New anthraquinone derivatives from the roots of Berchemia floribunda

A new naphthoquinone-anthraquinone dimer named floribundiquinone E （1） and a new anthraquinone 2-acetylphyscion （2） were isolated from the roots of Berchemia floribunda Brongn. Their structures were elucidated on the basis of spectroscopic methods.

Studies on the mechanisms of anti-inflammatory activity of the extracts and fractions of Alchornea floribunda leaves

Objective:Alchornea floribunda leaves are widely used in ethnomedicinal management of inflammatory disorders. The present work is aimed at investigating this folkloric use.Methods:The anti-inflammatory effect of the leaf extracts and fractions was investigated in experimental animal models of acute and chronic inflammation. The possible mechanisms by which the two most active fractions,hexane(HE) and ethyl acetate(EF) exert their effects were also investigated.Results:The crude extract(200 mg/kg) showed moderate inhibition of egg albumen-induced edema in rats(%edema inhibition = 54.69) at 4 h.HE and EF showed very high activity(%edema inhibition of 81.25 and 67.19 respectively at 200 mg/kg) at 4h as compared to the control. Both fractions ameliorated arthritis induced by formaldehyde in rats.At 400 mg/kg,HE evofeed a significant irritation of gastric mucosa in rats.EF(200 mg/kg,p.o.) significantly inhibited leucocytes(%inhibition = 36.79) migration in vivo,but could not stabilize heat and hypotonicity-indueed lysis of human erythrocyte at 200 and 400μg/mL in vitro.Phytochemical investigation revealed the presence of terpenoids and steroids in HE and flavonoids,tannins and saponins in EF.Conclusion:These results suggest that the leaves of Alchornea floribunda possess anti-inflammatory activity in acute and chronic inflammation.The activity may derive from a combination of inhibition of prostaglandin synthesis and leucocytes migration.The phytochemical constituents detected in HE and EF may account for the anti-inflammatory activity.

Study on Cell Suspension Culture of Floribunda Rose

Friable callus was induced when immature seeds of floribunda rose were inoculated on MS medium supplemented with 2,4-D 3.0 mg.L^-1. When transfered onto subculture media, friable callus developed into embryogenic callus, which was used to establish cell suspension lines. Cell suspensions had to be subcultured at a interval of 4-5 days at the first several culture cycles. The best subculturing cycle for the stable cell suspensions was 8-10 days. The best inoculum quantity was 1 mL PCV（Packed Cell Volume） per 40 mL culture fluid.

Comparative Test on Adaptability of Different Floribunda Rose Cultivars in Sanya Area

[Objective] This study aimed to screen out floribunda rose cultivars resistant to high temperature in tropical areas. [Method] A total of 8 floribunda rose cul- tivars （Carefree Wonder, Scloss Mannheim, Bright Smile, Goldmarie, Betty Prior, Red Riding Hood Fairy Tale, Golden Scepter and Iceberg） were selected as the research objects, and their growth, adaptability and disease resistance in Sanya area were compared. [Result] Carefree Wonder, Scloss Mannheim, Iceberg and Bright Smile were suitable to be planted in Sanya area. Among them, Carefree Wonder, Scloss Mannheim and Iceberg were characterized with fewer diseases, bright colors, heat resistance and strong stress resistance, and they could be planted as ornamental flowers in Sanya area. [Conclusion] This study will provide a reference for the selection of ornamental flowers in Sanya area.

Bredemeyera floribunda Wild Elemental Content and Comparison with Nutritional Recommendation for Adults:Medicinal Plant Used by the Brazilian Rural Population to Treat Fatigue and Exhaustion

Bredemeyera floribunda Wild (Polygalaceae), also known as pau-de-ovo is a medicinal plant used to treat snakebites, as tonic and fortifiers to treat fatigue and exhaustion. The aim of the present study was to document the knowledge of herbal sellers in the preparation of the so-called “B. floribunda fortifier” and to compare the plant root content with the recommended nutritional values and the permitted limit for medicinal plants. The study revealed that, unlike other regions of Brazil, in the Midwest region, the root of the plant is used as food to treat fatigue and exhaustion. The contents in B. floribunda root decreased in the following order: Ca > K > P > Na > Fe > Mg > Si > Mn > Zn > Cu. Daily intake of 30 grams of B. floribunda roots are considered as an excellent source of Cr, Cu, Mn and Se for people 19 to 50 years of age according to the RDA. In addition, Cu and Fe showed content above the established limit for medicinal plants stipulated by other countries. The root of the plant has low K, Mg, P, Na and Zn content. When the contents in roots of the plant are below the oral dose of reference, consumption of this plant can be considered safe and without risk to human health. On the other hand, concentration of elements such as Cu, Fe, Ni, Al, Cr and Se exceeding the allowable limit may cause serious health problems due to prolonged ingestion and large amounts consumed daily. The Brazilian legislation does not establish a permissible limit of macro- and micro-elements in medicinal plants;thus, the results presented in this study will contribute to the Brazilian public health policy on medicinal plants.

基质和外源激素处理对多花勾儿茶高空压条生根的影响

多花勾儿茶是我国集蜜源、观赏和药用等功能为一体的传统珍贵树种,具有较好的生态和经济效益。为优选其高空压条繁殖的最佳条件,提高和完善其无性快繁技术,采用四因素三水平L9(3~4)正交试验设计,探究不同基质配比、外源激素种类、外源激素浓度以及环剥宽度对多花勾儿茶高空压条生根的影响,并运用模糊数学隶属函数法和相关性分析法对各项生根指标进行综合评价,探讨4种因素对压条生根的作用机制。结果显示,适宜的基质和外源激素配比可显著促进多花勾儿茶形成愈伤组织并生根,同时可使各项根部指标增加。方差分析和极差分析表明,生根效果存在基质、外源激素和环剥的差异,其中基质配比和外源激素种类是影响各生根指标的最主要因素。综合评价得出,最有利于多花勾儿茶压条生根的条件为:珍珠岩+草炭+蛭石(1∶1∶1)作为基质、NAA作为生长调节剂、NAA浓度为400 mg/L,环剥宽度为10 mm,该条件下愈伤组织诱导率高达(93.33±2.36)%,生根率高达(85.00±4.08)%,(9.67±0.47)d即可在伤口处形成愈伤组织,(14.00±0.82)d开始生根,平均一级根数[(15.44±0.51)根]、平均一级根长[(7.13±0.25)cm]、平均一级根粗[(3.03±0.21)mm]、根鲜质量[(3.29±0.05)g]、根干质量[(0.67±0.04)g]、根系效果指数(5.50±0.06)、平均隶属函数值(0.77)均为最高。该研究可为建立多花勾儿茶快繁体系,推广多花勾儿茶栽培提供理论参考和数据支撑。

萼翅藤叶主要活性成分的超声波提取工艺优化

文章通过采用超声波辅助技术提取萼翅藤叶片的活性成分,探究提取时间、料液比、超声功率、提取温度4个因素对多糖、黄酮、多酚3种主要活性成分提取量的影响,并设计正交试验优化提取工艺,确定最佳提取工艺条件为:提取时间40 min,料液比1∶50 g/m L,超声功率600 W,提取温度为15℃。在此条件下,多糖、黄酮、多酚提取量分别为32.65 mg/g、3.73 mg/g、62.35 mg/g。结果表明,超声波提取技术可以作为一种高效绿色的方法来提取萼翅藤的活性成分,为萼翅藤的开发及绿色应用提供参考。

南阳市环保丰花月季引种栽培比较试验

环保丰花月季既有美丽的花色、较好的花型,又具有抗逆性强、花期长、花量大等优良特性。本试验从江苏沭阳引进10个丰花月季品种,对其植株生长发育情况、花部观赏性和抗逆性等多项指标进行观测和比较分析。结果表明,约定、红色重瓣绝代佳人、遥远的鼓声、阳光玫迪兰和樱桃伯尼卡5个品种具有花量大、盛花期持续时间长、花朵自洁性好、耐热抗寒耐涝能力强、对月季三大病虫害几乎免疫等优良特性,适宜在南阳市园林绿化、景观配置中栽培推广应用。

血清WFA+-M2BP、DNASE1L3联合检测对乙型肝炎病毒相关性肝癌患者肝切除术后复发的预测价值

目的探讨乙型肝炎病毒相关性肝癌(HBV-HCC)患者肝切除术后两年内复发的影响因素,分析血清紫藤花凝集素阳性人Mac-2结合蛋白(WFA^(+)-M2BP)联合脱氧核糖核酸酶1类似物3(DNASE1L3)检测用于评估术后复发的价值。方法选择2017年1月至2019年11月宜昌市第一人民医院收治的HBV-HCC并接受肝切除术的患者122例作为研究对象,根据随访结果,分为复发组(42例)、未复发组(80例)。检测两组患者术前的血清WFA^(+)-M2BP、DNASE1L3水平,对影响术后复发的因素进行多因素Logistic回归分析,使用受试者工作特征(ROC)曲线分析WFA^(+)-M2BP、DNASE1L3水平对术后两年内复发的评估价值。结果复发组的血清WFA^(+)-M2BP水平明显高于未复发组,DNASE1L3水平明显低于未复发组(P<0.05);单因素分析结果中,患者有门静脉侵犯、肿瘤最大径、WFA^(+)-M2BP、DNASE1L3、甲胎蛋白(AFP)水平、肝癌分期及肿瘤分化程度是影响术后复发的独立相关因素(P<0.05);多因素Logistic回归分析中,有门静脉侵犯、肿瘤最大径≥2 cm、WFA^(+)-M2BP≥4.74 COI是影响术后复发的危险因素(P<0.05,OR>1),而DNASE1L3≥1.10 ng/L是影响术后复发的独立保护因素(P<0.05,OR<1)。ROC曲线分析显示,WFA^(+)-M2BP和DNASE1L3的联合检测对预测HBV-HCC术后复发有较高的价值,其曲线下面积为0.890(95%CI:0.835~0.952)。结论血清WFA^(+)-M2BP、DNASE1L3是HBV-HCC术后两年内复发的影响因素。此外,患者的血清WFA^(+)-M2BP、DNASE1L3水平变化对HBV-HCC患者术后两年内复发均具有较好的预测价值,二者联合检测具有更高的预测效能。

香山公园常见地被植物生长特性及根系研究

在古树下种植乡土地被植物,既可保护古树下土地免受践踏,又有利于改善土壤的透气和保水能力。但栽植前需掌握地被植物的根系生长特性,评估其对古树根系的影响。利用Epson数字扫描仪,配合直接测定法,对香山公园内4种常见地被植物(多花胡枝子、菊花脑、青绿薹草和求米草)在古树油松、古树白皮松及苗圃3种立地条件下的根系及地上部生长情况进行分析。苗圃条件下的相同年份多花胡枝子植株,根系及地上部发育均显著优于白皮松及油松下植株;菊花脑在3种立地条件下均表现良好,根系及地上部发育未表现出显著差异;在相同立地条件下的求米草根系及地上部发育显著优于菊花脑和青绿薹草,且不同立地条件下的求米草地上部分和根系发育差异较小。研究结果显示,菊花脑和求米草在不同立地条件下均表现良好,且不易对古树根系产生影响,适宜在古树下栽植;多花胡枝子适宜在立地环境较陡的古树下栽植;青绿薹草不适于作为古树林下地被使用。

野生花卉多花胡枝子种子萌发特性

Germination of Lespedeza floribunda Bunge.seeds was studied in order to provide cultivation and application in gardening and landscaping.Results indicated that the seeds of Lespedeza floribunda had the characteristic of forcing dormancy that was caused by hard seed vessels.The dormancy could be released by soaking in hot water and by sulphuric acid treatment.The most appropriate treatment time was 10～30 min,resulting in average germination rate(96%),average germination power(82.53%)and average germination index(17.54).The optimum germination temperature was 25 ℃ and the germination rate,germination power and germination index was 95%,81%,22.95 respectively after treated by sulphuric acid for 20 min.

多花含笑叶的挥发油成分研究

运用气相色谱 质谱 计算机联用技术 ,结合标准谱库 ,对昆明产多花含笑叶的挥发油成分进行了研究 ,分离鉴定了 42个化合物 ,其中单萜 16个 ,倍半萜 2 1个 ,分别占精油总含量的 2 1 2 1%和49 0 8%。主要成分为γ 木罗烯 (12 0 4% ) ,十六碳三烯酸甲酯 (7 45 % ) ,兰桉醇 (7 38% ) ,1,8 桉叶素(5 76 % ) ,α 愈创木烯 (5 6 4% ) ,香桧烯 (4 6 2 % ) ,十六碳二烯酸甲酯 (4 2 6 % ) ,β 榄香烯 (3 76 % ) ,β 蒎烯 (3 2 0 % )

多花胡枝子基因组DNA提取与RAPD反应体系优化

以多花胡枝子干种子、萌发种子及开花期叶片三种材料提取基因组DNA,其中开花期叶片用CTAB法提取、干种子和萌发种子用SDS法提取的DNA均用于RAPD研究。结果表明,叶片基因组DNA的RAPD扩增最佳反应体系是:DNA模板用量为75ng,Mg2+浓度2.1mmol/L,dNTP浓度200μmol/L,随机引物浓度为0.2pm/μL,10×buffer2.5uL,TaqDNA聚合酶1.5个单位,反应总体积25uL;扩增反应程序:94℃预变性3min;94℃变性30s,36℃退火30s,72℃延伸1min,循环45次,72℃延伸10min。

大孔树脂纯化萼翅藤总黄酮工艺

筛选纯化萼翅藤总黄酮的最佳树脂,并对影响这种树脂分离的主要因素进行研究,确定大孔树脂纯化总黄酮的最佳工艺参数。选择4种大孔树脂分别对萼翅藤总黄酮进行静态与动态吸附性能研究,考察影响分离的各种因素如上样液pH值、上样液质量浓度、上样量和洗脱剂体积分数等。HPD-450树脂分离效果最好,Langmuir等温吸附模型较Freundlich模型更适宜描述树脂对萼翅藤总黄酮的吸附,其吸附分离萼翅藤总黄酮的最佳工艺为:上样液pH值为5,质量浓度为0.6—0.8 mg/mL,上样量为3 BV(树脂床体积),洗脱液为体积分数70%的乙醇,洗脱剂用量为3 BV。经HPD-450树脂吸附分离后,总固物中总黄酮质量分数从20.23%提高到75.86%,纯度提高了3倍多,回收率为62.65%。

娃儿藤抗肿瘤活性部位的成分

目的:研究娃儿藤属植物七层楼根中的抗肿瘤活性成分。方法:在抗肿瘤活性部位筛选的基础上,利用多种柱色谱方法对其中的化学成分进行了分离,通过理化常数和波谱分析鉴定了所得化合物的结构。结果:从该植物中共分得8个化合物,其结构分别鉴定为:异去羟基娃儿藤宁(antofine,Ⅰ)、新白前酮(hancolu-penone,Ⅱ)、新白前醇(hancolupenol,Ⅲ)、香荚兰酸(vanillicacid,Ⅳ)、丁香酸(syringicacid,Ⅴ)、阿魏酸(ferulicacid,Ⅵ)、β-谷甾醇(β-sitosterol,Ⅶ)、胡萝卜苷(daucosterol,Ⅷ)。结论:此8个化合物均为首次从该植物中分到,其中Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ均为首次从该属植物中分到,Ⅰ有明显抑制肿瘤细胞生长的作用。

江西南昌引种丰花及微型月季品种的鉴定评估

利用灰色关联度分析和模糊综合评判分析对新引进的6个丰花月季和7个微型月季品种进行了综合分析评判,结果表明:丰花月季品种中北京红和紫色美地兰综合表现最好,其次为皇家巴西诺和仙境,金玛丽表现最差;微型月季品种中古川和梅郎珍珠综合表现最好,紫色时代、和谐和红宝石表现也较好,太阳姑娘表现最差;两种综合评估方法的分析结果基本一致,且与品种的实际表现相一致。

丰花月季扦插繁殖技术研究

用茎 (枝 )插法研究了 2个丰花月季品种曼海姆 (Sohlossmannieim)和马蒂尔德 (Matilda)的扦插繁殖技术。结果表明 :对枝条进行环剥处理 ,只要技术熟练 ,不用任何激素 ,扦插成活率可达 90 %以上 ;用嫩枝和硬枝扦插时 ,将插穗基部用浓度为 80 0～ 10 0 0ng/L的ABT生根剂浸泡 5min ,可提高丰花月季的成活率 ,且获得的扦插苗质量较好。

电阻抗断层成像评价丰花月季抗寒性

【目的】研究电阻抗断层成像(electrical impedance tomography,EIT)技术评价丰花月季抗寒性的可行性,为快速、非破坏性地检测观赏植物抗寒性提供新的可借鉴方法。【方法】以3个多年生丰花月季(Rosa hybrida Hort.‘Floribunda’)品种‘红帽’(Hongmao)、‘柔情似水’(Rouqingsishui)、‘仙境’(Xianjing)为试材,测定当年生茎段在自然低温以及人工冷冻处理下EIT图像和电阻抗图谱(electrical impedance spectroscopy,EIS)参数的变化,并与传统电导法(electrolyte leakage,EL)测定的抗寒性进行相关分析。同时,对自然低温下3个丰花月季品种茎的胞外电阻率r_e、胞内电阻率r_i、弛豫时间t和弛豫时间分布系数ψ4个EIS参数与EIT重构值进行相关性分析。【结果】3个丰花月季品种在最冷月抗寒性顺序为:‘仙境’>‘柔情似水’>‘红帽’;各个品种不同温度下的茎段都可以得到清晰的EIT图像,且EIT重构值随温度降低呈减小的趋势,通过EIT重构值计算的抗寒性与电导法求得的抗寒性有极显著的相关性(r=0.92),但较EL法测得的抗寒性低。抗寒锻炼期间,EIS参数均发生变化,总体上,胞外电阻率r_e呈上升趋势,弛豫时间t呈下降的趋势;自然低温下,‘红帽’和‘仙境’的胞外电阻率r_e与EIT重构值正相关(r>0.72),‘柔情似水’和‘仙境’的弛豫时间t同样和EIT重构值正相关(r>0.63),说明EIT重构值、r_e和t可以表征细胞、组织的状态。【结论】冷冻处理下,丰花月季枝条受到冻害以后,EIT图像重构值显著降低,可以通过重构值用Logistic方程拟合计算,得到抗寒性,但是所得抗寒性较EL法低。未经冷冻处理的胞外电阻率r_e和弛豫时间t两个EIS参数与EIT重构值有一定的相关性。样本经冷冻处理后,可以用茎的电阻抗断层成像(EIT)图像重构值评价丰花月季的抗寒性。

商洛多花胡枝子根瘤菌16S rDNA-RFLP分析及系统发育研究

利用16S rDNA-RFLP和全序列测定方法,对分离自商洛地区5个分布点的59株多花胡枝子根瘤菌进行了RFLP分析和系统发育研究.结果表明:(1)42株供试菌株归属根瘤菌属(Rhizobium)、11株归属中华根瘤菌属(Sinorhizobium).其余6株非根瘤菌中3株是嗜麦芽黄单胞菌(Stenotrophomonas maltophilia)、3株是解淀粉类芽孢杆菌(Paenibacillus amylolyticus),说明胡枝子根瘤内生菌较为丰富且类型多样.(2)结合供试菌株的地理生境分析,发现来自不同采集点的菌株有些具有同样的遗传类型,而来自同一采集点的菌株遗传类型却有差异,证明胡枝子根瘤菌在分群类别上与地理环境之间没有明确的对应关系,地理环境并非根瘤菌多样性形成的主要因素.建议今后对根瘤菌多样性研究应从根瘤菌与寄主植物物之间的共生选择进化,特别是对共生体系中基因的横向转移方面进行深入探讨.