Study on Extraction of Oolong Tea Assisted by Ultrasonic Wave and 4C Technique and Its Application

[Objectives]The extraction conditions of formula oolong tea were investigated by an orthogonal experiment.[Methods]The technical conditions were optimized by the 4C method,and the application of formula oolong tea extract in cigarettes was studied.[Results]①In the experimental range,the best sensory evaluation effect of formula oolong tea extract was obtained with extraction conditions of 70%ethanol as extraction solvent,extraction time h,extraction temperature 25℃,and ultrasonic frequency 80 kHz,and follow-up low-temperature concentration,low-temperature sedimentation and low-temperature centrifugation.②The effects of different centrifugal speeds on the quality of formula oolong tea extract were explored.The formula oolong tea extract obtained under the conditions of 3000 r/min and centrifugal time of 10 min showed the best evaluation effect with soft and delicate smoke,rich smoke fragrance,good comfort and refreshing mouthfeel.③The effective aroma components in the formula oolong tea extract were qualitatively analyzed by GC-MS.[Conclusions]This study provides high-quality raw materials and a theoretical basis for the research of independent flavor blending in cigarette industry enterprises.

Pilot study on the effect of flavonoids on arterial stiffness and oxidative stress in chronic kidney disease

BACKGROUND Flavonoids,the main class of polyphenols,exhibit antioxidant and antihypertensive properties.AIM To prospectively investigate the impact of flavonoids on arterial stiffness in patients with chronic kidney disease(CKD)stagesⅠ-Ⅳ.METHODS In this prospective,single-arm study,CKD patients with arterial hypertension and diabetes mellitus were enrolled.Baseline demographic,clinical,and laboratory variables were recorded.Patients received daily treatment with a phenol-rich dietary supplement for 3 months.Blood pressure,arterial stiffness(carotidfemoral pulse wave velocity,central pulse pressure),and oxidative stress markers(protein carbonyls,total phenolic compound,total antioxidant capacity)were measured at baseline and at study end.RESULTS Sixteen patients(mean age:62.5 years,87.5%male)completed the study.Following intervention,peripheral systolic blood pressure decreased significantly by 14 mmHg(P<0.001).Carotid-femoral pulse wave velocity decreased from 8.9 m/s(baseline)to 8.2 m/s(study end)(P<0.001),and central pulse pressure improved from 59 mmHg to 48 mmHg(P=0.003).Flavonoids also reduced oxidative stress markers including protein carbonyls(P<0.001),total phenolic compound(P=0.001),and total antioxidant capacity(P=0.013).CONCLUSION Flavonoid supplementation in CKD patients shows promise in improving blood pressure,arterial stiffness,and oxidative stress markers.

A comparative study for determining rock joint normal stiffness with destructive uniaxial compression and nondestructive ultrasonic wave testing

Rock joints are one of the vital discontinuities in a natural rock mass.How to accurately and conveniently determine joint normal stiffness is therefore significant in rock mechanics.Here,first,seven existing methods for determining joint normal stiffness were introduced and reviewed,among which MethodⅠ(the indirect measurement method),MethodⅡ(the direct determination method),MethodⅢ(the across-joint strain gauge measurement method)and MethodⅣ(the deformation measuring ring method)are via destructive uniaxial compression testing,while MethodⅤ(the best fitting method),MethodⅥ(the rapid evaluation method)and MethodⅦ(the effective modulus method)are through wave propagation principles and nondestructive ultrasonic testing.Subsequently,laboratory tests of intact and jointed sandstone specimens were conducted following the testing requirements and pro-cedures of those seven methods.A comparison among those methods was then performed.The results show that Method I,i.e.the benchmark method,is reliable and stable.MethodⅡhas a conceptual drawback,and its accuracy is acceptable at only very low stress levels.Relative errors in the results from MethodⅢare very large.With MethodⅣ,the testing results are sufficiently accurate despite the strict testing environment and complicated testing procedures.The results from MethodⅤare greatly unstable and significantly dependent on the natural frequency of the transducers.The joint normal stiffness determined with MethodⅥis stable and accurate,although data processing is complex.MethodⅦcould be adopted to determine the joint normal stiffness corresponding to the rock elastic deformation phase only.Consequently,it is suggested that MethodsⅠ,ⅣandⅥshould be adopted for the mea-surement of joint normal stiffness.The findings could be helpful in selecting an appropriate method to determine joint normal stiffness and,hence,to better solve discontinuous rock mass problems.

Digital core based transmitted ultrasonic wave simulation and velocity accuracy analysis

Transmitted ultrasonic wave simulation （TUWS） in a digital core is one of the important elements of digital rock physics and is used to study wave propagation in porous cores and calculate equivalent velocity. When simulating wave propagates in a 3D digital core, two additional layers are attached to its two surfaces vertical to the wave-direction and one planar wave source and two receiver-arrays are properly installed. After source excitation, the two receivers then record incident and transmitted waves of the digital rock. Wave propagating velocity, which is the velocity of the digital core, is computed by the picked peak-time difference between the two recorded waves. To evaluate the accuracy of TUWS, a digital core is fully saturated with gas, oil, and water to calculate the corresponding velocities. The velocities increase with decreasing wave frequencies in the simulation frequency band, and this is considered to be the result of scattering. When the pore fluids are varied from gas to oil and finally to water, the velocity-variation characteristics between the different frequencies are similar, thereby approximately following the variation law of velocities obtained from linear elastic statics simulation （LESS）, although their absolute values are different. However, LESS has been widely used. The results of this paper show that the transmission ultrasonic simulation has high relative precision.

The Optimization of Ultrasonic Extraction Technology of Total Flavonoids in Leaves of Mallotus apelta by Response Surface Analysis Methodology

[Objectives] Response surface analysis methodology was used to optimize the extraction technology of total flavonoids from Mallotus apelta leaves. [Methods] The total flavonoids were extracted using ultrasonic assisted ethanol extraction,and extraction rate of total flavonoids was taken as evaluation index. On the basis of single-factor experiment,Box-Behnken response surface methodology was employed for selecting the optimum extraction process. [Results]The optimum extraction conditions of total flavonoids were as follows: 75% of ethanol concentration,1∶ 25 of ratio of material to liquid,31 min of ultrasonic time. Under these conditions,the extraction rate of total flavonoids was 1. 115%.[Conclusions] The extraction process obtained by response surface methodology was stable,reasonable,accurate and reliable. It was a feasible method to extract the total flavonoids from M. apelta leaves.

Study on Ultrasonic Extraction of Total Flavonoids from Angelica keiskei Koidzumi

[Objective] This study aimed to optimize the ultrasonic extraction process of total flavonoids from Angelica keiskei Koidzumi. [Method] Using the yield of total flavonoids as an indicator, the ultrasonic extraction process of total flavonoids from A. keiskei was optimized by orthogonal experimental design. [ Result ] Among four factors in orthogonal experimental design, extraction time exhibited the most significant effect on the extraction result; ethanol concentration exhib- ited a significant effect on the extraction result; ultrasonic power and solid-to-liquid ratio had no significant effect. The optimal conditions for ultrasonic extraction of total flavonoids from A. keiskei were extraction time of 25 min, ethanol concentration of 80%, ultrasonic power of 60 W, solid-to-liquid ratio of 1：20 （g/ml）, under which the yield of total flavonoids reached 1.56%. [ Conclusion] This study provides the basis for further development and utilization of total flavonoids from A. keiskei.

The Optimization of Ultrasonic Extraction Process of Total Flavonoids from Rhodiola Crenulata by Response Surface Method

[Objectives]To study the optimal extraction process of total flavonoids in Rhodiola rosea L.,and further facilitate the development and utilization of Rhodiola rosea L. [Methods]With Rhodiola crenulata as raw material,ethanol as extractant,ultrasonic extraction as the extraction method,the single factor method was first used for preliminary investigation of effect of ethanol volume fraction,solid-liquid ratio,extraction temperature and extraction time on the flavonoids extraction rate,and then the Box-Behnken response surface method was used to optimize the extraction process of total flavonoids in R. crenulata. [Results] The optimal extraction conditions: ethanol concentration of 72%;solid-liquid ratio of 1∶ 43; extraction temperature of 66℃; extraction time of 50 min. Under these conditions,the extraction rate of total flavonoids from R. crenulata was 2. 591%. [Conclusions] The results showed that the method of response surface was reasonable and feasible for the optimization of ultrasonic extraction of total flavonoids from R. crenulata.

Optimization on Ultrasonic Extraction Method of Total Flavonoids for Liriodendron chinense Sarg.×L.tulipifera L. by Response Surface Methodology (RSM)

With the leaves,bark and roots of Liriodendron chinense Sarg.× L.tulipifera L.as experiment materials,the ultrasonic-assisted extraction conditions of total flavonoids were optimized by response surface Box-Behnken test design.Ultrasonic time and liquid-to-solid ratio had significant effects on the extraction amount of flavonoids from the leaves of L.chinense Sarg.× L.tulipifera L.;ultrasonic temperature and liquid-to-solid ratio had an interaction on the extraction amount of flavonoids;and the optimum ultrasonic time,temperature and liquid-to-solid ratio for the extraction of flavonoids from leaves of L.chinense Sarg.× L.tulipifera L.were 19.82 min,28.60 ℃ and 9.48 ml/g,respectively.Ultrasonic time,temperature and liquid-to-solid ratio had significant effects on the extraction amount of flavonoids from L.chinense Sarg.× L.tulipifera L.;there was a significant interaction between any two of the factors,which had a significant effect on the extraction of flavonoids;and the optimum extraction parameters for flavonoids in the bark were the ultrasonic time of 33.66 min,the ultrasonic temperature at 32.85 ℃ and the liquid-to-solid ratio of 11.39 ml/g.Ultrasonic time and liquid-to-solid ratio had significant effects on the extraction of flavonoids from roots;there was a significant interaction between ultrasonic time and liquid-to-solid ratio and between ultrasonic temperature and liquid-to-solid ratio;and the optimum extraction parameters for flavonoids from the roots of L.chinense Sarg.× L.tulipifera L.were the ultrasonic time of 32.38 min,the ultrasonic temperature at 25.0 ℃ and the liquid-to-solid ratio of 8.00 ml/g.The results of the three models were stable.The models are feasible and have good application value.

Optimization of Ultrasonic Extraction Process of Total Flavonoids from Mulberry Leaves by Orthogonal Experiment

In this study, by using mulberry leaves as raw materials, ultrasonic extraction process of total flavonoids from mulberry leaves was optimized by orthogonal experiment. Results showed that the maximum yield of total flavonoids （6.1% ） was obtained from mulberry leaves with addition of 45 times the volume of water by ultrasonic extraction at 55 ℃ for three times, 55 min each. According to the verification test, the optimized technical process was reasonable and feasible with high stability.

A gas kick early detection method outside riser based on Doppler ultrasonic wave during deepwater drilling

The feasibility of gas kick early detection outside the riser was analyzed based on gas-liquid multiphase flow theory.Then an experimental platform for gas kick early detection based on Doppler ultrasonic wave was established and the propagation experiments in two-phase flow of gas-water(sucrose solutions)were conducted.The time and frequency domains of the Doppler ultrasonic wave signals during the experiments were analyzed.The results show that:(1)No matter the pump was on or off,the detected average Doppler ultrasonic signal voltage increased first and then decreased with the increase of the gas void fraction,and had a quadratic function relation with gas void fraction,so the average voltage change of the monitored signals can be used to deduce the approximate gas void fraction.The Doppler ultrasonic wave signal voltage was significantly reduced in magnitude and variation in the solution with higher viscosity,and the viscosity has stronger impact on the magnitude of signal than density.(2)When the pump was stopped,the Doppler shift increased with the increase of gas void fraction,and the two showed a nearly linear relation,so the detected amount of Doppler shift can reflect the variation of gas void fraction quantitatively.When the pump was on,the sound energy produced by frequency converter had a more significant impact on amplitude spectrum than gas void fraction,so it is impossible to determine whether gas kick occurs by frequency domain signal analysis.(3)This method is a non-contact measurement,with no contact with the drilling fluid and no disruption to the drilling operation.It can quantitatively characterize the gas void fraction according to the change of Doppler ultrasonic signal,enabling earlier detection of gas kick.

Effect of Filler Composition on the Brazing of Alumina to Copper Using Ultrasonic Wave

An ultrasonic wave was applied during brazing of alumina to Cu. First alumina was metallized by applying ultrasonicwave in braze bath. Then the metallized alumina was brazed with Cu using the same filler alloy. The filler used wereZn-Al alloys and Zn-Sn Alloys. The weight percent of Al in filler was ranging between 0, 5%, 10%, respectively.The weight percent of tin in filler was ranging between 0, 30%, 60% and 91%, respectively. The joining mechanismwas investigated by measuring the joining strength, hardness and analyzing the microstructure at interface of thejoint. The shear strength and microstructure of the joint strongly depend on the filler composition. The effect ofultrasound was derived primarily from acoustic cavitations, impact and friction of the filler against alumina ceramic.This improved the wetting between alumina and the filler, and reflected to improve the joint strength. Anotherultrasonic advantage as to reduce of the joining temperature, that reduced the thermal stress in the braze joint.

EFFECT OF ULTRASONIC WAVE ON THE ELECTROPOLYMERIZATION OF PYRROLE

Electropolymerization of pyrrole under ultrasonic field at 20kHz was performed in a series of aqueous and propylene carbonate (PC) solutions. The ultrasonic wave with moderate intensity at the power of 44W, which is the power threshold of the ultrasonic generator used in this work to produce cavitation effect, enhance the conductivity and tensile strength of the polypyrrole films as prepared. However, too high intensity of the ultrasonic wave is harmful to the polymerization.

Non-contact evaluation of the resonant frequency of a microstructure using ultrasonic wave

This paper presents a novel non-contact method for evaluating the resonant frequency of a microstructure, Firstly, the microstructure under test is excited by ultrasonic waves. This excitation method does not impose any undefined load on the specimen like the electrostatic excitation and also this is the first actual use of ultrasonic wave for exciting a microstructure in the literature. Secondly, the amplitudes of the microstructure are determined by image edge detection using a Mexican hat wavelet transform on the vibrating images of the microstructure. The vibrating images are captured by a CCD camera when the microstructure is vibrated by ultrasonic waves at a series of discrete high frequencies （〉30 kHz）. Upon processing the vibrating images, the amplitudes at various excitation frequencies are obtained and an amplitude-frequency spectrum is obtained from which the resonant frequency is subsequently evaluated. A micro silicon structure consisting of a perforated plate （192 × 192 μm） and two cantilever beams （76 × 43 μm） which is about 4 μm thickness is tested. Since laser interferometry is not required, thermal effects on a test object can be avoided. Hence, the setup is relatively simple. Results show that the proposed method is a simple and effective approach for evaluating the dynamic characteristics of microstructures.

Determination of the parameters of a linear-viscoelastic thin layer using the normally-incident ultrasonic waves

This paper proposes a method of simultaneous determination of the four layer parameters （mass density, longitudinal velocity, the thickness and attenuation） of an immersed linear-viscoelastic thin layer by using the normally-incident reflected and transmitted ultrasonic waves. The analytical formula of the layer thickness related to the measured trans- mitted transfer functions is derived. The two determination steps of the four layer parameters are developed, in which acoustic impedance, time-of-flight and attenuation are first determined by the reflected transfer functions. Using the derived formula, it successively calculates and determines the layer thickness, longitudinal velocity and mass density by the measured transmitted transfer functions. According to the two determination steps, a more feasible and simplified measurement setups is described. It is found that only three signals （the reference waves, the reflected and transmitted waves） need to be recorded in the whole measurement for the determination of the four layer parameters. A study of the stability of the determination method against the experimental noises and the error analysis of the four layer parameters are made. This study lays the theoretical foundation of the practical measurement of a linear-viscoelastic thin layer.

Application of Ultrasonic Waves for the Improvement of Water Treatment

Application of ultrasonic waves is one of the novel techniques for the improvement of water treatment process. In this study, ultrasonic waves were irradiated to drinking water for water softening process and other contaminants removal. The experimental results showed that this technique improved the water treatment process efficiently. The study also revealed that the various parameters such as amplitude, frequency and irradiation time could affect the efficiency of ultrasound techniques for the improvement of water quality.

Mass transfer enhancement for LiBr solution using ultrasonic wave

The methods were studied to improve the cooling performance of the absorption refrigeration system(ARS) driven by low-grade solar energy with ultrasonic wave, while the mechanism of ultrasonic wave strengthening boiling mass transfer in LiB r solution was also analyzed with experiment. The experimental results indicate that, under the driving heat source of 60–100 oC and the ultrasonic power of 20–60 W, the mass flux of cryogen water in Li Br solution is higher after the application of ultrasonic wave than auxiliary heating with electric rod of the same power, so the ultrasonic application effectively enhances the heat utilization efficiency. The distance H from ultrasonic transducer to vapor/liquid interface significantly affects mass transfer enhancement, so an optimal Hopt corresponding to certain ultrasonic power is beneficial to reaching the best strengthening effect for ultrasonic mass transfer. When the ultrasonic power increases, the mass transfer obviously speeds up in the cryogen water; however, as the power increases to a certain extent, the flux reaches a plateau without obvious increment. Moreover, the ultrasound-enhanced mass transfer technology can reduce the minimum temperature of driving heat source required by ARS and promote the application of solar energy during absorption refrigeration.

3D attitude measurement by means of spread spectrum modulated ultrasonic wave

Local position and attitude measurement methods are necessary for the navigation of autonomous robots and the other applications. In this paper, a 3D attitude measurement method is proposed. This method uses the spread spectrum modulated ultrasonic wave for the measurement of the pseudo-distance of each pathway between transmitter and receiver elements that are arranged in an array. The relative attitude between transmitter and receiver unit could be calculated from the relationship between these pseudo-distances. The use of spread spectrum modulation enables the high accuracy, noise tolerance, and coexistence of multiple transmitter devices by code division multiple access (CDMA) between transmitter and receiver elements. A pair of transmitter and receiver prototype for proposed method was manufactured, and the fundamental performance was examined in experiments. As a result, accuracy of σ=0.78 mm in the pseudo-distance measurement of each pathway between transmitter and receiver elements was confirmed, and about 0.01 rad resolution in the attitude measurement was also confirmed.

High Pressure-Differential Thermal Analysis and Ultrasonic Wave Amplitude Analysis of Ice-Water Equilibrium at 1.5-5.0 GPa

Water is the most active component in all geological systems. It has an importanteffect on the physical properties of minerals and melts. It also plays a key role in the evolutionof the Earth. Accurate thermodynamics data on water are currently confined to pressures below1.0 GPa and temperatures below 900℃. Presented in this paper are new data available on theP-T properties of water at pressures up t0 5. 0 GPa, developed from differential thermal analysis and ultrasonic wave amplitude analysis. It has been found that there may exist anotherternary point at 3. 0 GPa and that ultrasonic wave amplitude change of ice-water transitionshows two inflection points above 2. 0 GPa, consistent with the two peaks of differential thermal curves above 2. 0 GPa. It may be a new phenomenon which needs further study.

Improving performance of direct coal liquefaction through swelling with solvent under the radiation of ultrasonic wave

Three kinds of lower rank bituminous coals from Yanzhou mine and Tengxian mine from Shandong Province were treated and hydrogenated in the study. The test re- sults show that the performance of hydrogenation liquefaction of the pretreated coals is improved markedly. Under the test condition of H2 initial pressure 8.2 MPa, addition of FeSO4·7H2O and S as catalyst, final reacting temperature 400 ℃ and reacting time 1.5 h, the oil yield of pretreated YZ1 coal is 69.76% compared with 62.53% of oil yield of un- treated YZ1. Seminally the oil yield of pretreated YZ2 coal is 55.43% compared with 20.88% of untreated YZ2 coal. The results of tests also prove that the improving degree of hydrogenation liquefaction of the pretreated coals is related with radiation duration when the radiation frequency and radiation power of ultrasonic wave are fixed.

Damage Detection in CFST Column by Simulation of Ultrasonic Waves Using STFT-Based Spectrogram and Welch Power Spectral Density Estimate

Structural health monitoring employs different tools and techniques to provide a prediction for damages that occur in various structures.Damages such as debond and cracks in concrete-filled steel tube column(CFST)are serious defects that threaten the integrity of the structural members.Ultrasonic waves monitoring applied to the CFST column is necessary to detect damages and quantify their size.However,without appropriate signal processing tools,the results of the monitoring process could not be crucial.In this research,a monitoring process based on a Multiphysics numerical simulation study was carried out.Two signal processing tools:short time Fourier transform(STFT)and Welch Power Spectral Density Estimate(PSD)were used to analyse the captured raw signals.The STFT spectrogram was effective in identifying the different size of damage based on a graphical interpretation.The results show that the increasing of frequency of the excited signal give a better results.The increase in peak magnitude values in Welch PSD was found to be proportionate to the change in damage length whereas the damage depth has a less effect.The results for the crack size identification were less promising than those of debond damage because of the different type of the signal’s propagation path.Simulation process conducted by COMSOL software has proved the validity of the adopted signal processing techniques in detecting such damages in CFST columns.