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Growth factors and fetal lung development mediated by mechanical forces 被引量:1
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作者 Juan Sanchez-Esteban 《World Journal of Respirology》 2013年第3期44-47,共4页
Incomplete development of the lung secondary to extreme prematurity or pulmonary hypoplasia causes significant morbidity and mortality during the neonatal period. Currently, the management is primarily supportive with... Incomplete development of the lung secondary to extreme prematurity or pulmonary hypoplasia causes significant morbidity and mortality during the neonatal period. Currently, the management is primarily supportive with no specific treatment to stimulate the growth and development of the lung. Mechanical forces generated inside the fetal lung by constant distention pressure and "breathing-like movements" are a major determinant of fetal lung development. However, the mechanisms by which lung cells sense these mechanical signals to promote lung development are not well-defined. Tracheal ligation has been used not only experimentally but also in human fetuses affected by severe congenital diaphragmatic hernia to stimulate lung growth and decrease the degree of pulmonary hypoplasia. Past investigations suggested that the increase of intratracheal pressure after tracheal ligation releases soluble factors that are critical for lung development. Studies from our laboratory have shown that mechanical strain of fetal type Ⅱ epithelial cells, simulating mechanical forces in utero, promotes differentiation via release of epidermal growth factor receptor ligands heparin binding epidermal growth factor-like growth factor and transforming growth factor alpha. The identification of growth factors released by mechanical forces that are importantfor normal lung development could lead to novel treatments to accelerate lung development. 展开更多
关键词 Mechanical FORCES lung development TRACHEAL LIGATION growth FACTORS
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Association of Insulin-like Growth Factors with Lung Development in Neonatal Rats 被引量:3
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作者 刘汉楚 常立文 +4 位作者 容志惠 祝华平 张谦慎 陈红兵 李文斌 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2004年第2期162-165,共4页
To explore the relationship between Insulin-like growth factor (IGF)- Ⅰ, -Ⅱ and lung development in neonatal rats. 80 timed pregnant Sprague-Dawley ( SD) rats were randomly divided into 4 groups (n=20): group A (Con... To explore the relationship between Insulin-like growth factor (IGF)- Ⅰ, -Ⅱ and lung development in neonatal rats. 80 timed pregnant Sprague-Dawley ( SD) rats were randomly divided into 4 groups (n=20): group A (Control group), group B (Dexamethasone (DEX) 1 group), group C (DEX 2 group), group D (retinoic acid (RA) group). 20 pregnant rats in group A, B and D were injected subcutaneously or intraperitoneally with vehicle (NS), DEX, or RA respectively during gestational day 16 to 18. All newborn rats in group C were subcutaneously injected with DEX at day 1 to 3 after birth. The lung tissue was obtained at the following times: fetuses at gestational ages of 18, 20 and 21 days, and 1, 3, 5, 7, 10, 14 and 21 days after birth. Lung tissues were used for histopathological study, the polypeptides analysis of IGF-Ⅰ, -Ⅱ (immunohistochemistry and Western blot) and mRNA analysis ( RT- PCR). The results showed that the strongest expression of IGF-Ⅰ in group A and D occurred at ages of 5-7 days (alveolar stage). The stronger their expressions, the better the alveolar develop. The peak stage of expression in group B occurred earlier, on the day 3 after birth. Compared with group A, the expression of IGF-Ⅰ during gestation age of 18 days to age of 3 days in group B were significantly higher (P<0.01), but significantly lower at other time points (P<0.01). The expression of IGF-Ⅰ was lower in group C all the time and always higher in group D than those in group A (P<0.01). The peak expression of IGF-Ⅱ took place at the gestation age of 18 days, then gradually dropped to trace. During 18 days of gestation to age of 3 days, the expression of IGF-Ⅱ in group B was significantly higher than that in group A (P<0.01). No difference was found among all other groups. The change in the expression of IGF-Ⅰ, -Ⅱ mRNA in all 4 groups was similar to that of their polypeptides. The results suggested that there is a close linking between IGF-Ⅰ, -Ⅱ and lung development in newborns. The IGF-Ⅱ works at early stage and the that of IGF-Ⅰ works at the stage of new septa formation and alveoli maturation. The stronger their expressions, the more mature the lung development. 展开更多
关键词 NEONATE lung development insulin-like growth factor
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Effect of Retinoic acid on Platelet-derived Growth Factorand Lung Development in Newborn Rats
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作者 陈红兵 常立文 +4 位作者 刘汉楚 容志惠 祝华平 张谦慎 李文斌 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2004年第3期226-228,共3页
Summary: The influence of platelet-derived growth factor (PDGF) on lung development in newborn rats and the effect of retinoic acid (RA) on PDGF in lung development were investigated. Newborn Sprague-Dawley (SD) rats ... Summary: The influence of platelet-derived growth factor (PDGF) on lung development in newborn rats and the effect of retinoic acid (RA) on PDGF in lung development were investigated. Newborn Sprague-Dawley (SD) rats were randomly assigned to two groups: control group and RA group. The rats in RA group was intraperitoneally injected with all trans-retinoic acid (500 μg/kg every day) for consecutive 3 days after birth, while those in the control group were not subjected to intervention. Immunohistochemical assay was performed to locate the expression of PDGF. mRNA levels of PDGF were measured by reverse transcription polymerase chain reaction (RT-PCR) at age of 1, 3, 5, 7, 10, 14, 21 days. The method of radial alveolar counts (RAC) was used to measure the amount of the alveoli of the lungs. It was found that with increasing days, levels of PDGF-A and PDGF-B changed to verying degrees. RA could elevate significantly the expression levels of PDGF-A mRNA and protein (P<0.01), but not affect the expression levels of PDGF-B mRNA and protein markedly (P>0.05). It is suggested that PDGF might play an important role in lung development. RA can stimulate lung development through increasing the expression levels of PDGF-A mRNA and protein. 展开更多
关键词 platelet-derived growth factor retinoic aicd lung development newborn rats
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Expression of Lung Surfactant Proteins SP-B and SP-C and Their Modulating Factors in Fetal Lung of FGR Rats 被引量:6
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作者 邓飞涛 欧阳为相 +2 位作者 葛良芳 张莉 柴新群 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2015年第1期122-128,共7页
This study investigated the expression of lung surfactant proteins SP-B and SP-C, and their modulating factors TTF-1 and PLAGL2 in the fetal lung of rats with fetal growth restriction(FGR). The rat FGR model was est... This study investigated the expression of lung surfactant proteins SP-B and SP-C, and their modulating factors TTF-1 and PLAGL2 in the fetal lung of rats with fetal growth restriction(FGR). The rat FGR model was established by prenatal hypoxia in the first stage of pregnancy, 180 rats for experiment served as hypoxia group, and 197 healthy rats served as normal control group. The FGR incidence in hypoxia was compared with that in normal control group. The histological changes in the fetal lung were observed under the light microscope and electronic microscope in two groups. The SP-B, SP-C, TTF-1 and PLAGL2 proteins were determined in the fetal lung of two groups immunohistochemically. The expression levels of SP-B, SP-C, TTF-1 and PLAGL2 protein and m RNA in the fetal lung of two groups were detected by using Western blotting and RT-PCR respectively. The FGR rat model was successfully established by using hypoxia. Pathologically the fetal lung developed slowly, and the expression levels of SP-B, SP-C, TTF-1 and PLAGL2 protein and mR NA in the fetal lung were significantly reduced in hypoxia group as compared with those in normal control group. It was suggested that maternal hypoxia in the first stage of pregnancy could induce FGR, and reduce the expression of SP-B and SP-C, resulting in the disorder of fetal lung development and maturation. 展开更多
关键词 SP-B SP-C PLAGL2 TTF-1 fetal growth restriction lung development real-time PCR Western blot immunohistochemistry
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Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction 被引量:9
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作者 Jing Liu Xiaofeng Wang +3 位作者 Ying Liu Na Yang Jing Xu Xiaotun Ren 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第23期2190-2197,共8页
From pregnancy to parturition, Sprague-Dawley rats were daily administered a low protein diet to establish a model of intrauterine growth restriction. From the 12th day of pregnancy, 300 mg/kg taurine was daily added ... From pregnancy to parturition, Sprague-Dawley rats were daily administered a low protein diet to establish a model of intrauterine growth restriction. From the 12th day of pregnancy, 300 mg/kg taurine was daily added to food until spontaneous delivery occurred. Brain tissues from normal neonatal rats at 6 hours after delivery, neonatal rats with intrauterine growth restriction, and neo- natal rats with intrauterine growth restriction undergoing taurine supplement were obtained for fur- ther experiments. The terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labeling assay revealed that the number of apoptotic cells in the brain tissue of neonatal rats with intrauterine growth restriction significantly increased. Taurine supplement in pregnant rats reduced cell apoptosis in brain tissue from neonatal rats with intrauterine growth restriction. Immu- nohistochemical staining revealed that taurine supplement increased glial cell line-derived neuro- trophic factor expression and decreased caspase-3 expression in the cerebral cortex of intrauterine growth-restricted fetal rats. These results indicate that taurine supplement reduces cell apoptosis through the glial cell line-derived neurotrophic factor-caspase-3 signaling pathway, resulting in a protective effect on the intrauterine growth-restricted fetal rat brain. 展开更多
关键词 neural regeneration intrauterine growth restriction fetal rats brain neural cells TAURINE cell apop-tosis glial cell line-derived neurotrophic factor caspase-3 neural development grants-supportedpaper NEUROREGENERATION
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Expression of Lung Surfactant Proteins SP-B and SP-C and Their Regulatory Factors in Fetal Lung of GDM Rats 被引量:4
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作者 Qing-miao ZHANG Wei-xiang OUYANG +1 位作者 Xin-qun CHAI Fei-tao DENG 《Current Medical Science》 SCIE CAS 2018年第5期847-852,共6页
This study investigated the expression of lung surfactant proteins (SP-B and SP-C),and regulatory factors [forkhead box A2(FOXA2)and nitrolyogenic FOXA2 (N-FOXA2)]in the fetal lung of rats with gestational diabetes me... This study investigated the expression of lung surfactant proteins (SP-B and SP-C),and regulatory factors [forkhead box A2(FOXA2)and nitrolyogenic FOXA2 (N-FOXA2)]in the fetal lung of rats with gestational diabetes mellitus (GDM)in order to study the mechanism of pulmonary dysplasia.The rat GDM model was established by using streptozotocin intraperitoneally in the first stage of pregnancy.There were 10 rats in the GDM group,and 10 healthy rats in normal control group without any treatment.Fetal lungs of two groups were taken at day 21 of pregnancy.Blood glucose levels of maternal rats and fetal rats were measured by Roche blood glucose meter.The histological changes in the fetal lung were observed under the light microscope in both groups.The SP-B,SP-C and FOXA2were determined in the fetal lung of two groups immunohistochemically. The expression levels of SP-B,SP-C,total FOXA2,FOXA2 in nucleus (n-FOXA2), N-FOXA2 proteins were detected by Western blotting,and the relative expression levels of SP-B,SP-C,FOXA2 mRNA in the fetal lung of two groups were detected by RT-PCR.The results showed that blood glucose levels of maternal rats and fetal rats in GDM group were higher than those in control group.The light microscope revealed fetal lung development retardation in GDM group.The expression of SP-B and SP-C in GDM group was significantly reduced as compared with control group (P<0.05).As compared with control group,the n-FOXA2 expression was significantly decreased in the fetal lung tissue,and N-FOXA2 was significantly increased in control group (P<0.05),but there was no significant changes in the total FOXA2(P>0.05).It was concluded that GDM can cause fetal lung development and maturation disorders,and FOXA2 in fetal lung tissue decreases while nitrocellulose FOXA2 increases. 展开更多
关键词 nitrolyogenic FOXA2 fetal lung development GESTATIONAL diabetes MELLITUS
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Cigarette Smoke Induces Apoptosis by Activation of Caspase-3 in Isolated Fetal Rat Lung Type II Alveolar Ep-ithelial Cells <i>in Vitro</i>
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作者 Asra Ahmed James A. Thliveris +3 位作者 Anthony Shaw Michael Sowa James Gilchrist J. Elliott Scott 《Open Journal of Respiratory Diseases》 2013年第1期4-12,共9页
Smoking during pregnancy is a major source of fetal exposure to numerous harmful agents present in tobacco smoke. Lung development involves complex biochemical processes resulting in dramatic changes which continue ev... Smoking during pregnancy is a major source of fetal exposure to numerous harmful agents present in tobacco smoke. Lung development involves complex biochemical processes resulting in dramatic changes which continue even after birth. In addition to type I cells which form the blood-air barrier, type II alveolar epithelial (AE) cells have important and diverse functions related to immunological protection and stabilization of the alveolus through synthesis and secretion of the pulmonary surfactant. Apoptosis or programmed cells death is an important physiological process during lung embryogenesis and for the proper maintenance of homeostasis. Caspases are proteases that play important roles in regulating apoptosis. Caspase-3 is the key executioner caspase in the cascade of events leading to cell death by apoptosis. We explored the hypothesis that cigarette smoke extract (CSE) induces apoptosis in fetal rat lung type II AE cells by activation of caspase-3. To analyze these factors, isolated fetal rat lung type II AE cells were used. The cells were exposed to different concentrations of CSE (5%, 10% or 15%) (v/v) for 60 min. The results of the present study showed that CSE induced apoptosis in fetal rat lung type II AE cells with a significant increase (p 0.05) in caspase-3 activity and decrease in cell proliferation at CSE concentrations of 10% and 15% (v/v). These observations indicate that cigarette smoke extract induces apoptosis by activation of caspase-3 in fetal rat lung type II AE cells in a dose-dependent manner and may potentially alter the regulated development of the lung and the appearance of the surfactant-producing type II alveolar cells which are critical for the establishment of adequate gas exchange at birth. 展开更多
关键词 Cigarette Smoke TOXICITY fetal Rat lung Type II ALVEOLAR Cells APOPTOSIS Protease CASPASE-3 lung development developmental TOXICITY Maternal Smoking
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Fetal weight growth trajectories and childhood development:A population-based cohort study
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作者 Xinmei Chen Hongxiu Liu +16 位作者 Aifen Zhou Feng Jin Chufeng Jing Yuanyuan Li Wei Xia Linda GKahn Ya Xie Xingliang Xiang Shuting Cao Wenxin Zhang Gaga Mahai Zhongqiang Cao Han Xiao Chao Xiong Wei Li Hanzeng Li Shunqing Xu 《Science Bulletin》 SCIE EI CAS CSCD 2024年第21期3404-3414,共11页
This study aimed to investigate whether fetal growth trajectories(FGTs)could predict early childhood development,indicate intrauterine metabolic changes,and explore potential optimal and suboptimal FGTs.FGTs were deve... This study aimed to investigate whether fetal growth trajectories(FGTs)could predict early childhood development,indicate intrauterine metabolic changes,and explore potential optimal and suboptimal FGTs.FGTs were developed by using an unsupervised machine-learning approach.Children’s neurodevelopment,anthropometry,and respiratory outcomes in thefirst 6 years of life were assessed at different ages.In a subgroup of participants,we conducted a metabolomics analysis of cord blood to reveal the metabolic features of FGTs.We identified 6 FGTs:early decelerating,early decelerating with late catch-up growth,early accelerating,early accelerating with late medium growth,late decelerating,and late accelerating.The early accelerating with late medium growth pattern might be the optimal FGT due to its associations with better psychomotor development,mental development,intelligence quotient,and lung function and a lower risk of behaviour and respiratory problems.Compared with the optimal FGT,early decelerating and late decelerating FGTs were associated with poor neurodevelopment and lung function,while early accelerating FGT was associated with more severe autistic symptoms,poor lung function,and increased risks of overweight/obesity.Metabolic alterations were enriched in amino acid metabolism for early decelerating and late decelerating FGTs,whereas altered metabolites were enriched in lipid metabolism for early accelerating FGT.Thesefindings suggest that FGTs are predictors of early life development and may indicate intrauterine adaptive metabolism.The discovery of optimal and suboptimal FGTs provides potential clues for the early identification and intervention of fetal origin dysplasia or disease,but further research on related mechanisms is still needed. 展开更多
关键词 fetal growth trajectory Children development NEUROdevelopment COHORT
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Effect of epidermal growth factor and dexamethasone on fetal rat lung development 被引量:4
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作者 MA Lian WANG Ai-hong +4 位作者 Law Frieda HE Hong-yan MA Gui-xia WANG Hong-wu LIN Li-min 《Chinese Medical Journal》 SCIE CAS CSCD 2009年第17期2013-2016,共4页
Background Epidermal growth factor (EGF), a mitogenic polypeptide that binds to cell surface receptors, is an important regulator of cell differentiation and fetal lung surfactant synthesis. We investigated the prev... Background Epidermal growth factor (EGF), a mitogenic polypeptide that binds to cell surface receptors, is an important regulator of cell differentiation and fetal lung surfactant synthesis. We investigated the preventive and therapeutic effects of EGF in respiratory distress syndrome, by administering EGF and dexamethasone (Dex) to mother rat before delivery. Methods Six female Sprague-Dawley (SD) rats were assigned to three groups (2 rats each); EGF or Dex was given to pregnant rats (EGF group and Dex group, respectively) from gestational day 16 to day 18 by intraperitoneal injection, while the group with normal saline injection was used as negative controls. Fetal rats were taken out of womb by hysterotomy on day 19 of pregnancy, then 24 fetal rats were randomly chosen from each group. Their body weights were measured, and pulmonary surfactant protein-A and -B (SP-A and SP-B) antigens were determined by immunohistochemical staining in each group. The histologic structure was examined under a light microscope, a light microscopic image system or an electron microscope. Results The expressions of SP-A and SP-B could be detected in each group. A significant difference was observed for SP-A and SP-B in the EGF and Dex groups compared with the control group (P 〈0.01). Image analysis showed that the relative values of air space area and interalveolar septa area in the EGF and Dex groups were significantly greater than those in the control group (P 〈0.01), while no significant difference was found between the two groups (P 〉0.05). The ultrastructural features of fetal lungs showed that the number of alveolar type [I cells containing lamellar bodies in the EGF and Dex groups was apparently increased compared with that in the control group. The mean body weight of fetus from the Dex group was smaller than that from the control group ((1.3192+0.0533) g, (1.3863_+0.0373) g), but there was no significant difference between the EGF group and the control group ((1.3986_+0.0730) g, (1.3863_+0.0373) g). Conclusions Maternal treatment with EGF and Dex on days 16-18 of gestation could promote morphogenesis and increase the surfactant levels in premature fetal lung. However, maternal treatment with Dex, not EGF, decreased the body weight. Chin Med J 2009; 122(17):2013-2016 展开更多
关键词 epidermal growth factor DEXAMETHASONE fetal lung
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Effect of Retinoic Acid on Lung Injury in Hyperoxia-Exposed Newborn Rats 被引量:2
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作者 常立文 容志惠 +1 位作者 张谦慎 钱莉玲 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2003年第1期71-74,共4页
To investigate whether treatment with retinoic acid (RA) could improve level of lung alveolarization and influence lung collagen in newborn rats exposed to hyperoxia, newborn Sprague-Dawley rats aged 2 days were ra... To investigate whether treatment with retinoic acid (RA) could improve level of lung alveolarization and influence lung collagen in newborn rats exposed to hyperoxia, newborn Sprague-Dawley rats aged 2 days were randomly assigned to 8 groups:(1) air, (2) O 2, (3) air+NS, (4) O 2+NS, (5) air+dex, (6) O 2+dex, (7) air+RA and (8) O 2+RA. Group 2, 4 6 and 8 were kept in chambers containing 85 % oxygen, the values were checked 3 times a day. The other 4 groups were exposed to room air. Level of alveolarization and lung collagen were analyzed at age of 14 or 21 days through radial alveolar counts, alveolar airspace measurements, type Ⅰ, Ⅲ collagen immunohistochemical methods (SP method) and image processing system. Transforming growth factor-β receptors and procollagen mRNA accumulation were examined at age of 14 days through immunohistochemical methods and in situ hybridization. Our results showed that radial alveolar counts were increased and distal airspace was enlarged in group 8. TypeⅠcollagen was markedly increased, and transforming growth factor-β receptors and procollagen mRNA were decreased by retinoic acid in bronchial epithelial cells, alveolar epithelial cells and alveolar intersitium. It is concluded that retinoic acid can partially reverse lung development arrest during exposure to hyperoxia by increasing lung collagen. 展开更多
关键词 retinoic acid HYPEROXIA lung development COLLAGEN transforming growth factor-β receptors
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Effects of Jinye Baidu Granule (金叶败毒颗粒) on Fetal Growth and Development with Maternal Active Human Cytomegalovirus Infection 被引量:1
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作者 姜宏 陈素华 闻良珍 《Chinese Journal of Integrative Medicine》 SCIE CAS 2006年第4期250-254,共5页
To evaluate the effects of Jinye Baidu Granule (金叶败毒颗粒, JYBDG), a traditional Chinese medicine compound prescription, on fetal growth and development with maternal active human cytomegalovirus infection. Meth... To evaluate the effects of Jinye Baidu Granule (金叶败毒颗粒, JYBDG), a traditional Chinese medicine compound prescription, on fetal growth and development with maternal active human cytomegalovirus infection. Methods: A prospective, randomized and controlled trial was adopted during January 1996 to June 2002. From the pregnant women with an abnormal pregnant history, 240 cases were screened to be infected by human cytomegalovirus (HCMV) by enzyme-linked immunoabsorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). They were assigned according to the random number table to two groups. The 122 cases in the treatment group were administrated with JYBDG, one package each time, three times a day for two continuous weeks, while the other 118 in the control group did not receive any treatment. The negative conversion rate of both HCMV-IgM and HCMV late mRNA, the positive rate of HCMV-DNA in placenta and the intrauterine transmission rate between the two groups were compared, and fetal growth and development in partial fetuses were also observed. Results: The negative conversion rate of both HCMV-IgM and HCMV late mRNA, the positive rate of HCMV-DNA in placenta and the intrauterine transmission rate in the treatment group were 77. 05% (94/122), 48.98% (48/98) and 21.74% (10/46) respectively, while those in the control group were 38. 14% (45/118), 67.50% (54/80) and 52.63% (20/38) respectively, all showing significant difference between the two groups (P〈0.01). Totally 35 normal infants and 11 abnormal infants were born in the treatment group, and the number in the control group was 20 and 18 respectively, and comparison between the two groups showed significant difference (P〈0.01). Six months of child birth, the scores of both mental development index (MDI) and psychomotor development index (PDI) of infants were higher in the treatment group (20 cases) than those in the control group (20 cases), but there was no significant difference between the two groups (P〉0.05). Conclusion: JYBDG could decrease the intrauterine transmission of HCMV and is beneficial to fetal growth and development. 展开更多
关键词 Chinese herbal medicine CYTOMEGALOVIRUS intrauterine infection fetal growth and development Jinye Baidu Granule
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葛根素对妊娠期糖尿病大鼠母体和胎儿的影响 被引量:1
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作者 钱坤 宋祎一 曹莉 《中国药理学通报》 CAS CSCD 北大核心 2024年第3期469-475,共7页
目的观察口服葛根素(puerarin,Pue)对妊娠期糖尿病(gestational diabetic mellitus,GDM)大鼠母体的药效及对其胎儿生长发育的影响,为Pue用于治疗GDM提供参考。方法给已孕母鼠尾静脉注射链脲佐菌素建立GDM大鼠模型,灌胃Pue治疗12 d,记录... 目的观察口服葛根素(puerarin,Pue)对妊娠期糖尿病(gestational diabetic mellitus,GDM)大鼠母体的药效及对其胎儿生长发育的影响,为Pue用于治疗GDM提供参考。方法给已孕母鼠尾静脉注射链脲佐菌素建立GDM大鼠模型,灌胃Pue治疗12 d,记录孕鼠体质量及流产情况,检测孕鼠给药治疗前后的空腹血糖,并分别于给药后的第5天和第10天检测母鼠糖耐量;母鼠怀孕第20天行剖腹产,检测胎鼠血糖含量,并观察胎鼠生长发育情况。测定胎鼠体质量、体长、尾长及胎盘和重要脏器的重量,计算脏器指数。结果与模型组相比,Pue可显著降低GDM孕鼠及胎鼠的空腹血糖,改善孕鼠糖耐量,有效缓解GDM导致的孕鼠体质量过度增加和胎鼠体质量过大的状况,降低流产率;并可逆转GDM引起的胎鼠脑、心、肝等脏器指数下降和肾脏脏器指数升高。结论口服Pue可缓解GDM母体及胎儿的高血糖状态,降低流产率,减少巨大儿的发生,促进胎儿重要脏器的发育。 展开更多
关键词 葛根素 妊娠期糖尿病 血糖 胎鼠 生长发育 宫内生长受限
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川芎嗪对实验性手术胎儿生长受限大鼠胎儿发育的影响及机制
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作者 信艳萍 王雅莉 +1 位作者 李瓅 崔雪梅 《西北药学杂志》 CAS 2024年第4期81-85,共5页
目的探讨川芎嗪对实验性手术胎儿生长受限(fetal growth restriction,FGR)大鼠胎儿发育及大鼠胎盘组织中可溶性血管内皮生长因子受体-1(soluble fms-like tyrosine kinase-1,sFlt-1)和胎盘生长因子(placenta growth factor,PLGF)表达水... 目的探讨川芎嗪对实验性手术胎儿生长受限(fetal growth restriction,FGR)大鼠胎儿发育及大鼠胎盘组织中可溶性血管内皮生长因子受体-1(soluble fms-like tyrosine kinase-1,sFlt-1)和胎盘生长因子(placenta growth factor,PLGF)表达水平的影响。方法将24只孕鼠随机分为正常组、模型组、川芎嗪组(80 mg·kg^(−1)),每组8只。除正常组外,其余2组于妊娠第5天开始用烟熏法建立FGR大鼠模型。妊娠第21天实施剖腹产,进行胎儿结局评估、检查胎儿生长发育情况并检测大鼠胎盘组织中sFlt-1、PLGF mRNA及蛋白的表达情况。结果与正常组比较,模型组大鼠胚胎吸收率、大鼠胎盘组织中sFlt-1 mRNA及蛋白的表达水平、活胎内脏畸形率和变异率、骨骼畸形率与变异率均显著升高(P<0.05),大鼠胎盘组织中PLGF mRNA及蛋白的表达水平、成活胎儿数量、活胎体质量及掌骨、跖骨、尾椎骨骨化率均显著降低(P<0.05);与模型组比较,川芎嗪组大鼠胚胎吸收率、大鼠胎盘组织中sFlt-1 mRNA及蛋白表达水平、活胎内脏畸形率和变异率、骨骼畸形率与变异率均显著降低(P<0.05),大鼠胎盘组织中PLGF mRNA及蛋白的表达水平、成活胎儿数量、活胎体质量及掌骨、跖骨、尾椎骨骨化率显著升高(P<0.05)。结论川芎嗪可以显著下调FGR孕鼠sFlt-1的表达水平,上调PLGF的表达水平,促进胎儿生长发育,改善胎儿生长受限。 展开更多
关键词 川芎嗪 实验性手术胎儿生长受限 胎儿发育 可溶性血管内皮生长因子受体-1 胎盘生长因子
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胎儿宫内及出生后生长轨迹研究进展
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作者 王亚新 马良坤 +3 位作者 孙崟 高劲松 刘娜娜 杨萱瑾 《中国妇幼健康研究》 2024年第3期81-87,共7页
胎儿出生体重是妊娠结束时评估胎儿生长状况的最直观指标,但其作为一个单一的衡量指标并不能反映整个妊娠期胎儿生长的动态变化,相似的出生体重可能沿着不同的生长轨迹获得。目前胎儿生长受限或巨大儿等诊断是基于估计胎儿体重或腹围的... 胎儿出生体重是妊娠结束时评估胎儿生长状况的最直观指标,但其作为一个单一的衡量指标并不能反映整个妊娠期胎儿生长的动态变化,相似的出生体重可能沿着不同的生长轨迹获得。目前胎儿生长受限或巨大儿等诊断是基于估计胎儿体重或腹围的横断面临界值判断的,而横断面测量可能会遗漏某些未能达到其潜能、但仍高于传统临界值的胎儿亚组,这些亚组的胎儿同样面临不良围产结局的风险。对胎儿生长进行纵向评估可能是解决这一临床问题的有效方法之一。本文从胎儿宫内及出生后生长轨迹的影响因素、不同轨迹下胎儿的远期健康结局等方面进行综述,以期为相关领域的进一步研究提供参考。 展开更多
关键词 胎儿生长轨迹 宫内发育 生命早期健康 生长发育 模型
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Foxp3在小鼠肺发育中的动态表达及意义 被引量:1
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作者 江健锋 卢红艳 +2 位作者 朱玥 何朗粤 朱莹 《江苏大学学报(医学版)》 CAS 2024年第2期132-137,共6页
目的:通过分析叉头样转录因子3(forkhead box protein 3,Foxp3)在小鼠肺发育过程中的表达及其与肺发育相关标志物之间的关系,探讨Foxp3在肺发育中的可能作用。方法:根据小鼠肺发育的进程,选取胎鼠及新生鼠分为6组,分别于孕17.5 d及出生... 目的:通过分析叉头样转录因子3(forkhead box protein 3,Foxp3)在小鼠肺发育过程中的表达及其与肺发育相关标志物之间的关系,探讨Foxp3在肺发育中的可能作用。方法:根据小鼠肺发育的进程,选取胎鼠及新生鼠分为6组,分别于孕17.5 d及出生后1、4、7、14、21 d留取肺组织,HE染色观察肺组织形态,免疫组织化学染色检测CD31含量并观察肺微血管密度。qRT-PCR检测Foxp3及肺表面活性蛋白C(surfactant protein C,SP-C)、血管内皮生长因子A(vascular endothelial growth factor A,VEGF-A)、血管生成素-1(angiopoietin 1,Ang-1)mRNA表达,蛋白质印迹法检测Foxp3及SP-C、VEGF-A、Ang-1蛋白表达。结果:肺组织内Foxp3 mRNA在孕17.5 d小管期表达最高,后呈现不断减少趋势。SP-C mRNA在小鼠出生后1 d表达最高,随后逐渐减弱,直至肺泡化晚期。VEGF-A、Ang-1 mRNA在孕17.5 d表达最高,之后呈现不断减少趋势,最终趋于稳定。Foxp3蛋白在小管期已有表达,且在小管期及囊泡期表达最高,其后逐渐趋于平稳,VEGF-A及Ang-1在小管期及囊泡期表达亦最高,后逐渐减少;SP-C表达于出生后1 d达到最高,随后逐渐减少,并于肺泡化中晚期趋于平稳。相关性分析显示,Foxp3与SP-C、VEGF-A及Ang-1存在相关性(r分别为0.661、0.630和0.738)。结论:Foxp3在胎肺期及出生后早期呈现动态表达,Foxp3在小管期及囊泡早中期的高表达与SP-C、VEGF-A及Ang-1呈正相关,提示Foxp3可能促进肺泡上皮细胞及肺血管内皮细胞的增殖,参与肺发育过程。 展开更多
关键词 肺发育 叉头样转录因子3 肺表面活性蛋白C 血管内皮生长因子A 血管生成素-1 调节性T细胞
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胎儿生长受限的病因及对患儿远期健康的影响
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作者 王雅慧 王艳 +1 位作者 王艳(审校) 裴飞 《国际妇产科学杂志》 CAS 2024年第2期152-156,共5页
胎儿生长受限(fetal growth restriction,FGR)是指胎儿在妊娠期内无法达到其预期的生长潜力,其是妊娠期常见且较复杂的并发症之一。FGR病因复杂多样,可能是由母体、胎儿或胎盘因素所引起。对于存在FGR的儿童和成年患者,长期的追踪研究... 胎儿生长受限(fetal growth restriction,FGR)是指胎儿在妊娠期内无法达到其预期的生长潜力,其是妊娠期常见且较复杂的并发症之一。FGR病因复杂多样,可能是由母体、胎儿或胎盘因素所引起。对于存在FGR的儿童和成年患者,长期的追踪研究揭示了其健康状况的不良后果。生长受限胎儿的出生体质量和身长明显落后于正常儿童,绝大部分患儿在生后早期即开始出现明显的生长追赶,但其存在更高的代谢问题风险。FGR常常伴随着一系列的远期并发症,如神经系统发育障碍和骨骼肌生长代谢异常等问题,甚至是在成年期时更易出现代谢综合征和心血管疾病,这对患儿的身体健康和生活质量产生了严重影响。综述FGR致病因素及对患儿远期健康的影响,以期为临床防治提供相关理论支持。 展开更多
关键词 胎儿生长迟缓 胚胎发育 神经发育障碍 肌肉骨骼发育 代谢综合征
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二甲双胍对妊娠期高血糖孕妇后代远期影响的研究进展
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作者 王世成 杨延冬 《中国卫生标准管理》 2024年第19期194-198,共5页
妊娠期高血糖是指孕前或孕期发生的不同程度的糖代谢紊乱,严重影响母婴结局。近些年来,妊娠期高血糖的发病率不断增加,妊娠期高血糖的孕妇在饮食及运动管理控制不佳时通常采用胰岛素及口服药物治疗。二甲双胍作为一种口服降糖药物,在降... 妊娠期高血糖是指孕前或孕期发生的不同程度的糖代谢紊乱,严重影响母婴结局。近些年来,妊娠期高血糖的发病率不断增加,妊娠期高血糖的孕妇在饮食及运动管理控制不佳时通常采用胰岛素及口服药物治疗。二甲双胍作为一种口服降糖药物,在降低孕妇血糖的同时,还能降低新生儿低血糖、巨大儿等不良事件的发生率,有效控制孕妇孕期体质量增长。二甲双胍治疗对母体是安全的,但与胰岛素治疗不同的是,二甲双胍能够通过胎盘进入胎儿体内,因此仍然存在二甲双胍治疗对胎儿远期预后影响的担忧。目前国内外对二甲双胍治疗的产妇后代长期生长发育影响程度尚不明确,本综述就二甲双胍作用机制及后代远期生长发育影响进行总结,并为妊娠期高血糖临床指南标准后期制定提供借鉴内容。 展开更多
关键词 二甲双胍 妊娠期高血糖 远期影响 母婴结局 胎儿发育 儿童 生长发育
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山羊胎期肺发育的形态学研究 被引量:6
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作者 郑月茂 徐永平 +2 位作者 卿素珠 蒲鹏 张涌 《畜牧兽医学报》 CAS CSCD 北大核心 2005年第2期158-165,共8页
对3~22周龄山羊胎儿肺进行了肉眼、光镜和透射电镜观察,结果表明:1.7~22周龄山羊胎儿肺的外部形态与胎龄无关,肺的外部形态以左二右四叶者为多见,肺的叶间裂和右肺副裂常不完整,以浆膜、肺组织或混合性组织(肺组织及浆膜)融合;2. 山... 对3~22周龄山羊胎儿肺进行了肉眼、光镜和透射电镜观察,结果表明:1.7~22周龄山羊胎儿肺的外部形态与胎龄无关,肺的外部形态以左二右四叶者为多见,肺的叶间裂和右肺副裂常不完整,以浆膜、肺组织或混合性组织(肺组织及浆膜)融合;2. 山羊胎儿肺的发育分为5个时期:胚胎期(3~5周) 肺芽分支形成主支气管,主支气管长度不断增长并萌芽出叶支气管,均衬以假复层柱状上皮.腺状期(6~12周) 以支气管树发育为主,小支气管衬以假复层和/或单层柱状上皮;终蕾呈腺状,上皮细胞由假复层柱状逐渐变为单层柱状,胞核向细胞顶端移行;终蕾上皮细胞游离面可见短小的微绒毛;线粒体、粗面内质网及核糖体随着胎龄增加而逐渐增多,它们均位于细胞顶部.小管期(13~14周) 以呼吸部发育为主,原始肺泡开始形成,呼吸性细支气管衬以未分化的立方上皮;终蕾腺状结构逐渐消失,终蕾上皮细胞由高矮不等的单层柱状上皮逐渐演变为立方形的原始肺泡上皮;细胞游离面可见较多的微绒毛,胞质内线粒体、粗面内质网及核糖体较发达.囊状期(第15周) 呼吸部发育显著,肺内细支气管及其末端呈现出'充气'状态;部分原始肺泡上皮细胞分化为扁平的肺泡Ⅰ型细胞和立方形的肺泡Ⅱ型细胞;Ⅱ型细胞内出现嗜锇小体.肺泡期(16~22周) 以肺泡的形成和分化为主,更多的肺泡上皮分化为扁平的肺泡Ⅰ型细胞和立方形的肺泡Ⅱ型细胞.此期,毛细血管内皮与部分肺泡上皮贴近,可将肺泡上皮细胞区分为3种:Ⅰ型细胞,呈矮柱状或椭圆形,胞质中有较明显的核糖体、扩张内质网及变性线粒体;形成了由Ⅰ型细胞-基膜-内皮细胞组成的气血屏障.Ⅱ型细胞,胞质内含丰富的嗜锇板层小体和核糖体,内质网扩张呈大小不一的泡状,多泡体出现,线粒体膨大变性,细胞游离面可见少数微绒毛.Ⅲ型细胞,为未分化细胞,呈立方形,胞体较小,胞核相对较大,呈圆或椭圆形,胞质少,呈带状,电子密度低,细胞器少. 展开更多
关键词 羊胎 上皮细胞 上皮 内质网 线粒体 核糖体 胎儿 胞质 山羊 周龄
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Notch2、Notch4在大鼠早期肺发育过程中的动态表达 被引量:9
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作者 刘春梅 常立文 +2 位作者 李文斌 汪鸿 张谦慎 《实用儿科临床杂志》 CAS CSCD 北大核心 2006年第2期71-73,共3页
目的探讨Notch2、Notch4在SD大鼠肺发育过程中的作用。方法取孕18、202、1 d胎鼠和孕21 d早产鼠(足月为22 d)生后1、7、14、21 d肺组织标本(n=8),HE染色,进行组织形态学观察、免疫组织化学法和半定量RT-PCR法检测Notch2、Notch4的表达... 目的探讨Notch2、Notch4在SD大鼠肺发育过程中的作用。方法取孕18、202、1 d胎鼠和孕21 d早产鼠(足月为22 d)生后1、7、14、21 d肺组织标本(n=8),HE染色,进行组织形态学观察、免疫组织化学法和半定量RT-PCR法检测Notch2、Notch4的表达。结果孕龄18 d的胎肺见支气管树分支形成,间质中毛细血管毗邻呼吸道分布;20 d至早产生后1 d时,肺组织基本结构为终末囊泡,上皮分隔进一步发展,间质减少,毗邻上皮的毛细血管增多;7、14 d肺泡数目增多并渐成熟,气血屏障变薄;21 d肺泡壁进一步变薄,间质细胞少见。免疫组织化学结果显示,Notch2在孕182、0 d即有表达,21 d表达最强,出生后表达逐渐减弱;Notch4在孕18 d表达最明显,以后呈减弱趋势。RT-PCR检测Notch2、Notch4 mRNA变化与多肽变化规律相似。结论Notch2、Notch4参与大鼠早期肺发育过程。 展开更多
关键词 NOTCH2 NOTCH4 早期肺发育 肺血管发育 胎肺
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有无母体合并症及并发症的胎儿生长受限临床对比分析 被引量:14
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作者 陈梦捷 张晓红 +2 位作者 黄振宇 刘国莉 王山米 《实用妇产科杂志》 CAS CSCD 北大核心 2013年第2期115-119,共5页
目的:探讨有、无合并症及并发症的胎儿生长受限(FGR)孕妇的临床特点及围生儿结局。方法:回顾性分析2009年4月至2011年4月北京大学人民医院产科收治的38例非胎儿畸形的FGR患者的临床资料并随访。其中母体有合并症及并发症25例[FGR(A)组]... 目的:探讨有、无合并症及并发症的胎儿生长受限(FGR)孕妇的临床特点及围生儿结局。方法:回顾性分析2009年4月至2011年4月北京大学人民医院产科收治的38例非胎儿畸形的FGR患者的临床资料并随访。其中母体有合并症及并发症25例[FGR(A)组],母体无合并症及并发症13例[FGR(B)组]。对两组孕妇一般情况、孕期病史、超声检查情况及分娩结局等进行比较分析。结果:①FGR(A)组合并症及并发症中妊娠期高血压疾病占首位(72.0%,18/25)。②两组孕妇仅孕前体重指数(BMI)、胎儿脐动脉血流S/D值异常比例及剖宫产率之间的比较,差异有统计学意义(P<0.05),而孕期增重、产前BMI值和终止妊娠孕周等指标的比较,差异均无统计学意义(P>0.05)。③FGR(A)组中16例孕期采用宫内辅助治疗,FGR(B)组中5例采用宫内辅助治疗。④两组总胎儿丢失率为10.5%(4/38),FGR(A)组丢失胎儿3例,其母体均合并妊娠期高血压疾病;FGR(B)组因脐带扭转胎死宫内终止妊娠1例。⑤FGR(A)组活产新生儿出生体重2051.82±359.43g低于FGR(B)组2385.42±446.03g(P=0.024);两组新生儿平均随访至出生后15.08月,均尚未观察到脑瘫、视网膜病变等并发症发生。结论:非胎儿畸形的FGR的母体合并症及并发症中以妊娠期高血压疾病为常见原因,其可能导致胎儿脐动脉血流S/D异常、手术产发生率升高及新生儿低出生体重等,及时、恰当地进行宫内辅助治疗,与无合并症及并发症FGR孕妇一样获得良好的妊娠结局。 展开更多
关键词 胎儿生长受限 妊娠并发症 生长发育
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