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Perfect Pick系统订单拣选策略优化研究
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作者 马云峰 邓力 余佳祥 《物流科技》 2024年第22期1-7,共7页
作为电商仓库作业中耗时最长、成本最高的环节,订单拣选的作业效率一直是仓库管理中的关键问题。文章针对拣选策略选择问题,选择Perfect Pick系统的电商仓库作为背景,对单个拣选台进行研究,提出订单分批和订单排序两种拣选策略并进行对... 作为电商仓库作业中耗时最长、成本最高的环节,订单拣选的作业效率一直是仓库管理中的关键问题。文章针对拣选策略选择问题,选择Perfect Pick系统的电商仓库作为背景,对单个拣选台进行研究,提出订单分批和订单排序两种拣选策略并进行对比分析;以货箱搬运次数最少为目标,分别构建订单分批与订单排序两种拣选策略的整数规划模型,并设计贪婪算法进行求解。通过数值实验验证,订单排序策略在所有订单规模中均优于订单分批策略,根据仓库的订单规模,合理设置拣选台最大容量并选择合理的拣选策略,能够更好地提高拣选效率、优化仓库作业环节。 展开更多
关键词 Perfect pick系统 订单拣选 订单分批 订单排序
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A regression approach for seismic first-break picking
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作者 Huan Yuan San-Yi Yuan +2 位作者 Jie Wu Wen-Jing Sang Yu-He Zhao 《Petroleum Science》 SCIE EI CAS CSCD 2024年第3期1584-1596,共13页
The picking efficiency of seismic first breaks(FBs)has been greatly accelerated by deep learning(DL)technology.However,the picking accuracy and efficiency of DL methods still face huge challenges in low signal-to-nois... The picking efficiency of seismic first breaks(FBs)has been greatly accelerated by deep learning(DL)technology.However,the picking accuracy and efficiency of DL methods still face huge challenges in low signal-to-noise ratio(SNR)situations.To address this issue,we propose a regression approach to pick FBs based on bidirectional long short-term memory(Bi LSTM)neural network by learning the implicit Eikonal equation of 3D inhomogeneous media with rugged topography in the target region.We employ a regressive model that represents the relationships among the elevation of shots,offset and the elevation of receivers with their seismic traveltime to predict the unknown FBs,from common-shot gathers with sparsely distributed traces.Different from image segmentation methods which automatically extract image features and classify FBs from seismic data,the proposed method can learn the inner relationship between field geometry and FBs.In addition,the predicted results by the regressive model are continuous values of FBs rather than the discrete ones of the binary distribution.The picking results of synthetic data shows that the proposed method has low dependence on label data,and can obtain reliable and similar predicted results using two types of label data with large differences.The picking results of9380 shots for 3D seismic data generated by vibroseis indicate that the proposed method can still accurately predict FBs in low SNR data.The subsequent stacked profiles further illustrate the reliability and effectiveness of the proposed method.The results of model data and field seismic data demonstrate that the proposed regression method is a robust first-break picker with high potential for field application. 展开更多
关键词 First-break picking Low signal-to-noiseratio Regression BiLSTM TRAVELTIME Geometry Noisy seismic data
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Automatic velocity picking based on optimal key points tracking algorithm
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作者 Yong-Hao Wang Wen-Kai Lu +3 位作者 Song-Bai Jin Yang Li Yu-Xuan Li Xiao-Feng Gu 《Petroleum Science》 SCIE EI CAS CSCD 2024年第2期903-917,共15页
Picking velocities from semblances manually is laborious and necessitates experience. Although various methods for automatic velocity picking have been developed, there remains a challenge in efficiently incorporating... Picking velocities from semblances manually is laborious and necessitates experience. Although various methods for automatic velocity picking have been developed, there remains a challenge in efficiently incorporating information from nearby gathers to ensure picked velocity aligns with seismic horizons while also improving picking accuracy. The conventional method of velocity picking from a semblance volume is computationally demanding, highlighting a need for a more efficient strategy. In this study, we introduce a novel method for automatic velocity picking based on multi-object tracking. This dynamic tracking process across different semblance panels can integrate information from nearby gathers effectively while maintaining computational efficiency. First, we employ accelerated density clustering on the velocity spectrum to discern cluster centers without the requirement for prior knowledge regarding the number of clusters. These cluster centers embody the maximum likelihood velocities of the main subsurface structures. Second, our proposed method tracks key points within the semblance volume. Kalman filter is adopted to adjust the tracking process, followed by interpolation on these tracked points to construct the final velocity model. Our synthetic data example demonstrates that our proposed algorithm can effectively rectify the picking errors of the clustering algorithm. We further compare the performances of the clustering method(CM), the proposed tracking method(TM), and the variational method(VM) on a field dataset from the Gulf of Mexico. The results attest that our method offers superior accuracy than CM, achieves comparable accuracy with VM, and benefits from a reduced computational cost. 展开更多
关键词 Velocity picking Multi-object tracking Density clustering Kalman filter
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First-Arrival Picking Method for Active Source Data with Ocean Bottom Seismometers Based on Spatial Waveform Variation Characteristics
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作者 LIU Hongwei XING Lei +3 位作者 ZHU Henghua ZHANG Jin ZHANG Jing LIU Huaishan 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第4期970-980,共11页
The precision and reliability of first-arrival picking are crucial for determining the accuracy of geological structure inversion using active source ocean bottom seismometer(OBS)refraction data.Traditional methods fo... The precision and reliability of first-arrival picking are crucial for determining the accuracy of geological structure inversion using active source ocean bottom seismometer(OBS)refraction data.Traditional methods for first-arrival picking based on sample points are characterized by theoretical errors,especially in low-sampling-frequency OBS data because the travel time of seismic waves is not an integer multiple of the sampling interval.In this paper,a first-arrival picking method that utilizes the spatial waveform variation characteristics of active source OBS data is presented.First,the distribution law of theoretical error is examined;adjacent traces exhibit variation characteristics in their waveforms.Second,a label cross-correlation superposition method for extracting highfrequency signals is presented to enhance the first-arrival picking precision.Results from synthetic and field data verify that the proposed approach is robust,successfully overcomes the limitations of low sampling frequency,and achieves precise outcomes that are comparable with those of high-sampling-frequency data. 展开更多
关键词 first-arrival picking spatial waveform variation label cross-correlation superposition method
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Niemann-Pick C1蛋白在埃博拉病毒感染中的作用及其靶向药物研究进展 被引量:1
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作者 吴海燕 陈国江 《中国药理学与毒理学杂志》 CAS 北大核心 2024年第2期153-160,共8页
埃博拉病毒属丝状病毒科,具有高传染性,能引起人类和灵长类动物出现严重出血热等症状,病死率高达90%。Niemann-Pick C1(NPC1)蛋白是埃博拉病毒感染过程中表达于宿主细胞内体膜上的一个重要受体,其与埃博拉病毒被组织蛋白酶裂解的糖蛋白(... 埃博拉病毒属丝状病毒科,具有高传染性,能引起人类和灵长类动物出现严重出血热等症状,病死率高达90%。Niemann-Pick C1(NPC1)蛋白是埃博拉病毒感染过程中表达于宿主细胞内体膜上的一个重要受体,其与埃博拉病毒被组织蛋白酶裂解的糖蛋白(GP)的相互作用是病毒感染宿主的关键环节,介导病毒囊膜与内体膜的融合,进而将病毒基因组释放到宿主细胞。近年来,将NPC1蛋白作为广谱抗丝状病毒药物靶点研发的小分子抑制剂、单克隆抗体和基因治疗药物均有突破性进展。本文介绍了NPC1的结构及其在埃博拉病毒感染中的作用,并对靶向NPC1的小分子抑制剂、单克隆抗体药物和基因治疗药物的研究现状进行总结。 展开更多
关键词 埃博拉病毒 Niemann-pick C1蛋白 小分子抑制剂 抗体 基因治疗
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Manual Order Picking Route Optimization in Distribution Warehouse of Chain Furniture Retail Enterprise
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作者 Yongzhen Zeng Junkun Wang +1 位作者 Ching-Kuei Kao King-Zoo Tang 《American Journal of Operations Research》 2024年第3期105-136,共32页
Due to the effects of the COVID-19 pandemic and the rise of online shopping, the offline sales of IKEA Fuzhou have been declining since 2020. Because the cost of distribution warehouse is a major expense for offline c... Due to the effects of the COVID-19 pandemic and the rise of online shopping, the offline sales of IKEA Fuzhou have been declining since 2020. Because the cost of distribution warehouse is a major expense for offline chain furniture retailers, and the picking process is a key activity in distribution warehouse operations. To reduce the cost of distribution warehouse and alleviate the survival pressure of the offline chain furniture retailers, this paper focuses on optimizing the picking route of the IKEA Fuzhou distribution warehouse. It starts by creating a two-dimensional coordinate system for the storage location of the distribution warehouse using the traditional S-type picking strategy to calculate the distance and time of the sorting route. Then, the problem of optimizing the picking route is then transformed into the travelling salesman problem (TSP), and picking route optimization model is developed using a genetic algorithm to analyze the sorting efficiency and picking route optimization. Results show that the order-picking route using the genetic algorithm strategy is significantly better than the traditional S-type picking strategy, which can improve overall sorting efficiency and operations, reduce costs, and increase efficiency. Thus, this establishes an implementation process for the order-picking path based on genetic algorithm optimization to improve overall sorting efficiency and operations, reduce costs, increase efficiency, and alleviate the survival pressure of pandemic-affected enterprises. 展开更多
关键词 S-Shaped picking Strategy picking Route Traveling Salesman Problem Genetic Algorithm
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Basic Research on the Burden of Dropping off and Picking up Children: Differences by Employment Type and Gender in the Tokyo Metropolitan Area
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作者 Jun Morio Koshi Isono +1 位作者 Masahiko Kikuchi Tetsuo Morita 《Journal of Transportation Technologies》 2024年第3期445-461,共17页
There is a need to reduce the burden of child drop-off and pick-up for child-rearing generations, but most studies on the actual situation in Japan are based on survey results. In this study, we analyzed differences i... There is a need to reduce the burden of child drop-off and pick-up for child-rearing generations, but most studies on the actual situation in Japan are based on survey results. In this study, we analyzed differences in child drop-off and pick-up by employment type and gender, utilizing the “Metropolitan Area Person Trip Survey,” which is a statistical data set. The study targeted households in which both spouses were between 30 and 49 years old, had children under the age of 6, and included the following three groups. 1) Dual-income Group 1 (both spouses employed/on contract/temporary);2) Dual-income Group 2 (husband employed/on contract/temporary, wife part-time);3) Full-time housewife group (husband employed, wife unemployed). The analysis revealed that a) wives are almost always responsible for dropping off and picking up their children;b) husbands drop off and pick up their children less frequently in dual-income households;and c) households with children raising within 10 to 30 km of Tokyo Station have longer commuting times and need to reduce the burden of dropping off and picking up their children. 展开更多
关键词 Drop-Off and pick-Up Employment Type GENDER Dual-Income Households Tokyo Metropolitan Area
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基于双目视觉技术的茶叶嫩芽定位方法研究
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作者 朱龙舜 郑旻璐 《农机化研究》 北大核心 2025年第2期49-53,共5页
进一步提升茶叶嫩芽定位的准确性及茶叶嫩芽采摘的作业效率,选取双目视觉技术作为切入点,针对其识别、定位、采摘等环节展开设计研究。考虑茶叶嫩芽采摘的基础特征和双目视觉技术的应用条件,合理搭建双目视觉模型,选用立体匹配的核心定... 进一步提升茶叶嫩芽定位的准确性及茶叶嫩芽采摘的作业效率,选取双目视觉技术作为切入点,针对其识别、定位、采摘等环节展开设计研究。考虑茶叶嫩芽采摘的基础特征和双目视觉技术的应用条件,合理搭建双目视觉模型,选用立体匹配的核心定位算法,完成茶叶嫩芽的定位方法与采摘功能优化,并布置相应的试验机构,展开定位采摘验证试验。结果表明:基于双目视觉技术的茶叶嫩芽定位方法应用具有显著的优越性,较普通定位方法而言,识别误匹配率可降低至4.01%,平均漏采率为3.20%,嫩芽采摘完整率达93.40%,可以很好地促进茶叶嫩芽的后续处理加工,是采摘茶叶嫩芽的品质化的重要基础保障。 展开更多
关键词 茶叶嫩芽定位 双目视觉 立体匹配 采摘完整率 品质化
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Pick病的诊断 被引量:3
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作者 贾建军 卢文甫 +2 位作者 王鲁宁 汤洪川 尹岭 《中国医学影像技术》 CSCD 2001年第4期318-320,共3页
目的 探讨Pick病 (Pickdisease)诊断的正确性。方法 分析 2例临床上曾诊断为阿尔茨海默病(Alzheimerdisease,AD) ,经核磁共振成像 (MRI)检查证实的Pick病 ,其中 1例经正电子发射型计算机断层扫描 (PET)得到进一步证实。结果 Pick病... 目的 探讨Pick病 (Pickdisease)诊断的正确性。方法 分析 2例临床上曾诊断为阿尔茨海默病(Alzheimerdisease,AD) ,经核磁共振成像 (MRI)检查证实的Pick病 ,其中 1例经正电子发射型计算机断层扫描 (PET)得到进一步证实。结果 Pick病与AD比较有如下特点 :①特殊的语言方式及语言改变 ;②临床上以缓慢进展的性格改变及社会活动能力衰退为主 ,而记忆力、理解判断力、计算力障碍等出现相对较晚 ;③病程中有不同程度的Kl櫣ver Bucy综合征表现 ;④脑MRI检查提示 :额和 (或 )颞叶萎缩 ,颞极为著 ;⑤PET检查提示 :双侧额、颞叶代谢减低 ,颞极明显。结论 根据Pick病临床表现及神经影像学特征 ,在排除AD及其它脑变性病的基础上 ,生前诊断Pick病是可能的。 展开更多
关键词 pick 阿尔茨海默病 磁共振成像 正电子发射型计算机断层扫描 诊断
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Pick病的脑MRI研究 被引量:1
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作者 卢文甫 王鲁宁 +2 位作者 贾建军 汤洪川 蔡幼铨 《中国医学影像技术》 CSCD 2001年第10期945-947,共3页
目的 研究Pick病患者脑萎缩变化的特点 ,并动态研究脑萎缩变化的程度和病程及临床之间的相互相关系。方法  2例Pick病患者分别进行 5次和 7次脑MRI动态观察 ,并和 6例Alzheimer病患者进行对比分析。结果 Pick病早期以Kluver Bacy综... 目的 研究Pick病患者脑萎缩变化的特点 ,并动态研究脑萎缩变化的程度和病程及临床之间的相互相关系。方法  2例Pick病患者分别进行 5次和 7次脑MRI动态观察 ,并和 6例Alzheimer病患者进行对比分析。结果 Pick病早期以Kluver Bacy综合征和情感障碍为突出 ,人格改变、行为异常、语言障碍为显著 ,而智能减退相对较轻较晚 ,脑MRI以额或 /和颞极萎缩为主 ,尤其颞上回前 1/ 3为明显 ,其脑MRI的改变和临床特征及病程相一致。“刀片”状、“剪刀”像和“锥柱”样或“蘑菇”状脑萎缩改变可能是Pick病的特点。结论 充分认识这些特征 ,以利于Pick病的早期临床诊断。 展开更多
关键词 pick MRI 诊断
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Pick病3例临床及病理分析 被引量:1
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作者 卢文甫 王鲁宁 +2 位作者 宋康兴 贾建华 汤洪川 《解放军医学杂志》 CAS CSCD 北大核心 2001年第11期847-849,共3页
通过分析 3例Pick病的临床、脑MRI、SPECT、PET和脑病理资料 ,发现和Alzheimer病相比 ,Pick病早期以人格变化、判断力改变较为特异 ,情感、情绪变化明显 ,Kl櫣ver Bucy综合征为特征 ,非流利性失语和语义性记忆障碍突出 ,而智能减退... 通过分析 3例Pick病的临床、脑MRI、SPECT、PET和脑病理资料 ,发现和Alzheimer病相比 ,Pick病早期以人格变化、判断力改变较为特异 ,情感、情绪变化明显 ,Kl櫣ver Bucy综合征为特征 ,非流利性失语和语义性记忆障碍突出 ,而智能减退及视觉空间定向能力受损相对较晚 ,脑MRI以颞极萎缩为重 ,SPECT和PET以额和 (或 )颞叶低灌流及低代谢为显著。提示应充分认识Pick病的特点 。 展开更多
关键词 临床病理学 脑皮克病 pick 早期诊断
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PICK1蛋白生理功能及其作为药物新靶点研究进展 被引量:2
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作者 段贤春 汪永忠 +9 位作者 高家荣 冯艺戎 李翔 吴健 刘晓闯 魏良兵 何云娇 朱红 李瑛 夏伦祝 《中国药理学通报》 CAS CSCD 北大核心 2013年第11期1606-1610,共5页
PICK1蛋白(protein interacting with C alpha kinase 1)是一种同时具有PDZ和BAR区域的支架蛋白,在哺乳动物体内与多种蛋白质相互作用,并被证明在多种生理过程中发挥重要的调节作用,同时参与了多种疾病病理过程。因此,PICK1蛋白可能成... PICK1蛋白(protein interacting with C alpha kinase 1)是一种同时具有PDZ和BAR区域的支架蛋白,在哺乳动物体内与多种蛋白质相互作用,并被证明在多种生理过程中发挥重要的调节作用,同时参与了多种疾病病理过程。因此,PICK1蛋白可能成为极具前景的疾病治疗靶点。该文通过对近年来国内外发表的相关文献进行整理与分析,综述了PICK1蛋白的生理功能与其作为药物靶点的研究新进展,旨在为PICK1蛋白的深入研究提供理论支持。 展开更多
关键词 pick1 PDZ结构域 生理功能 中风 脑病 药物靶点
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PICK1:一个功能多样的药靶蛋白 被引量:2
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作者 宋婀莉 高友鹤 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2007年第9期697-705,共9页
蛋白质是生命功能的执行者.生命体中某些关键蛋白的功能异常往往是导致疾病发生的根本原因.这些疾病相关蛋白极有可能成为药物靶点,为新药研发和疾病治疗提供重要线索.PICK1蛋白(protein interacting with Cαkinase1)结合能力广泛、功... 蛋白质是生命功能的执行者.生命体中某些关键蛋白的功能异常往往是导致疾病发生的根本原因.这些疾病相关蛋白极有可能成为药物靶点,为新药研发和疾病治疗提供重要线索.PICK1蛋白(protein interacting with Cαkinase1)结合能力广泛、功能多样以及在多种重要疾病(如:癌症、精神分裂症、疼痛、帕金森综合症等)的发生发展过程中发挥潜在的作用,使其成为一个可能的药靶蛋白.PICK1与绝大多数配体蛋白的相互作用是通过其PDZ结构域与配体C末端区域的结合介导的,使PICK1的PDZ结构域成为一个潜在的药物靶点.因此,可以利用生物小分子物质特异性地结合PICK1的PDZ结构域,干扰或阻断PICK1与配体蛋白的天然相互作用,最终达到治疗相关疾病的目的. 展开更多
关键词 pick1(protein INTERACTING with Cαkinase 1) PDZ结构域 药物靶点 阻断性多肽
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矩阵值Caratheodory函数广义块Pick矩阵的秩不变性 被引量:1
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作者 贺勤 徐清舟 陈公宁 《数学物理学报(A辑)》 CSCD 北大核心 2012年第1期103-112,共10页
Lasarow推导出矩阵值Caratheodory函数的第一、第二型广义块Pick矩阵及其变型的秩不变性.这些矩阵由同一个Caratheodory函数的值与它的直到某阶的导数值确定.利用文献中提出的块Toeplitz向量方法,该文断言,这些块矩阵的秩分别相关并重... Lasarow推导出矩阵值Caratheodory函数的第一、第二型广义块Pick矩阵及其变型的秩不变性.这些矩阵由同一个Caratheodory函数的值与它的直到某阶的导数值确定.利用文献中提出的块Toeplitz向量方法,该文断言,这些块矩阵的秩分别相关并重合于具有秩不变性的块Toeplitz矩阵的秩,从而改进了这两类广义块Pick矩阵的秩不变性结论的证明. 展开更多
关键词 q×q CARATHEODORY函数 广义块pick矩阵 块Toeplitz矩阵 秩不变性.
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广义块Pick型矩阵和带导数的Nevanlinna-Pick矩阵插值问题 被引量:1
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作者 陈公宁 胡永建 《北京师范大学学报(自然科学版)》 CAS CSCD 北大核心 1998年第3期305-312,共8页
建立了广义块Pick型矩阵和块Toeplitz矩阵之间的一种等价关系。
关键词 广义块pick 矩阵 块Toeplitz矩阵 N-P插值
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Landscape Design of Sightseeing and Picking Garden Transformed from Vegetable Producing Garden 被引量:1
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作者 孙明德 曹均 《Journal of Landscape Research》 2011年第5期4-7,共4页
In view of landscape design problems in the transition from vegetable producing garden to sightseeing and picking garden,definitions of both gardens were introduced and discriminated.It was proposed that landscapes in... In view of landscape design problems in the transition from vegetable producing garden to sightseeing and picking garden,definitions of both gardens were introduced and discriminated.It was proposed that landscapes in the vegetable sightseeing and picking garden included installations,open-field vegetable producing landscapes and overall environment landscapes.Landscape design concepts and principles of vegetable sightseeing and picking garden were analyzed,and it was stressed that its landscape design should take quality production of vegetables and fruits as the principal line,environment landscapes of the garden as the support,and experiencing production process as the feature,by following the principles of "integrity of garden design,characteristic vegetable varieties,proper crop rotation,ecological production process".Landscape contents of this garden were analyzed from 3 perspectives:landscape design within installations,major road,and overall appearance of the garden.Cangshang Vegetable Sightseeing and Picking Garden in Beiwu Township,Shunyi District,Beijing City was taken for an example to analyze its landscape construction inside and outside greenhouses as well as the optimization of the overall environment landscapes on the basis of introducing its landscape design concepts. 展开更多
关键词 VEGETABLE GARDEN SIGHTSEEING and pickING LANDSCAPE DESIGN
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额颞痴呆与Pick病 被引量:2
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作者 孟红旗 全亚萍 《佛山科学技术学院学报(自然科学版)》 CAS 2010年第1期76-79,共4页
额颞痴呆(frontotemporal dementia,FTD)是以额颞叶萎缩为特征的一组神经变性疾病。病因认为是轴索病变继发神经细胞改变,机制为常染色体显性遗传。病理为双侧额叶,颞叶前端的局限性萎缩,可累及尾状核、壳核、丘脑、黑质等皮质下结构。... 额颞痴呆(frontotemporal dementia,FTD)是以额颞叶萎缩为特征的一组神经变性疾病。病因认为是轴索病变继发神经细胞改变,机制为常染色体显性遗传。病理为双侧额叶,颞叶前端的局限性萎缩,可累及尾状核、壳核、丘脑、黑质等皮质下结构。起病隐袭,早期症状为行为、人格改变,渐进性发展为认知功能损害,迅速进展为语言障碍,晚期通常沉默不语,不能与人交流,可有行为异常。MRI显示额叶、前颞叶萎缩。确诊依赖于病理学证据,如局限性额颞叶萎缩,神经元和神经胶质发现tau蛋白包涵体及Pick小体细胞。目前无特异性治疗的治疗方法。 展开更多
关键词 额颞痴呆 pick 额颞叶萎
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PICK1在肝纤维化中的表达变化及功能研究 被引量:1
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作者 陈培杰 李俊 +2 位作者 黄成 孟晓明 蔡双朋 《安徽医科大学学报》 CAS 北大核心 2015年第11期1606-1610,共5页
目的研究蛋白激酶C结合蛋白1(PICK1)在小鼠肝纤维及活化的肝星状细胞中的表达变化,并探讨其对人肝星状细胞株(LX-2)活化的影响。方法建立四氯化碳(CCl4)诱导的小鼠肝纤维化模型,观察PICK1在肝纤维化过程中的表达变化;转化生长因子β1(T... 目的研究蛋白激酶C结合蛋白1(PICK1)在小鼠肝纤维及活化的肝星状细胞中的表达变化,并探讨其对人肝星状细胞株(LX-2)活化的影响。方法建立四氯化碳(CCl4)诱导的小鼠肝纤维化模型,观察PICK1在肝纤维化过程中的表达变化;转化生长因子β1(TGF-β1)刺激LX-2活化,Western blot测定PICK1的蛋白表达情况;转染PICK1过表达质粒至LX-2中,再以TGF-β1诱导活化,Western blot检测PICK1、α平滑肌肌动蛋白(α-SMA)、I型胶原(Col1a1)和Smad2、3及其磷酸化水平的蛋白表达情况。结果正常组小鼠肝脏中PICK1表达较高,随着肝纤维化的加重,PICK1的表达逐渐递减。TGF-β1诱导活化的LX-2细胞中PICK1表达降低。转染PICK1过表达质粒后,TGF-β1诱导的α-SMA、Colla1的表达明显减少,且Smad2、3磷酸化水平显著下降。结论 PICK1在纤维化肝组织及活化的HSC中表达下调。过表达PICK1可抑制TGF-β1诱导的LX-2活化,可能是通过抑制TGF-β/Smad通路而发挥作用,为肝纤维化的防治研究提供了新的思路和靶点。 展开更多
关键词 pick1 肝纤维化 肝星状细胞
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基于SCARA的柔性葡萄采摘机械臂设计与试验 被引量:1
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作者 杨语录 李长勇 《农机化研究》 北大核心 2025年第1期72-78,86,共8页
针对现今葡萄种植环境狭小且复杂、常见关节型机械臂空间运动灵活度较小和避障能力较弱等问题,为了适应葡萄植株下层枝叶较少、障碍物较少,上层枝叶较多、障碍物较多的特点,设计并开发一款基于SCARA的柔性葡萄采摘机械臂,其底部为SCARA... 针对现今葡萄种植环境狭小且复杂、常见关节型机械臂空间运动灵活度较小和避障能力较弱等问题,为了适应葡萄植株下层枝叶较少、障碍物较少,上层枝叶较多、障碍物较多的特点,设计并开发一款基于SCARA的柔性葡萄采摘机械臂,其底部为SCARA机械臂,上部为柔性机械臂部分;同时,设计了SCARA与柔性机械臂的协调运动控制系统。通过分析机械臂的机械结构和工作原理确定机械臂的末端空间位置和机械臂的运动学关系,利用MatLab绘制其工作空间,并绘制柔性机械臂在随机两个空间点运动的轨迹、速度、加速度图,从而实现SCARA机械臂与柔性机械臂的运动控制。 展开更多
关键词 采摘 柔性机械臂 运动空间 运动学求解 SCARA机械臂 葡萄
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PICK1对人肝癌细胞HepG2增殖的影响 被引量:1
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作者 谢娟 周群 +3 位作者 刘雪姣 黄成 孟晓明 李俊 《安徽医科大学学报》 CAS 北大核心 2016年第5期615-619,共5页
目的检测蛋白激酶Cα相互作用蛋白1(PICKl)在肝癌、癌旁组织中的表达,初步探讨PICK1对人肝癌细胞HepG2增殖的作用及其可能机制。方法应用qRT-PCR、Western blot、HE和免疫组织化学染色法分析比较人肝癌和癌旁组织中PICK1蛋白的表达;培养... 目的检测蛋白激酶Cα相互作用蛋白1(PICKl)在肝癌、癌旁组织中的表达,初步探讨PICK1对人肝癌细胞HepG2增殖的作用及其可能机制。方法应用qRT-PCR、Western blot、HE和免疫组织化学染色法分析比较人肝癌和癌旁组织中PICK1蛋白的表达;培养HepG2细胞,体外给予不同浓度的FSC-231(PICK1的PDZ结构域小分子抑制剂),通过MTT法检测HepG2细胞的活力;采用流式细胞术检测HepG2细胞周期变化;进一步采用Western blot法检测G1/S-特异性周期蛋白-D1(CyclinD1)、原癌基因C-myc和Notch信号通路蛋白表达。结果肝脏病理组织学检测提示肝癌组织病变明显。免疫组化结果显示,肝癌组织中相比癌旁组织中的PICKl抗原阳性细胞显著增多(P<0.05),且多分布在肝实质细胞质区域;qRT-PCR和Western blot结果显示肝癌组织中PICK1 mRNA及蛋白水平与癌旁组相比显著升高(P<0.05);体外实验证明,与阴性对照组相比,FSC-231能够抑制HepG2细胞的增殖,并且明显抑制CyclinD1、C-myc和Notch1同源蛋白1(Notch1)、Hes-1蛋白表达(P<0.05)。结论提示PICK1可能参与肝癌的发生发展,PICK1的PDZ结构域抑制剂可以抑制肝癌细胞的增殖,此作用可能与Notch信号通路相关。 展开更多
关键词 pick1 FSC-231 HEPG2细胞 肝癌 肝细胞增殖 CYCLIND1 NOTCH-1 Hes-1
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