Several studies have investigated the protective functions of brain-derived neurotrophic factor（BDNF） in retinitis pigmentosa. However, a BDNF-based therapy for retinitis pigmentosa is not yet available. To develop ...Several studies have investigated the protective functions of brain-derived neurotrophic factor（BDNF） in retinitis pigmentosa. However, a BDNF-based therapy for retinitis pigmentosa is not yet available. To develop an efficient treatment for fundus disease, an eukaryotic expression plasmid was generated and used to transfect human 293 T cells to assess the expression and bioactivity of BDNF on acute retinal pigment epithelial-19（ARPE-19） cells, a human retinal epithelial cell line. After 96 hours of co-culture in a Transwell chamber, ARPE-19 cells exposed to BDNF secreted by 293 T cells were more viable than ARPE-19 cells not exposed to secreted BDNF. Western blot assay showed that Bax levels were downregulated and that Bcl-2 levels were upregulated in human ARPE-19 cells exposed to BDNF. Furthermore, 293 T cells transfected with the BDNF gene steadily secreted the protein. The powerful anti-apoptotic function of this BDNF may be useful for the treatment of retinitis pigmentosa and other retinal degenerative diseases.展开更多
Green fluorescent protein（GFP） plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore（BHQ） for controlling its expression with exact spatiotemporal resolution.In vitro and in vivo experiments clearly verifie...Green fluorescent protein（GFP） plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore（BHQ） for controlling its expression with exact spatiotemporal resolution.In vitro and in vivo experiments clearly verified that,comparing with Bhc caging, the expression level of caged GFP plasmid was dramatically decreased and then efficiently restored after subsequent photolysis.展开更多
A plasmid transfer-mediated bioaugmentation method for the enhancement of dichlorodiphenyltrichloroethane（DDT） degradation in soil was developed using the catabolic plasmid pDOD from Sphingobacterium sp. D-6. The p ...A plasmid transfer-mediated bioaugmentation method for the enhancement of dichlorodiphenyltrichloroethane（DDT） degradation in soil was developed using the catabolic plasmid pDOD from Sphingobacterium sp. D-6. The p DOD plasmid could be transferred to soil bacteria, such as members of Cellulomonas, to form DDT degraders and thus accelerate DDT degradation. The transfer efficiency of pDOD was affected by the donor, temperature,moisture, and soil type. Approximately 50.7% of the DDT in the contaminated field was removed 210 days after the application of Escherichia coli TG I（pDOD-gfp）. The results suggested that seeding p DOD into soil is an effective bioaugmentation method for enhancing the degradation of DDT.展开更多
基金supported by the National Natural Science Foundation of China,No.81271046the Joint Program of Beijing Municipal Natural Science Foundation(category B)Beijing Educational Committee(key project),No.KZ201510025025
文摘Several studies have investigated the protective functions of brain-derived neurotrophic factor（BDNF） in retinitis pigmentosa. However, a BDNF-based therapy for retinitis pigmentosa is not yet available. To develop an efficient treatment for fundus disease, an eukaryotic expression plasmid was generated and used to transfect human 293 T cells to assess the expression and bioactivity of BDNF on acute retinal pigment epithelial-19（ARPE-19） cells, a human retinal epithelial cell line. After 96 hours of co-culture in a Transwell chamber, ARPE-19 cells exposed to BDNF secreted by 293 T cells were more viable than ARPE-19 cells not exposed to secreted BDNF. Western blot assay showed that Bax levels were downregulated and that Bcl-2 levels were upregulated in human ARPE-19 cells exposed to BDNF. Furthermore, 293 T cells transfected with the BDNF gene steadily secreted the protein. The powerful anti-apoptotic function of this BDNF may be useful for the treatment of retinitis pigmentosa and other retinal degenerative diseases.
基金supported by the National Natural Science Foundation of China(No90713009)
文摘Green fluorescent protein（GFP） plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore（BHQ） for controlling its expression with exact spatiotemporal resolution.In vitro and in vivo experiments clearly verified that,comparing with Bhc caging, the expression level of caged GFP plasmid was dramatically decreased and then efficiently restored after subsequent photolysis.
基金supported by the National High Technology R&D Program of China (Nos. 2012AA06A204, 2013AA065202, and 2013AA102804D)the Natural Science Foundation of Zhejiang (No. LZ13D010001)
文摘A plasmid transfer-mediated bioaugmentation method for the enhancement of dichlorodiphenyltrichloroethane（DDT） degradation in soil was developed using the catabolic plasmid pDOD from Sphingobacterium sp. D-6. The p DOD plasmid could be transferred to soil bacteria, such as members of Cellulomonas, to form DDT degraders and thus accelerate DDT degradation. The transfer efficiency of pDOD was affected by the donor, temperature,moisture, and soil type. Approximately 50.7% of the DDT in the contaminated field was removed 210 days after the application of Escherichia coli TG I（pDOD-gfp）. The results suggested that seeding p DOD into soil is an effective bioaugmentation method for enhancing the degradation of DDT.
