In this study, an optimized high performance liquid chromatography-fluorescence detector (HPLC-FL) method for the determination of benzo[a]pyrene in edible oil was established. HPLC was performed with Thermo Fisher Sc...In this study, an optimized high performance liquid chromatography-fluorescence detector (HPLC-FL) method for the determination of benzo[a]pyrene in edible oil was established. HPLC was performed with Thermo Fisher Scientific C18 column (250 mm×4.6 mm, 5 μm) as the chromatographic column and acetonitrile and water as the mobile phase, and the excitation wavelength and emission wavelength of fluorescence detector were 286 and 430 nm, respectively. The response was high, and the linear range was 0.5-10.0 ng/ml. The lowest limit of detection was 0.11 ng/ml, and the average recovery was 92.5%. This method is suitable for quantitative analysis of benzo[a]pyrene content in edible oil.展开更多
Sulfur dioxide and its derivative sulfite widely existed in air,water as the environment pollutant.Sulfite is also commonly used as preservative and additive in fresh fruits,vegetables,wines and pharmaceutical materia...Sulfur dioxide and its derivative sulfite widely existed in air,water as the environment pollutant.Sulfite is also commonly used as preservative and additive in fresh fruits,vegetables,wines and pharmaceutical materials.Due to sulfite is closely related with human diseases,it is very urgent for the sensitive and rapid quantification of sulfite in various samples.In our study,a turn-on near infrared(NIR)fluorescent probe(MDQ)was developed for sulfite detection based on a Michael addition reaction,with high sensitivity(LOD 4.16 nmol/L),selectivity and fast response time(400 s).Using MDQ,a quantify method for sulfite in traditional Chinese medicines(TCMs)was developed with the advantages of high precision,accuracy and convenient operation.Furthermore,according to the photophysical property of MDQ,a portable fluorescence detector is designed to quantify sulfite for TCMs and surface water in Dalian city of China.Therefore,the developed fluorescent probe MDQ and portable fluorescent detector as a rapid inspection instrument were successfully used to real-time monitor the sulfite in various complex samples.展开更多
JCT-2000 micro-computer-controlled universal fluorescent magnetic flaw detector, made by the Tianjin No.6 Machine Tool Plant, is used to detect flaws and fatigued cracks from forged products,machined products and
[ Objective] To develop an efficient and practical method for detecting avermectin residue in animal-derived food. [ Method ] Acetonitrile was used as the extraction fluid. After the removal of moisture, trifluoroacet...[ Objective] To develop an efficient and practical method for detecting avermectin residue in animal-derived food. [ Method ] Acetonitrile was used as the extraction fluid. After the removal of moisture, trifluoroacetic anhydride and N-methyl imidazole were used for derivatization and mixed well. After complete derivatization, high-performance liquid chromatography （HPLC） with C18 column was used for accurate qualitative and quantitative analysis of the detected substance using methanol aqueous solution （92： 8, V/ V）. [ Result] The detection limit of HPLC connected with a fluorescence detector was up to 0.1 ug/L. When the standard was added to the substrate at a final concentration in the range of 0.1 -20.0 lag/L, the recoveries were in the range of 80% -108% and the relative deviation was in the range of 4.1% -11.4%. [ Conclusion] The developed HPLC con- nected with a fluorescence detector is simple, practical, highly sensitive and resistant to interference, and it thus can meet the needs for determina- tion of biological toxin residues of avermectins in animal-derived food in laboratory.展开更多
文摘In this study, an optimized high performance liquid chromatography-fluorescence detector (HPLC-FL) method for the determination of benzo[a]pyrene in edible oil was established. HPLC was performed with Thermo Fisher Scientific C18 column (250 mm×4.6 mm, 5 μm) as the chromatographic column and acetonitrile and water as the mobile phase, and the excitation wavelength and emission wavelength of fluorescence detector were 286 and 430 nm, respectively. The response was high, and the linear range was 0.5-10.0 ng/ml. The lowest limit of detection was 0.11 ng/ml, and the average recovery was 92.5%. This method is suitable for quantitative analysis of benzo[a]pyrene content in edible oil.
基金supported financially by Distinguished Professor Program of Liaoning Province(No.XLYC2002008)Dalian Science and Technology Leading Talents Project(Nos.2019RD15,2020RJ09 and 2020RQ076)+2 种基金Liaoning Provincial Natural Science Foundation(No.20180550761)Liaoning Revitalization Talents Program(No.XLYC1907017)the Open Research Fund of the School of Chemistry and Chemical Engineering,Henan Normal University(No.2021YB07).
文摘Sulfur dioxide and its derivative sulfite widely existed in air,water as the environment pollutant.Sulfite is also commonly used as preservative and additive in fresh fruits,vegetables,wines and pharmaceutical materials.Due to sulfite is closely related with human diseases,it is very urgent for the sensitive and rapid quantification of sulfite in various samples.In our study,a turn-on near infrared(NIR)fluorescent probe(MDQ)was developed for sulfite detection based on a Michael addition reaction,with high sensitivity(LOD 4.16 nmol/L),selectivity and fast response time(400 s).Using MDQ,a quantify method for sulfite in traditional Chinese medicines(TCMs)was developed with the advantages of high precision,accuracy and convenient operation.Furthermore,according to the photophysical property of MDQ,a portable fluorescence detector is designed to quantify sulfite for TCMs and surface water in Dalian city of China.Therefore,the developed fluorescent probe MDQ and portable fluorescent detector as a rapid inspection instrument were successfully used to real-time monitor the sulfite in various complex samples.
文摘JCT-2000 micro-computer-controlled universal fluorescent magnetic flaw detector, made by the Tianjin No.6 Machine Tool Plant, is used to detect flaws and fatigued cracks from forged products,machined products and
文摘[ Objective] To develop an efficient and practical method for detecting avermectin residue in animal-derived food. [ Method ] Acetonitrile was used as the extraction fluid. After the removal of moisture, trifluoroacetic anhydride and N-methyl imidazole were used for derivatization and mixed well. After complete derivatization, high-performance liquid chromatography （HPLC） with C18 column was used for accurate qualitative and quantitative analysis of the detected substance using methanol aqueous solution （92： 8, V/ V）. [ Result] The detection limit of HPLC connected with a fluorescence detector was up to 0.1 ug/L. When the standard was added to the substrate at a final concentration in the range of 0.1 -20.0 lag/L, the recoveries were in the range of 80% -108% and the relative deviation was in the range of 4.1% -11.4%. [ Conclusion] The developed HPLC con- nected with a fluorescence detector is simple, practical, highly sensitive and resistant to interference, and it thus can meet the needs for determina- tion of biological toxin residues of avermectins in animal-derived food in laboratory.
