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双酚A残留酶联免疫分析方法的研究及应用 被引量:7
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作者 张丽莎 吕型超 +3 位作者 陈涵倩 邓杰 潘熙萍 施曼玲 《核农学报》 CAS CSCD 北大核心 2014年第2期234-239,共6页
为了建立双酚A残留的酶联免疫分析方法,采用碳化二亚胺法将半抗原双酚酸(BHPVA)分别与卵清蛋白(OVA)和牛血清蛋白(BSA)偶联,制备免疫原BHPVA-OVA和包被原BHPVA-BSA。基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)检测表明BHPVA与BSA... 为了建立双酚A残留的酶联免疫分析方法,采用碳化二亚胺法将半抗原双酚酸(BHPVA)分别与卵清蛋白(OVA)和牛血清蛋白(BSA)偶联,制备免疫原BHPVA-OVA和包被原BHPVA-BSA。基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)检测表明BHPVA与BSA和OVA的偶联比分别为12∶1和16∶1。BHPVA-OVA免疫新西兰大白兔获得抗双酚A的多克隆抗体,抗体ELISA效价为1∶1×107。基于该抗体建立双酚A间接竞争ELISA(ciELISA)检测方法,其检测的线性范围为0.02-100ng·mL-1,线性回归方程y=9.0326 ln x+39.105,R2=0.9975,双酚A的检测限为IC10=0.04ng·mL-1。采用所建立的ciELISA方法均检测出8种塑料食品袋中的双酚A残留,迁移析出量分别从4.9到31mg·L-1,批内和批间变异系数均小于12.48%,说明本试验所建立ciELISA法可有效地用于塑料食品袋中的双酚A迁移量的测定,不仅具有高度的灵敏性,而且具有较好的稳定性。 展开更多
关键词 双酚A 多克隆抗体 间接竞争酶联免疫吸附法 塑料食品袋
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固相微萃取-气相色谱-质谱法测定食品塑料包装袋中6种酞酸酯类化合物 被引量:7
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作者 陈德文 廖新艳 +2 位作者 王正林 周江 王国建 《食品科学》 EI CAS CSCD 北大核心 2014年第6期125-128,共4页
建立固相微萃取-气相色谱-质谱同时测定食品塑料包装袋中6种酞酸酯类化合物的分析方法,并对固相微萃取方式、萃取溶剂、萃取头涂层、萃取温度等参数进行优化。采用85μm PA固相微萃取纤维头、正己烷为萃取溶剂,90℃平衡5min后萃取吸附... 建立固相微萃取-气相色谱-质谱同时测定食品塑料包装袋中6种酞酸酯类化合物的分析方法,并对固相微萃取方式、萃取溶剂、萃取头涂层、萃取温度等参数进行优化。采用85μm PA固相微萃取纤维头、正己烷为萃取溶剂,90℃平衡5min后萃取吸附30min,在250℃进样口解吸5min后供气相色谱-质谱分析。结果表明:该方法的线性范围为0.25-50mg/L,方法检出限为0.049-0.920mg/L,回收率为82.2%-109.0%,相对标准偏差(n=6)为4.0%-12.0%,该方法能很好地富集基体中的目标化合物,满足食品塑料包装袋中多种酞酸酯类化合物的分析要求。 展开更多
关键词 固相微萃取 气相色谱-质谱法 食品塑料包装袋 酞酸酯类化合物
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Consumer willingness to pay for plastic bags levy and willingness to accept eco-friendly alternatives in Botswana 被引量:1
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作者 Patricia K.Madigele Goemeone E.J Mogomotsi Mavis Kolobe 《Chinese Journal of Population,Resources and Environment》 2017年第3期255-261,共7页
Plastic bags, though commonly used for packaging of goods, are highly durable and non- biodegradable. Plastic bag litter serves as a negative environmental externality detrimental to animal health and natural beauty o... Plastic bags, though commonly used for packaging of goods, are highly durable and non- biodegradable. Plastic bag litter serves as a negative environmental externality detrimental to animal health and natural beauty of the environment. In an effort to curtail the excessive use of plastic bags in Botswana, the government introduced a plastic levy targeted at consumers at retail points. This study uses primary data collected from 367 respondents randomly selected from six geospatially and purposively selected shopping malls to determine the consumers' willingness to pay (WTP) for continued plastic bag usage and their willingness to accept (WTA) to shift to eco-friendly alternatives. The results of the study revealed that open dumping and burning of plastic litter are more common in Maun than in Gaborone, It also showed that there is a significant relationship between the level of education and W'FP. Further, there is a significant variation in income and education in respect to environmental awareness. The government should improve its efforts of sensitizing the public about the improper disposal of plastic bags. 展开更多
关键词 Willingness to pay willingness to accept plasticbags plastic bag levy environmental externality
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