Musk xylene（MX） is frequently used as fragrances in formulation of personal care products. Quantification of a bound 4-amino- MX（4-AMX） as cysteine adducts in trout hemoglobin（Hb） was made by gas chromatography-...Musk xylene（MX） is frequently used as fragrances in formulation of personal care products. Quantification of a bound 4-amino- MX（4-AMX） as cysteine adducts in trout hemoglobin（Hb） was made by gas chromatography-ion trap-mass spectrometry（GC/MS）. The Hb samples were collected from trout after 24 h exposure to MX at 10μg/g, and or menhaden oil（control）. The formation of cysteine-Hb adduct was observed from nitroso derivative of MX, released by alkaline hydrolysis. The released 4-AMX metabolite was extracted in n- hexane. The extract was then reduced by evaporation, and analyzed by GC/MS. When similar agreement of mass spectral features and retention time of 4-AMX were obtained in both standard and sample solutions, the presence of 4-AMX metabolite in the Hb was confirmed. The concentration of 4-AMX was found to be 3.1 × 10^-6 6.9 × 10^-6 mg/g in the Hb solution. Quantitation was made based on an internal standard, a calibration plot, and response factor. In the non-hydrolyzed and laboratory blank extracts, the 4-AMX metabolite was not detected. Additionally, coeluting and interfefing ions were observed in the biological samples.展开更多
文摘Musk xylene（MX） is frequently used as fragrances in formulation of personal care products. Quantification of a bound 4-amino- MX（4-AMX） as cysteine adducts in trout hemoglobin（Hb） was made by gas chromatography-ion trap-mass spectrometry（GC/MS）. The Hb samples were collected from trout after 24 h exposure to MX at 10μg/g, and or menhaden oil（control）. The formation of cysteine-Hb adduct was observed from nitroso derivative of MX, released by alkaline hydrolysis. The released 4-AMX metabolite was extracted in n- hexane. The extract was then reduced by evaporation, and analyzed by GC/MS. When similar agreement of mass spectral features and retention time of 4-AMX were obtained in both standard and sample solutions, the presence of 4-AMX metabolite in the Hb was confirmed. The concentration of 4-AMX was found to be 3.1 × 10^-6 6.9 × 10^-6 mg/g in the Hb solution. Quantitation was made based on an internal standard, a calibration plot, and response factor. In the non-hydrolyzed and laboratory blank extracts, the 4-AMX metabolite was not detected. Additionally, coeluting and interfefing ions were observed in the biological samples.
