Lung cancer is the leading cause of cancer-related mortality in the world. Although surgery, chemotherapy and radiotherapy can improve the survival rate l, cancers still affect people's quality of life. Feiji Reci...Lung cancer is the leading cause of cancer-related mortality in the world. Although surgery, chemotherapy and radiotherapy can improve the survival rate l, cancers still affect people's quality of life. Feiji Recipe is an empirical formula and has been used as a treatment for non-small cell lung cancer(NSCLC) for many years. This thesis collects data of the etiology and pathogenesis of Feiji Recipe, clinical research and animal experiments,and provide suggestive evidence of Feiji Recipe for treating NSCLC. During the treatment, the characteristic of holism and personalized treatment of traditional Chinese medicine(TCM)is proved, which also demonstrated that it can relieve patient's clinical syndrome, improve their quality of life and enhance the body immunity. It plays an irreplaceable role in lung cancer treatment.展开更多
Objective： Escape from the body＇s immune response is a basic characteristic of lung cancer, and indoleamine-2,3-dioxygenase （IDO） plays a key role in mediating immune escape of non-small-cell lung cancer, which le...Objective： Escape from the body＇s immune response is a basic characteristic of lung cancer, and indoleamine-2,3-dioxygenase （IDO） plays a key role in mediating immune escape of non-small-cell lung cancer, which leads to recurrence and metastasis. Feiji Recipe, a compound Chinese herbal medicine, has the effect of stabilizing lesions and prolonging survival in patients with lung cancer. The purpose of this study was to investigate the mechanisms underlying the anticancer properties of Feiji Recipe. Methods： An orthotopic transplant model of mouse Lewis lung cancer, with stable expression of IDO gene, was established in C57BL/6 mice. Optical imaging was used to observe the effects of Feiji Recipe in the treatment of lung cancer in vivo. The effects of Feiji Recipe on the proliferation of mouse Lewis lung cancer cell line 2LL, 2LL-enhanced green fluorescent protein （2LL-EGFP） and 2LL-EGFP-IDO were investi- gated, and the apoptosis of T-cells was examined by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetra zolium bromide using flow cytometry. Chemical composition of Feiji Recipe was validated by high- performance liquid chromatography. Results： Compared to the control group, the survival of animals treated with Feiji Recipe was significantly prolonged （P〈0.0074）, and the IDO protein level decreased （P=0.0072）; moreover, the percentages of CD4＋CD25＋ T-cells and Foxp3＋ T-cells were significantly decreased （P〈0.05）. The molecular mechanism of Feiji Recipe against lung cancer may relate to the regulation of immune cells, such as T-cells and reg- ulatory T-cells. Conclusion： The molecular mechanism of Feiji Recipe in treatment of lung cancer is to restore the function of T-cells in the cancer microenvironment through interfering with the IDO pathway.展开更多
背景与目的原发性支气管肺癌是最常见的恶性肿瘤,带给患者巨大的躯体病痛和情绪障碍,而且明显降低患者的生存质量,本实验旨在研究疏肝肺积方结合心理干预对原发性肺癌患者生存质量、体能状态的影响。方法将118例原发性非小细胞肺癌患者...背景与目的原发性支气管肺癌是最常见的恶性肿瘤,带给患者巨大的躯体病痛和情绪障碍,而且明显降低患者的生存质量,本实验旨在研究疏肝肺积方结合心理干预对原发性肺癌患者生存质量、体能状态的影响。方法将118例原发性非小细胞肺癌患者随机分为两组:疏肝肺积方结合心理干预加化疗组(联合治疗组57例)和单纯化疗组(61例)。联合治疗组采用疏肝肺积方和心理干预与化疗的联合应用;单纯化疗组仅接受化疗,共完成2次化疗,为1个总疗程。采用欧洲癌症研究与治疗组织生命质量核心量表(European Organization for Research and Treatment of Cancer QoL Questionnaire,EORTC QLQ-C30)和肺癌患者生命质量测定特异性模块(EORTC QLQ-LC13)测定生存质量,卡氏评分(Karnofsky performance status,KPS)和ECOG评分(East Cooperative Oncology Group performance status)测定体能状态。结果联合治疗组生理、角色、情感、认知、社会功能以及总健康状况领域的评分比单纯化疗组的评分高,生存质量更好,且具有统计学意义(P<0.01);联合治疗组疲倦、恶心呕吐、疼痛、气促、失眠、食欲丧失、便秘症状以及肺癌特异症状评分比单纯化疗组的评分低,缓解更明显,且具有统计学意义(P<0.01);联合治疗组卡氏评分和ECOG评分提高和稳定的患者多于单纯化疗组,且具有统计学意义(P<0.01)。结论疏肝肺积方结合心理干预能缓解肺癌患者的临床症状、改善体能状态、提高生存质量,具有较好的临床疗效。展开更多
文摘Lung cancer is the leading cause of cancer-related mortality in the world. Although surgery, chemotherapy and radiotherapy can improve the survival rate l, cancers still affect people's quality of life. Feiji Recipe is an empirical formula and has been used as a treatment for non-small cell lung cancer(NSCLC) for many years. This thesis collects data of the etiology and pathogenesis of Feiji Recipe, clinical research and animal experiments,and provide suggestive evidence of Feiji Recipe for treating NSCLC. During the treatment, the characteristic of holism and personalized treatment of traditional Chinese medicine(TCM)is proved, which also demonstrated that it can relieve patient's clinical syndrome, improve their quality of life and enhance the body immunity. It plays an irreplaceable role in lung cancer treatment.
基金supported by National Natural Science Foundation of China(No.81173224 and 81373621)Shanghai Municipal Committee of Science and Technology(No.11ZR1437000)+3 种基金the Science and Technology Commission of Shanghai Municipality(No.12401905700)Longhua Medical Project(D-11)(all to Jian-hui Tian)State Administration of Traditional Chinese Medicine Clinical Research Base Special Project(No.JDZX2012125)(He-gen Li)Action Plan of Shanghai Chinese Medicine for Three Years(No.ZY3-CCCX-2-1002)(Zhen Xiao)
文摘Objective： Escape from the body＇s immune response is a basic characteristic of lung cancer, and indoleamine-2,3-dioxygenase （IDO） plays a key role in mediating immune escape of non-small-cell lung cancer, which leads to recurrence and metastasis. Feiji Recipe, a compound Chinese herbal medicine, has the effect of stabilizing lesions and prolonging survival in patients with lung cancer. The purpose of this study was to investigate the mechanisms underlying the anticancer properties of Feiji Recipe. Methods： An orthotopic transplant model of mouse Lewis lung cancer, with stable expression of IDO gene, was established in C57BL/6 mice. Optical imaging was used to observe the effects of Feiji Recipe in the treatment of lung cancer in vivo. The effects of Feiji Recipe on the proliferation of mouse Lewis lung cancer cell line 2LL, 2LL-enhanced green fluorescent protein （2LL-EGFP） and 2LL-EGFP-IDO were investi- gated, and the apoptosis of T-cells was examined by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetra zolium bromide using flow cytometry. Chemical composition of Feiji Recipe was validated by high- performance liquid chromatography. Results： Compared to the control group, the survival of animals treated with Feiji Recipe was significantly prolonged （P〈0.0074）, and the IDO protein level decreased （P=0.0072）; moreover, the percentages of CD4＋CD25＋ T-cells and Foxp3＋ T-cells were significantly decreased （P〈0.05）. The molecular mechanism of Feiji Recipe against lung cancer may relate to the regulation of immune cells, such as T-cells and reg- ulatory T-cells. Conclusion： The molecular mechanism of Feiji Recipe in treatment of lung cancer is to restore the function of T-cells in the cancer microenvironment through interfering with the IDO pathway.
文摘背景与目的原发性支气管肺癌是最常见的恶性肿瘤,带给患者巨大的躯体病痛和情绪障碍,而且明显降低患者的生存质量,本实验旨在研究疏肝肺积方结合心理干预对原发性肺癌患者生存质量、体能状态的影响。方法将118例原发性非小细胞肺癌患者随机分为两组:疏肝肺积方结合心理干预加化疗组(联合治疗组57例)和单纯化疗组(61例)。联合治疗组采用疏肝肺积方和心理干预与化疗的联合应用;单纯化疗组仅接受化疗,共完成2次化疗,为1个总疗程。采用欧洲癌症研究与治疗组织生命质量核心量表(European Organization for Research and Treatment of Cancer QoL Questionnaire,EORTC QLQ-C30)和肺癌患者生命质量测定特异性模块(EORTC QLQ-LC13)测定生存质量,卡氏评分(Karnofsky performance status,KPS)和ECOG评分(East Cooperative Oncology Group performance status)测定体能状态。结果联合治疗组生理、角色、情感、认知、社会功能以及总健康状况领域的评分比单纯化疗组的评分高,生存质量更好,且具有统计学意义(P<0.01);联合治疗组疲倦、恶心呕吐、疼痛、气促、失眠、食欲丧失、便秘症状以及肺癌特异症状评分比单纯化疗组的评分低,缓解更明显,且具有统计学意义(P<0.01);联合治疗组卡氏评分和ECOG评分提高和稳定的患者多于单纯化疗组,且具有统计学意义(P<0.01)。结论疏肝肺积方结合心理干预能缓解肺癌患者的临床症状、改善体能状态、提高生存质量,具有较好的临床疗效。
