To study the mechanism of repair of pancreas following acute pancreatitis (AP).Methods NIH Swiss mice were randomized into the saline control group and AP group.AP was induced by intraperitoneal injection of 50 ug/kg ...To study the mechanism of repair of pancreas following acute pancreatitis (AP).Methods NIH Swiss mice were randomized into the saline control group and AP group.AP was induced by intraperitoneal injection of 50 ug/kg body weight cearulein for 4 times,once per hour.The cellular proliferation was detected using flow cytometry.Collagen Ⅰ was detected with immunohistochemistry.Protein zymography was used to evaluate the expression of matrix metalloproteinase-3 (MMP-3).Results In the AP group,the pancreatic lesions were the severest in 8 h and then recovered on the 7th d after induction of AP.Flow cytometry showed that there were three stages of cell proliferation after AP:the active proliferation in early stage,inhibition of proliferation in middle stage and high proliferation in the late stage.The expression level of collage Ⅰ and MMP-3 was markedly higher in the AP group than in the saline control group.Conclusion The pancreas can be repaired after AP.The regeneration mechanism includes intersitial fibrosis and acinar cell replication.7 refs,4 figs.展开更多
文摘To study the mechanism of repair of pancreas following acute pancreatitis (AP).Methods NIH Swiss mice were randomized into the saline control group and AP group.AP was induced by intraperitoneal injection of 50 ug/kg body weight cearulein for 4 times,once per hour.The cellular proliferation was detected using flow cytometry.Collagen Ⅰ was detected with immunohistochemistry.Protein zymography was used to evaluate the expression of matrix metalloproteinase-3 (MMP-3).Results In the AP group,the pancreatic lesions were the severest in 8 h and then recovered on the 7th d after induction of AP.Flow cytometry showed that there were three stages of cell proliferation after AP:the active proliferation in early stage,inhibition of proliferation in middle stage and high proliferation in the late stage.The expression level of collage Ⅰ and MMP-3 was markedly higher in the AP group than in the saline control group.Conclusion The pancreas can be repaired after AP.The regeneration mechanism includes intersitial fibrosis and acinar cell replication.7 refs,4 figs.
