期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
Leucosceptroid B from glandular trichomes of Leucosceptrum canum reduces fat accumulation in Caenorhabditis elegans through suppressing unsaturated fatty acid biosynthesis 被引量:1
1
作者 LING Yi TENG Lin-Lin +5 位作者 HUA Juan LI De-Sen LUO Shi-Hong LIU Yan-Chun LIU Yan LI Sheng-Hong 《Chinese Journal of Natural Medicines》 SCIE CAS CSCD 2019年第12期892-899,共8页
Obesity that is highly associated with numerous metabolic diseases has become a global health issue nowdays.Plant sesterterpenoids are an important group of natural products with great potential;thus,their bioactiviti... Obesity that is highly associated with numerous metabolic diseases has become a global health issue nowdays.Plant sesterterpenoids are an important group of natural products with great potential;thus,their bioactivities deserve extensive exploration.RNA-seq analysis indicated that leucosceptroid B,a sesterterpenoid previously discovered from the glandular trichomes of Leucosceptrum canum,significantly regulated the expression of 10 genes involved in lipid metabolism in Caenorhabditis elegans.Furthermore,leucosceptroid B was found to reduce fat storage,and downregulate the expression of two stearoyl-CoA desaturase(SCD)genes fat-6 and fat-7,and a fatty acid elongase gene elo-2 in wild-type C.elegans.In addition,leucosceptroid B significantly decreased fat accumulation in both fat-6 and fat-7 mutant worms but did not affect the fat storage of fat-6;fat-7 double mutant.These findings indicated that leucosceptroid B reduced fat storage depending on the downregulated expression of fat-6,fat-7 and elo-2 and thereby inhibiting the biosynthesis of the corresponding unsaturated fatty acid.These findings provide new insights into the development and utilization of plant sesterterpenoids as potential antilipemic agents. 展开更多
关键词 Sesterterpenoid Leucosceptroid B Leucosceptrum canum Caenorhabditis elegans Fat accumulation fatty acid biosynthesis Antilipemic agent
原文传递
Chain Elongation Using Native Soil Inocula:Exceptional n-Caproate Biosynthesis Performance and Microbial Mechanisms
2
作者 Lin Deng Yang Lv +5 位作者 Tian Lan Qing-Lian Wu Wei-Tong Ren Hua-Zhe Wang Bing-Jie Ni Wan-Qian Guo 《Engineering》 SCIE EI CAS CSCD 2024年第8期262-272,共11页
This study demonstrates the feasibility and effectiveness of utilizing native soils as a resource for inocula to produce n-caproate through the chain elongation(CE)platform,offering new insights into anaerobic soil pr... This study demonstrates the feasibility and effectiveness of utilizing native soils as a resource for inocula to produce n-caproate through the chain elongation(CE)platform,offering new insights into anaerobic soil processes.The results reveal that all five of the tested soil types exhibit CE activity when supplied with high concentrations of ethanol and acetate,highlighting the suitability of soil as an ideal source for n-caproate production.Compared with anaerobic sludge and pit mud,the native soil CE system exhibited higher selectivity(60.53%),specificity(82.32%),carbon distribution(60.00%),electron transfer efficiency(165.00%),and conductivity(0.59 ms∙cm^(-1)).Kinetic analysis further confirmed the superiority of soil in terms of a shorter lag time and higher yield.A microbial community analysis indicated a positive correlation between the relative abundances of Pseudomonas,Azotobacter,and Clostridium and n-caproate production.Moreover,metagenomics analysis revealed a higher abundance of functional genes in key microbial species,providing direct insights into the pathways involved in n-caproate formation,including in situ CO_(2)utilization,ethanol oxidation,fatty acid biosynthesis(FAB),and reverse beta-oxidation(RBO).The numerous functions in FAB and RBO are primarily associated with Pseudomonas,Clostridium,Rhodococcus,Stenotrophomonas,and Geobacter,suggesting that these genera may play roles that are involved or associated with the CE process.Overall,this innovative inoculation strategy offers an efficient microbial source for n-caproate production,underscoring the importance of considering CE activity in anaerobic soil microbial ecology and holding potential for significant economic and environmental benefits through soil consortia exploration. 展开更多
关键词 SOIL Chain elongation n-caproate Reverse beta-oxidation fatty acid biosynthesis METAGENOMICS
下载PDF
Studies on HUFA Biosynthesis in a Marine Teleost Siganus Canaliculatus
3
作者 Yuan-You Li Liang Zhang Su-Qi Wang Shu-De Xu Chang-bo Hu Yi-Jun Zheng 《Feed & Livestock》 2010年第2期7-7,共1页
Marine fish are incapable or have a deft- cient capacity to synthesize highly unsaturated fatty acids (HUFA) from C18 precursors, and thus fish meal and fish oil (FO) are needed in their formulated diets.
关键词 Marine fish biosynthesis of poly- unsat-urated fatty acids Nutritional regulation Salinityregulation
下载PDF
Modeling and targeting an essential metabolic pathway of Plasmodium falciparum in apicoplast using Petri nets
4
作者 Sakshi Gupta Gajendra Pratap Singh Sunita Kumawat 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2022年第1期91-110,共20页
Petri net(PN) is one of the promising computational and mathematical formalisms used to represent and study the behavior of complex metabolic networks. The various available analysis techniques of PN could be used to ... Petri net(PN) is one of the promising computational and mathematical formalisms used to represent and study the behavior of complex metabolic networks. The various available analysis techniques of PN could be used to validate and analyze the network in different scenarios. Plasmodium falciparum is one of the threatening parasites which causes malaria, a deadly disease affecting a large number of today’s world population. The development of antimalarial drug resistance is an emerging global threat, highlighting the need to discover novel antimalarial targets. The fatty acid biosynthesis of malarial parasite is one of the essential metabolic pathways required for its growth and is present in apicoplast, a non-photosynthetic plastid. The malarial parasite obtains fatty acids by using type two fatty acid synthase(FAS II) enzyme,which is different from type one enzyme used by human host, making it an ideal drug target.This article proposes and studies the PN model of the parasite’s FAS II pathway to analyze the mechanism of potential drug targets in this pathway. The proposed PN model can serve as a base for further findings in the field of antimalarial drug targets to decrease the malaria mortality rate. 展开更多
关键词 Petri net MALARIA Plasmodium falciparum type II fatty acid biosynthesis drug target property analysis
下载PDF
HCV NS5A and NS5B Enhance Expression of Human Ceramide Glucosyltransferase Gene
5
作者 Jia Guo Ran Yan +1 位作者 Guo-dong Xu Cong-yi Zheng 《Virologica Sinica》 CAS CSCD 2012年第1期38-47,共10页
Host genes involved in lipid metabolism are differentially affected during the early stages of hepatitis C virus (HCV) infection. Here we demonstrate that artificial up-regulation of fatty acid biosynthesis has a po... Host genes involved in lipid metabolism are differentially affected during the early stages of hepatitis C virus (HCV) infection. Here we demonstrate that artificial up-regulation of fatty acid biosynthesis has a positive effect on the replication of the HCV full-length replicon when cells were treated with nystatin. Conversely, the HCV RNA replication was decreased when fatty acid biosynthesis was inhibited with 25-hydroxycholesterol and PDMP(D-threo-l-phenyl-2-decanoylamino-3- morpholino-l-propanol). In agreement with these results, the expression level of GlcT-l(ceramide glucosyltransferase), a host glucosyltransferase in the first step of GSL (glycosphingolipid) biosynthesis, was found to be closely associated with the expression and replication of HCV RNA. On the other hand, the viral RNA can also activate GlcT-1 in the early stage of viral RNA transfection in vitro. To identify viral factors that are responsible for GlcT-1 activation, we constructed ten stable Vero cell lines that express individual HCV proteins. Based on the analyses of these cell lines and transient transfection assay of the GlcT-1 promoter regions, we conclude that HCV proteins, especially NS5A and NS5B, have positive effects on the expression of GlcT-1. It is possible that NS5A and NS5B stimulate transcription factor(s) to activate the expression of GlcT-1 by increasing its transcription level 展开更多
关键词 Hepatitis C virus fatty acid biosynthesis Ceramide glucosyltransferase Stable cell lines
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部