A RAPD marker specific to leaf shape of fig(Ficus carica)was developed and converted into SCAR marker for germplasm identification.RAPD amplifications with random primers S_ 2082 produced a unique fragment linking to ...A RAPD marker specific to leaf shape of fig(Ficus carica)was developed and converted into SCAR marker for germplasm identification.RAPD amplifications with random primers S_ 2082 produced a unique fragment linking to the non-cordate base leaf shape.The unique fragment was extracted,cloned and sequenced.Sequencing results indicated that the size of the fragment was 1 640 bp.Specific primers were designed based on its sequence and used in SCAR-PCR,and it was showed that the RAPD marker from S_ 2082-1640 was successfully converted into SCAR marker.展开更多
利用氯化胆碱和丙三醇(物质的量比1:2)形成的低共熔溶剂做溶剂,提取无花果叶中的黄酮组分。用Al(NO_(3))_(3)-NaNO_(2)-NaOH显色法确定黄酮的含量。单因素试验研究发现,用该低共熔溶剂提取无花果叶黄酮的最佳条件为:在9 mL DES-30%H_(2)...利用氯化胆碱和丙三醇(物质的量比1:2)形成的低共熔溶剂做溶剂,提取无花果叶中的黄酮组分。用Al(NO_(3))_(3)-NaNO_(2)-NaOH显色法确定黄酮的含量。单因素试验研究发现,用该低共熔溶剂提取无花果叶黄酮的最佳条件为:在9 mL DES-30%H_(2)O中,加入0.75 g无花果叶干粉末,在60℃提取1.5 h,用吸光光度仪在510 nm测定黄酮的总含量,提取率为27.9 mg/g。展开更多
文摘A RAPD marker specific to leaf shape of fig(Ficus carica)was developed and converted into SCAR marker for germplasm identification.RAPD amplifications with random primers S_ 2082 produced a unique fragment linking to the non-cordate base leaf shape.The unique fragment was extracted,cloned and sequenced.Sequencing results indicated that the size of the fragment was 1 640 bp.Specific primers were designed based on its sequence and used in SCAR-PCR,and it was showed that the RAPD marker from S_ 2082-1640 was successfully converted into SCAR marker.