To study the effect of Green plum citron double strain fermentation filtrate(DSF)on the proliferation rate of HaCaT keratinocytes by CCK8 assay.The effect of DSF on the mRNA transcription levels of filagolin(FLG),lori...To study the effect of Green plum citron double strain fermentation filtrate(DSF)on the proliferation rate of HaCaT keratinocytes by CCK8 assay.The effect of DSF on the mRNA transcription levels of filagolin(FLG),loricrin(LOR),and occludin 1(CLD1)in HaCaT cells in sodium dodecyl sulfate(SLS)model was investigated by qPCR.The effect of DSF on the content of TNF-αin the supernatant of HaCaT cells in the model was investigated by Elisa.The results showed that DSF had no toxicity to HaCaT cells,but it could increase the mRNA transcription levels of FLG,LOR and CLD1 in HaCaT cells in the model,and reduce the content of TNF-αin the supernatant of HaCaT cells in the model.DSF had a certain effect on skin care of strengthening skin barrier.展开更多
The internal mechanism of organizational learning and knowledge increase is very similar to the mechanism of biotic ferment, so we creatively use Knowledge Convening ＆ Fermenting Model （KCFM） to explain the origina...The internal mechanism of organizational learning and knowledge increase is very similar to the mechanism of biotic ferment, so we creatively use Knowledge Convening ＆ Fermenting Model （KCFM） to explain the origination and mechanism of knowledge increase, espec）ally clarifying that the original substance is the key factor to cause organizational learning and knowledge innovation, We also thoroughly analyze the helix process for knowledge bacterial strain to impel knowledge increase.展开更多
文摘To study the effect of Green plum citron double strain fermentation filtrate(DSF)on the proliferation rate of HaCaT keratinocytes by CCK8 assay.The effect of DSF on the mRNA transcription levels of filagolin(FLG),loricrin(LOR),and occludin 1(CLD1)in HaCaT cells in sodium dodecyl sulfate(SLS)model was investigated by qPCR.The effect of DSF on the content of TNF-αin the supernatant of HaCaT cells in the model was investigated by Elisa.The results showed that DSF had no toxicity to HaCaT cells,but it could increase the mRNA transcription levels of FLG,LOR and CLD1 in HaCaT cells in the model,and reduce the content of TNF-αin the supernatant of HaCaT cells in the model.DSF had a certain effect on skin care of strengthening skin barrier.
基金This paper is supported by National Nature Science Foundation of China （NSFC） and Ph.D. research fund.
文摘The internal mechanism of organizational learning and knowledge increase is very similar to the mechanism of biotic ferment, so we creatively use Knowledge Convening ＆ Fermenting Model （KCFM） to explain the origination and mechanism of knowledge increase, espec）ally clarifying that the original substance is the key factor to cause organizational learning and knowledge innovation, We also thoroughly analyze the helix process for knowledge bacterial strain to impel knowledge increase.
