the total egg production reached 5% of the flock. All the broiler offspring were fed the same diets. The results showed that in low energy diets, offspring showed decreased 1-day-old weight, but 49-day-old weight was higher in LD20 diet (P〈0.05). For offspring during days 1-49, the average daily gain (ADG) in LD20 group and the feed conversion ratio in LD50 group were improved as compared with those of the control (P〈0.05). Compared with the control, abdominal fat percentage increased in 49-day-old offspring from LD30 diet (P〈0.05); the fat content of breast muscle in offspring increased in broilers fed low energy diets (P〈0.05). In 28-day-old offspring from breeders given LD20 and LD50 diets, liver fat percentages were higher compared with ND (P〈0.05). The subcutaneous fat thickness in 28-day-old offspring from LD50 group and 49-day-old offspring from LD30 group was higher (P〈0.05). On day 49, the serum cholesterol (CHO) of offspring from breeders fed LD20 diet and serum high-density lipoprotein (HDL) of offspring from breeders fed LD50 diet reduced compared with those of the control (P〈0.05). In addition, a higher triiodothyronine (T3) content in serum was found in offspring from broiler breeders given LD20 and LD30 diets (P〈0.05). Serum thyroxine (T4) in offspring significantly decreased with the decrease of diet energy (P〈0.05). In conclusion, to a certain extent, dietary energy restriction in breeders could improve growth performance and promote lipid metabolism of offspring.展开更多
The term lipidome is mentioned to the total amount of the lipids inside the biological cells.The lipid enters the human gastrointestinal tract through external source and internal source.The absorption pathway of lipi...The term lipidome is mentioned to the total amount of the lipids inside the biological cells.The lipid enters the human gastrointestinal tract through external source and internal source.The absorption pathway of lipids in the gastrointestinal tract has many ways;the 1st way,the lipid molecules are digested in the lumen before go through the enterocytes,digested products are re-esterified into complex lipid molecules.The 2nd way,the intracellular lipids are accumulated into lipoproteins(chylomicrons)which transport lipids throughout the whole body.The lipids are re-synthesis again inside the human body where the gastrointestinal lipids are:(1)Transferred into the endoplasmic reticulum;(2)Collected as lipoproteins such as chylomicrons;or(3)Stored as lipid droplets in the cytosol.The lipids play an important role in many stages of the viral replication cycle.The specific lipid change occurs during viral infection in advanced viral replication cycle.There are 47 lipids within 11 lipid classes were significantly disturbed after viral infection.The virus connects with blood-borne lipoproteins and apolipoprotein E to change viral infectivity.The viral interest is cholesterol-and lipid raft-dependent molecules.In conclusion,lipidome is important in gastrointestinal fat absorption and coronavirus disease 2019(COVID-19)infection so lipidome is basic in gut metabolism and in COVID-19 infection success.展开更多
Reproduction, fat metabolism, and longevity are inter- twined regulatory axes; recent studies in C. elegans have provided evidence that these processes are directly coupled. However, the mechanisms by which they are c...Reproduction, fat metabolism, and longevity are inter- twined regulatory axes; recent studies in C. elegans have provided evidence that these processes are directly coupled. However, the mechanisms by which they are coupled and the reproductive signals modu- lating fat metabolism and lifaspan are poorly under- stood. Here, we find that an oogenesis-enriched gene, c30PI2.4, is specifically expressed and located in germ cells and early embryos; when the gene is knocked out, oogenesis is disrupted and brood size is decreased. In addition to the reproductive phenotype, we find that the loss of c30f12.4 alters fat metabolism, resulting in decreased fat storage and smaller lipid droplets. Mean- while, c30f12.4 mutant worms display a shortened lifaspan. Our results highlight an important role for c30f12.4 in regulating reproduction, fat homeostasis, and aging in C. elegans, which helps us to better understand the relationship between these processes.展开更多
文摘This study aims to investigate the effects of EGCG on the lipid deposition and liver anti-oxidative capacity of broilers under heat stress.One hundred and ninety-two 2-week-old broilers were divided into four groups with 6 replicates per group and 8 chickens per replicate:one thermoneutral control group(28℃,TN group),which was fed the basal diet,and three cyclic high-temperature groups(35℃from 7:00 to 19:00 h;28℃from 19:00 h to 7:00 h,heat stress(HS)group),which were fed the basal diet added with EGCG at doses of 0(HS0 group),300(HS300 group),and 600 mg/kg(HS600 group),respectively.The liver metabolism and lipid deposition indices were performed at 35 d of age.The results showed that heat stress decreased the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),and the Nrf2 mRNA expression in liver,and increase significantly the levels of malondialdehyde(MDA)and the expression of LITAF,NF-KB,FAS,SREBP1 mRNA and the lipid deposition compared with TN group.EGCG(HS300 and HS600 group)increased the activities of SOD and GSH-Px and catalase(CAT),increased the Nrf2 mRNA expression,decreased the MDA contents,and reduced the lipid deposition and expression of LITAF,NF-KB,FAS and SREBP1 mRNA.In conclusion,the results of this study show that EGCG can improve liver antioxidative capacity to alleviate oxidative damage caused by heat stress.
基金the National Science and Technology Pillar Program during the Twelfth Five-Year Plan Period sponsored by the Ministry of Science and Technology of China[grant number 2012BAI37B02]the National Natural Science Foundation of China[grant number 81673184]
文摘Objective To investigate the prevalence and possible factors influencing metabolic syndrome in people from Guizhou Province and to explore the predictive value of the fat-to-muscle ratio in diagnosing metabolic syndrome. Methods A multistage stratified sampling method was used in this cross-sectional study of 20-80 years old Han and Bouyei populations from Guizhou Province, southwestern China, from October-December 2012. The study included 4,553 cases of metabolic syndrome, that was defined according to 2005 International Diabetes Federation criteria. The receiver operating characteristic curve was used for determining the sensitivity, specificity, and predictive ability of the fat-to-muscle ratio for the diagnosis of metabolic syndrome. Results The age-standardized prevalence of metabolic syndrome was 11.38%(men: 9.76%; women: 12.72%) for Han and 4.78%(men: 4.43%; women: 5.30%) for Bouyei populations. In Guizhou Province, the cut-off value for the men fat-to-muscle ratio was 0.34, the area under the curve was 0.95, and the sensitivity and specificity were 0.94 and 0.85, respectively. The cut-off value for the women fat-to-muscle ratio was 0.55, the area under the curve was 0.91, and the sensitivity and specificity were 0.93 and 0.79, respectively. Conclusion The fat-to-muscle ratio is highly predictive of metabolic syndrome in Guizhou Province, and a useful reference indicator.
基金Supported by the Education Department Research Program of Heilongjiang Province(12531036)Doctor Science Foundation of Northeast Agricultural University(2009RC28)
文摘The study was carried out to investigate the effects of maternal dietary energy restriction on growth performance, serum indices and fat deposition of offspring. A total of 400 female Arbor Acres (AA) broiler breeders were studied. These female birds involved three experimental treatments and a control group with normal dietary energy diets (ND, 11.7 MJ of ME. kgt during the laying). In treatments 2, 3 and 4, the energies of diets were 20%, 30% and 50% (LD20, LD30 and LD50) lower than those of the control, respectively. The study commenced at the beginning of the laying period when the total egg production reached 5% of the flock. All the broiler offspring were fed the same diets. The results showed that in low energy diets, offspring showed decreased 1-day-old weight, but 49-day-old weight was higher in LD20 diet (P〈0.05). For offspring during days 1-49, the average daily gain (ADG) in LD20 group and the feed conversion ratio in LD50 group were improved as compared with those of the control (P〈0.05). Compared with the control, abdominal fat percentage increased in 49-day-old offspring from LD30 diet (P〈0.05); the fat content of breast muscle in offspring increased in broilers fed low energy diets (P〈0.05). In 28-day-old offspring from breeders given LD20 and LD50 diets, liver fat percentages were higher compared with ND (P〈0.05). The subcutaneous fat thickness in 28-day-old offspring from LD50 group and 49-day-old offspring from LD30 group was higher (P〈0.05). On day 49, the serum cholesterol (CHO) of offspring from breeders fed LD20 diet and serum high-density lipoprotein (HDL) of offspring from breeders fed LD50 diet reduced compared with those of the control (P〈0.05). In addition, a higher triiodothyronine (T3) content in serum was found in offspring from broiler breeders given LD20 and LD30 diets (P〈0.05). Serum thyroxine (T4) in offspring significantly decreased with the decrease of diet energy (P〈0.05). In conclusion, to a certain extent, dietary energy restriction in breeders could improve growth performance and promote lipid metabolism of offspring.
文摘The term lipidome is mentioned to the total amount of the lipids inside the biological cells.The lipid enters the human gastrointestinal tract through external source and internal source.The absorption pathway of lipids in the gastrointestinal tract has many ways;the 1st way,the lipid molecules are digested in the lumen before go through the enterocytes,digested products are re-esterified into complex lipid molecules.The 2nd way,the intracellular lipids are accumulated into lipoproteins(chylomicrons)which transport lipids throughout the whole body.The lipids are re-synthesis again inside the human body where the gastrointestinal lipids are:(1)Transferred into the endoplasmic reticulum;(2)Collected as lipoproteins such as chylomicrons;or(3)Stored as lipid droplets in the cytosol.The lipids play an important role in many stages of the viral replication cycle.The specific lipid change occurs during viral infection in advanced viral replication cycle.There are 47 lipids within 11 lipid classes were significantly disturbed after viral infection.The virus connects with blood-borne lipoproteins and apolipoprotein E to change viral infectivity.The viral interest is cholesterol-and lipid raft-dependent molecules.In conclusion,lipidome is important in gastrointestinal fat absorption and coronavirus disease 2019(COVID-19)infection so lipidome is basic in gut metabolism and in COVID-19 infection success.
文摘Reproduction, fat metabolism, and longevity are inter- twined regulatory axes; recent studies in C. elegans have provided evidence that these processes are directly coupled. However, the mechanisms by which they are coupled and the reproductive signals modu- lating fat metabolism and lifaspan are poorly under- stood. Here, we find that an oogenesis-enriched gene, c30PI2.4, is specifically expressed and located in germ cells and early embryos; when the gene is knocked out, oogenesis is disrupted and brood size is decreased. In addition to the reproductive phenotype, we find that the loss of c30f12.4 alters fat metabolism, resulting in decreased fat storage and smaller lipid droplets. Mean- while, c30f12.4 mutant worms display a shortened lifaspan. Our results highlight an important role for c30f12.4 in regulating reproduction, fat homeostasis, and aging in C. elegans, which helps us to better understand the relationship between these processes.