Latest development of display technologies

In this review we will focus on recent progress in the field of two-dimensional（2D） and three-dimensional（3D）display technologies.We present the current display materials and their applications,including organic light-emitting diodes（OLEDs）,flexible OLEDs quantum dot light emitting diodes（QLEDs）,active-matrix organic light emitting diodes（AMOLEDs）,electronic paper（E-paper）,curved displays,stereoscopic 3D displays,volumetric 3D displays,light field3 D displays,and holographic 3D displays.Conventional 2D display devices,such as liquid crystal devices（LCDs） often result in ambiguity in high-dimensional data images because of lacking true depth information.This review thus provides a detailed description of 3D display technologies.

Recent progress in microbial cell surface display systems and their application on biosensors

Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.

Isolation of Goose-origin scFv Antibodies Against Goose Parvovirus from Bacterial Display Antibody Libraries

Goose parvovirus(GPV)can cause a highly contagious and fatal gosling plague(GP)disease in goslings and muscoy ducklings.Here,three goose-origin neutralizing single chain variable fragment(scFv)antibodies against GPV SYG-61 were isolated.The genes of scFv antibodies were derived from goslings immunized with GPV SYG-61,and scFvs were subcloned into a pBSD vector for the construction of pBSD-scFv libraries.The pBSD-scFv libraries were screened following three rounds using VP2(protective antigen of GPV)as the bait by flow cytometry(FCM).After screening,the 15 clones with high mean fluorescence intensity(MFI)were isolated and sequenced.These 15 scFvs were expressed by pET-28a(+)in E.coli.The specificity and affinity of the 15 purified scFvs were successfully confirmed by ELISA.In the preliminary neutralization experiment on primary goose embryo fibroblast(GEF)in vitro,three of the 15 purified scFvs(named scFv-10,scFv-11 and scFv-50)showed significant neutralizing capacities.The study generated the first goose-origin neutralizing scFv against GPV and laid the foundation for the appearance of full-length goose-origin neutralizing monoclonal antibody against GPV.

Cell Surface Display of Red-Grouper Nervous Necrosis Virus Capsid Protein on <i>Pichia pastoris</i>

Nervous necrosis virus (NNV), the etiological agent of viral nervous necrosis, has a high mortality rate of 100% in hatchery-reared larvae and juveniles. At present, there are still no effective vaccines available for NNV. Pichia pastoris surface display of viral capsid proteins was generated in hopes of developing an oral vaccine against red-grouper-nervous-necrosis virus (RGNNV) in fish. Fingerlings or juveniles that showed clinical signs of NNV infection were proved by RT-PCR for the appearance of expected length of 198 bpcDNA and further analysis by DNA sequencing. The DNA fragment containing AGα1 linked to RG-NNVRNA2, 2100 bp in length, was inserted into pPIC9K vector. Linearlized plasmids were electroporated into P. pastoris GS115 (mut+His?) and yeast isolates that had Muts?His+ and resistance phenotype at 4 mg/mL geniticin were selected to determine the integration of the target gene by PCR reaction. The extracted cell walls from the yeasts cultured in buffered-methanol-complex medium (BMMY) through an induction of 0.5% methanol for 6 days, were investigated for the fusion proteins by western blot. A protein band of 73 kDa predicted to be the fusion protein and a non-specific one of 56 kDa were detected. Staining of the fusion proteins expressing cells with corresponding antibodies revealed their presence of NNVRNA2, but varied the intensity of detected signals from cell to cell by confocal laser scanning fluorescence microscopy. The predicted fusion proteins tertiary structure also confirmed exposed conformation of the fusion protein on the cell wall. In this study, the capsid proteins from the red-spotted grouper nervous necrosis virus were successfully expressed on the cell surface of P. pastoris but still low levels of fusion protein expression. Further studies are required to optimize fully surface protein expression prior to evaluate the possible use of the constructed recombinant yeast as an oral vaccine against RG-NNV infection.

Application of Quantum Dots in Display:The Milestones of Quantum Dots Light Emitting Displays

We reviewed the key advantages and development of the QD-display and other light applications based on their color purity,stability,and solution processisibility.Analysis of quantum dot based LEDs and the main challenges facing in this field,such as QD luminescence quenching,QD charging in thin films,and the external quantum efficiency was presented in detail.The description about how different optical down-conversion and structures enabled researchers to overcome these challenges and to commercialize the products to achieve the desirable CRI and color temperature was presented.The recent developments about how to overcome these difficulties have also been discussed in this article.

Display纸张化的大趋势——Microcup(微杯)电子纸及其应用

“电子纸”的概念,其实早在70年代初便已萌芽;据闻是因为当时阴极射线管太过模糊、计算机屏幕品质太差所意外引爆的火花。而电泳显示(EPD,ElectroPhoretic Display,俗称Electronic Paper电子纸)是一新颖的光电显示技术,目前全球仅美、日两国有计划地投入研发生产工作;1999年诞生于美国加州硅谷(San Jose)、2004年回师台湾地区成立营运总部的锣洤科技（ＳｉｐｉｘＴｅｃｈｎｏｌｏｇｙＩｎｃ．），其ＥＰＤ技术已被美国、日本等国际大厂公认为“可挠式电子纸显示器”的世界先驱。

虚拟现实系统及应用

虚拟现实技术是一种模拟人在自然环境中的听、视、动等行为的高级互动技术.虚拟现实系统通过头盔方式的三维立体显示器、数据手套及立体声耳机等设备使人能完全沉浸在计算机创造的图形世界里.它是人工智能、计算机图形学、人机接口技术、网络技术、并行技术等多种技术的集成.目前已在科学可视化、飞行模拟、医学、CAD CAM、教育、娱乐等领域获得了广泛的应用.

牛体外发育胚胎特定阶段差异表达基因的研究(英文)

[目的]研究不同发育时期牛体外受精胚胎在基因表达模式上的差异。[方法]利用单个胚胎mRNA差异显示技术,对单个8细胞期胚胎与囊胚进行mRNA差异显示,获得1条特异表达条带,对其进行克隆、测序,并与GenBank进行对比。[结果]该序列与牛核糖体蛋白L31基因(ribosom alprotein L31,RPL31)具有99%的同源性。采用实时定量PCR技术检测8细胞期和囊胚期胚胎RPL31的mRNA表达量,结果表明,RPL31在8细胞期胚胎的相对表达量为囊胚期胚胎的3.2倍。[结论]为揭示和阐明控制牛早期胚胎发育的相关机理提供依据。

Screening and evaluation of human single-chain fragment variable antibody against hepatitis B virus surface antigen

BACKGROUND: Phage display technology has become a vital tool in studies aimed at identifying molecules binding to a specific target. It enables the rapid generation and selection of high affinity, fully human antibody product candidates to essentially any disease target appropriate for antibody therapy. In this study, we prepared the recombinant single-chain fragment variable ( ScFv) antibody to hepatitis B virus surface antigen (HBsAg) by the phage display technology for obtaining a virus-targeting mediator. METHODS: mRNA was isolated from B-lymphocytes from a healthy volunteer and converted into cDNA. The fragment variables of heavy and light chain were amplified separately and assembled into ScFv DNA with a specially constructed DNA linker by polymerase chain reaction. The ScFv DNA was ligated into the phagmid vector pCANT-AB5E and the ligated sample was transformed into competent E. coli TG1. The transformed cells were infected with M13K07 helper phage to form a human recombinant phage antibody library. The volume and recombinant rate of the library were evaluated by bacterial colony count and restriction analysis. After two rounds of panning with HBsAg. the phage clones displaying ScFv of the antibody were selected by enzyme-linked immunosorbant assay ( ELISA) from the enriched phage clones. The antigen binding affinity of the positive clone was detected by competition ELISA. HB2151 E. coli was transfected with the positive phage clone demonstrated by competition ELISA for production of a soluble form of the anti-HBsAg ScFv. ELISA assay was used to detect the antigen binding affinity of the soluble anti-HBsAg ScFv. Finally, the relative molecular mass of soluble anti-HBsAg ScFv was measured by SDS-PAGE. RESULTS: The variable heavy ( VH ) and variable light (VL) and ScFv DNAs were about 340bp, 320bp and 750bp, respectively. The volume of the library was up to 2 × 106 and 8 of 10 random clones were recombinants. Two phage clones could strongly compete with the original HBsAb for binding to HBsAg. Within 2 strong positive phage clones, the soluble anti-HBsAg ScFv from one clone was found to have the binding activity with HBsAg. SDS-PAGE showed that the relative molecular weight of soluble anti-HBsAg ScFv was 32 kDa. CONCLUSION: The anti-HBsAg ScFv successfully produced by phage antibody technology may be useful for broadening the scope of application of the antibody.

Three-dimensional display technologies in wave and ray optics: a review(Invited Paper)

Multiple three-dimensional （3D） display technologies are reviewed. The display mechanisms discussed in this paper are classified into two categories： holographic display in wave optics and light field display in ray optics, which present the 3D optical wave field in two different ways. Key technical characteristics of the optical systems and the depth cues of human visual system are analyzed. It is to be expected that these 3D display technologies will achieve practical applications with the increase of the optical system bandwidth.

Emerging Technologies of Liquid Crystal Displays

The general features and the emerging technologies of liquid crystal displays are described from the viewpoints of wide viewing and fast response technologies. The device applications of liquid crystals for optical communications are also described.

Technical analysis of 3DTV and outstanding issues

This paper analyzes the technical characteristic of three-dimensional display technology （3DTV） system and some core technologies yet to be solved. It points out the ways to solve these problems and presents an effective solution for thediscomfort of watching the three-dimensional TV.

Application of M13 Phage Coat Proteins

M13 phage＇is a filamentous bacteriophage containing a circular single-stranded DNA molecular, which is surrounded by approximately 2 800 copies of protein PVIII. In addition, there are five copies of each of proteins PVII and PXI in one end, and five copies of each of proteins PVI and PUI on the other. These coat proteins have play an important role in the infection and assembly of M13 phage. With the development of phage display technology, these five coat proteins all can be used in phage display, which plays an increasingly important role in molecular detection and treatment.

Single Chain Fragment Variables Antibody binding to EGF Receptor in the Surface of MCF7 Breast Cancer Cell Line: Application and Production Review

In this review, single-chain fragment variable construction using phage-display technology as a promising anticancer immunotherapy technology is described. Cloning and the specific bio-panning selection with phage display technology, as well as the use of the epidermal growth factor receptor (EGFR) at the surface of MCF-7 cells as the antigen for the straightforward specific selection of single chain Fvs, are discussed. Moreover, phage display technologies and their application are important for vaccine production and immunotherapy against viruses and cancers. Furthermore, expression of the gene will cause the production and expression of the protein in prokaryotic and eukaryotic cells, which can be used to detect anti-cancer single chain fragment variables (scFvs). Finally, homology modelling is described to show the three-dimensional scFv structure that verifies the Complementary-Determining-Regions (CDRs) on the surface of the model.

Assistive Navigation Device for Visually Impaired—A Study on Reaction Time to Tactile Modality Stimuli

A tactile system to support severe visually-impaired or blind people in the world for their orientation and navigation had been developed. To optimize the design, some parameters of tactile display device were evaluated. In the present paper,we focused on the reaction time to tactile stimuli. In the test, the stimuli were produced through a vibration belt that was worn around the participants’ waist. In the choice reaction time task, the participants had to click corresponding arrow keys according to the location of a tactile signal. The findings of this study provided a reference of the reaction time range, so as to design a more effective and safe tactile navigation system.

商標評審、訴訟實例

“i-Module”商标 韩国申请人株式会社现代显示科技（Hyundai Display Technology，Inc．）於2001年9月PP日在国际分类第9类“液晶显示监视器（计算机硬件）”商品上向商标局提出“i-Module”商标（以下称申请商标，见附图P）的注册申请，并声明指定颜色’。商标局经审查，於2002年7月2日以ZC1985099BH1号商标驳回通知书驳回了该商标的注册申请，理由是申请商标与微软公司（Microsoft Corporation）在类似商品上已注册的第138741、号“i”商标（以下称引证商标，见附图2，刊登於第720期《商标公告》）近似，且该商标中的“Module”译为“模数、模块”，为计算机技术术语，在指定商品上用作商标缺乏显着特征。

Apollo公司LVDS到TTL适配器板

Apollo Display Technologies公司的Whisper 3000系列LVDS到TTL适配器板（P／N：CDS-LVDS-VGA）可以将标准TTL LVGA显示器接口转换为低辐射LVDS接口。据称它可以将VGA TTL显示器从18″扩展到3m。单通道LVDS 18位输入能够方便地连接到大多数控制器和SBC。广泛的工作温度范围使该适配器板既适合消费类LCD使用，又适合工业LCD使用。

Phage-displayed heptapeptide sequence conjugation significantly improves the specific targeting ability of antimicrobial peptides against Staphylococcus aureus

Broad-spectrum antibacterial drugs often lack specificity,leading to indiscriminate bactericidal activity,which can disrupt the normal microbial balance of the host flora and cause unnecessary cytotoxicity during systemic administration.In this study,we constructed a specifically targeted antimicrobial peptide against Staphylococcus aureus by introducing a phage-displayed peptide onto a broad-spectrum antimicrobial peptide and explored its structure–function relationship through one-factor modification.SFK2 obtained by screening based on the selectivity index and the targeting index showed specific killing ability against S.aureus.Moreover,SFK2 showed excellent biocompatibility in mice and piglet,and demonstrated significant therapeutic efficacy against S.aureus infection.In conclusion,our screening of phage-derived heptapeptides effectively enhances the specific bactericidal ability of the antimicrobial peptides against S.aureus,providing a theoretical basis for developing targeted antimicrobial peptides.

Enhanced photoluminescence of a microporous quantum dot color conversion layer by inkjet printing

Owing to their high color purity,tunable bandgap,and high efficiency,quantum dots(QDs)have gained significant attention as color conversion materials for high-end display applications.Moreover,inkjet-printed QD pixels show great potential for realizing full-color mini/micro-light emitting diode(micro-LED)-based displays.As a color conversion layer,the photoluminescence intensity of QDs is limited by the insufficient absorptance of the excitation light due to the lack of scattering.Conventional scatterers,such as titanium dioxide microparticles,have been applied after additional surface engineering for sufficient dispersity to prevent nozzle clogging in inkjet printing process.In our work,as an alternative approach,we use inkjet printing for depositing a phase separating polymer ink based on polystyrene(PS)and polyethylene glycol(PEG).QD/polymer composite pixels with scattering micropores are realized.The morphology of the micropores can be tailored by the weight ratio between PS and PEG which enables the manipulation of scattering capability.With the presence of the microporous structure,the photoluminescence intensity of the QD film is enhanced by 110%in drop-cast films and by 35.3%in inkjet-printed QD pixel arrays compared to the reference samples.