Objective To evaluate the antagonistic effects of N-acetylcysteine(NAC)on mitogen-activated protein kinases(MAPK)pathway activation,oxidative stress and inflammatory responses in rats with lung injury induced by fine ...Objective To evaluate the antagonistic effects of N-acetylcysteine(NAC)on mitogen-activated protein kinases(MAPK)pathway activation,oxidative stress and inflammatory responses in rats with lung injury induced by fine particulate matter(PM2.5).Methods Forty eight male Wistar rats were randomly divided into six groups:blank control group(C1),water drip control group(C2),PM2.5 exposed group(P),low-dose NAC treated and PM2.5 exposed group(L),middle-dose NAC treated and PM2.5 exposed group(M),and high-dose NAC treated and PM2.5 exposed group(H).PM2.5 suspension(7.5 mg/kg)was administered tracheally once a week for four times.NAC of 125 mg/kg,250 mg/kg and 500 mg/kg was delivered intragastrically to L,M and H group respectively by gavage(10 ml/kg)for six days before PM2.5 exposure.The histopathological changes and human mucin 5 subtype AC(MUC5AC)content in lung tissue of rats were evaluated.We investigated IL-6 in serum and bronchoalveolar lavage fluid(BALF)by Enzyme-linked immunosorbent assay(ELISA),MUC5AC in lung tissue homogenate by ELISA,glutathione peroxidase(GSH-PX)in serum and BALF by spectrophotometry,and the expression of p-ERK1/2,p-JNK1/2 and p-p38 proteins by Western blot.All the measurements were analyzed and compared statistically.Results Lung tissue of rats exposed to PM2.5 showed histological destruction and increased mucus secretion of bronchial epithelial cells.Rats receiving NAC treatment showed less histological destruction and mucus secretion.Of P,L,M and H group,MUC5AC in lung tissue,IL-6 in serum and BALF were higher than controls(C1 and C2)(all P<0.05),with the highest levels found in the P group and a decreasing trend with increase of NAC dose.The activity of GSH-PX in serum and BALF of PM2.5 exposed rats(P,L,M and H)was lower than that of controls(all P<0.05),with higher activities found in NAC treated rats(L,M,and H),and an increasing trend with increase of NAC dose.The expressions of p-ERK1/2,p-JNK1/2 and p-p38 proteins in PM2.5 exposed lung tissue(P,L,M and H)was higher than controls(all P<0.05),with decreased levels and dose dependent downregulation found in NAC treated rats.Conclusion NAC can antagonize major MAPK pathway activation,lung oxidative stress and inflammatory injury induced by PM2.5 in rats.展开更多
Since 1991, air pollution has gained special attention in Taiwan after a petrochemical complex was constructed in Mailiao Township, Yunlin County. We explored the association between the magnitude of PM2.5 and meteoro...Since 1991, air pollution has gained special attention in Taiwan after a petrochemical complex was constructed in Mailiao Township, Yunlin County. We explored the association between the magnitude of PM2.5 and meteorological factors during 2012-2016. Our findings revealed that 1) mean PM2.5 levels gradually decreased from 30.70 μg/m3 in 2013 to 25.36 μg/m3 in 2016;2) wind speed is the main determinant of air quality—air quality significantly improved when it was faster than 4 m/sec;and 3) wind direction is another determinant of air quality—when the wind direction was southerly, air quality improved. Elevated PM2.5 levels were defined as those hourly levels higher than the third quartile (36 μg/m3). The significantly negative predictive factors for elevated PM2.5 levels were the summer or autumn seasons, rainfall, increased wind speed, and wind direction from 150° to 230° from the north. The significantly positive predictive factors for elevated PM2.5 levels were working hours from 6 a.m. to 2 p.m., a temperature between 11°C and 25°C, relative humidity between 40% and 68%, and wind direction (e.g., northerly wind, northeasterly wind, and easterly wind). The predictive formula is attached in the Appendix. Therefore, people should protect themselves on these high-risk days.展开更多
To assess the exposure of residents in rural communities in the Yukon Flats to particulate matter of 2.5 μm or less in diameter (PM<sub><span style="font-family:Verdana;">2.5</span></su...To assess the exposure of residents in rural communities in the Yukon Flats to particulate matter of 2.5 μm or less in diameter (PM<sub><span style="font-family:Verdana;">2.5</span></sub><span style="font-family:Verdana;">), both indoor and outdoor concentration observations were carried out from March to September 2019 in Ft. Yukon, Alaska. Indoor concentrations were measured at 0.61 m (breathing level during sleeping) in homes and at 1.52 m heights (breathing level of standing adult) in homes and office/commercial buildings. Air quality was better at both heights in cabins than frame homes both during times with and without surface-based inversions. In frame houses, concentrations were higher at 0.61 m than 1.52 m, while the opposite is true typically for cabins. Differences between shoulder season and summer indoor concentrations in residences were related to changes in heating, subsistence lifestyle and mosquito repellents. In summer, office and commercial buildings, air quality decreased due to increased indoor emissions related to increased use of equipment and mosquito pics as well as more merchandise. During summer indoor concentrations reached unhealthy for sensitive groups to hazardous conditions for extended times that even exceeded the high outdoor concentrations. Due to nearby wildfires, July mean outdoor concentrations were 55.3 μg·m<sup>-</sup></span><sup><span style="font-family:Verdana;">3</span></sup><span style="font-family:Verdana;"> which exceeds the 24-h US National Ambient Air Quality Standard of 35 μg·m</span><span style="font-family:Verdana;"><sup>-3</sup></span><span style="font-family:Verdana;">. Indoor and outdoor concentrations correlated the strongest with each other for office/commercial buildings, followed by frame houses and cabins. Office/commercial buildings with temperature monitors had one to two orders of magnitude lower concentrations than those without.</span>展开更多
Background: Despite the adverse effects of ambient fine particulate matter (PM_(2.5) ) on type 2 diabetes and the beneficial role of physical activity (PA), the influence of PM_(2.5) on the relationship between PA and...Background: Despite the adverse effects of ambient fine particulate matter (PM_(2.5) ) on type 2 diabetes and the beneficial role of physical activity (PA), the influence of PM_(2.5) on the relationship between PA and type 2 diabetes remains unclear.Methods: In this prospective study with 71,689 participants, PA was assessed by a questionnaire and was categorized into quartiles for volume and three groups for intensity. Long-term PM_(2.5) exposure was calculated using 1-km resolution satellite-based PM_(2.5) estimates. PM_(2.5) exposure and PA's effect on type 2 diabetes were assessed by cohort-stratified Cox proportional hazards models, individually and in combination.Results: In 488,166 person-years of follow-up, 5487 incident type 2 diabetes cases were observed. The association between PA and type 2 diabetes was modified by PM_(2.5) . Compared with the lowest quartile of PA volume, the highest quartile was associated with reduced type 2 diabetes risk in low PM_(2.5) stratification (≤65.02 μg/m^(3)) other than in high PM_(2.5) stratification (>65.02 μg/m^(3)), with the hazard ratio (HR) of 0.75 (95% confidence interval [CI]: 0.66-0.85) and 1.10 (95% CI: 0.99-1.22), respectively. Similar results were observed for PA intensity. High PM_(2.5) exposure combined with the highest PA levels increased the risk of type 2 diabetes the most (HR= 1.79, 95% CI: 1.59-2.01 for PA volume;HR = 1.82, 95% CI: 1.64-2.02 for PA intensity).Conclusion: PA could reduce type 2 diabetes risk in low-pollution areas, but high PM_(2.5) exposure may weaken or even reverse the protective effects of PA. Safety and health benefits of PA should be thoroughly assessed for long-term polluted residents.展开更多
Air pollution has been the most important health issue in recent years. In this study, the aim was to evaluate the results of regular measurements of air pollutants PM10 （Particulate Matter of 10 Microns in Diameter...Air pollution has been the most important health issue in recent years. In this study, the aim was to evaluate the results of regular measurements of air pollutants PM10 （Particulate Matter of 10 Microns in Diameter） and SO2 （Sulfur Dioxide） concentrations in the city of Istanbul by taking the years 2013 and 2015 as a sample. The data were obtained through the website http：//www.havaizleme.gov.tr, which was published by the Administration of Marmara Clean Air Center of Ministry of Environment and Urbanization in Turkey. For the years 2013 and 2015, the mean SO2 concentration was 8.35 ± 6.04 and 10.60 ±7.16 μg/m^3. The mean PMI0 concentration was 73.06 ± 30.63 μg/m^3 for 2013 and 51.57 ± 18.84μg/m^3 for 2015. The acceptable upper limit values by WHO （World Health Organization） for daily mean SO2 and PMI0 concentrations respectively are 20 μg/m^3 and 50μg/m^3. In Istanbul, SO2 concentrations were above the upper limit values recommended by WHO, but PM10 concentrations during 2013 and 2015 were over the recommended limit values by WHO. As the particulate matter pollution is at high concentrations during these two years, it has shown that air pollution emerges as a problem awaiting solutions in Istanbul, where is industrially intense, highly populated and also with high traffic density.展开更多
文摘Objective To evaluate the antagonistic effects of N-acetylcysteine(NAC)on mitogen-activated protein kinases(MAPK)pathway activation,oxidative stress and inflammatory responses in rats with lung injury induced by fine particulate matter(PM2.5).Methods Forty eight male Wistar rats were randomly divided into six groups:blank control group(C1),water drip control group(C2),PM2.5 exposed group(P),low-dose NAC treated and PM2.5 exposed group(L),middle-dose NAC treated and PM2.5 exposed group(M),and high-dose NAC treated and PM2.5 exposed group(H).PM2.5 suspension(7.5 mg/kg)was administered tracheally once a week for four times.NAC of 125 mg/kg,250 mg/kg and 500 mg/kg was delivered intragastrically to L,M and H group respectively by gavage(10 ml/kg)for six days before PM2.5 exposure.The histopathological changes and human mucin 5 subtype AC(MUC5AC)content in lung tissue of rats were evaluated.We investigated IL-6 in serum and bronchoalveolar lavage fluid(BALF)by Enzyme-linked immunosorbent assay(ELISA),MUC5AC in lung tissue homogenate by ELISA,glutathione peroxidase(GSH-PX)in serum and BALF by spectrophotometry,and the expression of p-ERK1/2,p-JNK1/2 and p-p38 proteins by Western blot.All the measurements were analyzed and compared statistically.Results Lung tissue of rats exposed to PM2.5 showed histological destruction and increased mucus secretion of bronchial epithelial cells.Rats receiving NAC treatment showed less histological destruction and mucus secretion.Of P,L,M and H group,MUC5AC in lung tissue,IL-6 in serum and BALF were higher than controls(C1 and C2)(all P<0.05),with the highest levels found in the P group and a decreasing trend with increase of NAC dose.The activity of GSH-PX in serum and BALF of PM2.5 exposed rats(P,L,M and H)was lower than that of controls(all P<0.05),with higher activities found in NAC treated rats(L,M,and H),and an increasing trend with increase of NAC dose.The expressions of p-ERK1/2,p-JNK1/2 and p-p38 proteins in PM2.5 exposed lung tissue(P,L,M and H)was higher than controls(all P<0.05),with decreased levels and dose dependent downregulation found in NAC treated rats.Conclusion NAC can antagonize major MAPK pathway activation,lung oxidative stress and inflammatory injury induced by PM2.5 in rats.
文摘Since 1991, air pollution has gained special attention in Taiwan after a petrochemical complex was constructed in Mailiao Township, Yunlin County. We explored the association between the magnitude of PM2.5 and meteorological factors during 2012-2016. Our findings revealed that 1) mean PM2.5 levels gradually decreased from 30.70 μg/m3 in 2013 to 25.36 μg/m3 in 2016;2) wind speed is the main determinant of air quality—air quality significantly improved when it was faster than 4 m/sec;and 3) wind direction is another determinant of air quality—when the wind direction was southerly, air quality improved. Elevated PM2.5 levels were defined as those hourly levels higher than the third quartile (36 μg/m3). The significantly negative predictive factors for elevated PM2.5 levels were the summer or autumn seasons, rainfall, increased wind speed, and wind direction from 150° to 230° from the north. The significantly positive predictive factors for elevated PM2.5 levels were working hours from 6 a.m. to 2 p.m., a temperature between 11°C and 25°C, relative humidity between 40% and 68%, and wind direction (e.g., northerly wind, northeasterly wind, and easterly wind). The predictive formula is attached in the Appendix. Therefore, people should protect themselves on these high-risk days.
文摘To assess the exposure of residents in rural communities in the Yukon Flats to particulate matter of 2.5 μm or less in diameter (PM<sub><span style="font-family:Verdana;">2.5</span></sub><span style="font-family:Verdana;">), both indoor and outdoor concentration observations were carried out from March to September 2019 in Ft. Yukon, Alaska. Indoor concentrations were measured at 0.61 m (breathing level during sleeping) in homes and at 1.52 m heights (breathing level of standing adult) in homes and office/commercial buildings. Air quality was better at both heights in cabins than frame homes both during times with and without surface-based inversions. In frame houses, concentrations were higher at 0.61 m than 1.52 m, while the opposite is true typically for cabins. Differences between shoulder season and summer indoor concentrations in residences were related to changes in heating, subsistence lifestyle and mosquito repellents. In summer, office and commercial buildings, air quality decreased due to increased indoor emissions related to increased use of equipment and mosquito pics as well as more merchandise. During summer indoor concentrations reached unhealthy for sensitive groups to hazardous conditions for extended times that even exceeded the high outdoor concentrations. Due to nearby wildfires, July mean outdoor concentrations were 55.3 μg·m<sup>-</sup></span><sup><span style="font-family:Verdana;">3</span></sup><span style="font-family:Verdana;"> which exceeds the 24-h US National Ambient Air Quality Standard of 35 μg·m</span><span style="font-family:Verdana;"><sup>-3</sup></span><span style="font-family:Verdana;">. Indoor and outdoor concentrations correlated the strongest with each other for office/commercial buildings, followed by frame houses and cabins. Office/commercial buildings with temperature monitors had one to two orders of magnitude lower concentrations than those without.</span>
基金National Natural Science Foundation of China(Grant/Award Numbers: 82030102, 82373676)National High Level Hospital Clinical Research Funding(Grant/Award Numbers: 2022-GSP-GG-1, 2022-GSP-GG-2)+2 种基金National Key Research and Development Program of China(Grant/Award Numbers: 2017YFC0211700, 2018YFE0115300)Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(Grant/Award Numbers: 2017-I2M-1-004, 2019-I2M-2-003, 2021-I2M-1-010)Research Unit of Prospective Cohort of Cardiovascular Diseases and Cancers, Chinese Academy of Medical Sciences(Grant/Award Number: 2019RU038)。
文摘Background: Despite the adverse effects of ambient fine particulate matter (PM_(2.5) ) on type 2 diabetes and the beneficial role of physical activity (PA), the influence of PM_(2.5) on the relationship between PA and type 2 diabetes remains unclear.Methods: In this prospective study with 71,689 participants, PA was assessed by a questionnaire and was categorized into quartiles for volume and three groups for intensity. Long-term PM_(2.5) exposure was calculated using 1-km resolution satellite-based PM_(2.5) estimates. PM_(2.5) exposure and PA's effect on type 2 diabetes were assessed by cohort-stratified Cox proportional hazards models, individually and in combination.Results: In 488,166 person-years of follow-up, 5487 incident type 2 diabetes cases were observed. The association between PA and type 2 diabetes was modified by PM_(2.5) . Compared with the lowest quartile of PA volume, the highest quartile was associated with reduced type 2 diabetes risk in low PM_(2.5) stratification (≤65.02 μg/m^(3)) other than in high PM_(2.5) stratification (>65.02 μg/m^(3)), with the hazard ratio (HR) of 0.75 (95% confidence interval [CI]: 0.66-0.85) and 1.10 (95% CI: 0.99-1.22), respectively. Similar results were observed for PA intensity. High PM_(2.5) exposure combined with the highest PA levels increased the risk of type 2 diabetes the most (HR= 1.79, 95% CI: 1.59-2.01 for PA volume;HR = 1.82, 95% CI: 1.64-2.02 for PA intensity).Conclusion: PA could reduce type 2 diabetes risk in low-pollution areas, but high PM_(2.5) exposure may weaken or even reverse the protective effects of PA. Safety and health benefits of PA should be thoroughly assessed for long-term polluted residents.
文摘Air pollution has been the most important health issue in recent years. In this study, the aim was to evaluate the results of regular measurements of air pollutants PM10 （Particulate Matter of 10 Microns in Diameter） and SO2 （Sulfur Dioxide） concentrations in the city of Istanbul by taking the years 2013 and 2015 as a sample. The data were obtained through the website http：//www.havaizleme.gov.tr, which was published by the Administration of Marmara Clean Air Center of Ministry of Environment and Urbanization in Turkey. For the years 2013 and 2015, the mean SO2 concentration was 8.35 ± 6.04 and 10.60 ±7.16 μg/m^3. The mean PMI0 concentration was 73.06 ± 30.63 μg/m^3 for 2013 and 51.57 ± 18.84μg/m^3 for 2015. The acceptable upper limit values by WHO （World Health Organization） for daily mean SO2 and PMI0 concentrations respectively are 20 μg/m^3 and 50μg/m^3. In Istanbul, SO2 concentrations were above the upper limit values recommended by WHO, but PM10 concentrations during 2013 and 2015 were over the recommended limit values by WHO. As the particulate matter pollution is at high concentrations during these two years, it has shown that air pollution emerges as a problem awaiting solutions in Istanbul, where is industrially intense, highly populated and also with high traffic density.
