Tapered optical fiber DNA biosensor for detecting Leptospira DNA

Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene.Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 μm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum.Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/μL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach.Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis.

Temperature sensor based on a single-mode tapered optical fiber

A temperature sensor is demonstrated and fabricated by coating the single-mode tapered optical fiber with temperature-sensitive silicone rubber. It works on the change of the evanescent fields in the tapered optical fiber. Small changes in the refractive index of coating film greatly influence the power of evanescent fields, which modulate the transmission optical power in the waist region. The range of temperature measured is from -20℃ to 70 ℃. The results show that the temperature sensor has high temperature sensitivity （0.012 mW/℃） and good repeatability.

Experimental measurement and numerical analysis of fused taper shape for optical fiber coupler

To find out the effect of the shape of fused taper region on the optical fiber coupler, the fiber couplers were fabricated at different drawing speeds with a six-axes fiber coupler machine. The results, which were obtained from the shape of fused taper region measured with microscope, show that there is a close correlation between the cone angle and optical performance of fiber coupler. High-performance fiber coupler cannot be obtained until rheological shape is controlled accurately. The numerical analysis model, which was built based on generalized Maxwell viscoelastic theory, is resolved with ANSYS software. The calculated results accord with the experimental data. It can apply a theoretic basis for forecasting the shape of fiber coupler fabricated under the conditions of different technological parameters.

Relationship between rheological manufacturing process and optical performance of optical fiber coupler

Through theoretical analysis and experiments, the viscoelastic mechanical model of optical fiber coupler in the process of fused biconical taper was established, and the numerical analysis in non-uniform temperature field was made. The results show that the rheological parameters, such as drawing speed and fused temperature, have a tremendous influence on stress distribution and performance of optical fiber coupler, especially the influence of fused temperature. The change of fused temperature by 5℃ can lead to the change of the maximum stress by 30% and stress difference by 20% in the same cross section. The change of temperature gradient by 3% can result in the change of stress difference by 90%. In the present condition of rheological technology, rheological defects such as crystallizations and microcracks are easy to generate in the optical fiber coupler.

Effect of technological parameters on optical performance of fiber coupler

To find out the influence of technological parameters on optical performance of fused optical fiber device, the fiber coupler was served as subject investigated by using the fused biconical taper machining as experimental setup. Fused fiber coupler's optical performances such as insertion loss, excess loss, directivity and uniformity were tested with the optical test system that was constituted of tunable laser and optical spectrum analyzer. Especially the relationship between optical performance and drawing speed was investigated. The experimental results show that the optical performance is closely related to process conditions. At fused temperature of 1 200 ℃, there exists a drawing speed of 150 μms, which makes the device's performance optimum. Out of this speed region, the optical performance drops quickly. At drawing speed of 200 μms, the excess loss is relatively small when the fused temperature is above 1 200 ℃. So the technological parameters have close relationship with optical performance of the coupler, and the good performance coupler can't get until the drawing speed and fused temperature match accurately.

Structure analysis of optical fiber coupler with infrared spectrometry

To obtain excellent performance optical fiber couplers, the structural difference of SiO2 in couplers with different manufacturing techniques was investigated. With 740-FT-IR infrared spectrometric analyzer, the infrared absorption spectrum of SiO2 in couplers at different drawing velocities was measured, and two characteristic peaks in the wavenumber range of 6502000 cm-1 were observed. One characteristic peak is at about 943 cm-1, which is （attributed） to Si—O—Si bond asymmetric stretching vibration, the other is at about 773 cm-1, which is attributed to （Si—O—Si） bond symmetric stretching vibration. From the infrared spectrum, it is found that the intensity and wavenumber of the characteristic peaks are related to the manufacturing technique of couplers. The characteristic peak at （about） 943 cm-1 becomes steeper when increasing the drawing velocity. At the drawing velocity of 150 μm/s, the distance between the two characteristic peaks is maximum, and then the optical fiber coupler has excellent performance, indicating that the performance of the optical fiber coupler has a close relationship with the wavenumber of the two characteristic peaks.

Frequency-stabilized Yb：fiber comb with a tapered single-mode fiber

We demonstrate a stable Yb：fiber frequency comb with supercontinuum generation by using a specially designed tapered single-mode fiber, in which a spectrum spanning from 500 nm to 1500 nm is produced. The carrier-envelope offset signal of the Yb：fiber comb is measured with a signal-to-noise ratio of more than 40 dB and a linewidth narrower than 120 kHz. The repetition rate and carrier-envelope offset signals are simultaneously phase locked to a microwave reference frequency.

直耦增强型CMOS相机技术研究

直耦增强型CMOS(intensified complementary metal-oxide-semiconductor,ICMOS)相机通过将像增强器输出窗与CMOS直接耦合而成,具备灵敏度高、响应速度快、光谱范围可调等特点。本文根据ICMOS的组成结构,分析了阴极、微通道板、荧光屏、CMOS等各组成部件对直耦ICMOS成像性能的影响,提出ICMOS用像增强器、CMOS成像器件的选型依据,并结合公司对像增强器的加工优势,基于18 mm NVT-7像增强器和1英寸CMOS开展了实际微光成像验证。结果表明,采用直耦方式的ICMOS相机能够在5×10^(-4)lx条件下清晰成像,分辨力达到16 lp/mm。此外,用于ICMOS耦合的像增强器增益不宜过高,4000 cd/(m^(2)·lx)比较适宜,另外,在增益适宜的条件下,荧光屏输出亮度对其性能影响不大。

Realization of Tapered Waveguide by Stretching the Rod Waveguide

By stretching the rod waveguide with different velocities in opposite directions,the tapered waveguide can be fabricated.In condition of taking no account of volume expansion caused by heating and under the assumptions of volume conservation,the rod waveguide can be stretched freely in the heated region without being stretched outside of the heated region. A model,which shows the relation of the transition shape and the two factors,that is the ratio of two velocity and the heated region length,is presented for the shape of the taper transition through mathematic deduction.Based on this model,a desired tapered waveguide can be fabricated.The tapered waveguide are widely used for fabricating tapered fiber couplers and sensors.In addition,the conclusion can be used for fabricating fused fiber coupler.

一种涂覆水凝胶的模间干涉型光纤pH传感器

为了实现pH值测量的微型化和智能化,提出了一种涂覆水凝胶的模间干涉型光纤pH传感器,满足了对光纤pH传感器结构紧凑、灵敏度高、稳定性好的需求。模间干涉型光纤干涉仪采用标准单模光纤错位熔接制得,对干涉仪进行拉锥提高其对外界折射率的响应灵敏度。将水凝胶涂覆在拉锥后的光纤干涉仪表面,当传感器周围pH值变化时,会引起感应区折射率的变化进而引起传输光谱发生变化,通过测量干涉仪光谱的漂移即可得到对应溶液的pH值。实验结果表明,传感器拉锥8 000μm后在pH值为1.88~7.61的测量灵敏度为0.76 nm/pH,线性相关系数为0.994。所提出的传感器pH值测量范围广、灵敏度高且成本较低,在pH值检测中具有广阔的应用前景。

拉锥空心光纤的温度与应力传感特性研究

该文设计并制作了一款简易的拉锥空心光纤的高灵敏度传感器,通过实验测量了其温度与应力特性。首先利用光纤熔接机在两段标准单模光纤之间熔接一段空心光纤,再对准空心光纤中间位置进行放电,同时运用两个步进电机在单模光纤两端施加一定的拉力,这导致法布里-珀罗干涉仪转换为马赫-曾德尔干涉仪。实验结果表明,此拉锥过程使光纤传感器的温度灵敏度从理论值0.85 pm/℃提升到69.1 pm/℃,约提升了81.3倍,轴向应变灵敏度最高可达3.6 pm/με。该器件具有体积小、结构简单和灵敏度高等特点,在航空航天、医疗监测等领域具有广阔的应用前景。

Graphene-and Multi-Walled Carbon Nanotubes-Coated Tapered Plastic Optical Fiber for Detection of Lard Adulteration in Olive Oil

A rapid and high-performance sensor for lard adulterant in edible oil was developed using the tapered plastic optical fiber(POF)coated with graphene and multi-walled carbon nanotubes.The coating material was deposited onto a tapered POF with a taper waist diameter and a taper length of 0.45 mm and 1 cm,respectively.The addition of the coating material was used to increase the sensitivity and selectivity coefficient of the tapered POF toward the lard substance.The sensing mechanism is based on a simultaneous interaction of lard substance and an evanescent wave of tapered POF with the coating layers.The results showed that graphene coating on the tapered POF increased the selectivity coefficient of the tapered POF towards lard substance from 33.54 to 324.19,and it gave a sensitivity of 0.427 dBm/%.In comparison,multi-walled carbon nanotubes coating increased the selectivity coefficient to 71.65 and increased its sensitivity to 1.189 dBm/%.Thus,the proposed configuration of the tapered POF with the coating material offered a simple configuration for a rapid,high sensitivity and selectivity detection of lard adulterant in edible oils.

Evanescent Wave Absorption Sensor Based Tapered Plastic Optical Fiber Coated with Monolayer Graphene for Ethanol Molecules Detection

An evanescent wave absorption （EWA） sensor based tapered plastic optical fiber （TPOF） coated with monolayer graphene film for ethanol molecules detection is demonstrated in this study. The continuous and monolayer graphene films were grown on a Cu foil by using chemical vapor deposition （CVD） technology. Polymethyl methacrylate （PMMA） was used as the carrier to support the transfer of graphene from Cu foil to the skinless tapered optical fiber core. The accuracy of the TPOF sensor with graphene （G-TPOF sensor） is much higher than that without gra- phene （TPOF sensor）, which can be attributed to the molecules enriched on the surface of graphene. The absorbance （A） and the concentrations of ethanol solution show an excellent proportional relationship in a range of 0--100%. The dynamic response of the G-TPOF sensor has shown strong reversibility, repeatability and stability at room temperature. The response time and recovery time of the G-TPOF sensor for different concentrations are all less than 30 s. Beyond that, we selected the Chinese liquor as the analyte, and the results are consistent with the concen- tration-list obtained in the experiment.

Nonadiabatic Tapered Optical Fiber for Biosensor Applications

biconical 上的简短评论逐渐变细为 biosensing 应用的纤维传感器被介绍。这个传感器的许多配置和格式为免费 biosensing 基于测量小折射索引改变的标签被设计了。biconical nonadiabatic 逐渐变细光纤维为光察觉到提供很多个有利性质，它在几 biosensing 应用被利用了，包括房间，蛋白质，和 DNA 传感器。这些传感器的类型介绍展示一根缩小的察觉到探针，没有标签的直接察觉，和高敏感的一个便宜纤维生物传感器。

基于CO_(2)激光器的长周期光栅制作及实验

长周期光纤光栅(LPFG)作为一种重要的无源光器件,以其独特的性能和优势被广泛应用于光纤通信与传感领域;近年来发表了许多不同的LPFG制造方法,但产品可靠性依旧是需要解决的关键问题;提出用CO_(2)激光熔融拉伸法制备长周期光纤光栅,改善了在LPFG制备中各种因素影响导致刻写失败的问题,该方法采用CO_(2)激光器对光纤进行加热软化,在步进电机的恒定拉力下拉锥,重复在据上一锥形中心一个光栅周期的位置处加热拉伸,得到在波长为1 539 nm处出现最大衰减峰,峰值大于15 dB的长周期光纤光栅,提高了LPFG的制备成功率及一致性;并对产品的可靠性进行验证,测试其温度、轴向应力的敏感特性;该研究在光纤激光系统及其应用的光学器件方面有较大应用潜力。

基于拉锥细芯光纤的双参量同时测量传感器

该文研制了一种基于拉锥细芯光纤、可同时测量折射率与应变的传感器。传感器结构为“单模光纤-多模光纤-锥形细芯光纤-多模光纤-单模光纤”,由于细芯光纤中部腰锥尺寸变小,构成马赫-曾德尔干涉仪。实验结果表明,传感器透射谱线谐振峰或谐振谷的中心波长与环境折射率和轴向应变之间均有良好的线性关系,折射率灵敏度最高可达-38.677 nm/RIU,轴向应变灵敏度最高为-4.4 pm/με,取两个谐振峰或谐振谷构建矩阵,消除两物理量之间的交叉敏感,能够实现双参量同时测量。该传感器制作简单,成本低,鲁棒性优良,在折射率与轴向应变传感领域有一定的应用价值。

光锥与CCD耦合效率的理论分析

光锥与CCD的耦合技术是研制ICCD图像传感器的关键技术 ,本文主要从理论上分析并讨论了光锥与CCD耦合器件的耦合效率及其影响因素 ,进一步分析讨论了耦合效率对耦合器件信噪比的影响 证明了光锥与CCD的耦合效率不仅降低ICCD探测效率而且降低ICCD信噪比 。

纤维光锥有效透过率的理论分析

光锥的有效透过率不仅影响光锥与CCD的耦合效率,而且会影响到耦合器件的信噪比 本文主要从理论上讨论了影响光锥有效透过率性能的因素,从纤维光学元件的实际内部结构推导出了其实际有效填充率,从光线在光锥中的传输特征推导出了光线在光锥中传播时产生的衰减损耗。

锥形光纤中光波传输特性的蒙特卡罗模拟

为了分析锥形光纤中光子流的传输特性和空间分布特性,采用了蒙特卡罗算法对其进行了模拟仿真,得到了大量光子经过锥形光纤后的光子分布图·模拟实验结果表明,通过锥形光纤的大量光子集中在探测器中心,到达探测器某一位置的光子数随着该位置到探测器中心距离的增加而减少,并且在锥形光纤锥度比一定的条件下,改变锥形光纤长度,光强度随着长度的增加而下降,但通过锥形光纤的光子分布是一样的·这些研究结果对锥形光纤的设计和实际应用提供了重要依据·

光纤耦合CCD相机的平场校正方法研究

对于光纤锥耦合的CCD相机中存在的响应非均性问题进行了阐述 ,针对这种类型的系统的平场校正方法进行了仔细的研究 ,分析了CCD相机输出信号的构成情况 ,并利用这些方法对实际存在较严重的非均匀问题的图像进行处理 ,获得了很好的结果 。