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厌氧颗粒污泥中微生物种群变化的分子生物学解析 被引量:29
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作者 孙寓姣 左剑恶 +1 位作者 李建平 鲁颐琼 《中国环境科学》 EI CAS CSCD 北大核心 2006年第2期183-187,共5页
荧光定量-聚合酶链式反应(RTQ-PCR)与荧光原位杂交(FISH)技术对厌氧反应器内不同状态下颗粒污泥中微生物种群的数量变化与空间分布进行研究.结果发现,颗粒污泥中真细菌明显多于古细菌,但随着厌氧反应器有机负荷的提高,古细菌明显增加,... 荧光定量-聚合酶链式反应(RTQ-PCR)与荧光原位杂交(FISH)技术对厌氧反应器内不同状态下颗粒污泥中微生物种群的数量变化与空间分布进行研究.结果发现,颗粒污泥中真细菌明显多于古细菌,但随着厌氧反应器有机负荷的提高,古细菌明显增加,其中产甲烷丝菌也明显增加;真细菌多分布生长在颗粒污泥的外层,而对环境条件敏感的古细菌多分布生长在内层,且随着反应器有机负荷的提高,这种层状分布的特点更为明显. 展开更多
关键词 厌氧反应器 颗粒污泥 实时定量-聚合酶链式反应(RTQ-PCR) 荧光原位杂交(fish) 微生物种群变化
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13q14断裂重排与非小细胞肺癌转移潜能关系的研究 被引量:1
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作者 黄昀 杨焕杰 +2 位作者 金焰 李慧敏 傅松滨 《遗传》 CAS CSCD 北大核心 2005年第4期531-534,共4页
发生转移的肿瘤细胞经常存在染色体数目异常和结构畸变,在多种有转移潜能的肿瘤细胞中都涉及到13q14的异常。以往研究表明,在同一组织来源但转移潜能不同的肺腺癌细胞系AGZY83a和Anip973中存在13q14的断裂重排。采用mRNA差异展示技术(mR... 发生转移的肿瘤细胞经常存在染色体数目异常和结构畸变,在多种有转移潜能的肿瘤细胞中都涉及到13q14的异常。以往研究表明,在同一组织来源但转移潜能不同的肺腺癌细胞系AGZY83a和Anip973中存在13q14的断裂重排。采用mRNA差异展示技术(mRNADD)分析这一对细胞系得到的差异表达基因BRI基因位于13q14。为了进一步分析肺癌细胞的转移潜能与13q14断裂重排间的关系,采用13q涂染探针对具有不同转移潜能的非小细胞肺癌细胞系PAa、SPC1A和95D中期分裂相进行G显带后的荧光原位杂交分析。结果发现,在3个肺癌细胞系中有多种13号染色体长臂的结构异常,其中此3个细胞系均涉及13q3233的频发断裂。但是低转移肺癌细胞系PAa、SPC1A均未涉及13q14的断裂,而高转移肺癌细胞系95D的两种细胞克隆均可见13q14的断裂。提示13q14断裂点与肺癌细胞的转移能力有一定的相关性,两者之间的遗传学意义需要进一步研究探索。 展开更多
关键词 非小细胞肺癌 染色体重排 转移 染色体涂染 荧光原位杂交
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Application of non-small cell lung cancer pleural effusion cell blocks in molecular pathological detection 被引量:1
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作者 Ying Zhang Nan Jiang +4 位作者 Dongdong Qian Xiangzhou Li Yu Zhou Jia Mei Xiaohui Cao 《The Chinese-German Journal of Clinical Oncology》 CAS 2014年第4期157-161,共5页
Objective: The tumor tissues used in molecular pathological detection were usually obtained by surgery, which would cause trauma and may not be suitable for the terminal cancer patients. This paper evaluated the value... Objective: The tumor tissues used in molecular pathological detection were usually obtained by surgery, which would cause trauma and may not be suitable for the terminal cancer patients. This paper evaluated the value of the non-small cell lung cancer(NSCLC) pleural effusion cell blocks as tumor tissues replacement materials in the application of molecular pathological detection. Methods: Tumor cells were made into cell blocks through stratified centrifugal from 30 NSCLC patients with the pleural effusion. The immunohistochemistry, fluorescence in situ hybridization(FISH) and gene sequencing methods were employed in our experiments. Results: The tumor cells of cell block section were rich and could keep part of histological structure. Immunohistochemistry staining could assist diagnosis and tumor parting. Epidermal growth factor receptor(EGFR) FISH-positive was found in 33.33% of the group, high polysomy in 6 cases, amplification in 4 cases. EGFR gene mutations were found in 8 cases of 30 samples, with an incidence of 26.67%, 6 cases were detected in the exon 19, and 2 cases were detected in the exon 21. Conclusion: The NSCLC pleural effusion cell blocks are useful for the diagnosis and determining the primary source of tumor, instructed targeted therapy. 展开更多
关键词 CARCINOMA non-small cell lung (NSCLC) pleural effusion cell block IMMUNOHISTOCHEMISTRY fluorescence insitu hybridization (fish MUTATION
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Molecular Cytogenetic Characterization and Stem Rust Resistance of Five Wheat-Thinopyrum ponticum Partial Amphiploids 被引量:9
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作者 Qi Zheng Zhenling Lv +5 位作者 Zhixia Niu Bin Li Hongwei Li Steven S.Xu Fangpu Han Zhensheng Li 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2014年第11期591-599,共9页
Partial amphiploids created by crossing common wheat (Triticum aestivum L.) and Thinopyrum ponticum (Podp.) Barkworth & D. R. Dewey are important intermediates in wheat breeding because of their resistance to maj... Partial amphiploids created by crossing common wheat (Triticum aestivum L.) and Thinopyrum ponticum (Podp.) Barkworth & D. R. Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases. In this study, we examined the chromosome compositions of five Xiaoyan-series wheat-Th, ponticum partial amphiploids (Xiaoyan 68, Xiaoyan 693, Xiaoyan 784, Xiaoyan 7430, and Xiaoyan 7631) using GISH, multicolor-GISH, and multicolor-FISH. We found several chromosome changes in these lines. For example, wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430, respectively, while wheat chromosome 6B was eliminated from Xiaoyan 693 and Xiaoyan 7631. Chromosome rearrangements were also detected in these amphiploids, including an interspecific translocation involving chromosome 4D and some intergenomic translocations, such as A--B and A--D translocations, among wheat genomes. Analysis of the Th. ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes. We also evaluated these partial amphiploids for resistance to nine races of stem rust, including TTKSK (commonly known as Ug99). Three lines, Xiaoyan 68, Xiaoyan 784, and Xiaoyan 7430, exhibited excellent resistance to all nine races, and could therefore be valuable sources of stem rust resistance in wheat breeding. 展开更多
关键词 Thinopyrum ponticum Triticum aestivum Partial amphiploid Multicolor-genomic in situ hybridization (mc-GISH) Multicolor-fluorescent insitu hybridization (me-fish) Stem rust
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