Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the presen...Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the present study,Illumina's second-generation sequencing technique was used to determine the variation in gene expression at the transcriptome level in western flower thrip before and after infection with INSV. By aligning 168 188 unigenes of western flower thrip to the NCBI protein database,47 558 unigenes were annotated,accounting for 28. 27% of the total. Species classification revealed that 58. 7% of the annotated unigenes of western flower thrip did not match any other species,and 15. 4% of had a match in Zootermopsis navadensis,12. 4% in Mus musculus,7. 3% in Mustela putorius,3. 4% in Theileria parva,and 2. 7% in Tribolium castaneum. By analyzing DEGs between INSV-free and INSV-carrying western flower thrips,we found that 11 genes were upregulated,and 9 genes were downregulated in western flower thrip after INSV infection. In addition,cell cycle and ubiquitin-proteasome pathway in western flower thrip were significantly infected by INSV infection.展开更多
Flower thrips are one of the key pests in production of southern highbush blueberries (Vaccinium corymbosum L. × V. darrowi Camp). They feed on the reproductive parts of the flower and reduce yield. Very few inse...Flower thrips are one of the key pests in production of southern highbush blueberries (Vaccinium corymbosum L. × V. darrowi Camp). They feed on the reproductive parts of the flower and reduce yield. Very few insecticides are available to manage thrips population. Therefore, it is necessary to evaluate pre-existing and experimental insecticides for their efficacy against thrips. There is also a high demand for reduced-risk insecticides that are less toxic to beneficial insects and the environment. In this study, a number of conventional and reduced-risk insecticides were evaluated against flower thrips on farms in Florida and Georgia and in the laboratory. The best performing insecticides were also evaluated in the laboratory for their effects on Orius insidiosus Say, which is a common thrips’ predator in Florida. Assail 70WP (Acetamiprid) and SpinTor 2SC (Spinosad) were the most promising insecticides in reducing thrips numbers in the field as well as in laboratory experiments. SpinTor 2SC killed all of the thrips in laboratory arenas within 4 hours of application and showed low toxicity toward O. insidiosus. All of the compounds tested performed as well as the conventional pesticide malathion.展开更多
基金Supported by National Natural Science Foundation of China(31301665)
文摘Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the present study,Illumina's second-generation sequencing technique was used to determine the variation in gene expression at the transcriptome level in western flower thrip before and after infection with INSV. By aligning 168 188 unigenes of western flower thrip to the NCBI protein database,47 558 unigenes were annotated,accounting for 28. 27% of the total. Species classification revealed that 58. 7% of the annotated unigenes of western flower thrip did not match any other species,and 15. 4% of had a match in Zootermopsis navadensis,12. 4% in Mus musculus,7. 3% in Mustela putorius,3. 4% in Theileria parva,and 2. 7% in Tribolium castaneum. By analyzing DEGs between INSV-free and INSV-carrying western flower thrips,we found that 11 genes were upregulated,and 9 genes were downregulated in western flower thrip after INSV infection. In addition,cell cycle and ubiquitin-proteasome pathway in western flower thrip were significantly infected by INSV infection.
文摘Flower thrips are one of the key pests in production of southern highbush blueberries (Vaccinium corymbosum L. × V. darrowi Camp). They feed on the reproductive parts of the flower and reduce yield. Very few insecticides are available to manage thrips population. Therefore, it is necessary to evaluate pre-existing and experimental insecticides for their efficacy against thrips. There is also a high demand for reduced-risk insecticides that are less toxic to beneficial insects and the environment. In this study, a number of conventional and reduced-risk insecticides were evaluated against flower thrips on farms in Florida and Georgia and in the laboratory. The best performing insecticides were also evaluated in the laboratory for their effects on Orius insidiosus Say, which is a common thrips’ predator in Florida. Assail 70WP (Acetamiprid) and SpinTor 2SC (Spinosad) were the most promising insecticides in reducing thrips numbers in the field as well as in laboratory experiments. SpinTor 2SC killed all of the thrips in laboratory arenas within 4 hours of application and showed low toxicity toward O. insidiosus. All of the compounds tested performed as well as the conventional pesticide malathion.
