Fluphenazine (FP) treatment (50mg/kg bw, ip in saline) 30 min before or 6 or 10 h after CCl4 administration (1 ml/kg ip in olive oil) significantly prevented the liver necrosis produced by the hepatotoxin at 24 h. FP ...Fluphenazine (FP) treatment (50mg/kg bw, ip in saline) 30 min before or 6 or 10 h after CCl4 administration (1 ml/kg ip in olive oil) significantly prevented the liver necrosis produced by the hepatotoxin at 24 h. FP had enhancing effects on the covalent binding of CCl4 reactive metabolites to cellular constituents and on CCl4 induced lipid peroaldation.FP lowered bOdy temperature of the CCl4-poisoned animals during the 24 h observation period. The obtained results are compatible but do not prove the hypothesis that calmodulin (CaM) had participation in late occurring events preceding necrosis. FP lowering action on body temperature, however, might also play a role in the effects of this drug on the onset of CCl4 induced liver necrosis. FP levels in liver tissue as determined by gas chromatography-mass spectrometry evidenced the presence of the drug in amounts suffi cient to inhibit CaM and that suggests that not all preventive effects of FP are due to its indirect actions on the central nervous system via decreased body temperature展开更多
A new procedure based on generation and subsequent reduction of orange-colored fluphenazine hydrochloride radical （FPH^＋） is presented for the screening of total antioxidant capacity （TAC） of various fruit matric...A new procedure based on generation and subsequent reduction of orange-colored fluphenazine hydrochloride radical （FPH^＋） is presented for the screening of total antioxidant capacity （TAC） of various fruit matrices. The FPH^＋ was obtained by mixing fluphenazine hydrochloride with persulfate （final concentration 2 mmol/L and 0.05 mmol/L, respectively） in 3 mol/L H2SO4 with constant shaking for 5 min. The solution formed showed maximum absorption as 0.8 ＋ 0.02 at 500 nm in first-order derivative spectrum. The percent inhibition of the solution increased linearly on addition of increasing amounts of standard antioxidants i.e., ascorbic acid etc. The TACs of sample citrus juices were calculated in terms of ascorbic acid equivalents （AAEs） by comparing their inhibition curves with that of ascorbic acid. Comparison of AAE values of different commercial orange juices using the newly developed FPH^＋ assay and the well-known ABTS/K2S2Os and DMPD/FeC13 assays indicated the precision and comparable sensitivity of the method. The proposed procedure is quick, economical, and more precise and gives results comparable to contemporary assays.展开更多
文摘Fluphenazine (FP) treatment (50mg/kg bw, ip in saline) 30 min before or 6 or 10 h after CCl4 administration (1 ml/kg ip in olive oil) significantly prevented the liver necrosis produced by the hepatotoxin at 24 h. FP had enhancing effects on the covalent binding of CCl4 reactive metabolites to cellular constituents and on CCl4 induced lipid peroaldation.FP lowered bOdy temperature of the CCl4-poisoned animals during the 24 h observation period. The obtained results are compatible but do not prove the hypothesis that calmodulin (CaM) had participation in late occurring events preceding necrosis. FP lowering action on body temperature, however, might also play a role in the effects of this drug on the onset of CCl4 induced liver necrosis. FP levels in liver tissue as determined by gas chromatography-mass spectrometry evidenced the presence of the drug in amounts suffi cient to inhibit CaM and that suggests that not all preventive effects of FP are due to its indirect actions on the central nervous system via decreased body temperature
文摘A new procedure based on generation and subsequent reduction of orange-colored fluphenazine hydrochloride radical （FPH^＋） is presented for the screening of total antioxidant capacity （TAC） of various fruit matrices. The FPH^＋ was obtained by mixing fluphenazine hydrochloride with persulfate （final concentration 2 mmol/L and 0.05 mmol/L, respectively） in 3 mol/L H2SO4 with constant shaking for 5 min. The solution formed showed maximum absorption as 0.8 ＋ 0.02 at 500 nm in first-order derivative spectrum. The percent inhibition of the solution increased linearly on addition of increasing amounts of standard antioxidants i.e., ascorbic acid etc. The TACs of sample citrus juices were calculated in terms of ascorbic acid equivalents （AAEs） by comparing their inhibition curves with that of ascorbic acid. Comparison of AAE values of different commercial orange juices using the newly developed FPH^＋ assay and the well-known ABTS/K2S2Os and DMPD/FeC13 assays indicated the precision and comparable sensitivity of the method. The proposed procedure is quick, economical, and more precise and gives results comparable to contemporary assays.
