Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential.Structural and functional characterization of drought stress-induced genes has contributed to a bette...Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential.Structural and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants respond and adapt to the drought stress.In the present study,differential display technique was employed to study the gene expression of rice plants at the reproductive stage that were subjected to drought stress by withholding water,Pseudomonas fluorescens strain(Pf1) treated plants subjected for drought stress by withholding water and control(well-watered).Differentially expressed c DNAs of six genes(COX1,PKDP,b ZIP1,AP2-EREBP,Hsp20 and COC1) were identified,cloned and sequenced.Real-time q PCR analysis showed that all the six genes were upregulated in drought-stressed plants treated with Pf1.This revealed that the remarkable influence of Pf1 colonization leads to drought tolerance at the reproductive stage.These results showed that high levels of gene expression in plants lacking adequate water can be remarkably influenced by Pf1 colonization,which might be a key element for induced systemic tolerance by microbes.展开更多
在樱桃病毒A(CVA)mp基因保守区域设计了3对检测引物,经特异性筛选后,获得可用于病毒定量研究的引物。制备质粒标准品,建立标准曲线,同时验证该方法的灵敏度和特异性,并应用于田间果树样品CVA定量检测。最终成功筛选出1对检测效率高、特...在樱桃病毒A(CVA)mp基因保守区域设计了3对检测引物,经特异性筛选后,获得可用于病毒定量研究的引物。制备质粒标准品,建立标准曲线,同时验证该方法的灵敏度和特异性,并应用于田间果树样品CVA定量检测。最终成功筛选出1对检测效率高、特异性强的引物(CVA-dF2、CVA-dR2),基于SYBR Green I荧光染料建立反转录实时荧光定量PCR检测CVA的方法。该方法重复性好、灵敏度高,无需借助内参基因即可准确检测目的病毒载量,绝对定量标准曲线斜率为-3.5746,决定系数R2为0.9986,扩增效率为0.9044,比常规RT-PCR检测灵敏度高10倍。该方法的建立为CVA定量研究提供了有力工具,可用于果树中CVA批量检测或低丰度病毒样品检测。展开更多
基金the Jawaharlal Nehru University(JNU) research fellowship sponsored by the Department of Biotechnology(DBT),Government of India
文摘Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential.Structural and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants respond and adapt to the drought stress.In the present study,differential display technique was employed to study the gene expression of rice plants at the reproductive stage that were subjected to drought stress by withholding water,Pseudomonas fluorescens strain(Pf1) treated plants subjected for drought stress by withholding water and control(well-watered).Differentially expressed c DNAs of six genes(COX1,PKDP,b ZIP1,AP2-EREBP,Hsp20 and COC1) were identified,cloned and sequenced.Real-time q PCR analysis showed that all the six genes were upregulated in drought-stressed plants treated with Pf1.This revealed that the remarkable influence of Pf1 colonization leads to drought tolerance at the reproductive stage.These results showed that high levels of gene expression in plants lacking adequate water can be remarkably influenced by Pf1 colonization,which might be a key element for induced systemic tolerance by microbes.
文摘在樱桃病毒A(CVA)mp基因保守区域设计了3对检测引物,经特异性筛选后,获得可用于病毒定量研究的引物。制备质粒标准品,建立标准曲线,同时验证该方法的灵敏度和特异性,并应用于田间果树样品CVA定量检测。最终成功筛选出1对检测效率高、特异性强的引物(CVA-dF2、CVA-dR2),基于SYBR Green I荧光染料建立反转录实时荧光定量PCR检测CVA的方法。该方法重复性好、灵敏度高,无需借助内参基因即可准确检测目的病毒载量,绝对定量标准曲线斜率为-3.5746,决定系数R2为0.9986,扩增效率为0.9044,比常规RT-PCR检测灵敏度高10倍。该方法的建立为CVA定量研究提供了有力工具,可用于果树中CVA批量检测或低丰度病毒样品检测。
文摘通过模拟低压低氧(7000±50 m)考察谷胱苷肽转移酶p1基因(G lu tath ione S-transferase p1,G STP 1)对低压低氧的敏感性,以探讨G STP 1在机体适应急性低压低氧环境中的分子机制。30只雄性SD大鼠随机分为5组:0、1、3、5、7 d组,每天在模拟7000±50 m高度的低压低氧舱内暴露12 h,连续暴露6 h后,休息1 h,再继续暴露6h。采用荧光定量-反转录-聚合酶链反应(FQ-RT-PCR)技术对急性低压低氧大鼠模型肺组织中G STP 1基因表达进行定量分析,同时用分光光度法测定了肺组织中谷胱苷肽转移酶(g lu tath ione S-transferases,G ST s)的活性和丙二醛(M a le ic d ia ldehyde,M DA)的变化。结果显示,与未暴露组相比,G STP 1基因从第1 d到第7 d的表达有显著性差异(P<0.05);G ST s活性从第1 d到第7 d呈下降趋势,M DA呈上升趋势,与未暴露组相比,均有显著性差异(P<0.05)。结果表明:G STP 1基因对低压低氧敏感,并可考虑作为机体适应特殊环境进行基因选材的一个新指标。
基金Supported by the Food Safety Key Technology Project in the National Science and Technology Pillar Program during the 11th Five-Year Plan Period(2006 BAK02A03)Anhui University Provincial Natural Science Research Project(KJ2007B294)
