Traditional Chinese medicine Pseudostellaria heterophylla fibrous roots have been widely studied and applied in non-antibiotic clinical breeding of livestock and poultry. In vitro probiotic effect of P. heterophylla f...Traditional Chinese medicine Pseudostellaria heterophylla fibrous roots have been widely studied and applied in non-antibiotic clinical breeding of livestock and poultry. In vitro probiotic effect of P. heterophylla fibrous roots extract on feed probiotics Bacillus subtilis BD-K010 was studied, and the feasibility of solid fermentation of P. heterophylla fibrous roots was preliminarily evaluated. For in vitro probiotic effect, the increased concentration of P. heterophylla fibrous roots extract dependently increased the total biomass of B. subtilis BD-K010;1.0% P. heterophylla fibrous roots extract received the best effect, and the final p H of 1.0% experimental group was closer to neutral. Meantime, B. subtilis BD-K010 with optimum concentration of P. heterophylla fibrous roots showed significantly higher in vitro antibacterial effect than the control group(P<0.01),and the antibacterial effects on Escherichia coli and Staphylococcus aureus were improved by 51.99% and 63.16%, but it was ineffective to Salmonella. For solid fermentation, the profile of substrate complex and appendage flocculent structure on substrate surface at the end of fermenta-tion in experimental groups added with B. subtilis BD-K010 and cellulase plus BD-K010 were more complex;the live bacteria number, polysaccharide content and saponin content at fermentation end-point in two experimental groups were extremely higher than those in the control group(P<0.01).P. heterophylla fibrous roots extract had good in vitro probiotic effect on B. subtilis BD-K010 and promoted its antibacterial effect, and it is feasible to use probiotics for solid fermentation of P. heterophylla fibrous roots and to improve effective components. It is of great significance to further de-velop and utilize P. heterophylla fibrous roots resources in modern animal husbandry.展开更多
This study determined the effects of selenium on the growth of Fusorium strains and the effects of products extracted from the fungal cultures on relevant indicators of chondrocytes injury.
Due to the large-scale production and wide applications, many nanoparticles(NPs) enter wastewater treatment plants and accumulate in activated sludge. It is reported that titanium dioxide(Ti O2) NPs show severe damage...Due to the large-scale production and wide applications, many nanoparticles(NPs) enter wastewater treatment plants and accumulate in activated sludge. It is reported that titanium dioxide(Ti O2) NPs show severe damage to many model microbes. However, it is still unknown whether the long-term(e.g., 100 d) presence of Ti O2 NPs would affect the performance of sludge fermentation. In this study, long-term exposure experiments(105 d)were conducted to investigate the potential risk of Ti O2 NPs to sludge fermentation system. It is found that the presence of environmentally relevant [6 mg·(g TSS)-1] and higher [150 mg·(g TSS)-1] concentrations of Ti O2 NPs does not affect methane production from sludge fermentation. The analysis of fluorescence in situ hybridization indicates that these concentrations of Ti O2 NPs present marginal influences on abundances of bacteria and methanogenic archaea in sludge fermentation system. The viability of sludge microorganisms and activities of key enzymes related to methane production such as protease, acetate kinase, and coenzyme F420 are unchanged by the long-term presence of 6 and 150 mg·(g TSS)-1of Ti O2 NPs. Further investigations reveal that the insolubility of NPs and the protection role of sludge extracellular polymeric substances are the main reasons for the marginal influence of Ti O2 NPs on sludge fermentation.展开更多
基金Supported by Innovation Fund Project of Fujian Provincial Department of Science and Technology(2018C0041)Soft Science Project of Fujian Provincial Intellectual Property Office(2018ER019)
文摘Traditional Chinese medicine Pseudostellaria heterophylla fibrous roots have been widely studied and applied in non-antibiotic clinical breeding of livestock and poultry. In vitro probiotic effect of P. heterophylla fibrous roots extract on feed probiotics Bacillus subtilis BD-K010 was studied, and the feasibility of solid fermentation of P. heterophylla fibrous roots was preliminarily evaluated. For in vitro probiotic effect, the increased concentration of P. heterophylla fibrous roots extract dependently increased the total biomass of B. subtilis BD-K010;1.0% P. heterophylla fibrous roots extract received the best effect, and the final p H of 1.0% experimental group was closer to neutral. Meantime, B. subtilis BD-K010 with optimum concentration of P. heterophylla fibrous roots showed significantly higher in vitro antibacterial effect than the control group(P<0.01),and the antibacterial effects on Escherichia coli and Staphylococcus aureus were improved by 51.99% and 63.16%, but it was ineffective to Salmonella. For solid fermentation, the profile of substrate complex and appendage flocculent structure on substrate surface at the end of fermenta-tion in experimental groups added with B. subtilis BD-K010 and cellulase plus BD-K010 were more complex;the live bacteria number, polysaccharide content and saponin content at fermentation end-point in two experimental groups were extremely higher than those in the control group(P<0.01).P. heterophylla fibrous roots extract had good in vitro probiotic effect on B. subtilis BD-K010 and promoted its antibacterial effect, and it is feasible to use probiotics for solid fermentation of P. heterophylla fibrous roots and to improve effective components. It is of great significance to further de-velop and utilize P. heterophylla fibrous roots resources in modern animal husbandry.
基金funded by the Natural Science Basic Research Plan of Shaanxi Province,China(2014JM4170)the Department of disease control of Shaanxi Health and Family Planning Commission,China(2010/2012)
文摘This study determined the effects of selenium on the growth of Fusorium strains and the effects of products extracted from the fungal cultures on relevant indicators of chondrocytes injury.
基金Supported by the National Hi-Tech Research and Development Program of China(863Program)(2011AA060903)the National Natural Science Foundation of China(41301558and 51278354)Shanghai Tongji Gao Tingyao Environmental Science & Technology Development Foundation(STGEF)
文摘Due to the large-scale production and wide applications, many nanoparticles(NPs) enter wastewater treatment plants and accumulate in activated sludge. It is reported that titanium dioxide(Ti O2) NPs show severe damage to many model microbes. However, it is still unknown whether the long-term(e.g., 100 d) presence of Ti O2 NPs would affect the performance of sludge fermentation. In this study, long-term exposure experiments(105 d)were conducted to investigate the potential risk of Ti O2 NPs to sludge fermentation system. It is found that the presence of environmentally relevant [6 mg·(g TSS)-1] and higher [150 mg·(g TSS)-1] concentrations of Ti O2 NPs does not affect methane production from sludge fermentation. The analysis of fluorescence in situ hybridization indicates that these concentrations of Ti O2 NPs present marginal influences on abundances of bacteria and methanogenic archaea in sludge fermentation system. The viability of sludge microorganisms and activities of key enzymes related to methane production such as protease, acetate kinase, and coenzyme F420 are unchanged by the long-term presence of 6 and 150 mg·(g TSS)-1of Ti O2 NPs. Further investigations reveal that the insolubility of NPs and the protection role of sludge extracellular polymeric substances are the main reasons for the marginal influence of Ti O2 NPs on sludge fermentation.
