爱德华·摩根·福斯特(Edward Morgan Forster,1879—1970)是20世纪英国最杰出的小说家、散文家之一。国外关于福斯特的研究最早兴起于英美国家,大约出现于20世纪30、40年代,早期的评论主要有F·R·利维斯(F.R.Leavis)...爱德华·摩根·福斯特(Edward Morgan Forster,1879—1970)是20世纪英国最杰出的小说家、散文家之一。国外关于福斯特的研究最早兴起于英美国家,大约出现于20世纪30、40年代,早期的评论主要有F·R·利维斯(F.R.Leavis)和莱昂内尔·特里林(Lionel Trilling)的评论文章和著作。展开更多
Semiconductor QDs have emerged as a novel class of fluorophore with unique photoluminescence properties, in particular, CdSe/ZnS core-shell QDs have been successfully used as biocompatible fluorescence resonance energ...Semiconductor QDs have emerged as a novel class of fluorophore with unique photoluminescence properties, in particular, CdSe/ZnS core-shell QDs have been successfully used as biocompatible fluorescence resonance energy transfer donors. Here we report FRET between CdSe/ZnS core-shell QDs (donor) and organic dye fluorescein 27 (F27) (acceptor). The results demonstrate the occurrence of efficient energy transfer in the system and the FRET efficiency is not only influenced by the spectral overlap between the QD donor emission and acceptor absorption, it might depend on QDs surface effect also. Efforts are made to correlate quantitatively spectral dependence of FRET rate with acceptor absorption spectrum, Forster distance, transfer efficiency (E) obtained employing steady-state & time-resolved technique.展开更多
Enzymes are the major players for many biological processes.Fundamental studies of the enzymatic activity at the single-molecule level provides important information that is otherwise inaccessible at the ensemble leve...Enzymes are the major players for many biological processes.Fundamental studies of the enzymatic activity at the single-molecule level provides important information that is otherwise inaccessible at the ensemble level.Yet,these single-molecule experiments are technically di±cult and generally require complicated experimental design.Here,we develop a Holliday junction(HJ)-based platform to study the activity of restriction endonucleases at the single-molecule level using single-molecule FRET(sm-FRET).We show that the intrinsic dynamics of HJ can be used as the reporter for both the enzyme-binding and the substrate-release events.Thanks to the multiple-arms structure of HJ,the fluorophore-labeled arms can be different from the surface anchoring arm and the substrate arm.Therefore,it is possible to independently change the substrate arm to study different enzymes with similar functions.Such a design is extremely useful for the systematic study of enzymes from the same family or enzymes bearing different pathologic mutations.Moreover,this method can be easily extended to study other types of DNA-binding enzymes without too much modi fication of the design.We anticipate it can find broad applications in single-molecule enzymology.展开更多
In the course of pushing forward the overall growth capacities of the mining industry and getting adapted to the tense competition after China抯 entry into WTO, it is quite necessary to speed up the process of fosteri...In the course of pushing forward the overall growth capacities of the mining industry and getting adapted to the tense competition after China抯 entry into WTO, it is quite necessary to speed up the process of fostering large state-owned coal group enter-prises. This strategical proposal is made on the basis of a comprehensive analysis of the main problems challenges faced by the coal mining industry is made on. The main prob-lems with Chinese coal mining industries discussed include: over-competition with a low convergence, severe waste and loss of mine resources with poor exploitation techniques, disordered operation with few industrial entry barriers, heavy welfare burdens from low socialization. The accession of WTO provide Chinese coal mining industry with chances to push forward the reforms and structural shift in the enterprises, expedite the estab-lishment and perfect of coal mining market mechanism, expand their international market share as well as introduce foreign investment, and with challenges from high technology, knowledge economy, and information revolution. The solutions to the foster of large-scale coal mining group enterprises, as well as the possible points to note and the policy pro-posal are advanced.展开更多
W942715 小麦族耐胁迫性的遗传工程[刊,英]/Forster,B.P.//Proceedings of the Royal Society ofEdinburgh.Section B,Biological Sciences.-1992,99(3/4).-89～106[WBTA,1993,10(3).2550]W942716 入选捷克斯洛伐克春大麦品种对白粉病...W942715 小麦族耐胁迫性的遗传工程[刊,英]/Forster,B.P.//Proceedings of the Royal Society ofEdinburgh.Section B,Biological Sciences.-1992,99(3/4).-89～106[WBTA,1993,10(3).2550]W942716 入选捷克斯洛伐克春大麦品种对白粉病的抗性[刊,捷]/Dreiseitl Antonin…//Genet.aslecht..-1993.29(2).-123～130 [493,1993,(11/12),176]W942717 捷克斯洛伐克春大麦品种对白粉病抗性的评价[刊,捷]/Dreiseitl,A.//Rostl.vyroba.-1993,39(4).-337～344[484,1993,(11/12).153]展开更多
《阜阳职业技术学院学报》2023年第2期第66-70页刊发刘知国撰写的“生态女性主义视角下的爱德华·福斯特小说研究”一文,英文目次页中题名译文为“A Study of Edward Forster's Novels from the Perspective of Ecofeminism”,...《阜阳职业技术学院学报》2023年第2期第66-70页刊发刘知国撰写的“生态女性主义视角下的爱德华·福斯特小说研究”一文,英文目次页中题名译文为“A Study of Edward Forster's Novels from the Perspective of Ecofeminism”,现更改为“Exploration on E·M·Forster’s Novels in Perspective of Ecofeminism”。特此声明。展开更多
This paper analyses the female characters in E.M.Forster’s novels from the perspective of feminism,through the deep exploration of E.M.Forster’s works,it is evident to draw a conclusion that there are four kinds of ...This paper analyses the female characters in E.M.Forster’s novels from the perspective of feminism,through the deep exploration of E.M.Forster’s works,it is evident to draw a conclusion that there are four kinds of female characters:first of all,stubborn and arrogant old ladies;the second type is insensitive but self-awakening middle-aged ladies;the third type is knowledgeable and smart young ladies;the last one is the warm-hearted and benevolent lady.These four kinds of different female characters can reveal that E.M.Forster has a contradictory attitude towards women.For one thing,he respected and sympathized with women,for another he discriminated against women.展开更多
In Forster's masterpiece A Passage to India, the novelist deals in large parts with the contemporary Indian society under British rule and the complicated relations between the English and the native population. I...In Forster's masterpiece A Passage to India, the novelist deals in large parts with the contemporary Indian society under British rule and the complicated relations between the English and the native population. In this novel, Forster explores the difficulty to unify human relations,and exposes the eternal gaps between two nations. This paper will center on the eternal gaps of a Passage to India, which are represented by the misunderstandings between"India"and"British". By exploring and analyzing those gaps, the paper reaches the conclusion that those gaps can not be eliminated and the long-existing communication proposed by Forster is still a long way to go.展开更多
文摘爱德华·摩根·福斯特(Edward Morgan Forster,1879—1970)是20世纪英国最杰出的小说家、散文家之一。国外关于福斯特的研究最早兴起于英美国家,大约出现于20世纪30、40年代,早期的评论主要有F·R·利维斯(F.R.Leavis)和莱昂内尔·特里林(Lionel Trilling)的评论文章和著作。
文摘Semiconductor QDs have emerged as a novel class of fluorophore with unique photoluminescence properties, in particular, CdSe/ZnS core-shell QDs have been successfully used as biocompatible fluorescence resonance energy transfer donors. Here we report FRET between CdSe/ZnS core-shell QDs (donor) and organic dye fluorescein 27 (F27) (acceptor). The results demonstrate the occurrence of efficient energy transfer in the system and the FRET efficiency is not only influenced by the spectral overlap between the QD donor emission and acceptor absorption, it might depend on QDs surface effect also. Efforts are made to correlate quantitatively spectral dependence of FRET rate with acceptor absorption spectrum, Forster distance, transfer efficiency (E) obtained employing steady-state & time-resolved technique.
基金The authors greatly appreciate the-nancial support from National Natural Science Foundation of China(Grant Nos.21522402,11674153,11374148,11334004 and 21771103)the Fundamental Research Funds for the Central Universities(Nos.020414380070,020414380050 and 020414380058)Natural Science Foundation of Jiangsu Province(No.BK20160639)and the Shuangchuang Program of Jiangsu Province.
文摘Enzymes are the major players for many biological processes.Fundamental studies of the enzymatic activity at the single-molecule level provides important information that is otherwise inaccessible at the ensemble level.Yet,these single-molecule experiments are technically di±cult and generally require complicated experimental design.Here,we develop a Holliday junction(HJ)-based platform to study the activity of restriction endonucleases at the single-molecule level using single-molecule FRET(sm-FRET).We show that the intrinsic dynamics of HJ can be used as the reporter for both the enzyme-binding and the substrate-release events.Thanks to the multiple-arms structure of HJ,the fluorophore-labeled arms can be different from the surface anchoring arm and the substrate arm.Therefore,it is possible to independently change the substrate arm to study different enzymes with similar functions.Such a design is extremely useful for the systematic study of enzymes from the same family or enzymes bearing different pathologic mutations.Moreover,this method can be easily extended to study other types of DNA-binding enzymes without too much modi fication of the design.We anticipate it can find broad applications in single-molecule enzymology.
文摘In the course of pushing forward the overall growth capacities of the mining industry and getting adapted to the tense competition after China抯 entry into WTO, it is quite necessary to speed up the process of fostering large state-owned coal group enter-prises. This strategical proposal is made on the basis of a comprehensive analysis of the main problems challenges faced by the coal mining industry is made on. The main prob-lems with Chinese coal mining industries discussed include: over-competition with a low convergence, severe waste and loss of mine resources with poor exploitation techniques, disordered operation with few industrial entry barriers, heavy welfare burdens from low socialization. The accession of WTO provide Chinese coal mining industry with chances to push forward the reforms and structural shift in the enterprises, expedite the estab-lishment and perfect of coal mining market mechanism, expand their international market share as well as introduce foreign investment, and with challenges from high technology, knowledge economy, and information revolution. The solutions to the foster of large-scale coal mining group enterprises, as well as the possible points to note and the policy pro-posal are advanced.
文摘W942715 小麦族耐胁迫性的遗传工程[刊,英]/Forster,B.P.//Proceedings of the Royal Society ofEdinburgh.Section B,Biological Sciences.-1992,99(3/4).-89～106[WBTA,1993,10(3).2550]W942716 入选捷克斯洛伐克春大麦品种对白粉病的抗性[刊,捷]/Dreiseitl Antonin…//Genet.aslecht..-1993.29(2).-123～130 [493,1993,(11/12),176]W942717 捷克斯洛伐克春大麦品种对白粉病抗性的评价[刊,捷]/Dreiseitl,A.//Rostl.vyroba.-1993,39(4).-337～344[484,1993,(11/12).153]
文摘《阜阳职业技术学院学报》2023年第2期第66-70页刊发刘知国撰写的“生态女性主义视角下的爱德华·福斯特小说研究”一文,英文目次页中题名译文为“A Study of Edward Forster's Novels from the Perspective of Ecofeminism”,现更改为“Exploration on E·M·Forster’s Novels in Perspective of Ecofeminism”。特此声明。
文摘This paper analyses the female characters in E.M.Forster’s novels from the perspective of feminism,through the deep exploration of E.M.Forster’s works,it is evident to draw a conclusion that there are four kinds of female characters:first of all,stubborn and arrogant old ladies;the second type is insensitive but self-awakening middle-aged ladies;the third type is knowledgeable and smart young ladies;the last one is the warm-hearted and benevolent lady.These four kinds of different female characters can reveal that E.M.Forster has a contradictory attitude towards women.For one thing,he respected and sympathized with women,for another he discriminated against women.
文摘In Forster's masterpiece A Passage to India, the novelist deals in large parts with the contemporary Indian society under British rule and the complicated relations between the English and the native population. In this novel, Forster explores the difficulty to unify human relations,and exposes the eternal gaps between two nations. This paper will center on the eternal gaps of a Passage to India, which are represented by the misunderstandings between"India"and"British". By exploring and analyzing those gaps, the paper reaches the conclusion that those gaps can not be eliminated and the long-existing communication proposed by Forster is still a long way to go.
