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A ratiometric fluorescent probe for hypoxanthine detection in aquatic products based on the enzyme mimics and fluorescence of cobalt-doped carbon nitride
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作者 Xin Wang Chengyi Hong +1 位作者 Zhengzhong Lin Zhiyong Huang 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期879-884,共6页
A ratiometric fluorescent probe for hypoxanthine(Hx)detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride(Co doped g-C_(3)N_(4)).In addition... A ratiometric fluorescent probe for hypoxanthine(Hx)detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride(Co doped g-C_(3)N_(4)).In addition to emitting strong fluorescence,the peroxidase activity of Co doped g-C_(3)N_(4)can catalyze the reaction of O-phenylenediamine and H_(2)O_(2)to produce diallyl phthalate which can emit yellow fluorescence at 570 nm.Through the decomposition of Hx by xanthine oxidase,Hx can be indirectly detected by the generating hydrogen peroxide based on the measurement of fluorescent ratio I(F_(570)/F_(370)).The linear range was 1.7-272.2 mg/kg(R^(2)=0.997),and the detection limit was 1.52 mg/kg(3σ/K,n=9).The established method was applied to Hx detection in bass,grass carp,and shrimp,and the data were verified by HPLC.The result shows that the established probe is sensitive,accurate,and reliable,and can be used for Hx detection in aquatic products. 展开更多
关键词 Co doped g-C_(3)N_(4) Ratiometric fluorescent probe HYPOXANTHINE FRESHNESS Aquatic products
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Activatable fluorescent probes for imaging and diagnosis of rheumatoid arthritis
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作者 Pan Luo Fu-Qiang Gao +5 位作者 Wei Sun Jun-You Li Cheng Wang Qing-Yu Zhang Zhi-Zhuo Li Peng Xu 《Military Medical Research》 SCIE CAS CSCD 2024年第2期287-307,共21页
Rheumatoid arthritis(RA)is a systemic autoimmune disease that is primarily manifested as synovitis and polyarticular opacity and typically leads to serious joint damage and irreversible disability,thus adversely affec... Rheumatoid arthritis(RA)is a systemic autoimmune disease that is primarily manifested as synovitis and polyarticular opacity and typically leads to serious joint damage and irreversible disability,thus adversely affecting locomotion ability and life quality.Consequently,good prognosis heavily relies on the early diagnosis and effective therapeutic monitoring of RA.Activatable fluorescent probes play vital roles in the detection and imaging of biomarkers for disease diagnosis and in vivo imaging.Herein,we review the fluorescent probes developed for the detection and imaging of RA biomarkers,namely reactive oxygen/nitrogen species(hypochlorous acid,peroxynitrite,hydroxyl radical,nitroxyl),pH,and cysteine,and address the related challenges and prospects to inspire the design of novel fluorescent probes and the improvement of their performance in RA studies. 展开更多
关键词 Rheumatoid arthritis fluorescent probe IMAGING DIAGNOSIS BIOMARKER
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Temperature-controlled DCP Fluorescent Probe Based on Hydrogel-immobilized Quantum Dots Composite
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作者 童伊琳 杨坤 +2 位作者 HAN Xuecai YU Kan BAO Jiaqi 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2024年第4期931-936,共6页
A composite was created by incorporating the quantum dot-enhanced SiO_(2)nanoparticles within this hydrogel.Based on this composite,a temperature-controlled fluorescent probe for DCP was developed.A meticulous examina... A composite was created by incorporating the quantum dot-enhanced SiO_(2)nanoparticles within this hydrogel.Based on this composite,a temperature-controlled fluorescent probe for DCP was developed.A meticulous examination of this probe revealed its attributes and factors affecting its performance.By using temperature modulation,the probe was adept at detecting DCP concentrations ranging between 1.0×10^(-6)and 9.0×10^(-6)mol/L.Such a probe offers remarkable selectivity,repeatability,and robust stability,so that the detection of DCP can be carried out at different temperatures,and a fast,reliable,sensitive and low-cost intelligent detection method is realized. 展开更多
关键词 temperature-controlled fluorescent probe hydrogel-immobilized quantum dots composite DCP
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A highly sensitive fluorescent probe RN-NA reveals peroxynitrite as a novel biomarker for primary open angle glaucoma
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作者 Bo Qin Yuan Lei +3 位作者 Luo Bao You-Jia Zhang Ke-Yin Liu Yu-Hong Chen 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第2期219-227,共9页
AIM:To directly quantify peroxynitrite(ONOO-)using a highly sensitive fluorescence resonance energy transfer probe RN-NA,investigate the association between ONOOand primary open angle glaucoma(POAG),and clarify whethe... AIM:To directly quantify peroxynitrite(ONOO-)using a highly sensitive fluorescence resonance energy transfer probe RN-NA,investigate the association between ONOOand primary open angle glaucoma(POAG),and clarify whether RN-NA could be used as a potential tool for POAG diagnosis.METHODS:Plasma and aqueous humor(AH)samples were collected from POAG patients(n=100,age:59.70±6.87y)and age-related cataract(ARC)patients(n=100,age:61.15±4.60y)admitted to our hospital.Next,RN-NA was used to detect ONOO-in plasma and AH samples,and the relationship between ONOO-level and POAG was analyzed using binary logistic regression.Besides,Pearson correlation analysis was applied to characterize the correlation of the levels of ONOO-with the patients’age,intraocular pressure(IOP),and mean deviation of visual field testing.The ONOO-scavenger MnTMPyP was employed to treat the 3-morpholinosyndnomine(SIN-1)-induced ocular hypertension in mice(n=7,6-8wk).Finally,the IOP and ONOO-in both eyes were measured 30min after the last drug treatment.RESULTS:ONOO-levels of AH and plasma were significantly higher in the POAG group than in the ARC group(P<0.01).Additionally,ONOO-levels were closely correlated with POAG in a binary logistic regression analysis[odds ratio(OR)=1.008,95%confidence interval(CI):1.002-1.013,P<0.01 for AH;OR=1.004,95%CI:1.002-1.006,P<0.001 for plasma].Pearson correlation analysis showed that ONOO-levels in AH or plasma were positively associated with visual field defects(R=0.51,P<0.01 for AH;R=0.45,P<0.001 for plasma),and ONOO-levels in plasma and AH were correlated in the POAG group(R=0.69,P<0.001).However,administering MnTMPyP to mouse eyes reversed the elevated IOP caused by SIN-1(P<0.05).CONCLUSION:ONOO-levels in AH and plasma,detected by RN-NA,are significantly related to POAG and positively correlated with visual field defects in POAG patients.Hence,ONOO-is a potential biomarker of POAG,especially advanced POAG.Besides,anti-nitration compounds may be novel ocular hypotensive agents based on the animal study. 展开更多
关键词 PEROXYNITRITE fluorescent probe RN-NA primary open angle glaucoma 3-morpholinosyndnomine
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Application and development of fluorescence probes in MINFLUX nanoscopy(invited paper) 被引量:1
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作者 Jing Wang Zhen Zhang +2 位作者 Hongyu Shen Qi Wu Min Gu 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS CSCD 2023年第1期67-78,共12页
The MINimal emission FLUXes(MINFLUX)technique in optical microscopy,widely recognized as the next innovative fluorescence microscopy method,claims a spatial resolution of 1-3 nm in both dead and living cells.To make u... The MINimal emission FLUXes(MINFLUX)technique in optical microscopy,widely recognized as the next innovative fluorescence microscopy method,claims a spatial resolution of 1-3 nm in both dead and living cells.To make use of the full resolution of the MINFLUX microscope,it is important to select appropriate fluorescence probes and labeling strategies,especially in living-cell imaging.This paper mainly focuses on recent applications and developments of fluorescence probes and the relevant labeling strategy for MINFLUX microscopy.Moreover,we discuss the deficiencies that need to be addressed in the future and a plan for the possible progression of MINFLUX to help investigators who have been involved in or are just starting in the field of super-resolution imaging microscopy with theoretical support. 展开更多
关键词 fluorescence probes MINFLUX nanoscopy photoblinking super-resolution imaging labeling strategy
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Carbon Nitride Quantum Dots:A Novel Fluorescent Probe for Non-Enzymatic Hydrogen Peroxide and Mercury Detection 被引量:1
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作者 CHEN Lei LI Quan +2 位作者 WANG Xing WANG Wentai WANG Lisha 《Journal of Ocean University of China》 SCIE CAS CSCD 2023年第6期1572-1582,共11页
The mercury species in the ocean(MeHg,Hg^(2+))will be enriched in marine organisms and threaten human health through the food chain.While the excessive H_(2)O_(2)in the metabolic process will produce hydroxyl radicals... The mercury species in the ocean(MeHg,Hg^(2+))will be enriched in marine organisms and threaten human health through the food chain.While the excessive H_(2)O_(2)in the metabolic process will produce hydroxyl radicals and accelerate the aging of human cells,causing a series of diseases.Hence,the cost-effective and rapid detection of mercury and H_(2)O_(2)is of urgent requirement and significance.Here,we synthesized emerging graphitic carbon nitride quantum dots(g-CNQDs)with high fluorescence quantum yield(FLQY)of 42.69%via a bottom-up strategy by a facile one-step hydrothermal method.The g-CNQDs can detect the H_(2)O_(2)and Hg^(2+)through the fluorescence quenching effect between g-CNQDs and detected substances.With the presence of KI,g-CNQDs show concentration-dependent fluorescence toward H_(2)O_(2),with a wide detection range of 1–1000μmolL^(-1)and a low detection limit of 0.23μmolL^(-1).The g-CNQDs also show sensitivity toward Hg^(2+)with a detection range of 0–0.1μmolL^(-1)and a detection limit of 0.038μmolL^(-1).This dual-function detection of g-CNQDs has better practical application capability compared to other quantum dot detection.This study may provide a new strategy for g-CNQDs preparation and construct a fluorescence probe that can be used in various systems involving H_(2)O_(2)and Hg^(2+),providing better support for future bifunctional or multifunction studies. 展开更多
关键词 carbon nitride quantum dots hydrogen peroxide Hg2+ fluorescence probe
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A Fluorescent Probe based on a Carbazole Derivative for Detecting Brilliant Blue in Food
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作者 YANG Yunqiong YANG Jiaxin +3 位作者 FEI Shaojun ZHANG Zhirui LIU Yang ZHANG Hao 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS CSCD 2023年第2期308-311,共4页
Here a fluorescent probe based on a carbazole derivative(CNS)was developed to increase the detection range and reduce the detection limit of brilliant blue.Characteristics of CNS are studied.Due to the quenching abili... Here a fluorescent probe based on a carbazole derivative(CNS)was developed to increase the detection range and reduce the detection limit of brilliant blue.Characteristics of CNS are studied.Due to the quenching ability of colorants,CNS shows an excellent current response to brilliant blue(from 1 to 10μM)with a detection limit of 2.7×10^(-8)mol/L(3σ/k)in the conditions of a 1:1 volume ratio of water to tetrahydrofuran.And the stability and reproducibility of CNS in the detection of actual samples indicate great potential for application. 展开更多
关键词 fluorescent probe carbazole derivative brilliant blue DETECTION
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Development of taxonomic rRNA-targeted probes of two harmful algae: Prorocentrum minimum and Karenia mikimotoi by fluorescence in situ hybridization 被引量:1
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作者 CHEN Guofu WANG Quanfu +5 位作者 ZHANG Chunyun ZHANG Baoyu WANG Guangce LU Douding XU Zhong YAN Peishen 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2013年第2期66-75,共10页
Harmful algal blooms recently have been under the spotlight throughout the world, because of their nega- tive impact on the marine environment, aquaculture, fisheries as well as public health. The development of metho... Harmful algal blooms recently have been under the spotlight throughout the world, because of their nega- tive impact on the marine environment, aquaculture, fisheries as well as public health. The development of methods for rapid and precise identification and quantification of causative species is essential for the warning and monitoring of blooms, among which the techniques based on taxonomic probes are the most favored. In this study, two harmful algae, i.e., Prorocentrum minimum and Karenia mikimotoi were tak- en into consideration. The partial large subunit rDNA (D1-D2) of both species were firstly PCR-amplified, cloned and sequenced. The obtained sequences were then introduced to carry out alignment analysis for gene specific regions. Three respective candidate probes for each species were designed and used to screen the optimal probe by performing fluorescence in situ hybridization (FISH) tests. The results showed that the probes Pmin0443 and Kmik0602 displayed the best hybridization for P. minimum and K.. mikimotoi, respectively. Both the specific (taxonomic) (Pmin0443 and Kmik0602) and the control probes (UniC0512 and UniR0499) were used for cross-reactivity tests with other microalgae in our laboratory. The probes Pmin0443 and Kmik0602 are specific and could be served as taxonomic probes introduced into the tech- niques targeting rRNA, such as FISH, sandwich hybridization, and DNA-microarray assay of P minimum and K. mikimotoi in the future. Finally, FISH analyses with both probes were performed on the simulated field samples. The probes could hybridize exclusively with the target cells well, and no significant differ- ence (p 〉0.05) was observed in the cell densities of the samples determined by FISH and light microscopy (LM). All suggest that the probes are specific and could be introduced into FISH for the monitoring of both harmful algae. 展开更多
关键词 Prorocentrum minimum Karenia mikimotoi fluorescence in situ hybridization taxonomic probe
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A Fluorescence Ratiometric Probe for Cysteine/Homocysteine and Its Application for Living Cell Imaging 被引量:1
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作者 Lingliang Long Lin Wang Yanjun Wu 《International Journal of Organic Chemistry》 2013年第4期235-239,共5页
A fluorescence ratiometric probe 1 for cysteine (Cys) and homocysteine (Hcy) has been rationally constructed based on intramolecular charge transfer (ICT) mechanism. Upon treatment with Cys/Hcy, probe 1 exhibited a fl... A fluorescence ratiometric probe 1 for cysteine (Cys) and homocysteine (Hcy) has been rationally constructed based on intramolecular charge transfer (ICT) mechanism. Upon treatment with Cys/Hcy, probe 1 exhibited a fluorescence ratiometric response, with the emission wavelength displaying a large shift (from 526 nm to 446 nm). When 90 μM Cys were added, the emission ratios (I446/I526) of the probe changed dramatically from 0.01797 to 4.65472. The detection limit was also measured to be 0.18 μM (S/N = 3). The theoretical calculations have confirmed that the ratiometric response of probe 1 to Cys/Hcy is due to the inhibition of ICT process upon the reaction of probe 1 with Cys/Hcy. Furthermore, the fluorescence imaging experiments in living cell demonstrated that probe 1 was favourable for intracellular Cys/Hcy imaging. 展开更多
关键词 fluorescENT probes fluorescence CYSTEINE and HOMOCYSTEINE fluorescence IMAGING
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Fluorescence imaging of drug target proteins using chemical probes 被引量:1
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作者 Hao Zhu Itaru Hamachi 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2020年第5期426-433,共8页
Fluorescence imaging can provide valuable information on the expression,distribution,and activity of drug target proteins.Chemical probes are useful small-molecule tools for fluorescence imaging with high structural f... Fluorescence imaging can provide valuable information on the expression,distribution,and activity of drug target proteins.Chemical probes are useful small-molecule tools for fluorescence imaging with high structural flexibility and biocompatibility.In this review,we briefly introduce two classes of fluorescent probes for the visualization of drug target proteins.Enzymatically activatable probes make use of the specific enzymatic transformations that generally produce a fluorogenic response upon reacting with target enzymes.Alternatively,specific imaging can be conferred with a ligand that drives the probes to target proteins,where the labeling relies on noncovalent binding,covalent inhibition,or traceless labeling by ligand-directed chemistry. 展开更多
关键词 fluorescence imaging Drug target Chemical probe Ligand-directed chemistry
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Synthesis of 3-hydroxyflavone fluorescent probes and study of their fluorescence properties
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作者 Xiao Dong Zhao Chang Jun Sun +1 位作者 Qing Qiang Yao Wen Bao Li 《Chinese Chemical Letters》 SCIE CAS CSCD 2010年第5期529-532,共4页
Direct measurement of dipole potential in biological membranes has been impossible and 3-hydroxyflavones(3HFs) have allowed detection of changes in dipole potential in biological systems.In the present study,sixteen d... Direct measurement of dipole potential in biological membranes has been impossible and 3-hydroxyflavones(3HFs) have allowed detection of changes in dipole potential in biological systems.In the present study,sixteen derivatives of 3HF with aliphatic hydrocarbon chains of different lengths at 4′-position and 6-position were synthesized.The basic fluorescence properties of 3HFs are maintained in all the probes in terms of strong blue shift in maximum fluorescence emission wavelength and>100 fold increase in quantum yield in organic solvents and in dioleoylphosphatidylcholine(DOPC) small unilamellar vesicles(SUV) in comparison to in aqueous Hepes buffer(15 mmol/L,pH 7.4).More importantly,the ability of the new compounds to report dipole potential changes in biological systems are also maintained,since all the new probes showed spectrum properties that are similar to yet different from that of F4N1,which potentially may allow more sensitive measurement of the dipole potential change in membranes. 展开更多
关键词 3-Hydroflavones(3HFs) fluorescent probes ESIPT Dipole potential
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A KIND OF FLUORESCENCE PROBE TO STUDY THE KINETICS OF POLYMERIZATION PROCESS
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作者 杨国强 吴世康 《Chinese Journal of Polymer Science》 SCIE CAS CSCD 1994年第3期237-245,共9页
Fluorescence properties of 1-pheny1-3-(4'-nitrophenyl) pyrazoline (PNP) were studied inbulk polymerization process of methylmethacrylate (MMA). The fluorescence intensity of PNPwas enhanced and the emission maximu... Fluorescence properties of 1-pheny1-3-(4'-nitrophenyl) pyrazoline (PNP) were studied inbulk polymerization process of methylmethacrylate (MMA). The fluorescence intensity of PNPwas enhanced and the emission maximum was blue shifted with the polymerization progress. Inthe period of auto-acceleration of the polymerization the enhancement of fluorescence intensityand blue shift of peak wavelength in spectra could be observed evidently. This means that thesolvatochromic properties of PNP are influenced not only by the solvent polarity but also by theviscosity of the medium (especially by the phase transitiott). In solid state PNP emits from thecharge transfer excited state without solvent relaxation. The transient emission spectra and theresults from Bakhshiev model of solvent relaxation coincide with that from the polymerizationexperiment. 展开更多
关键词 fluorescence probe POLYMERIZATION SOLVATOCHROMISM 1-Phenyl-3- (4′-nitrophpnyl) pyrazoline
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An insight into the in vivo imaging potential of curcumin analogues as fluorescence probes
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作者 Yu Liu Chuang Zhang +3 位作者 Hao Pan Li Li Yanjie Yu Bingmi Liu 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2021年第4期419-431,共13页
Curcumin and its derivatives have good electrical and optical properties due to the highly symmetric structure of delocalized π electrons. Apart from that, curcumin and its derivatives can interact with numerous mole... Curcumin and its derivatives have good electrical and optical properties due to the highly symmetric structure of delocalized π electrons. Apart from that, curcumin and its derivatives can interact with numerous molecular targets, thereby exerting less side effects on human body. The fluorescence emission wavelength and fluorescence intensity of curcumin can be enhanced by modifying its π-conjugated system and β-diketone structure. Some curcumin-based fluorescent probes have been utilized to detect soluble/insoluble amyloid-β protein, intracranial reactive oxygen species, cysteine, cancer cells, etc. Based on the binding characteristics of curcumin-based fluorescent probes with various target molecules, the factors affecting the fluorescence intensity and emission wavelength of the probes are analyzed, in order to obtain a curcumin probe with higher sensitivity and selectivity. Such an approach will be greatly applicable to in vivo fluorescence imaging. 展开更多
关键词 CURCUMIN fluorescent probe fluorescence characteristics Structural feature
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Sign Response Mechanism of TCA Self-assembled Fluorescence Probe for Cu^2+ Detection: FRET Evidence by DFT
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作者 李福君 洒荣健 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2018年第8期1186-1200,共15页
A new type of self-assembled molecule ON-OFF fluorescence probe for toxic transition metal ions, made up of thiacalix[4]arene, micelle and fluorescence group, has been studied by DFT/TDDFT method combined with experim... A new type of self-assembled molecule ON-OFF fluorescence probe for toxic transition metal ions, made up of thiacalix[4]arene, micelle and fluorescence group, has been studied by DFT/TDDFT method combined with experiment spectra. Since the mechanism of the optical quenching signal response of such self-assembled micelle probe has always been a controversial issue of uncertainty, the spatial construction and geometric structures of the functional units of probe in the Cu2+ ion detecting process were calculated and the mechanism was investigated by the molecular transition orbital pairs method to explore the origination of ON-OFF fluorescence sign response. The results presented that the signal response mechanism of the micelle probe is ascribed to F?rster resonance energy transfer(FRET) which provides new sights different from most of the conclusions by the related research work reported. 展开更多
关键词 ON-OFF fluorescence probe density functional theory FOster resonance energytransfer heavy metal ions detection micelle self-assembled complex
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A fluorescence turn-on Hg<sup>2+</sup>probe based on rhodamine with excellent sensitivity and selectivity in living cells
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作者 Li Jiang Ling Wang +2 位作者 Bo Zhang Gui Yin Rui-Yong Wang 《Open Journal of Inorganic Chemistry》 2011年第2期16-22,共7页
A highly sensitive and selective Hg2+ probe 4 based on rhodamine-b was developed and characterized. In consideration of environmental and biological application, we connected a water soluble receptor group (sulfonated... A highly sensitive and selective Hg2+ probe 4 based on rhodamine-b was developed and characterized. In consideration of environmental and biological application, we connected a water soluble receptor group (sulfonated β-naphtol) and rhodamine-b together through hydrazine hydrate in high yield. The result turns out that this compound not only exhibits excellent sensitivity and selectivity toward Hg2+, but also shows well cell permeability and compatibility in vitro. 展开更多
关键词 fluorescence probe Mercury RHODAMINE Yeast HELA cell
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Primary amine coupling on nanocarbon catalysts: Reaction mechanism and kinetics via fluorescence probe analysis
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作者 Fan Li Xueya Dai Wei Qi 《Green Energy & Environment》 SCIE CSCD 2020年第4期453-460,共8页
Non-metallic nanocarbon materials catalyzed coupling reactions of primary amines to produce imine is an efficient,green and sustainable synthetic route,which has a wide application prospect in fine chemicals or pharma... Non-metallic nanocarbon materials catalyzed coupling reactions of primary amines to produce imine is an efficient,green and sustainable synthetic route,which has a wide application prospect in fine chemicals or pharmaceutical molecules.In the present study,we show firstly the relatively high catalytic activity of graphene oxide in the reaction of oxidative coupling of benzylamine(OCB),which is even comparable with typical metal-based catalysts,indicating the great potential of nanocarbon materials in this reaction system.More importantly,a novel twophoton fluorescence probe molecule(N-propyl-4-hydrazinyl-1,8-naphthalimide,NA)with special chemical structure of hydrazine functionality was synthesized.The probe NA could selectively react with aldehyde or ketone compounds,leading to the photoluminescence enhancement via inhibition of photo induced electron transfer(PET)process.The synthesized NA was applied as probe in carbon catalyzed OCB system to predict the existence of reaction intermediate benzaldehyde(BA),indicating the reaction pathway of oxidation-deamination-condensation in nanocarbon catalyzed OCB process.The proposed luminescence-probe strategy for revealing the kinetics and mechanism may also shed light in other reaction systems concerning the intermediates or products of ketones or aldehydes. 展开更多
关键词 NANOCARBON Benzylamine coupling reaction fluorescence probe Reaction mechanism
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A Novel Fluorescence Probe 9-(4-(1,2-diamine)benzeneN^1-phenyl)acridine for Nitric Oxide Determination
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作者 丁莉芸 袁芳 +3 位作者 HUANG Lanfen HUANG Jun LIU Xiaofang 梁兵 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2014年第4期848-853,共6页
A novel fluorescent probe 9-(4-(1,2-diamine)benzene-N1-phenyl)acridine(DABPA) was synthesized for the detection of nitric oxide(NO) and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelect... A novel fluorescent probe 9-(4-(1,2-diamine)benzene-N1-phenyl)acridine(DABPA) was synthesized for the detection of nitric oxide(NO) and characterized by IR, 1H-NMR and EI-MS spectroscopy. Based on a photoelectron transfer mechanism, the fl uorescence intensities of DABPA were investigated with the different concentrations of NO. Under the optimal experimental conditions, the fl uorescence intensity of DABPA had a good linear relationship(R2=0.9977) with NO concentration in the range from 1×10-7 to 1.5×10-6 mol/L with a detection limit of 1×10-8 mol/L. The cytotoxicity induced by DABPA was evaluated by the MTT(3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide) assay for biological application. Furthermore, the probe DABPA had also been successfully applied to real-time image NO produced in PC12 cells in the presence of L-arginine. 展开更多
关键词 nitric oxide fluorescent probe 9-(4-(1 2-diamine)benzene-N1-phenyl)acridine
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A Fluorescence Ratiometric Probe for Detection of Cyanide in Water Sample and Living Cells
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作者 Lingliang Long Lin Wang Yanjun Wu 《Advances in Materials Physics and Chemistry》 2013年第8期307-313,共7页
In the present work, Compound 1 has been synthesized as a novel fluorescence ratiometric probe for CN-. Upon treatment with CN-, Probe 1 exhibited a fluorescence ratiometric response, with the emission wavelength shif... In the present work, Compound 1 has been synthesized as a novel fluorescence ratiometric probe for CN-. Upon treatment with CN-, Probe 1 exhibited a fluorescence ratiometric response, with the emission wavelength shift from 570 nm to 608 nm. When 90 μM CN-?was introduced, the emission ratios (I570/I608) of the probe changed dramatically from 0.52156 to 4.21472. The detection limit was also measured to be 0.24 μM (S/N = 3). In addition, Probe 1 had a selective response to CN-, while other anions caused nearly no interference. The sensing reaction product of Probe 1 with CN-?was characterized by 1H NMR spectra and ESI Mass spectrometry. Furthermore, Probe 1 has been successfully applied to detect CN-?in natural water samples. The fluorescence imaging experiments in living cells also demonstrated that Probe 1 could monitor CN-?in biological samples. 展开更多
关键词 ORGANIC fluorescence Materials fluorescENT probes CYANIDE fluorescence Imaging
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SPECTROSCOPIC DETERMINATION OF THE AVERAGE NUMBER OF COAGGREGATES (Nco) OF CHOLESTERYL ESTERS AND THE FLUORESCENCE PROBE
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作者 Xi Kui JIANG Jin Tao ZHANG Shanghai Institute of Organic Chemistry,Academia Sinica,345 Ling-ling LU,Shanghai 200032 Zhen He TONG The Laboratory of Photochemistry,Institute of Photographio Chemistry,Academia Sinica,Beijing 100101 《Chinese Chemical Letters》 SCIE CAS CSCD 1992年第9期673-674,共2页
Average aggregate number of coaggregates(N_co)of CE-n or BL-n and the fluoresc- ence probe(Np-16)have been determined by using time-resolved fluorescence spectroscopy. Chain-length,hydroxy-group and chain-foldability ... Average aggregate number of coaggregates(N_co)of CE-n or BL-n and the fluoresc- ence probe(Np-16)have been determined by using time-resolved fluorescence spectroscopy. Chain-length,hydroxy-group and chain-foldability effects on the N_co have been discussed. 展开更多
关键词 CE NCO OF CHOLESTERYL ESTERS AND THE fluorescence probe SPECTROSCOPIC DETERMINATION OF THE AVERAGE NUMBER OF COAGGREGATES
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Lighting up plants with near-infrared fluorescence probes
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作者 Yida Pang Mengjiao Lu +4 位作者 Hyeonji Rha Wenchao Yang Amit Sharma Yao Sun Jong Seung Kim 《Science China Chemistry》 SCIE EI CAS CSCD 2024年第3期774-787,共14页
Fluorescence imaging is a non-invasive and highly sensitive bioimaging technique that has shown remarkable strides in plant science. It enables real-time monitoring and analysis of biological and pathological processe... Fluorescence imaging is a non-invasive and highly sensitive bioimaging technique that has shown remarkable strides in plant science. It enables real-time monitoring and analysis of biological and pathological processes in plants by labeling specific molecular or cellular structures with fluorescent probes. However, tissue scattering and phytochrome interference have been obstacles for conventional fluorescence imaging of plants in the ultraviolet and visible spectrum, resulting in unsatisfactory imaging quality. Fortunately, advances in near-infrared(NIR) fluorescence imaging technology(650-900 nm) offer superior spatial-temporal resolution and reduced tissue scattering, which is sure to improve plant imaging quality. In this review, we summarize recent progress in the development of NIR fluorescence imaging probes and their applications for in vivo plant imaging and the identification of plant-related biomolecules. We hope this review provides a new perspective for plant science research and highlights NIR fluorescence imaging as a powerful tool for analyzing plant physiology, adaptive mechanisms, and coping with environmental stress in the near future. 展开更多
关键词 NIR fluorescent probe PLANT imaging biologic application
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