Exploration of biodemulsifiers has become a new research aspect.Using waste frying oils（WFOs） as carbon source to synthesize biodemulsifiers has a potential prospect to decrease production cost and to improve the ap...Exploration of biodemulsifiers has become a new research aspect.Using waste frying oils（WFOs） as carbon source to synthesize biodemulsifiers has a potential prospect to decrease production cost and to improve the application of biodemulsifiers in the oilfield.In this study,a demulsifying strain,Alcaligenes sp.S-XJ-1,was investigated to synthesize a biodemulsifier using waste frying oils as carbon source.It was found that the increase of initial pH of culture medium could increase the biodemulsifier yield but decrease the demulsification ratio compared to that using paraffin as carbon source.In addition,a biodemulsifier produced by waste frying oils and paraffin as mixed carbon source had a lower demulsification capability compared with that produced by paraffin or waste frying oil as sole carbon source.Fed-batch fermentation of biodemulsifier using waste frying oils as supplementary carbon source was found to be a suitable method.Mechanism of waste frying oils utilization was studied by using tripalmitin,olein and tristearin as sole carbon sources to synthesize biodemulsifier.The results showed saturated long-chain fatty acid was diffcult for S-XJ-1 to utilize but could effectively enhance the demulsification ability of the produced biodemulsifier.Moreover,FT-IR result showed that the demulsification capability of biodemulsifiers was associated with the content of C=O group and nitrogen element.展开更多
基金supported by the National Natural Science Foundation of China(No.50908166)the China Postdoctoral Science Foundation(No.20100480621)+2 种基金the Program for New Century Excellent Talents in University of ChinaProgram for Young Excellent Talents in Tongji University(No.2010KJ060)the International S&T Cooperation Program of China(No.2009DFA92140)
文摘Exploration of biodemulsifiers has become a new research aspect.Using waste frying oils（WFOs） as carbon source to synthesize biodemulsifiers has a potential prospect to decrease production cost and to improve the application of biodemulsifiers in the oilfield.In this study,a demulsifying strain,Alcaligenes sp.S-XJ-1,was investigated to synthesize a biodemulsifier using waste frying oils as carbon source.It was found that the increase of initial pH of culture medium could increase the biodemulsifier yield but decrease the demulsification ratio compared to that using paraffin as carbon source.In addition,a biodemulsifier produced by waste frying oils and paraffin as mixed carbon source had a lower demulsification capability compared with that produced by paraffin or waste frying oil as sole carbon source.Fed-batch fermentation of biodemulsifier using waste frying oils as supplementary carbon source was found to be a suitable method.Mechanism of waste frying oils utilization was studied by using tripalmitin,olein and tristearin as sole carbon sources to synthesize biodemulsifier.The results showed saturated long-chain fatty acid was diffcult for S-XJ-1 to utilize but could effectively enhance the demulsification ability of the produced biodemulsifier.Moreover,FT-IR result showed that the demulsification capability of biodemulsifiers was associated with the content of C=O group and nitrogen element.
