Microneedles(MNs)with unique three-dimensional stereochemical structures are suitable candidates for tissue fixation and drug delivery.However,existing hydrogel MNs exhibit poor mechanical properties after swelling an...Microneedles(MNs)with unique three-dimensional stereochemical structures are suitable candidates for tissue fixation and drug delivery.However,existing hydrogel MNs exhibit poor mechanical properties after swelling and require complex preparation procedures,impeding their practical application.Hence,we engineered chitosan fiber-reinforced silk fibroin MN patches containing epigallocatechin gallate(SCEMN).A formic acid-calcium chloride system was introduced to fabricate hydrogel MNs with excellent inherent adhesion,and the incorporation of chitosan fiber as a reinforcing material enhanced mechanical strength and viscosity,thereby increasing the physical interlocking with tissue and the ability to maintain shape.The SCEMN with a lower insertion force firmly adhered to porcine skin,with a maximum detachment force of 11.98 N/cm2.Additionally,SCEMN has excellent antioxidant and antibacterial properties,facilitates macrophage polarization from M1 to M2,and demonstrates superior performance in vivo for diabetic wound repair compared with the commercial product Tegaderm^(TM).This study represents the first trial of fiber-reinforced hydrogel MNs for robust tissue adhesion.Our findings underscore the significance of this innovative approach for advancing MN technology to enhance tissue adhesion and accel-erate wound healing.展开更多
基金supported by the National Natural Science Foundation of China(No.51803171)the Fundamental Research Funds for Central Universities(No.SWU-XDPY22010).
文摘Microneedles(MNs)with unique three-dimensional stereochemical structures are suitable candidates for tissue fixation and drug delivery.However,existing hydrogel MNs exhibit poor mechanical properties after swelling and require complex preparation procedures,impeding their practical application.Hence,we engineered chitosan fiber-reinforced silk fibroin MN patches containing epigallocatechin gallate(SCEMN).A formic acid-calcium chloride system was introduced to fabricate hydrogel MNs with excellent inherent adhesion,and the incorporation of chitosan fiber as a reinforcing material enhanced mechanical strength and viscosity,thereby increasing the physical interlocking with tissue and the ability to maintain shape.The SCEMN with a lower insertion force firmly adhered to porcine skin,with a maximum detachment force of 11.98 N/cm2.Additionally,SCEMN has excellent antioxidant and antibacterial properties,facilitates macrophage polarization from M1 to M2,and demonstrates superior performance in vivo for diabetic wound repair compared with the commercial product Tegaderm^(TM).This study represents the first trial of fiber-reinforced hydrogel MNs for robust tissue adhesion.Our findings underscore the significance of this innovative approach for advancing MN technology to enhance tissue adhesion and accel-erate wound healing.
