Estimation-free spatial-domain image reconstruction of structured illumination microscopy

Structured illumination microscopy(SIM)achieves super-resolution(SR)by modulating the high-frequency information of the sample into the passband of the optical system and subsequent image reconstruction.The traditional Wiener-filtering-based reconstruction algorithm operates in the Fourier domain,it requires prior knowledge of the sinusoidal illumination patterns which makes the time-consuming procedure of parameter estimation to raw datasets necessary,besides,the parameter estimation is sensitive to noise or aberration-induced pattern distortion which leads to reconstruction artifacts.Here,we propose a spatial-domain image reconstruction method that does not require parameter estimation but calculates patterns from raw datasets,and a reconstructed image can be obtained just by calculating the spatial covariance of differential calculated patterns and differential filtered datasets(the notch filtering operation is performed to the raw datasets for attenuating and compensating the optical transfer function(OTF)).Experiments on reconstructing raw datasets including nonbiological,biological,and simulated samples demonstrate that our method has SR capability,high reconstruction speed,and high robustness to aberration and noise.

Ultrafast dynamics of femtosecond laser-induced high spatial frequency periodic structures on silicon surfaces

Femtosecond laser-induced periodic surface structures(LIPSS)have been extensively studied over the past few decades.In particular,the period and groove width of high-spatial-frequency LIPSS(HSFL)is much smaller than the diffraction limit,making it a useful method for efficient nanomanufacturing.However,compared with the low-spatial-frequency LIPSS(LSFL),the structure size of the HSFL is smaller,and it is more easily submerged.Therefore,the formation mechanism of HSFL is complex and has always been a research hotspot in this field.In this study,regular LSFL with a period of 760 nm was fabricated in advance on a silicon surface with two-beam interference using an 800 nm,50 fs femtosecond laser.The ultrafast dynamics of HSFL formation on the silicon surface of prefabricated LSFL under single femtosecond laser pulse irradiation were observed and analyzed for the first time using collinear pump-probe imaging method.In general,the evolution of the surface structure undergoes five sequential stages:the LSFL begins to split,becomes uniform HSFL,degenerates into an irregular LSFL,undergoes secondary splitting into a weakly uniform HSFL,and evolves into an irregular LSFL or is submerged.The results indicate that the local enhancement of the submerged nanocavity,or the nanoplasma,in the prefabricated LSFL ridge led to the splitting of the LSFL,and the thermodynamic effect drove the homogenization of the splitting LSFL,which evolved into HSFL.

Drivers of spatial structure in thinned forests

Background:As is widely known,an increasing number of forest areas were managed to preserve and enhance the health of forest ecosystems.However,previous research on forest management has often overlooked the importance of structure-based.Aims:Our objectives were to define the direction of structure-based forest management.Subsequently,we investigated the relationships between forest structure and the regeneration,growth,and mortality of trees under different thinning treatments.Ultimately,the drivers of forest structural change were explored.Methods:On the basis of 92 sites selected from northeastern China,with different recovery time (from 1 to 15years) and different thinning intensities (0–59.9%) since the last thinning.Principal component analysis (PCA)identified relationships among factors determining forest spatial structure.The structural equation model (SEM)was used to analyze the driving factors behind the changes in forest spatial structure after thinning.Results:Light thinning (0–20%trees removed) promoted forest regeneration,and heavy thinning (over 35% of trees removed) facilitated forest growth.However,only moderate thinning (20%–35%trees removed) created a reasonable spatial structure.While dead trees were clustered,and they were hardly affected by thinning intensity.Additionally,thinning intensity,recovery time,and altitude indirectly improve the spatial structure of the forest by influencing diameter at breast height (DBH) and canopy area.Conclusion:Creating larger DBH and canopy area through thinning will promote the formation of complex forest structures,which cultivates healthy and stable forests.

Numerical Simulation of Super-Resolution Structured Illumination Microscopy (SIM) Using Heintzmann-Cremer Algorithm with Non-Continuous Spatial Frequency Support

We report a comprehensive numerical study of super resolution (SR) structured illumination microscopy (SIM) utilizing the classic Heintzmann-Cremer SIM process and algorithm. In particular, we investigated the impact of the diffraction limit of the underlying imaging system on the optimal SIM grating frequency that can be used to obtain the highest SR enhancement with non-continuous spatial frequency support. Besides confirming the previous theoretical and experimental work that SR-SIM can achieve an enhancement close to 3 times the diffraction limit with grating pattern illuminations, we also observe and report a series of more subtle effects of SR-SIM with non-continuous spatial frequency support. Our simulations show that when the SIM grating frequency exceeds twice that of the diffraction limit, the higher SIM grating frequency can help achieve a higher SR enhancement for the underlying imaging systems whose diffraction limit is low, though this enhancement is obtained at the cost of losing resolution at some lower resolution targets. Our simulations also show that, for underlying imaging systems with high diffraction limits, however, SR-SIM grating frequencies above twice the diffraction limits tend to bring no significant extra enhancement. Furthermore, we observed that there exists a limit grating frequency above which the SR enhancement effect is lost, and the reconstructed images essentially have the same resolution as the one obtained directly from the underlying imaging system without using the SIM process.

The Study on the Evolution of Urban Spatial Structure in Zhuhai City Based on Spatial Syntax

With the deepening of the Guangdong-Hong Kong-Macao Greater Bay Area strategy and the accelerated integration and development of the east and west sides of the Pearl River Estuary,Zhuhai’s hub position is becoming more and more prominent.The city of Zhuhai has a dense water network and is divided into two urban areas,the east and the west,under the influence of the Mordor Gate waterway.Based on the theory of spatial syntax,this paper carries out an analytical study on the urban spatial structure of Zhuhai,identifies the distribution characteristics of urban POIs,and provides theoretical support for the urban development of Zhuhai.

A Discussion on the Structure and Spatial Composition of Long Museum West Bund

The Long Museum,with its distinctive T-shaped“umbrella-vault”structure,offers a rich array of spatial experiences.This article delves into space from both structural and compositional perspectives.As the construction mediator,the umbrella vault embodies the consistency of construction and implies the causality of the surface.Presented as an element,the space flows freely,showing diverse fields and order.In terms of the liberation of its meaning,the space offers static perception and dynamic experience,creating a rich exhibition atmosphere.As a distinct entity,the umbrella-vault presents the physical nature,and the dialogue between the Long Museum and the city makes it a museum of art for the people.

Large Eddy Simulation of Vertical Structure and Size Density of Deep Layer Clouds

In a convective scheme featuring a discretized cloud size density, the assumed lateral mixing rate is inversely proportional to the exponential coefficient of plume size. This follows a typical assumption of-1, but it has unveiled inherent uncertainties, especially for deep layer clouds. Addressing this knowledge gap, we conducted comprehensive large eddy simulations and comparative analyses focused on terrestrial regions. Our investigation revealed that cloud formation adheres to the tenets of Bernoulli trials, illustrating power-law scaling that remains consistent regardless of the inherent deep layer cloud attributes existing between cloud size and the number of clouds. This scaling paradigm encompasses liquid, ice, and mixed phases in deep layer clouds. The exponent characterizing the interplay between cloud scale and number in the deep layer cloud, specifically for liquid, ice, or mixed-phase clouds, resembles that of shallow convection,but converges closely to zero. This convergence signifies a propensity for diminished cloud numbers and sizes within deep layer clouds. Notably, the infusion of abundant moisture and the release of latent heat by condensation within the lower atmospheric strata make substantial contributions. However, this role in ice phase formation is limited. The emergence of liquid and ice phases in deep layer clouds is facilitated by the latent heat and influenced by the wind shear inherent in the middle levels. These interrelationships hold potential applications in formulating parameterizations and post-processing model outcomes.

A process monitoring method for autoregressive-dynamic inner total latent structure projection

As a dynamic projection to latent structures(PLS)method with a good output prediction ability,dynamic inner PLS(DiPLS)is widely used in the prediction of key performance indi-cators.However,due to the oblique decomposition of the input space by DiPLS,there are false alarms in the actual industrial process during fault detection.To address the above problems,a dynamic modeling method based on autoregressive-dynamic inner total PLS(AR-DiTPLS)is proposed.The method first uses the regression relation matrix to decompose the input space orthogonally,which reduces useless information for the predic-tion output in the quality-related dynamic subspace.Then,a vector autoregressive model(VAR)is constructed for the predic-tion score to separate dynamic information and static informa-tion.Based on the VAR model,appropriate statistical indicators are further constructed for online monitoring,which reduces the occurrence of false alarms.The effectiveness of the method is verified by a Tennessee-Eastman industrial simulation process and a three-phase flow system.

Spatial structural characteristics of the Deda ancient landslide in the eastern Tibetan Plateau:Insights from Audio-frequency Magnetotellurics and the Microtremor Survey Method

It is of crucial importance to investigate the spatial structures of ancient landslides in the eastern Tibetan Plateau’s alpine canyons as they could provide valuable insights into the evolutionary history of the landslides and indicate the potential for future reactivation.This study examines the Deda ancient landslide,situated in the Chalong-ranbu fault zone,where creep deformation suggests a complex underground structure.By integrating remote sensing,field surveys,Audio-frequency Magnetotellurics(AMT),and Microtremor Survey Method(MSM)techniques,along with engineering geological drilling for validation,to uncover the landslide’s spatial feature s.The research indicates that a fault is developed in the upper part of the Deda ancient landslide,and the gully divides it into Deda landslide accumulation zoneⅠand Deda landslide accumulation zoneⅡin space.The distinctive geological characteristics detectable by MSM in the shallow subsurface and by AMT in deeper layers.The findings include the identification of two sliding zones in the Deda I landslide,the shallow sliding zone(DD-I-S1)depth is approximately 20 m,and the deep sliding zone(DD-I-S2)depth is 36.2-49.9 m.The sliding zone(DD-Ⅱ-S1)depth of the DedaⅡlandslide is 37.6-43.1 m.A novel MSM-based method for sliding zone identification is proposed,achieving less than 5%discrepancy in depth determination when compared with drilling data.These results provide a valuable reference for the spatial structural analysis of large-deepseated landslides in geologically complex regions like the eastern Tibetan Plateau.

A novel model to evaluate spatial structure in thinned conifer-broadleaved mixed natural forests

In order to ensure the effective analysis and reconstruction of forests,it is key to ensure the quantitative description of their spatial structure.In this paper,a distance model for the optimal stand spatial structure based on weighted Voronoi diagrams is proposed.In particular,we provide a novel methodological model for the comprehensive evaluation of the spatial structure of forest stands in natural mixed conifer-broadleaved forests and the formulation of management decision plans.The applicability of the rank evaluation and the optimal solution distance model are compared and assessed for different standard sample plots of natural mixed conifer-broadleaved forests.The effect of crown width on the spatial structure unit of the trees is observed to be higher than that of the diameter at breast height.Moreover,the influence of crown length is greater than that of tree height.There are nine possible spatial structure units determined by the weighted Voronoi diagram for the number of neighboring trees in the central tree,with an average intersection of neighboring crowns reaching 80%.The rank rating of natural forest sample plots is correlated with the optimal solution distance model,and their results are generally consistent for natural forests.However,the rank rating is not able to provide a quantitative assessment.The optimal solution distance model is observed to be more comprehensive than traditional methods for the evaluation of the spatial structure of forest stands.It can effectively reflect the trends in realistic stand spatial structure factors close to or far from the ideal structure point,and accurately assesses the forest spatial structure.The proposed optimal solution distance model improves the integrated evaluation of the spatial structure of forest stands and provides solid theoretical and technical support for sustainable forest management.

Evolution of the Spatial Distribution of Basic Infrastructures and Services in the Niakhar Area (Fatick), Senegal

Basic infrastructures and services present an important socio-economic and territorial equity issue, because the availability of these makes it possible to improve the living conditions of populations, makes territories attractive and stimulates their development. The objective of our study has been achieved, so our results provided information on the rate of propagation of basic infrastructures and services, as well as the evolution of the forms of spatial distribution of the latter. The data used comes from the census and geolocation of basic infrastructures and services in the Niakhar area, carried out by the IRD in 2018. Centrographic measurements were used to describe the overall spatial distribution and the use of chi-square statistics confirmed the existence of a preferential direction of distribution. To verify the existence of a spatial structure of infrastructure and service seeding, the Ripley statistic is used. Our results can be useful for land use planning and spatial resource allocation policies. Indeed, the identification of different types of spatial aggregates and the highlighting of the preferred directions of the distribution of basic infrastructures and services, make it possible to correct disparities in the spatial distribution of basic resources.

Identifying the Spatial Structure of Black Hole and Tropical Cyclone Based on a Theoretical Analysis of Orthogonal Interaction

Black holes are recognized by Newton’s gravitational theory and Einstein’s general relativity, but there is still a lack of understanding the spatial structure of events, especially the nature of event horizon. In this paper, a theoretical analysis is used to compare the structures of tropical cyclone in the atmosphere and black hole in the astronomy so that five results are: 1) Both of them share the similar spatial structure, with tropical cyclone and black hole having the outflow cloud shield and the horizon sphere in the central part, respectively, while four spiral material bands exist in the rotating plane around them;2) In theoretically, the energy density formed by the orthogonal interaction of the four spiral material bands is as times as the total kinetic energy of the head-on interaction;3) This region of high energy density can lead to the conversion from mass to energy and the creation of new physical states of matter, which is a black hole event;4) The outer horizon of a black hole is the outermost interface of events, or the orthogonal interaction interface of particles;5) High-speed plasma jets extended at the poles of the black hole are directly associated with the shear stress of orthogonal interaction.

Fine-scale genetic structure of Eremosparton songoricum and implication for conservation

Eremosparton songoricum (Litv.) Vass. is a clonal shrub that reproduces both asexually by under-ground rhizomes and sexually by seeds. It is now a rare species with a narrow distribution in fragmented habitat patches in the Gurbantunggut Desert of Xinjiang, China. The objective of this study was to determine the spatial pattern or structure of genetic variation within population. The within-populations genetic structure of E. songoricum in a plot (10 m×10 m) was analyzed using inter simple sequence repeats (ISSR) markers. Correlograms of Moran’s I showed significant positive value was about 7 m, but changed into a negative correlation with the increase of distance, indicating strong genetic structure. The natural character of clonal reproduction, restricted seed and pollen dispersal were the main factors of influencing the spatial pattern. The knowledge of clonal structures within populations was crucial for understanding evolutionary processes and ecological adaptation. This study provided basic data for the conservation and management of E. songoricum, especially for sampling strategies for ex situ conservation.

Spatial structure evolution of overlying strata and inducing mechanism of rockburst in coal mine

Spatial structure of overlying strata existed and evolved dynamically with the exploitation scope （boundary conditions） changes in coal mines and to induce rockburst. Based on the“key strata”theory, the integrated spatial structure of overlying strata was put forward, which was composed of “O-X” structure in the plane section and “F” structure in the vertical section. The formation and ongoing instability of the“O-X”and“F”structures were called as dynamic evolution cycle of the overlying strata. Three basic categories of “O-X”, “F” and “T” structures were defined, and the strata behaviors of each spatial structure were analyzed. According to energy theory, mechanism of rockburst induced by spatial structure instability was discussed. The research expanded the scope of traditional ground pressure theory and provided a guide for the prevention of rockburst and mining tremors induced by structure instability of overlying

Phytoplankton Community Structure and Its Spatial Distribution along Anhui-Jiangsu Reaches of the Yangtze River in Autumn 2009

[Objective] Surveys of phytoplankton were taken during the autumn of 2009 along Anhui-Jiangsu reaches of the Yangtze River to understand the species com- position and their spatial distributions. [Method] Phytoplankton species, their quanti- ties, biomass and the Mcnaughton＇s dominance indices were all measured and their spatial distribution characteristics were determined using cluster analysis. [Result] There were 27 species, belonging to 5 phyla, namely Chlorophyta, Bacillariophyta, Cyanophyta, Euglenophyta and Cryptophyta, of phytoplankton collected and identified from the surveys. Results showed that Bacillariophyta was the predominant phyto- plankton with 16 species collected which accounted for 59.3% of the total species identified. The number of species collected belonging to Chlorophyta, Cyanophyta, Cryptophyta and Euglenophyta were 6, 2, 2, and 1, accounting for 22.2%, 7.4%, 7.4% and 3.7% of the total number identified, respectively. At the species level, the predominant species were Fragilaria crotonensis, Melosira italica, Navicula crypto- cephala, Cyclotella striata and Chroomonas acuta. Phytoplankton abundance was ranging from 5.68×10^4 to 7.08×10^4 cells/L with its average of 6.01×10^4 cells/L. Phyto- plankton biomass was ranging from 30.43 to 34.73 μg/L with its average of 32.46 μg/L. Compared with the previous reports, the number of phytoplankton species was decreased but its abundance and biomass was increased along the Jiangsu reach of the Yangtze River. However, Bacillariophyta species were still the predominant species and the phytoplankton community structure had not significantly changed from the previous studies. [Conclusion] These results might be explained as that the water quality in the Yangtze River was deteriorated but had not come to the worst. The results of similarity analysis gave two clusters of phytoplankton community as Nanjing, Wuhu and Jiangyin sampling sites were clustered into one group and Tongling and Anqing were clustered into another group.

Study upon the Spatial Structure of Regional Eco-tourism-Based upon the Perspective of Supply and Demand and Competition Field

Along with the coming of the low-carbon era, people have paid more and more attention to the natural environment and eco-tourism will embrace a huge development. From the perspectives of the market relationship of supply-demand in economics and of field competition in physics, this paper has discussed upon the present status of the spatial structure of eco-tourism, and analyzed the relationship between supply-demand and field, in order to clarify the direction for the balance between supply and demand in the field and to guide eco-tourism to the way of sustainable development.

Study on Spatial Structure of Rural Tourism in Wuhan Urban Agglomerations

Spatial structure of rural tourism in the urban agglomerations of Wuhan was analyzed by comprehensively studying relevant documents about rural tourism,and reviewing previous researches on the concept and spatial structure of rural tourism.Through investigating rural tourist resources in the study area,resource advantages and characteristics of 9 cities were introduced,and the Gini Coefficient was taken to quantitatively analyze spatial patterns of its rural tourist villages,the obviously imbalanced concentration of these villages was pointed out.By measuring connectivity and accessibility of regional spaces in the study area,its traffic connectivity was proved moderate,and accessibility of each tourist village and town was fine.Then an optimized spatial structure was proposed for the rural tourism development in Wuhan Urban Agglomerations,that is,"one core,one belt and three districts".

Study on Spatial Structure and Response of Towns and Villages System——with Dongzhai Town in Ningwu County of Shanxi Province as a Study Case

Taking Dongzhai Town in Ningwu County of Shanxi Province as example, this essay explores the spatial structure of towns and villages system from the following perspectives: scale structure, function structure, structural characteristics of village land-use, and spatial distribution characteristics of Dongzhai villages. There are 5 grades of villages according to their scales, that is, villages have a population over 1 000, between 500 and 1 000, between 100 and 500, less than 100, less than 50. The function structure of Dongzhai Town is divided into 3 grades, Grade 1 (core village), is village with such functions as tourist service, transportation and distribution function. Grade 2 (characteristic village), is agricultural settlement with functions such as industrial function, transportation service, tourist service, and forestry function. Grade 3 (general village), is agricultural settlements with prominent agricultural functions (dry farming). This essay also predicts the change and responses of this town based on the population forecast models. It is predicted that the population of Jiancheng District will reach 4 750 in 2010, 6 400 in 2015, 8 700 in 2020. Returning farmland to forests in Fenhe River valley can ensure the conservation of water resources and the development of tourist service. The proportion of forest land, especially bushes land, and proportion of grassland, especially artificial grass will increase dramatically. The number of slope land will be down a bit. The Lands for settlement and transportation will increase with the development of ecological migration and tourist service. Specialized land such as water area and places of interest will skyrocket. Village patterns and spatial distribution under the system of towns and villages will be improved as well.

Topology Structure Synthesis and Analysis of Spatial Pyramid Deployable Truss Structures for Satellite SAR Antenna

Many attentions for structural synthesis are paid to planar linkages and parallel mechanisms, while design novel pyramid deployable truss structure（PDTS） of satellite SAR mainly depends on experience of designer. To design novel configuration of PDTS, a two-step topology structure synthesis and analysis approach is proposed. Firstly, a conceptual configuration of PDTS is synthesized. Weighted graph and weighted adjacency matrix are established to realize topological description for PDTS. Graph properties are then summarized to distinguish differentia between PDTS and other type structures. According to graph properties, a procedure for synthesis conceptual configuration of PDTS is presented. Secondly, join relationship of components in a PDTS is analyzed. Kinematic chain and corresponding incidence/adjacency matrix are employed to analyze join relationship of PDTS. Properties and simplified rules of kinematic chain are extracted to construct kinematic chain. A procedure for construction kinematic chain of PDTS is then established. Finally, with this two-step approach all 11 rectangular pyramid deployable structures whose folded state is planar are discovered and their kinematic chains are constructed. Based on synthesis results, a novel deployable support structure for satellite SAR is designed. The proposed research can be applied to obtain some novel PDTSs, which is of great importance to design some novel deployable support structures for satellite SAR antenna.

Seismic isolation analysis of FPS bearings in spatial lattice shell structures

A theoretical model of a friction pendulum system （FPS） is introduced to examine its application for the seismic isolation of spatial lattice shell structures. An equation of motion of the lattice shell with FPS bearings is developed. Then, seismic isolation studies are performed for both double-layer and single-layer lattice shell structures under different seismic input and design parameters of the FPS. The influence of frictional coefficients and radius of the FPS on seismic performance are discussed. Based on the study, some suggestions for seismic isolation design of lattice shells with FPS bearings are given and conclusions are made which could be helpful in the application of FPS.